Restriction map for
pF1KB7407
(Circular) (Six-base) MAPSORT of: pF1KB7407.seq Check: 9349 from: 1 to: 3752
pF1KB7407 3752 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 224 790 224
Size: 566 3186
Acc65I G'GTAC_C
Cuts at: 768 768
Size: 3752
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1577 2009 2756 3024 625
Size: 952 432 747 268 1353
Fragments arranged by size:
1353 952 747 432 268
AfeI AGC'GCT
Cuts at: 439 439
Size: 3752
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 1057 2624 1057
Size: 1567 2185
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 466 687 772 466
Size: 221 85 3446
Fragments arranged by size:
3446 221 85
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 111 339 462 768 1182 1430 1465 111
Size: 228 123 306 414 248 35 2398
Fragments arranged by size:
2398 414 306 248 228 123 35
BanII G_rGCy'C
Cuts at: 656 766 1796 656
Size: 110 1030 2612
Fragments arranged by size:
2612 1030 110
BbeI G_GCGC'C
Cuts at: 115 343 466 1186 1434 115
Size: 228 123 720 248 2433
Fragments arranged by size:
2433 720 248 228 123
BbvCI CC'TCA_GC
Cuts at: 709 709
Size: 3752
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2411 3752 1650
Size: 761 1341 1650
Fragments arranged by size:
1650 1341 761
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglI GCCn_nnn'nGGC
Cuts at: 428 471 692 428
Size: 43 221 3488
Fragments arranged by size:
3488 221 43
BglII A'GATC_T
Cuts at: 1267 3304 1267
Size: 2037 1715
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 1377 1470 2526 1377
Size: 93 1056 2603
Fragments arranged by size:
2603 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 964 1045 1380 3524 964
Size: 81 335 2144 1192
Fragments arranged by size:
2144 1192 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
Bpu10I CC'TnA_GC
Cuts at: 650 709 650
Size: 59 3693
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 928 1807 2299 2597 463
Size: 465 879 492 298 1618
Fragments arranged by size:
1618 879 492 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 625 3456 625
Size: 2831 921
BsaAI yAC'GTr
Cuts at: 375 615 1105 375
Size: 240 490 3022
Fragments arranged by size:
3022 490 240
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 112 191 340 463 1183 1431 2133 3629 112
Size: 79 149 123 720 248 702 1496 235
Fragments arranged by size:
1496 720 702 248 235 149 123 79
BsaWI w'CCGG_w
Cuts at: 941 1462 2140 2414 2561 2972 941
Size: 521 678 274 147 411 1721
Fragments arranged by size:
1721 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 440 1177 1935 2512 3008 440
Size: 737 758 577 496 1184
Fragments arranged by size:
1184 758 737 577 496
BsiEI CG_ry'CG
Cuts at: 104 356 622 1294 1340 2548 104
Size: 252 266 672 46 1208 1308
Fragments arranged by size:
1308 1208 672 266 252 46
BsiHKAI G_wGCw'C
Cuts at: 371 728 766 1544 1734 2526 371
Size: 357 38 778 190 792 1597
Fragments arranged by size:
1597 792 778 357 190 38
Bsp1286I G_dGCh'C
Cuts at: 371 656 728 766 1377 1470 1544 1734 1796
Size: 285 72 38 611 93 74 190 62
Cuts at: 1796 2526 371
Size: 730 1597
Fragments arranged by size:
1597 730 611 285 190 93 74 72
62 38
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 3752
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1016 1318 1699 802
Size: 214 302 381 2855
Fragments arranged by size:
2855 381 302 214
BsrBI CCG'CTC
Cuts at: 50 477 2044 2098 3582 3747 50
Size: 427 1567 54 1484 165 55
Fragments arranged by size:
1567 1484 427 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 351 452 587 1750 1931 2140 351
Size: 101 135 1163 181 209 1963
Fragments arranged by size:
1963 1163 209 181 135 101
BsrGI T'GTAC_A
Cuts at: 380 380
Size: 3752
BssHII G'CGCG_C
Cuts at: 178 180 178
Size: 2 3750
BssSI C'ACGA_G
Cuts at: 544 675 2023 2381 544
Size: 131 1348 358 1915
Fragments arranged by size:
1915 1348 358 131
BstAPI GCAn_nnn'nTGC
Cuts at: 375 1129 375
Size: 754 2998
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstXI CCAn_nnnn'nTGG
Cuts at: 582 582
Size: 3752
BstYI r'GATC_y
Cuts at: 1 777 1244 1267 1602 1848 2860 2946 2958
Size: 776 467 23 335 246 1012 86 12
Cuts at: 2958 3304 1
Size: 346 449
Fragments arranged by size:
1012 776 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 458 107
Size: 351 3401
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1702 1883 2046 1702
Size: 181 163 3408
Fragments arranged by size:
3408 181 163
BtsI GCAGTG_nn'
Cuts at: 995 995
Size: 3752
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DraIII CAC_nnn'GTG
Cuts at: 375 375
Size: 3752
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 353 540 619 1337 1511 1902 1929 3274 353
Size: 187 79 718 174 391 27 1345 831
Fragments arranged by size:
1345 831 718 391 187 174 79 27
EagI C'GGCC_G
Cuts at: 353 619 1337 353
Size: 266 718 2768
Fragments arranged by size:
2768 718 266
EarI CTCTTCn'nnn_
Cuts at: 508 709 1775 1985 508
Size: 201 1066 210 2275
Fragments arranged by size:
2275 1066 210 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1960 2270 2416 3562 1960
Size: 310 146 1146 2150
Fragments arranged by size:
2150 1146 310 146
EcoICRI GAG'CTC
Cuts at: 764 764
Size: 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1577 2009 2756 3024 625
Size: 952 432 747 268 1353
Fragments arranged by size:
1353 952 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 640 640
Size: 3752
EcoO109I rG'GnC_Cy
Cuts at: 891 891
Size: 3752
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 115 126 343 441 466 1186 1434 2456 3435
Size: 11 217 98 25 720 248 1022 979
Cuts at: 3435 115
Size: 432
Fragments arranged by size:
1022 979 720 432 248 217 98 25
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1594 1624 1626 1656 1594
Size: 30 2 30 3690
Fragments arranged by size:
3690 30 30 2
HincII GTy'rAC
Cuts at: 390 791 390
Size: 401 3351
KasI G'GCGC_C
Cuts at: 111 339 462 1182 1430 111
Size: 228 123 720 248 2433
Fragments arranged by size:
2433 720 248 228 123
KpnI G_GTAC'C
Cuts at: 772 772
Size: 3752
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 721 1741 1868 721
Size: 1020 127 2605
Fragments arranged by size:
2605 1020 127
MspA1I CmG'CkG
Cuts at: 129 138 159 345 460 863 1177 1537 2550
Size: 9 21 186 115 403 314 360 1013
Cuts at: 2550 2795 129
Size: 245 1086
Fragments arranged by size:
1086 1013 403 360 314 245 186 115
21 9
NaeI GCC'GGC
Cuts at: 353 1933 353
Size: 1580 2172
NarI GG'CG_CC
Cuts at: 112 340 463 1183 1431 112
Size: 228 123 720 248 2433
Fragments arranged by size:
2433 720 248 228 123
NcoI C'CATG_G
Cuts at: 107 107
Size: 3752
NgoMIV G'CCGG_C
Cuts at: 351 1931 351
Size: 1580 2172
NspI r_CATG'y
Cuts at: 805 1305 2212 805
Size: 500 907 2345
Fragments arranged by size:
2345 907 500
PasI CC'CwG_GG
Cuts at: 519 519
Size: 3752
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PstI C_TGCA'G
Cuts at: 799 1484 799
Size: 685 3067
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 1177 1537 1177
Size: 360 3392
RsrII CG'GwC_CG
Cuts at: 210 1947 210
Size: 1737 2015
SacI G_AGCT'C
Cuts at: 766 766
Size: 3752
SacII CC_GC'GG
Cuts at: 461 461
Size: 3752
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 508 1775 1985 508
Size: 1267 210 2275
Fragments arranged by size:
2275 1267 210
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 795 1480 2473 2664 33
Size: 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 191
SfoI GGC'GCC
Cuts at: 113 341 464 1184 1432 113
Size: 228 123 720 248 2433
Fragments arranged by size:
2433 720 248 228 123
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 468 689 774 468
Size: 221 85 3446
Fragments arranged by size:
3446 221 85
SmlI C'TyrA_G
Cuts at: 478 907 1822 2314 2576 478
Size: 429 915 492 262 1654
Fragments arranged by size:
1654 915 492 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
SrfI GCCC'GGGC
Cuts at: 689 689
Size: 3752
StyI C'CwwG_G
Cuts at: 107 886 1240 107
Size: 779 354 2619
Fragments arranged by size:
2619 779 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1335 1468 1927 2136 1335
Size: 133 459 209 2951
Fragments arranged by size:
2951 459 209 133
TatI w'GTAC_w
Cuts at: 380 3267 380
Size: 2887 865
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 3720
Tth111I GACn'n_nGTC
Cuts at: 1549 1549
Size: 3752
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1247 1247
Size: 3752
XmaI C'CCGG_G
Cuts at: 466 687 772 466
Size: 221 85 3446
Fragments arranged by size:
3446 221 85
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI