Restriction map for
pF1KB7398
(Circular) (Six-base) MAPSORT of: pF1KB7398.seq Check: 5141 from: 1 to: 4088
pF1KB7398 4088 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 381 381
Size: 4088
AccI GT'mk_AC
Cuts at: 1126 1126
Size: 4088
Acc65I G'GTAC_C
Cuts at: 736 847 1104 736
Size: 111 257 3720
Fragments arranged by size:
3720 257 111
AclI AA'CG_TT
Cuts at: 3928 3928
Size: 4088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 524 1913 2345 3092 3360 358
Size: 166 1389 432 747 268 1086
Fragments arranged by size:
1389 1086 747 432 268 166
AfeI AGC'GCT
Cuts at: 497 497
Size: 4088
AflIII A'CryG_T
Cuts at: 1440 2544 3521 1440
Size: 1104 977 2007
Fragments arranged by size:
2007 1104 977
AgeI A'CCGG_T
Cuts at: 2476 2476
Size: 4088
AhdI GACnn_n'nnGTC
Cuts at: 309 627 309
Size: 318 3770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 147 147
Size: 4088
AlwNI CAG_nnn'CTG
Cuts at: 1393 2960 1393
Size: 1567 2521
ApaLI G'TGCA_C
Cuts at: 2858 2858
Size: 4088
ApoI r'AATT_y
Cuts at: 1092 3441 3697 1092
Size: 2349 256 1483
Fragments arranged by size:
2349 1483 256
AseI AT'TA_AT
Cuts at: 20 866 899 932 20
Size: 846 33 33 3176
Fragments arranged by size:
3176 846 33 33
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
AvaI C'yCGr_G
Cuts at: 1108 1108
Size: 4088
AvrII C'CTAG_G
Cuts at: 307 1066 307
Size: 759 3329
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 963 996 963
Size: 33 4055
BamHI G'GATC_C
Cuts at: 447 1113 447
Size: 666 3422
BanI G'GyrC_C
Cuts at: 736 847 1104 1518 1766 1801 736
Size: 111 257 414 248 35 3023
Fragments arranged by size:
3023 414 257 248 111 35
BanII G_rGCy'C
Cuts at: 1102 2132 1102
Size: 1030 3058
BbeI G_GCGC'C
Cuts at: 1522 1770 1522
Size: 248 3840
BbsI GAAGACnn'nnnn_
Cuts at: 154 563 154
Size: 409 3679
BbvCI CC'TCA_GC
Cuts at: 242 476 242
Size: 234 3854
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 783 817 90
Size: 34 659 34 3361
Fragments arranged by size:
3361 659 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 884 917 950 1986 2747 4088 884
Size: 33 33 1036 761 1341 884
Fragments arranged by size:
1341 1036 884 761 33 33
BclI T'GATC_A
Cuts at: 723 1608 723
Size: 885 3203
BglI GCCn_nnn'nGGC
Cuts at: 1019 1019
Size: 4088
BglII A'GATC_T
Cuts at: 1603 3640 1603
Size: 2037 2051
BlpI GC'TnA_GC
Cuts at: 343 1200 343
Size: 857 3231
Bme1580I G_kGCm'C
Cuts at: 1713 1806 2862 1713
Size: 93 1056 2939
Fragments arranged by size:
2939 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 667 743 1300 1381 1716 3860 667
Size: 76 557 81 335 2144 895
Fragments arranged by size:
2144 895 557 335 81 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 152 419 2455 152
Size: 267 2036 1785
Fragments arranged by size:
2036 1785 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 1022 507
Size: 515 3573
Bpu10I CC'TnA_GC
Cuts at: 242 476 242
Size: 234 3854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 2143 2635 2933 1264
Size: 879 492 298 2419
Fragments arranged by size:
2419 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 704 3792 704
Size: 3088 1000
BsaAI yAC'GTr
Cuts at: 1441 1441
Size: 4088
BsaBI GATnn'nnATC
Cuts at: 6 1602 3639 6
Size: 1596 2037 455
Fragments arranged by size:
2037 1596 455
BsaHI Gr'CG_yC
Cuts at: 1519 1767 2469 3965 1519
Size: 248 702 1496 1642
Fragments arranged by size:
1642 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1277 1798 2476 2750 2897 3308 1277
Size: 521 678 274 147 411 2057
Fragments arranged by size:
2057 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 180 210 180
Size: 30 4058
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 128 220 601 128
Size: 92 381 3615
Fragments arranged by size:
3615 381 92
BseYI C'CCAG_C
Cuts at: 854 887 920 953 1038 1513 2271 2848 3344
Size: 33 33 33 85 475 758 577 496
Cuts at: 3344 854
Size: 1598
Fragments arranged by size:
1598 758 577 496 475 85 33 33
33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 4088
BsiEI CG_ry'CG
Cuts at: 104 1630 1676 2884 104
Size: 1526 46 1208 1308
Fragments arranged by size:
1526 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1102 1880 2070 2862 1102
Size: 778 190 792 2328
Fragments arranged by size:
2328 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1102 1713 1806 1880 2070 2132 2862 1102
Size: 611 93 74 190 62 730 2328
Fragments arranged by size:
2328 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3308 3308
Size: 4088
BspHI T'CATG_A
Cuts at: 3264 3264
Size: 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 381 1138 1352 1654 2035 381
Size: 757 214 302 381 2434
Fragments arranged by size:
2434 757 381 302 214
BsrBI CCG'CTC
Cuts at: 50 298 2380 2434 3918 4083 50
Size: 248 2082 54 1484 165 55
Fragments arranged by size:
2082 1484 248 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2000 2000
Size: 4088
BsrFI r'CCGG_y
Cuts at: 2086 2267 2476 2086
Size: 181 209 3698
Fragments arranged by size:
3698 209 181
BssSI C'ACGA_G
Cuts at: 2359 2717 2359
Size: 358 3730
BstAPI GCAn_nnn'nTGC
Cuts at: 1465 1465
Size: 4088
BstBI TT'CG_AA
Cuts at: 2449 2449
Size: 4088
BstYI r'GATC_y
Cuts at: 1 447 1113 1580 1603 1938 2184 3196 3282
Size: 446 666 467 23 335 246 1012 86
Cuts at: 3282 3294 3640 1
Size: 12 346 449
Fragments arranged by size:
1012 666 467 449 446 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 443 812 443
Size: 369 3719
BtgI C'CryG_G
Cuts at: 425 425
Size: 4088
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2038 2219 2382 2038
Size: 181 163 3744
Fragments arranged by size:
3744 181 163
BtsI GCAGTG_nn'
Cuts at: 395 555 1081 1331 395
Size: 160 526 250 3152
Fragments arranged by size:
3152 526 250 160
DraI TTT'AAA
Cuts at: 1087 1087
Size: 4088
DrdI GACnn_nn'nnGTC
Cuts at: 1794 2652 1794
Size: 858 3230
EaeI y'GGCC_r
Cuts at: 1673 1847 2238 2265 3610 1673
Size: 174 391 27 1345 2151
Fragments arranged by size:
2151 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1673 1673
Size: 4088
EarI CTCTTCn'nnn_
Cuts at: 1029 2111 2321 1029
Size: 1082 210 2796
Fragments arranged by size:
2796 1082 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1000 2296 2606 2752 3898 1000
Size: 1296 310 146 1146 1190
Fragments arranged by size:
1296 1190 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1100 1100
Size: 4088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 507 524 1022 1913 2345 3092 3360 358
Size: 149 17 498 891 432 747 268 1086
Fragments arranged by size:
1086 891 747 498 432 268 149 17
EcoNI CCTnn'n_nnAGG
Cuts at: 377 616 377
Size: 239 3849
EcoO109I rG'GnC_Cy
Cuts at: 772 1227 772
Size: 455 3633
EcoRI G'AATT_C
Cuts at: 1092 1092
Size: 4088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 4088
FspI TGC'GCA
Cuts at: 1869 1869
Size: 4088
HaeII r_GCGC'y
Cuts at: 499 1522 1770 2792 3771 499
Size: 1023 248 1022 979 816
Fragments arranged by size:
1023 1022 979 816 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 613 645 1930 1960 1962 1992 295
Size: 32 286 32 1285 30 2 30 2391
Fragments arranged by size:
2391 1285 286 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1127 1127
Size: 4088
KasI G'GCGC_C
Cuts at: 1518 1766 1518
Size: 248 3840
KpnI G_GTAC'C
Cuts at: 740 851 1108 740
Size: 111 257 3720
Fragments arranged by size:
3720 257 111
MluI A'CGCG_T
Cuts at: 3521 3521
Size: 4088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2759 2943 2759
Size: 184 3904
MscI TGG'CCA
Cuts at: 1849 1849
Size: 4088
MslI CAynn'nnrTG
Cuts at: 2077 2204 2077
Size: 127 3961
MspA1I CmG'CkG
Cuts at: 854 887 920 953 1042 1199 1513 1873 2886
Size: 33 33 33 89 157 314 360 1013
Cuts at: 2886 3131 854
Size: 245 1811
Fragments arranged by size:
1811 1013 360 314 245 157 89 33
33 33
NaeI GCC'GGC
Cuts at: 2269 2269
Size: 4088
NarI GG'CG_CC
Cuts at: 1519 1767 1519
Size: 248 3840
NcoI C'CATG_G
Cuts at: 425 425
Size: 4088
NgoMIV G'CCGG_C
Cuts at: 2267 2267
Size: 4088
NspI r_CATG'y
Cuts at: 1141 1641 2548 1141
Size: 500 907 2681
Fragments arranged by size:
2681 907 500
PasI CC'CwG_GG
Cuts at: 731 775 731
Size: 44 4044
PciI A'CATG_T
Cuts at: 2544 2544
Size: 4088
PflMI CCAn_nnn'nTGG
Cuts at: 225 225
Size: 4088
PfoI T'CCnGG_A
Cuts at: 876 909 942 999 876
Size: 33 33 57 3965
Fragments arranged by size:
3965 57 33 33
PmeI GTTT'AAAC
Cuts at: 1087 1087
Size: 4088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 4056
PstI C_TGCA'G
Cuts at: 378 390 1135 1820 378
Size: 12 745 685 2646
Fragments arranged by size:
2646 745 685 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4088
PvuII CAG'CTG
Cuts at: 854 887 920 953 1042 1513 1873 854
Size: 33 33 33 89 471 360 3069
Fragments arranged by size:
3069 471 360 89 33 33 33
RsrII CG'GwC_CG
Cuts at: 2283 2283
Size: 4088
SacI G_AGCT'C
Cuts at: 1102 1102
Size: 4088
SalI G'TCGA_C
Cuts at: 1125 1125
Size: 4088
SapI GCTCTTCn'nnn_
Cuts at: 1029 2111 2321 1029
Size: 1082 210 2796
Fragments arranged by size:
2796 1082 210
SbfI CC_TGCA'GG
Cuts at: 378 1135 378
Size: 757 3331
ScaI AGT'ACT
Cuts at: 3605 3605
Size: 4088
SfcI C'TryA_G
Cuts at: 33 374 386 1131 1816 2809 3000 33
Size: 341 12 745 685 993 191 1121
Fragments arranged by size:
1121 993 745 685 341 191 12
SfoI GGC'GCC
Cuts at: 1520 1768 1520
Size: 248 3840
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
SmaI CCC'GGG
Cuts at: 1110 1110
Size: 4088
SmlI C'TyrA_G
Cuts at: 1243 2158 2650 2912 1243
Size: 915 492 262 2419
Fragments arranged by size:
2419 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4088
SphI G_CATG'C
Cuts at: 1141 1641 1141
Size: 500 3588
SspI AAT'ATT
Cuts at: 864 897 930 963 864
Size: 33 33 33 3989
Fragments arranged by size:
3989 33 33 33
StuI AGG'CCT
Cuts at: 176 590 176
Size: 414 3674
StyI C'CwwG_G
Cuts at: 307 425 451 599 1066 1222 1576 307
Size: 118 26 148 467 156 354 2819
Fragments arranged by size:
2819 467 354 156 148 118 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 612 1671 1804 2263 2472 612
Size: 1059 133 459 209 2228
Fragments arranged by size:
2228 1059 459 209 133
TatI w'GTAC_w
Cuts at: 3603 3603
Size: 4088
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 180 212 3605 3637 180
Size: 32 3393 32 631
Fragments arranged by size:
3393 631 32 32
Tth111I GACn'n_nGTC
Cuts at: 1885 1885
Size: 4088
XbaI T'CTAG_A
Cuts at: 1119 1119
Size: 4088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 851 884 917 950 1583 851
Size: 33 33 33 633 3356
Fragments arranged by size:
3356 633 33 33 33
XmaI C'CCGG_G
Cuts at: 1108 1108
Size: 4088
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AloI ApaI AscI BfrBI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI