Restriction map for
pF1KB7402
(Circular) (Six-base) MAPSORT of: pF1KB7402.seq Check: 7645 from: 1 to: 4202
pF1KB7402 4202 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1240 1240
Size: 4202
Acc65I G'GTAC_C
Cuts at: 573 1218 573
Size: 645 3557
AclI AA'CG_TT
Cuts at: 4042 4042
Size: 4202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2459 3206 3474 2027
Size: 432 747 268 2755
Fragments arranged by size:
2755 747 432 268
AflII C'TTAA_G
Cuts at: 322 322
Size: 4202
AflIII A'CryG_T
Cuts at: 1554 2658 3635 1554
Size: 1104 977 2121
Fragments arranged by size:
2121 1104 977
AgeI A'CCGG_T
Cuts at: 2590 2590
Size: 4202
AlwNI CAG_nnn'CTG
Cuts at: 627 1507 3074 627
Size: 880 1567 1755
Fragments arranged by size:
1755 1567 880
ApaI G_GGCC'C
Cuts at: 835 835
Size: 4202
ApaLI G'TGCA_C
Cuts at: 2972 2972
Size: 4202
ApoI r'AATT_y
Cuts at: 948 1206 3555 3811 948
Size: 258 2349 256 1339
Fragments arranged by size:
2349 1339 258 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4202
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
AvaI C'yCGr_G
Cuts at: 130 1222 130
Size: 1092 3110
BamHI G'GATC_C
Cuts at: 1227 1227
Size: 4202
BanI G'GyrC_C
Cuts at: 207 546 573 649 1218 1632 1880 1915 207
Size: 339 27 76 569 414 248 35 2494
Fragments arranged by size:
2494 569 414 339 248 76 35 27
BanII G_rGCy'C
Cuts at: 835 975 1216 2246 835
Size: 140 241 1030 2791
Fragments arranged by size:
2791 1030 241 140
BbeI G_GCGC'C
Cuts at: 1636 1884 1636
Size: 248 3954
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 729 763 90
Size: 34 605 34 3529
Fragments arranged by size:
3529 605 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 897 2100 2861 4202 897
Size: 1203 761 1341 897
Fragments arranged by size:
1341 1203 897 761
BclI T'GATC_A
Cuts at: 341 1722 341
Size: 1381 2821
BglII A'GATC_T
Cuts at: 1717 3754 1717
Size: 2037 2165
BlpI GC'TnA_GC
Cuts at: 751 1314 751
Size: 563 3639
Bme1580I G_kGCm'C
Cuts at: 818 835 1827 1920 2976 818
Size: 17 992 93 1056 2044
Fragments arranged by size:
2044 1056 992 93 17
BmrI ACTGGGnnnn_n'
Cuts at: 1414 1495 1830 3974 1414
Size: 81 335 2144 1642
Fragments arranged by size:
2144 1642 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 4202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 844 1006 165
Size: 679 162 3361
Fragments arranged by size:
3361 679 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1378 2257 2749 3047 235
Size: 1143 879 492 298 1390
Fragments arranged by size:
1390 1143 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 271 3906 271
Size: 3635 567
BsaAI yAC'GTr
Cuts at: 227 1555 227
Size: 1328 2874
BsaBI GATnn'nnATC
Cuts at: 6 790 1716 3753 6
Size: 784 926 2037 455
Fragments arranged by size:
2037 926 784 455
BsaHI Gr'CG_yC
Cuts at: 1633 1881 2583 4079 1633
Size: 248 702 1496 1756
Fragments arranged by size:
1756 1496 702 248
BsaWI w'CCGG_w
Cuts at: 635 1391 1912 2590 2864 3011 3422 635
Size: 756 521 678 274 147 411 1415
Fragments arranged by size:
1415 756 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 421 451 646 676 856 886 421
Size: 30 195 30 180 30 3737
Fragments arranged by size:
3737 195 180 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 324 892 324
Size: 568 3634
BseYI C'CCAG_C
Cuts at: 1627 2385 2962 3458 1627
Size: 758 577 496 2371
Fragments arranged by size:
2371 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 957 459
Size: 498 3704
BsiEI CG_ry'CG
Cuts at: 104 1744 1790 2998 104
Size: 1640 46 1208 1308
Fragments arranged by size:
1640 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 462 1216 1994 2184 2976 462
Size: 754 778 190 792 1688
Fragments arranged by size:
1688 792 778 754 190
Bsp1286I G_dGCh'C
Cuts at: 462 818 835 975 1216 1827 1920 1994 2184
Size: 356 17 140 241 611 93 74 190
Cuts at: 2184 2246 2976 462
Size: 62 730 1688
Fragments arranged by size:
1688 730 611 356 241 190 140 93
74 62 17
BspEI T'CCGG_A
Cuts at: 3422 3422
Size: 4202
BspHI T'CATG_A
Cuts at: 842 3378 842
Size: 2536 1666
BspMI ACCTGCnnnn'nnnn_
Cuts at: 345 1252 1466 1768 2149 345
Size: 907 214 302 381 2398
Fragments arranged by size:
2398 907 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2494 2548 4032 4197 50
Size: 2444 54 1484 165 55
Fragments arranged by size:
2444 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1175 2114 1175
Size: 939 3263
BsrFI r'CCGG_y
Cuts at: 2200 2381 2590 2200
Size: 181 209 3812
Fragments arranged by size:
3812 209 181
BsrGI T'GTAC_A
Cuts at: 377 377
Size: 4202
BssSI C'ACGA_G
Cuts at: 2473 2831 2473
Size: 358 3844
BstAPI GCAn_nnn'nTGC
Cuts at: 1579 1579
Size: 4202
BstBI TT'CG_AA
Cuts at: 2563 2563
Size: 4202
BstYI r'GATC_y
Cuts at: 1 362 485 1227 1694 1717 2052 2298 3310
Size: 361 123 742 467 23 335 246 1012
Cuts at: 3310 3396 3408 3754 1
Size: 86 12 346 449
Fragments arranged by size:
1012 742 467 449 361 346 335 246
123 86 23 12
BtgI C'CryG_G
Cuts at: 975 975
Size: 4202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2152 2333 2496 2152
Size: 181 163 3858
Fragments arranged by size:
3858 181 163
BtsI GCAGTG_nn'
Cuts at: 447 1445 447
Size: 998 3204
DraI TTT'AAA
Cuts at: 1201 1201
Size: 4202
DraIII CAC_nnn'GTG
Cuts at: 227 1189 227
Size: 962 3240
DrdI GACnn_nn'nnGTC
Cuts at: 1908 2766 1908
Size: 858 3344
EaeI y'GGCC_r
Cuts at: 776 956 1787 1961 2352 2379 3724 776
Size: 180 831 174 391 27 1345 1254
Fragments arranged by size:
1345 1254 831 391 180 174 27
EagI C'GGCC_G
Cuts at: 1787 1787
Size: 4202
EarI CTCTTCn'nnn_
Cuts at: 2225 2435 2225
Size: 210 3992
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 604 2410 2720 2866 4012 604
Size: 1806 310 146 1146 794
Fragments arranged by size:
1806 1146 794 310 146
EcoICRI GAG'CTC
Cuts at: 1214 1214
Size: 4202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 844 1006 2027 2459 3206 3474 165
Size: 679 162 1021 432 747 268 893
Fragments arranged by size:
1021 893 747 679 432 268 162
EcoNI CCTnn'n_nnAGG
Cuts at: 826 919 826
Size: 93 4109
EcoO109I rG'GnC_Cy
Cuts at: 831 832 1341 831
Size: 1 509 3692
Fragments arranged by size:
3692 509 1
EcoRI G'AATT_C
Cuts at: 1206 1206
Size: 4202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 4202
FspI TGC'GCA
Cuts at: 1983 1983
Size: 4202
HaeII r_GCGC'y
Cuts at: 1636 1884 2906 3885 1636
Size: 248 1022 979 1953
Fragments arranged by size:
1953 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 251 283 871 903 1146 1178 2044 2074 2076
Size: 32 588 32 243 32 866 30 2
Cuts at: 2076 2106 251
Size: 30 2347
Fragments arranged by size:
2347 866 588 243 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1241 1241
Size: 4202
KasI G'GCGC_C
Cuts at: 1632 1880 1632
Size: 248 3954
KpnI G_GTAC'C
Cuts at: 577 1222 577
Size: 645 3557
MluI A'CGCG_T
Cuts at: 3635 3635
Size: 4202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2873 3057 2873
Size: 184 4018
MscI TGG'CCA
Cuts at: 778 958 1963 778
Size: 180 1005 3017
Fragments arranged by size:
3017 1005 180
MslI CAynn'nnrTG
Cuts at: 112 199 622 2191 2318 112
Size: 87 423 1569 127 1996
Fragments arranged by size:
1996 1569 423 127 87
MspA1I CmG'CkG
Cuts at: 1313 1627 1987 3000 3245 1313
Size: 314 360 1013 245 2270
Fragments arranged by size:
2270 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2383 2383
Size: 4202
NarI GG'CG_CC
Cuts at: 1633 1881 1633
Size: 248 3954
NgoMIV G'CCGG_C
Cuts at: 2381 2381
Size: 4202
NspI r_CATG'y
Cuts at: 1255 1755 2662 1255
Size: 500 907 2795
Fragments arranged by size:
2795 907 500
PciI A'CATG_T
Cuts at: 2658 2658
Size: 4202
PfoI T'CCnGG_A
Cuts at: 512 821 983 512
Size: 309 162 3731
Fragments arranged by size:
3731 309 162
PmeI GTTT'AAAC
Cuts at: 1201 1201
Size: 4202
PmlI CAC'GTG
Cuts at: 227 227
Size: 4202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 4170
PspOMI G'GGCC_C
Cuts at: 831 831
Size: 4202
PstI C_TGCA'G
Cuts at: 1249 1934 1249
Size: 685 3517
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4202
PvuII CAG'CTG
Cuts at: 1627 1987 1627
Size: 360 3842
RsrII CG'GwC_CG
Cuts at: 2397 2397
Size: 4202
SacI G_AGCT'C
Cuts at: 1216 1216
Size: 4202
SalI G'TCGA_C
Cuts at: 1239 1239
Size: 4202
SapI GCTCTTCn'nnn_
Cuts at: 2225 2435 2225
Size: 210 3992
SbfI CC_TGCA'GG
Cuts at: 1249 1249
Size: 4202
ScaI AGT'ACT
Cuts at: 3719 3719
Size: 4202
SfcI C'TryA_G
Cuts at: 33 501 1245 1930 2923 3114 33
Size: 468 744 685 993 191 1121
Fragments arranged by size:
1121 993 744 685 468 191
SfoI GGC'GCC
Cuts at: 1634 1882 1634
Size: 248 3954
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
SmaI CCC'GGG
Cuts at: 1224 1224
Size: 4202
SmlI C'TyrA_G
Cuts at: 250 322 1357 2272 2764 3026 250
Size: 72 1035 915 492 262 1426
Fragments arranged by size:
1426 1035 915 492 262 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4202
SphI G_CATG'C
Cuts at: 1255 1755 1255
Size: 500 3702
StyI C'CwwG_G
Cuts at: 401 522 665 867 1336 1690 401
Size: 121 143 202 469 354 2913
Fragments arranged by size:
2913 469 354 202 143 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1094 1785 1918 2377 2586 1094
Size: 691 133 459 209 2710
Fragments arranged by size:
2710 691 459 209 133
TatI w'GTAC_w
Cuts at: 377 3717 377
Size: 3340 862
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 421 453 3719 3751 421
Size: 32 3266 32 872
Fragments arranged by size:
3266 872 32 32
Tth111I GACn'n_nGTC
Cuts at: 263 1999 263
Size: 1736 2466
XbaI T'CTAG_A
Cuts at: 1233 1233
Size: 4202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 408 683 1077 1697 408
Size: 275 394 620 2913
Fragments arranged by size:
2913 620 394 275
XmaI C'CCGG_G
Cuts at: 1222 1222
Size: 4202
XmnI GAAnn'nnTTC
Cuts at: 307 307
Size: 4202
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI