Restriction map for
pF1KB7406
(Circular) (Six-base) MAPSORT of: pF1KB7406.seq Check: 336 from: 1 to: 3770
pF1KB7406 3770 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 682 808 682
Size: 126 3644
Acc65I G'GTAC_C
Cuts at: 786 786
Size: 3770
AclI AA'CG_TT
Cuts at: 3610 3610
Size: 3770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1595 2027 2774 3042 1595
Size: 432 747 268 2323
Fragments arranged by size:
2323 747 432 268
AflIII A'CryG_T
Cuts at: 191 1122 2226 3203 191
Size: 931 1104 977 758
Fragments arranged by size:
1104 977 931 758
AgeI A'CCGG_T
Cuts at: 2158 2158
Size: 3770
AlwNI CAG_nnn'CTG
Cuts at: 1075 2642 1075
Size: 1567 2203
ApaLI G'TGCA_C
Cuts at: 2540 2540
Size: 3770
ApoI r'AATT_y
Cuts at: 774 3123 3379 774
Size: 2349 256 1165
Fragments arranged by size:
2349 1165 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
AvaI C'yCGr_G
Cuts at: 258 790 258
Size: 532 3238
BamHI G'GATC_C
Cuts at: 795 795
Size: 3770
BanI G'GyrC_C
Cuts at: 163 786 1200 1448 1483 163
Size: 623 414 248 35 2450
Fragments arranged by size:
2450 623 414 248 35
BanII G_rGCy'C
Cuts at: 784 1814 784
Size: 1030 2740
BbeI G_GCGC'C
Cuts at: 1204 1452 1204
Size: 248 3522
BciVI GTATCCnnnnn_n'
Cuts at: 1668 2429 3770 1668
Size: 761 1341 1668
Fragments arranged by size:
1668 1341 761
BclI T'GATC_A
Cuts at: 1290 1290
Size: 3770
BglII A'GATC_T
Cuts at: 1285 3322 1285
Size: 2037 1733
BlpI GC'TnA_GC
Cuts at: 882 882
Size: 3770
Bme1580I G_kGCm'C
Cuts at: 166 1395 1488 2544 166
Size: 1229 93 1056 1392
Fragments arranged by size:
1392 1229 1056 93
BmgBI CAC'GTC
Cuts at: 192 192
Size: 3770
BmrI ACTGGGnnnn_n'
Cuts at: 982 1063 1398 3542 982
Size: 81 335 2144 1210
Fragments arranged by size:
2144 1210 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 3770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 271 319 205
Size: 66 48 3656
Fragments arranged by size:
3656 66 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 946 1825 2317 2615 229
Size: 717 879 492 298 1384
Fragments arranged by size:
1384 879 717 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3474 3474
Size: 3770
BsaAI yAC'GTr
Cuts at: 1123 1123
Size: 3770
BsaBI GATnn'nnATC
Cuts at: 6 1284 3321 6
Size: 1278 2037 455
Fragments arranged by size:
2037 1278 455
BsaHI Gr'CG_yC
Cuts at: 125 1201 1449 2151 3647 125
Size: 1076 248 702 1496 248
Fragments arranged by size:
1496 1076 702 248 248
BsaWI w'CCGG_w
Cuts at: 959 1480 2158 2432 2579 2990 959
Size: 521 678 274 147 411 1739
Fragments arranged by size:
1739 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 178 208 178
Size: 30 3740
BseYI C'CCAG_C
Cuts at: 221 409 1195 1953 2530 3026 221
Size: 188 786 758 577 496 965
Fragments arranged by size:
965 786 758 577 496 188
BsiEI CG_ry'CG
Cuts at: 104 1312 1358 2566 104
Size: 1208 46 1208 1308
Fragments arranged by size:
1308 1208 1208 46
BsiHKAI G_wGCw'C
Cuts at: 784 1562 1752 2544 784
Size: 778 190 792 2010
Fragments arranged by size:
2010 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 166 784 1395 1488 1562 1752 1814 2544 166
Size: 618 611 93 74 190 62 730 1392
Fragments arranged by size:
1392 730 618 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2990 2990
Size: 3770
BspHI T'CATG_A
Cuts at: 2946 2946
Size: 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 820 1034 1336 1717 820
Size: 214 302 381 2873
Fragments arranged by size:
2873 381 302 214
BsrBI CCG'CTC
Cuts at: 50 303 336 2062 2116 3600 3765 50
Size: 253 33 1726 54 1484 165 55
Fragments arranged by size:
1726 1484 253 165 55 54 33
BsrDI GCAATG_nn'
Cuts at: 1682 1682
Size: 3770
BsrFI r'CCGG_y
Cuts at: 239 1768 1949 2158 239
Size: 1529 181 209 1851
Fragments arranged by size:
1851 1529 209 181
BssSI C'ACGA_G
Cuts at: 2041 2399 2041
Size: 358 3412
BstAPI GCAn_nnn'nTGC
Cuts at: 1147 1147
Size: 3770
BstBI TT'CG_AA
Cuts at: 2131 2131
Size: 3770
BstXI CCAn_nnnn'nTGG
Cuts at: 714 714
Size: 3770
BstYI r'GATC_y
Cuts at: 1 132 795 1262 1285 1620 1866 2878 2964
Size: 131 663 467 23 335 246 1012 86
Cuts at: 2964 2976 3322 1
Size: 12 346 449
Fragments arranged by size:
1012 663 467 449 346 335 246 131
86 23 12
BtgI C'CryG_G
Cuts at: 107 354 107
Size: 247 3523
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1720 1901 2064 1720
Size: 181 163 3426
Fragments arranged by size:
3426 181 163
BtsI GCAGTG_nn'
Cuts at: 648 1013 648
Size: 365 3405
DraI TTT'AAA
Cuts at: 769 769
Size: 3770
DraIII CAC_nnn'GTG
Cuts at: 675 675
Size: 3770
DrdI GACnn_nn'nnGTC
Cuts at: 1476 2334 1476
Size: 858 2912
EaeI y'GGCC_r
Cuts at: 227 314 1355 1529 1920 1947 3292 227
Size: 87 1041 174 391 27 1345 705
Fragments arranged by size:
1345 1041 705 391 174 87 27
EagI C'GGCC_G
Cuts at: 1355 1355
Size: 3770
EarI CTCTTCn'nnn_
Cuts at: 608 1793 2003 608
Size: 1185 210 2375
Fragments arranged by size:
2375 1185 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1978 2288 2434 3580 1978
Size: 310 146 1146 2168
Fragments arranged by size:
2168 1146 310 146
EcoICRI GAG'CTC
Cuts at: 782 782
Size: 3770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 271 319 1595 2027 2774 3042 205
Size: 66 48 1276 432 747 268 933
Fragments arranged by size:
1276 933 747 432 268 66 48
EcoO109I rG'GnC_Cy
Cuts at: 622 909 622
Size: 287 3483
EcoRI G'AATT_C
Cuts at: 774 774
Size: 3770
EcoRV GAT'ATC
Cuts at: 423 423
Size: 3770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1789 1789
Size: 3770
FspI TGC'GCA
Cuts at: 1551 1551
Size: 3770
HaeII r_GCGC'y
Cuts at: 1204 1452 2474 3453 1204
Size: 248 1022 979 1521
Fragments arranged by size:
1521 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 330 362 1612 1642 1644 1674 330
Size: 32 1250 30 2 30 2426
Fragments arranged by size:
2426 1250 32 30 30 2
HincII GTy'rAC
Cuts at: 809 809
Size: 3770
KasI G'GCGC_C
Cuts at: 1200 1448 1200
Size: 248 3522
KpnI G_GTAC'C
Cuts at: 790 790
Size: 3770
MluI A'CGCG_T
Cuts at: 3203 3203
Size: 3770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2441 2625 2441
Size: 184 3586
MscI TGG'CCA
Cuts at: 316 1531 316
Size: 1215 2555
MslI CAynn'nnrTG
Cuts at: 1759 1886 1759
Size: 127 3643
MspA1I CmG'CkG
Cuts at: 216 225 232 561 590 881 1195 1555 2568
Size: 9 7 329 29 291 314 360 1013
Cuts at: 2568 2813 216
Size: 245 1173
Fragments arranged by size:
1173 1013 360 329 314 291 245 29
9 7
NaeI GCC'GGC
Cuts at: 241 1951 241
Size: 1710 2060
NarI GG'CG_CC
Cuts at: 1201 1449 1201
Size: 248 3522
NcoI C'CATG_G
Cuts at: 107 107
Size: 3770
NgoMIV G'CCGG_C
Cuts at: 239 1949 239
Size: 1710 2060
NspI r_CATG'y
Cuts at: 823 1323 2230 823
Size: 500 907 2363
Fragments arranged by size:
2363 907 500
PciI A'CATG_T
Cuts at: 2226 2226
Size: 3770
PmeI GTTT'AAAC
Cuts at: 769 769
Size: 3770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 3738
PshAI GACnn'nnGTC
Cuts at: 561 561
Size: 3770
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 513 545 513
Size: 32 3738
PstI C_TGCA'G
Cuts at: 817 1502 817
Size: 685 3085
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3770
PvuII CAG'CTG
Cuts at: 225 561 590 1195 1555 225
Size: 336 29 605 360 2440
Fragments arranged by size:
2440 605 360 336 29
RsrII CG'GwC_CG
Cuts at: 1965 1965
Size: 3770
SacI G_AGCT'C
Cuts at: 784 784
Size: 3770
SalI G'TCGA_C
Cuts at: 807 807
Size: 3770
SapI GCTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
SbfI CC_TGCA'GG
Cuts at: 817 817
Size: 3770
ScaI AGT'ACT
Cuts at: 3287 3287
Size: 3770
SfcI C'TryA_G
Cuts at: 33 516 813 1498 2491 2682 33
Size: 483 297 685 993 191 1121
Fragments arranged by size:
1121 993 685 483 297 191
SfoI GGC'GCC
Cuts at: 1202 1450 1202
Size: 248 3522
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
SmaI CCC'GGG
Cuts at: 792 792
Size: 3770
SmlI C'TyrA_G
Cuts at: 244 925 1840 2332 2594 244
Size: 681 915 492 262 1420
Fragments arranged by size:
1420 915 681 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3770
SphI G_CATG'C
Cuts at: 823 1323 823
Size: 500 3270
StyI C'CwwG_G
Cuts at: 107 323 625 904 1258 107
Size: 216 302 279 354 2619
Fragments arranged by size:
2619 354 302 279 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 592 1353 1486 1945 2154 592
Size: 761 133 459 209 2208
Fragments arranged by size:
2208 761 459 209 133
TatI w'GTAC_w
Cuts at: 3285 3285
Size: 3770
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 3738
Tth111I GACn'n_nGTC
Cuts at: 1567 1567
Size: 3770
XbaI T'CTAG_A
Cuts at: 801 801
Size: 3770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1265 1265
Size: 3770
XmaI C'CCGG_G
Cuts at: 790 790
Size: 3770
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PshAI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI