Restriction map for
pF1KB7405
(Circular) (Six-base) MAPSORT of: pF1KB7405.seq Check: 7557 from: 1 to: 4568
pF1KB7405 4568 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 736 736
Size: 4568
AccI GT'mk_AC
Cuts at: 1606 1606
Size: 4568
Acc65I G'GTAC_C
Cuts at: 1584 1584
Size: 4568
AclI AA'CG_TT
Cuts at: 4408 4408
Size: 4568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 827 928 2393 2825 3572 3840 294
Size: 533 101 1465 432 747 268 1022
Fragments arranged by size:
1465 1022 747 533 432 268 101
AflIII A'CryG_T
Cuts at: 1920 3024 4001 1920
Size: 1104 977 2487
Fragments arranged by size:
2487 1104 977
AgeI A'CCGG_T
Cuts at: 2956 2956
Size: 4568
AleI CACnn'nnGTG
Cuts at: 1403 1403
Size: 4568
AlwNI CAG_nnn'CTG
Cuts at: 579 1873 3440 579
Size: 1294 1567 1707
Fragments arranged by size:
1707 1567 1294
ApaI G_GGCC'C
Cuts at: 1412 1412
Size: 4568
ApaLI G'TGCA_C
Cuts at: 1111 3338 1111
Size: 2227 2341
ApoI r'AATT_y
Cuts at: 1202 1572 3921 4177 1202
Size: 370 2349 256 1593
Fragments arranged by size:
2349 1593 370 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4568
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
AvaI C'yCGr_G
Cuts at: 302 427 445 1357 1588 302
Size: 125 18 912 231 3282
Fragments arranged by size:
3282 912 231 125 18
BamHI G'GATC_C
Cuts at: 1593 1593
Size: 4568
BanI G'GyrC_C
Cuts at: 293 1584 1998 2246 2281 293
Size: 1291 414 248 35 2580
Fragments arranged by size:
2580 1291 414 248 35
BanII G_rGCy'C
Cuts at: 1412 1582 2612 1412
Size: 170 1030 3368
Fragments arranged by size:
3368 1030 170
BbeI G_GCGC'C
Cuts at: 297 2002 2250 297
Size: 1705 248 2615
Fragments arranged by size:
2615 1705 248
BbsI GAAGACnn'nnnn_
Cuts at: 360 785 360
Size: 425 4143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 192 226 192
Size: 34 4534
BciVI GTATCCnnnnn_n'
Cuts at: 1175 2466 3227 4568 1175
Size: 1291 761 1341 1175
Fragments arranged by size:
1341 1291 1175 761
BclI T'GATC_A
Cuts at: 2088 2088
Size: 4568
BglII A'GATC_T
Cuts at: 2083 4120 2083
Size: 2037 2531
BlpI GC'TnA_GC
Cuts at: 355 1337 1394 1680 355
Size: 982 57 286 3243
Fragments arranged by size:
3243 982 286 57
Bme1580I G_kGCm'C
Cuts at: 1115 1365 1412 2193 2286 3342 1115
Size: 250 47 781 93 1056 2341
Fragments arranged by size:
2341 1056 781 250 93 47
BmgBI CAC'GTC
Cuts at: 131 1380 131
Size: 1249 3319
BmrI ACTGGGnnnn_n'
Cuts at: 1780 1861 2196 4340 1780
Size: 81 335 2144 2008
Fragments arranged by size:
2144 2008 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 204 2935 204
Size: 2731 1837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 1300
Size: 4568
Bpu10I CC'TnA_GC
Cuts at: 271 271
Size: 4568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1744 2623 3115 3413 1744
Size: 879 492 298 2899
Fragments arranged by size:
2899 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 192 1003 4272 192
Size: 811 3269 488
Fragments arranged by size:
3269 811 488
BsaAI yAC'GTr
Cuts at: 1921 1921
Size: 4568
BsaBI GATnn'nnATC
Cuts at: 6 2082 4119 6
Size: 2076 2037 455
Fragments arranged by size:
2076 2037 455
BsaHI Gr'CG_yC
Cuts at: 294 467 1999 2247 2949 4445 294
Size: 173 1532 248 702 1496 417
Fragments arranged by size:
1532 1496 702 417 248 173
BsaWI w'CCGG_w
Cuts at: 1757 2278 2956 3230 3377 3788 1757
Size: 521 678 274 147 411 2537
Fragments arranged by size:
2537 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 226 256 226
Size: 30 4538
BseYI C'CCAG_C
Cuts at: 949 1993 2751 3328 3824 949
Size: 1044 758 577 496 1693
Fragments arranged by size:
1693 1044 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 1138
Size: 4568
BsiEI CG_ry'CG
Cuts at: 104 677 728 2110 2156 3364 104
Size: 573 51 1382 46 1208 1308
Fragments arranged by size:
1382 1308 1208 573 51 46
BsiHKAI G_wGCw'C
Cuts at: 158 458 1115 1400 1582 2360 2550 3342 158
Size: 300 657 285 182 778 190 792 1384
Fragments arranged by size:
1384 792 778 657 300 285 190 182
BsmBI CGTCTCn'nnnn_
Cuts at: 1445 1445
Size: 4568
Bsp1286I G_dGCh'C
Cuts at: 158 458 1115 1365 1400 1412 1582 2193 2286
Size: 300 657 250 35 12 170 611 93
Cuts at: 2286 2360 2550 2612 3342 158
Size: 74 190 62 730 1384
Fragments arranged by size:
1384 730 657 611 300 250 190 170
93 74 62 35 12
BspEI T'CCGG_A
Cuts at: 3788 3788
Size: 4568
BspHI T'CATG_A
Cuts at: 3744 3744
Size: 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 736 1618 1832 2134 2515 736
Size: 882 214 302 381 2789
Fragments arranged by size:
2789 882 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2860 2914 4398 4563 50
Size: 2810 54 1484 165 55
Fragments arranged by size:
2810 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2480 2480
Size: 4568
BsrFI r'CCGG_y
Cuts at: 134 277 2566 2747 2956 134
Size: 143 2289 181 209 1746
Fragments arranged by size:
2289 1746 209 181 143
BsrGI T'GTAC_A
Cuts at: 1101 1101
Size: 4568
BssHII G'CGCG_C
Cuts at: 350 639 350
Size: 289 4279
BssSI C'ACGA_G
Cuts at: 2839 3197 2839
Size: 358 4210
BstAPI GCAn_nnn'nTGC
Cuts at: 131 1945 131
Size: 1814 2754
BstBI TT'CG_AA
Cuts at: 2929 2929
Size: 4568
BstXI CCAn_nnnn'nTGG
Cuts at: 399 399
Size: 4568
BstYI r'GATC_y
Cuts at: 1 1593 2060 2083 2418 2664 3676 3762 3774
Size: 1592 467 23 335 246 1012 86 12
Cuts at: 3774 4120 1
Size: 346 449
Fragments arranged by size:
1592 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 617 1169 617
Size: 552 4016
BtgI C'CryG_G
Cuts at: 107 634 863 1503 107
Size: 527 229 640 3172
Fragments arranged by size:
3172 640 527 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 195 270 2518 2699 2862 195
Size: 75 2248 181 163 1901
Fragments arranged by size:
2248 1901 181 163 75
BtsI GCAGTG_nn'
Cuts at: 940 1104 1363 1811 940
Size: 164 259 448 3697
Fragments arranged by size:
3697 448 259 164
ClaI AT'CG_AT
Cuts at: 437 437
Size: 4568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1445 1480 1445
Size: 35 4533
DraI TTT'AAA
Cuts at: 1567 1567
Size: 4568
DraIII CAC_nnn'GTG
Cuts at: 1239 1239
Size: 4568
DrdI GACnn_nn'nnGTC
Cuts at: 2274 3132 2274
Size: 858 3710
EaeI y'GGCC_r
Cuts at: 674 881 2153 2327 2718 2745 4090 674
Size: 207 1272 174 391 27 1345 1152
Fragments arranged by size:
1345 1272 1152 391 207 174 27
EagI C'GGCC_G
Cuts at: 674 2153 674
Size: 1479 3089
EarI CTCTTCn'nnn_
Cuts at: 148 904 1052 1443 2591 2801 148
Size: 756 148 391 1148 210 1915
Fragments arranged by size:
1915 1148 756 391 210 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 193 598 661 2776 3086 3232 4378 193
Size: 405 63 2115 310 146 1146 383
Fragments arranged by size:
2115 1146 405 383 310 146 63
EcoICRI GAG'CTC
Cuts at: 1580 1580
Size: 4568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 827 928 1300 2393 2825 3572 3840 294
Size: 533 101 372 1093 432 747 268 1022
Fragments arranged by size:
1093 1022 747 533 432 372 268 101
EcoO109I rG'GnC_Cy
Cuts at: 613 630 692 918 1409 1707 613
Size: 17 62 226 491 298 3474
Fragments arranged by size:
3474 491 298 226 62 17
EcoRI G'AATT_C
Cuts at: 1572 1572
Size: 4568
EcoRV GAT'ATC
Cuts at: 1183 1183
Size: 4568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2587 2587
Size: 4568
FspI TGC'GCA
Cuts at: 2349 2349
Size: 4568
HaeII r_GCGC'y
Cuts at: 278 297 317 605 2002 2250 3272 4251 278
Size: 19 20 288 1397 248 1022 979 595
Fragments arranged by size:
1397 1022 979 595 288 248 20 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 787 819 2410 2440 2442 2472 238
Size: 32 517 32 1591 30 2 30 2334
Fragments arranged by size:
2334 1591 517 32 32 30 30 2
HincII GTy'rAC
Cuts at: 486 1050 1548 1607 486
Size: 564 498 59 3447
Fragments arranged by size:
3447 564 498 59
KasI G'GCGC_C
Cuts at: 293 1998 2246 293
Size: 1705 248 2615
Fragments arranged by size:
2615 1705 248
KpnI G_GTAC'C
Cuts at: 1588 1588
Size: 4568
MluI A'CGCG_T
Cuts at: 4001 4001
Size: 4568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 269 3239 3423 248
Size: 21 2970 184 1393
Fragments arranged by size:
2970 1393 184 21
MscI TGG'CCA
Cuts at: 2329 2329
Size: 4568
MslI CAynn'nnrTG
Cuts at: 151 397 1382 1403 2557 2684 151
Size: 246 985 21 1154 127 2035
Fragments arranged by size:
2035 1154 985 246 127 21
MspA1I CmG'CkG
Cuts at: 570 579 636 1015 1344 1679 1993 2353 3366
Size: 9 57 379 329 335 314 360 1013
Cuts at: 3366 3611 570
Size: 245 1527
Fragments arranged by size:
1527 1013 379 360 335 329 314 245
57 9
NaeI GCC'GGC
Cuts at: 136 279 2749 136
Size: 143 2470 1955
Fragments arranged by size:
2470 1955 143
NarI GG'CG_CC
Cuts at: 294 1999 2247 294
Size: 1705 248 2615
Fragments arranged by size:
2615 1705 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4568
NgoMIV G'CCGG_C
Cuts at: 134 277 2747 134
Size: 143 2470 1955
Fragments arranged by size:
2470 1955 143
NspI r_CATG'y
Cuts at: 240 841 1621 2121 3028 240
Size: 601 780 500 907 1780
Fragments arranged by size:
1780 907 780 601 500
PasI CC'CwG_GG
Cuts at: 855 855
Size: 4568
PciI A'CATG_T
Cuts at: 3024 3024
Size: 4568
PfoI T'CCnGG_A
Cuts at: 509 509
Size: 4568
PmeI GTTT'AAAC
Cuts at: 1567 1567
Size: 4568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4536
PpuMI rG'GwC_Cy
Cuts at: 630 692 918 630
Size: 62 226 4280
Fragments arranged by size:
4280 226 62
PspOMI G'GGCC_C
Cuts at: 1408 1408
Size: 4568
PstI C_TGCA'G
Cuts at: 566 1615 2300 566
Size: 1049 685 2834
Fragments arranged by size:
2834 1049 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4568
PvuII CAG'CTG
Cuts at: 579 1344 1993 2353 579
Size: 765 649 360 2794
Fragments arranged by size:
2794 765 649 360
RsrII CG'GwC_CG
Cuts at: 2763 2763
Size: 4568
SacI G_AGCT'C
Cuts at: 1582 1582
Size: 4568
SacII CC_GC'GG
Cuts at: 637 637
Size: 4568
SalI G'TCGA_C
Cuts at: 1605 1605
Size: 4568
SapI GCTCTTCn'nnn_
Cuts at: 148 1052 2591 2801 148
Size: 904 1539 210 1915
Fragments arranged by size:
1915 1539 904 210
SbfI CC_TGCA'GG
Cuts at: 1615 1615
Size: 4568
ScaI AGT'ACT
Cuts at: 4085 4085
Size: 4568
SfcI C'TryA_G
Cuts at: 33 562 1027 1611 2296 3289 3480 33
Size: 529 465 584 685 993 191 1121
Fragments arranged by size:
1121 993 685 584 529 465 191
SfoI GGC'GCC
Cuts at: 295 2000 2248 295
Size: 1705 248 2615
Fragments arranged by size:
2615 1705 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
SmaI CCC'GGG
Cuts at: 304 1359 1590 304
Size: 1055 231 3282
Fragments arranged by size:
3282 1055 231
SmlI C'TyrA_G
Cuts at: 1723 2638 3130 3392 1723
Size: 915 492 262 2899
Fragments arranged by size:
2899 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4568
SphI G_CATG'C
Cuts at: 1621 2121 1621
Size: 500 4068
StyI C'CwwG_G
Cuts at: 107 1325 1702 2056 107
Size: 1218 377 354 2619
Fragments arranged by size:
2619 1218 377 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 778 2151 2284 2743 2952 778
Size: 1373 133 459 209 2394
Fragments arranged by size:
2394 1373 459 209 133
TatI w'GTAC_w
Cuts at: 828 1101 4083 828
Size: 273 2982 1313
Fragments arranged by size:
2982 1313 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4085 4117 4085
Size: 32 4536
Tth111I GACn'n_nGTC
Cuts at: 551 1511 2365 551
Size: 960 854 2754
Fragments arranged by size:
2754 960 854
XbaI T'CTAG_A
Cuts at: 1416 1599 1416
Size: 183 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2063 2063
Size: 4568
XmaI C'CCGG_G
Cuts at: 302 1357 1588 302
Size: 1055 231 3282
Fragments arranged by size:
3282 1055 231
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BseRI BsiWI BsmI
BstEII BstZ17I EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI