Restriction map for
pF1KB7399
(Circular) (Six-base) MAPSORT of: pF1KB7399.seq Check: 9283 from: 1 to: 3641
pF1KB7399 3641 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 679 679
Size: 3641
Acc65I G'GTAC_C
Cuts at: 657 657
Size: 3641
AclI AA'CG_TT
Cuts at: 3481 3481
Size: 3641
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 1466 1898 2645 2913 471
Size: 995 432 747 268 1199
Fragments arranged by size:
1199 995 747 432 268
AflIII A'CryG_T
Cuts at: 993 2097 3074 993
Size: 1104 977 1560
Fragments arranged by size:
1560 1104 977
AgeI A'CCGG_T
Cuts at: 2029 2029
Size: 3641
AlwNI CAG_nnn'CTG
Cuts at: 946 2513 946
Size: 1567 2074
ApaLI G'TGCA_C
Cuts at: 2411 2411
Size: 3641
ApoI r'AATT_y
Cuts at: 645 2994 3250 645
Size: 2349 256 1036
Fragments arranged by size:
2349 1036 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3641
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3641
AvaI C'yCGr_G
Cuts at: 661 661
Size: 3641
BamHI G'GATC_C
Cuts at: 666 666
Size: 3641
BanI G'GyrC_C
Cuts at: 657 1071 1319 1354 657
Size: 414 248 35 2944
Fragments arranged by size:
2944 414 248 35
BanII G_rGCy'C
Cuts at: 613 655 1685 613
Size: 42 1030 2569
Fragments arranged by size:
2569 1030 42
BbeI G_GCGC'C
Cuts at: 1075 1323 1075
Size: 248 3393
BbsI GAAGACnn'nnnn_
Cuts at: 273 388 273
Size: 115 3526
BciVI GTATCCnnnnn_n'
Cuts at: 1539 2300 3641 1539
Size: 761 1341 1539
Fragments arranged by size:
1539 1341 761
BclI T'GATC_A
Cuts at: 1161 1161
Size: 3641
BglII A'GATC_T
Cuts at: 1156 3193 1156
Size: 2037 1604
BlpI GC'TnA_GC
Cuts at: 124 136 753 124
Size: 12 617 3012
Fragments arranged by size:
3012 617 12
Bme1580I G_kGCm'C
Cuts at: 1266 1359 2415 1266
Size: 93 1056 2492
Fragments arranged by size:
2492 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 853 934 1269 3413 853
Size: 81 335 2144 1081
Fragments arranged by size:
2144 1081 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2008 2008
Size: 3641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 463
Size: 3641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1696 2188 2486 817
Size: 879 492 298 1972
Fragments arranged by size:
1972 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 263 496 3345 263
Size: 233 2849 559
Fragments arranged by size:
2849 559 233
BsaAI yAC'GTr
Cuts at: 994 994
Size: 3641
BsaBI GATnn'nnATC
Cuts at: 6 1155 3192 6
Size: 1149 2037 455
Fragments arranged by size:
2037 1149 455
BsaHI Gr'CG_yC
Cuts at: 1072 1320 2022 3518 1072
Size: 248 702 1496 1195
Fragments arranged by size:
1496 1195 702 248
BsaWI w'CCGG_w
Cuts at: 287 830 1351 2029 2303 2450 2861 287
Size: 543 521 678 274 147 411 1067
Fragments arranged by size:
1067 678 543 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1066 1824 2401 2897 1066
Size: 758 577 496 1810
Fragments arranged by size:
1810 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 364
Size: 3641
BsiEI CG_ry'CG
Cuts at: 104 1183 1229 2437 104
Size: 1079 46 1208 1308
Fragments arranged by size:
1308 1208 1079 46
BsiHKAI G_wGCw'C
Cuts at: 655 1433 1623 2415 655
Size: 778 190 792 1881
Fragments arranged by size:
1881 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 444 444
Size: 3641
Bsp1286I G_dGCh'C
Cuts at: 613 655 1266 1359 1433 1623 1685 2415 613
Size: 42 611 93 74 190 62 730 1839
Fragments arranged by size:
1839 730 611 190 93 74 62 42
BspEI T'CCGG_A
Cuts at: 287 2861 287
Size: 2574 1067
BspHI T'CATG_A
Cuts at: 2817 2817
Size: 3641
BspMI ACCTGCnnnn'nnnn_
Cuts at: 538 691 905 1207 1588 538
Size: 153 214 302 381 2591
Fragments arranged by size:
2591 381 302 214 153
BsrBI CCG'CTC
Cuts at: 50 1933 1987 3471 3636 50
Size: 1883 54 1484 165 55
Fragments arranged by size:
1883 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1553 1553
Size: 3641
BsrFI r'CCGG_y
Cuts at: 1639 1820 2029 1639
Size: 181 209 3251
Fragments arranged by size:
3251 209 181
BssSI C'ACGA_G
Cuts at: 1912 2270 1912
Size: 358 3283
BstAPI GCAn_nnn'nTGC
Cuts at: 1018 1018
Size: 3641
BstBI TT'CG_AA
Cuts at: 332 2002 332
Size: 1670 1971
BstYI r'GATC_y
Cuts at: 1 582 666 1133 1156 1491 1737 2749 2835
Size: 581 84 467 23 335 246 1012 86
Cuts at: 2835 2847 3193 1
Size: 12 346 449
Fragments arranged by size:
1012 581 467 449 346 335 246 86
84 23 12
Bsu36I CC'TnA_GG
Cuts at: 274 274
Size: 3641
BtgI C'CryG_G
Cuts at: 107 107
Size: 3641
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 129 1591 1772 1935 129
Size: 1462 181 163 1835
Fragments arranged by size:
1835 1462 181 163
BtsI GCAGTG_nn'
Cuts at: 884 884
Size: 3641
DraI TTT'AAA
Cuts at: 640 640
Size: 3641
DrdI GACnn_nn'nnGTC
Cuts at: 277 1347 2205 277
Size: 1070 858 1713
Fragments arranged by size:
1713 1070 858
EaeI y'GGCC_r
Cuts at: 197 434 1226 1400 1791 1818 3163 197
Size: 237 792 174 391 27 1345 675
Fragments arranged by size:
1345 792 675 391 237 174 27
EagI C'GGCC_G
Cuts at: 1226 1226
Size: 3641
EarI CTCTTCn'nnn_
Cuts at: 1664 1874 1664
Size: 210 3431
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1849 2159 2305 3451 1849
Size: 310 146 1146 2039
Fragments arranged by size:
2039 1146 310 146
EcoICRI GAG'CTC
Cuts at: 653 653
Size: 3641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 471 1466 1898 2645 2913 463
Size: 8 995 432 747 268 1191
Fragments arranged by size:
1191 995 747 432 268 8
EcoO109I rG'GnC_Cy
Cuts at: 780 780
Size: 3641
EcoRI G'AATT_C
Cuts at: 645 645
Size: 3641
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1660 1660
Size: 3641
FspI TGC'GCA
Cuts at: 1422 1422
Size: 3641
HaeII r_GCGC'y
Cuts at: 520 1075 1323 2345 3324 520
Size: 555 248 1022 979 837
Fragments arranged by size:
1022 979 837 555 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1483 1513 1515 1545 1483
Size: 30 2 30 3579
Fragments arranged by size:
3579 30 30 2
HincII GTy'rAC
Cuts at: 680 680
Size: 3641
KasI G'GCGC_C
Cuts at: 1071 1319 1071
Size: 248 3393
KpnI G_GTAC'C
Cuts at: 661 661
Size: 3641
MluI A'CGCG_T
Cuts at: 3074 3074
Size: 3641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2312 2496 2312
Size: 184 3457
MscI TGG'CCA
Cuts at: 199 1402 199
Size: 1203 2438
MslI CAynn'nnrTG
Cuts at: 175 1630 1757 175
Size: 1455 127 2059
Fragments arranged by size:
2059 1455 127
MspA1I CmG'CkG
Cuts at: 752 1066 1426 2439 2684 752
Size: 314 360 1013 245 1709
Fragments arranged by size:
1709 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1822 1822
Size: 3641
NarI GG'CG_CC
Cuts at: 1072 1320 1072
Size: 248 3393
NcoI C'CATG_G
Cuts at: 107 107
Size: 3641
NgoMIV G'CCGG_C
Cuts at: 1820 1820
Size: 3641
NspI r_CATG'y
Cuts at: 694 1194 2101 694
Size: 500 907 2234
Fragments arranged by size:
2234 907 500
PciI A'CATG_T
Cuts at: 2097 2097
Size: 3641
PflMI CCAn_nnn'nTGG
Cuts at: 513 632 513
Size: 119 3522
PmeI GTTT'AAAC
Cuts at: 640 640
Size: 3641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2780 2812 2780
Size: 32 3609
PstI C_TGCA'G
Cuts at: 688 1373 688
Size: 685 2956
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3641
PvuII CAG'CTG
Cuts at: 1066 1426 1066
Size: 360 3281
RsrII CG'GwC_CG
Cuts at: 1836 1836
Size: 3641
SacI G_AGCT'C
Cuts at: 655 655
Size: 3641
SalI G'TCGA_C
Cuts at: 678 678
Size: 3641
SapI GCTCTTCn'nnn_
Cuts at: 1664 1874 1664
Size: 210 3431
SbfI CC_TGCA'GG
Cuts at: 688 688
Size: 3641
ScaI AGT'ACT
Cuts at: 3158 3158
Size: 3641
SfcI C'TryA_G
Cuts at: 33 453 684 1369 2362 2553 33
Size: 420 231 685 993 191 1121
Fragments arranged by size:
1121 993 685 420 231 191
SfoI GGC'GCC
Cuts at: 1073 1321 1073
Size: 248 3393
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3641
SmaI CCC'GGG
Cuts at: 663 663
Size: 3641
SmlI C'TyrA_G
Cuts at: 796 1711 2203 2465 796
Size: 915 492 262 1972
Fragments arranged by size:
1972 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3641
SphI G_CATG'C
Cuts at: 694 1194 694
Size: 500 3141
SspI AAT'ATT
Cuts at: 294 323 294
Size: 29 3612
StuI AGG'CCT
Cuts at: 621 621
Size: 3641
StyI C'CwwG_G
Cuts at: 107 775 1129 107
Size: 668 354 2619
Fragments arranged by size:
2619 668 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 514 1224 1357 1816 2025 514
Size: 710 133 459 209 2130
Fragments arranged by size:
2130 710 459 209 133
TatI w'GTAC_w
Cuts at: 3156 3156
Size: 3641
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3609
Tth111I GACn'n_nGTC
Cuts at: 1438 1438
Size: 3641
XbaI T'CTAG_A
Cuts at: 672 672
Size: 3641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1136 1136
Size: 3641
XmaI C'CCGG_G
Cuts at: 661 661
Size: 3641
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI