Restriction map for
pF1KB7403
(Circular) (Six-base) MAPSORT of: pF1KB7403.seq Check: 476 from: 1 to: 4583
pF1KB7403 4583 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 917 1294 1621 917
Size: 377 327 3879
Fragments arranged by size:
3879 377 327
Acc65I G'GTAC_C
Cuts at: 1599 1599
Size: 4583
AclI AA'CG_TT
Cuts at: 4423 4423
Size: 4583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1232 2408 2840 3587 3855 553
Size: 679 1176 432 747 268 1281
Fragments arranged by size:
1281 1176 747 679 432 268
AflIII A'CryG_T
Cuts at: 1935 3039 4016 1935
Size: 1104 977 2502
Fragments arranged by size:
2502 1104 977
AgeI A'CCGG_T
Cuts at: 2971 2971
Size: 4583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1148 1148
Size: 4583
AlwNI CAG_nnn'CTG
Cuts at: 1070 1888 3455 1070
Size: 818 1567 2198
Fragments arranged by size:
2198 1567 818
ApaLI G'TGCA_C
Cuts at: 3353 3353
Size: 4583
ApoI r'AATT_y
Cuts at: 310 663 714 854 1276 1587 3936 4192 310
Size: 353 51 140 422 311 2349 256 701
Fragments arranged by size:
2349 701 422 353 311 256 140 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 4583
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 1603 1603
Size: 4583
BamHI G'GATC_C
Cuts at: 685 1608 685
Size: 923 3660
BanI G'GyrC_C
Cuts at: 961 1599 2013 2261 2296 961
Size: 638 414 248 35 3248
Fragments arranged by size:
3248 638 414 248 35
BanII G_rGCy'C
Cuts at: 1597 2627 1597
Size: 1030 3553
BbeI G_GCGC'C
Cuts at: 2017 2265 2017
Size: 248 4335
BbsI GAAGACnn'nnnn_
Cuts at: 922 922
Size: 4583
BciVI GTATCCnnnnn_n'
Cuts at: 2481 3242 4583 2481
Size: 761 1341 2481
Fragments arranged by size:
2481 1341 761
BclI T'GATC_A
Cuts at: 2103 2103
Size: 4583
BglII A'GATC_T
Cuts at: 2098 4135 2098
Size: 2037 2546
BlpI GC'TnA_GC
Cuts at: 1695 1695
Size: 4583
Bme1580I G_kGCm'C
Cuts at: 567 2208 2301 3357 567
Size: 1641 93 1056 1793
Fragments arranged by size:
1793 1641 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1795 1876 2211 4355 1795
Size: 81 335 2144 2023
Fragments arranged by size:
2144 2023 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 4583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 762 1290 762
Size: 528 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1759 2638 3130 3428 1759
Size: 879 492 298 2914
Fragments arranged by size:
2914 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4287 4287
Size: 4583
BsaAI yAC'GTr
Cuts at: 1936 1936
Size: 4583
BsaBI GATnn'nnATC
Cuts at: 6 690 2097 4134 6
Size: 684 1407 2037 455
Fragments arranged by size:
2037 1407 684 455
BsaHI Gr'CG_yC
Cuts at: 2014 2262 2964 4460 2014
Size: 248 702 1496 2137
Fragments arranged by size:
2137 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1772 2293 2971 3245 3392 3803 1772
Size: 521 678 274 147 411 2552
Fragments arranged by size:
2552 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1208 1238 1208
Size: 30 4553
BseYI C'CCAG_C
Cuts at: 1499 2008 2766 3343 3839 1499
Size: 509 758 577 496 2243
Fragments arranged by size:
2243 758 577 509 496
BsiEI CG_ry'CG
Cuts at: 104 2125 2171 3379 104
Size: 2021 46 1208 1308
Fragments arranged by size:
2021 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1597 2375 2565 3357 1597
Size: 778 190 792 2823
Fragments arranged by size:
2823 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 567 1597 2208 2301 2375 2565 2627 3357 567
Size: 1030 611 93 74 190 62 730 1793
Fragments arranged by size:
1793 1030 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3803 3803
Size: 4583
BspHI T'CATG_A
Cuts at: 342 3759 342
Size: 3417 1166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 588 916 1116 1633 1847 2149 2530 588
Size: 328 200 517 214 302 381 2641
Fragments arranged by size:
2641 517 381 328 302 214 200
BsrBI CCG'CTC
Cuts at: 50 2875 2929 4413 4578 50
Size: 2825 54 1484 165 55
Fragments arranged by size:
2825 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1263 2495 1263
Size: 1232 3351
BsrFI r'CCGG_y
Cuts at: 593 2581 2762 2971 593
Size: 1988 181 209 2205
Fragments arranged by size:
2205 1988 209 181
BssSI C'ACGA_G
Cuts at: 2854 3212 2854
Size: 358 4225
BstAPI GCAn_nnn'nTGC
Cuts at: 1960 1960
Size: 4583
BstBI TT'CG_AA
Cuts at: 2944 2944
Size: 4583
BstXI CCAn_nnnn'nTGG
Cuts at: 681 681
Size: 4583
BstYI r'GATC_y
Cuts at: 1 685 1608 2075 2098 2433 2679 3691 3777
Size: 684 923 467 23 335 246 1012 86
Cuts at: 3777 3789 4135 1
Size: 12 346 449
Fragments arranged by size:
1012 923 684 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 918 918
Size: 4583
Bsu36I CC'TnA_GG
Cuts at: 581 581
Size: 4583
BtgI C'CryG_G
Cuts at: 107 107
Size: 4583
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2533 2714 2877 2533
Size: 181 163 4239
Fragments arranged by size:
4239 181 163
BtsI GCAGTG_nn'
Cuts at: 1826 1826
Size: 4583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 971 1006 971
Size: 35 4548
DraI TTT'AAA
Cuts at: 1265 1582 1265
Size: 317 4266
DrdI GACnn_nn'nnGTC
Cuts at: 2289 3147 2289
Size: 858 3725
EaeI y'GGCC_r
Cuts at: 773 2168 2342 2733 2760 4105 773
Size: 1395 174 391 27 1345 1251
Fragments arranged by size:
1395 1345 1251 391 174 27
EagI C'GGCC_G
Cuts at: 2168 2168
Size: 4583
EarI CTCTTCn'nnn_
Cuts at: 1364 2606 2816 1364
Size: 1242 210 3131
Fragments arranged by size:
3131 1242 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2791 3101 3247 4393 2791
Size: 310 146 1146 2981
Fragments arranged by size:
2981 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1595 1595
Size: 4583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 762 1232 1290 2408 2840 3587 3855 553
Size: 209 470 58 1118 432 747 268 1281
Fragments arranged by size:
1281 1118 747 470 432 268 209 58
EcoO109I rG'GnC_Cy
Cuts at: 414 577 1722 414
Size: 163 1145 3275
Fragments arranged by size:
3275 1145 163
EcoRI G'AATT_C
Cuts at: 1276 1587 1276
Size: 311 4272
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4583
FspI TGC'GCA
Cuts at: 2364 2364
Size: 4583
HaeII r_GCGC'y
Cuts at: 2017 2265 3287 4266 2017
Size: 248 1022 979 2334
Fragments arranged by size:
2334 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 670 702 2425 2455 2457 2487 670
Size: 32 1723 30 2 30 2766
Fragments arranged by size:
2766 1723 32 30 30 2
HincII GTy'rAC
Cuts at: 1622 1622
Size: 4583
HindIII A'AGCT_T
Cuts at: 768 768
Size: 4583
KasI G'GCGC_C
Cuts at: 2013 2261 2013
Size: 248 4335
KpnI G_GTAC'C
Cuts at: 1603 1603
Size: 4583
MfeI C'AATT_G
Cuts at: 1427 1427
Size: 4583
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 971 3254 3438 971
Size: 2283 184 2116
Fragments arranged by size:
2283 2116 184
MscI TGG'CCA
Cuts at: 775 2344 775
Size: 1569 3014
MslI CAynn'nnrTG
Cuts at: 347 821 1573 2572 2699 347
Size: 474 752 999 127 2231
Fragments arranged by size:
2231 999 752 474 127
MspA1I CmG'CkG
Cuts at: 940 1694 2008 2368 3381 3626 940
Size: 754 314 360 1013 245 1897
Fragments arranged by size:
1897 1013 754 360 314 245
NaeI GCC'GGC
Cuts at: 595 2764 595
Size: 2169 2414
NarI GG'CG_CC
Cuts at: 2014 2262 2014
Size: 248 4335
NcoI C'CATG_G
Cuts at: 107 107
Size: 4583
NdeI CA'TA_TG
Cuts at: 822 822
Size: 4583
NgoMIV G'CCGG_C
Cuts at: 593 2762 593
Size: 2169 2414
NspI r_CATG'y
Cuts at: 1636 2136 3043 1636
Size: 500 907 3176
Fragments arranged by size:
3176 907 500
PciI A'CATG_T
Cuts at: 3039 3039
Size: 4583
PflMI CCAn_nnn'nTGG
Cuts at: 1518 1518
Size: 4583
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1530 1562 3722 3754 1530
Size: 32 2160 32 2359
Fragments arranged by size:
2359 2160 32 32
PpuMI rG'GwC_Cy
Cuts at: 414 577 414
Size: 163 4420
PstI C_TGCA'G
Cuts at: 1630 2315 1630
Size: 685 3898
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 940 2008 2368 940
Size: 1068 360 3155
Fragments arranged by size:
3155 1068 360
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 1597 1597
Size: 4583
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 2606 2816 2606
Size: 210 4373
SbfI CC_TGCA'GG
Cuts at: 1630 1630
Size: 4583
ScaI AGT'ACT
Cuts at: 1326 1375 4100 1326
Size: 49 2725 1809
Fragments arranged by size:
2725 1809 49
SexAI A'CCwGG_T
Cuts at: 997 997
Size: 4583
SfcI C'TryA_G
Cuts at: 33 203 860 887 1062 1377 1626 2311 3304
Size: 170 657 27 175 315 249 685 993
Cuts at: 3304 3495 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 657 315 249 191 175
170 27
SfoI GGC'GCC
Cuts at: 2015 2263 2015
Size: 248 4335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SmaI CCC'GGG
Cuts at: 1605 1605
Size: 4583
SmlI C'TyrA_G
Cuts at: 1738 2653 3145 3407 1738
Size: 915 492 262 2914
Fragments arranged by size:
2914 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 1636 2136 1636
Size: 500 4083
SspI AAT'ATT
Cuts at: 374 502 621 1140 374
Size: 128 119 519 3817
Fragments arranged by size:
3817 519 128 119
StyI C'CwwG_G
Cuts at: 107 985 1209 1717 2071 107
Size: 878 224 508 354 2619
Fragments arranged by size:
2619 878 508 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2166 2299 2758 2967 2166
Size: 133 459 209 3782
Fragments arranged by size:
3782 459 209 133
TatI w'GTAC_w
Cuts at: 1324 1373 4098 1324
Size: 49 2725 1809
Fragments arranged by size:
2725 1809 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 4551
Tth111I GACn'n_nGTC
Cuts at: 2380 2380
Size: 4583
XbaI T'CTAG_A
Cuts at: 1614 1614
Size: 4583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2078 2078
Size: 4583
XmaI C'CCGG_G
Cuts at: 1603 1603
Size: 4583
XmnI GAAnn'nnTTC
Cuts at: 314 314
Size: 4583
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII ClaI DraIII EcoNI EcoRV FseI FspAI
HpaI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI