Restriction map for
pF1KB7409
(Circular) (Six-base) MAPSORT of: pF1KB7409.seq Check: 329 from: 1 to: 3683
pF1KB7409 3683 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 454 721 454
Size: 267 3416
Acc65I G'GTAC_C
Cuts at: 699 699
Size: 3683
AclI AA'CG_TT
Cuts at: 3523 3523
Size: 3683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 1508 1940 2687 2955 497
Size: 1011 432 747 268 1225
Fragments arranged by size:
1225 1011 747 432 268
AflIII A'CryG_T
Cuts at: 1035 2139 3116 1035
Size: 1104 977 1602
Fragments arranged by size:
1602 1104 977
AgeI A'CCGG_T
Cuts at: 2071 2071
Size: 3683
AhdI GACnn_n'nnGTC
Cuts at: 450 450
Size: 3683
AlwNI CAG_nnn'CTG
Cuts at: 988 2555 988
Size: 1567 2116
ApaLI G'TGCA_C
Cuts at: 2453 2453
Size: 3683
ApoI r'AATT_y
Cuts at: 687 3036 3292 687
Size: 2349 256 1078
Fragments arranged by size:
2349 1078 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3683
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
AvaI C'yCGr_G
Cuts at: 166 703 166
Size: 537 3146
AvrII C'CTAG_G
Cuts at: 286 286
Size: 3683
BamHI G'GATC_C
Cuts at: 233 708 233
Size: 475 3208
BanI G'GyrC_C
Cuts at: 699 1113 1361 1396 699
Size: 414 248 35 2986
Fragments arranged by size:
2986 414 248 35
BanII G_rGCy'C
Cuts at: 116 135 214 697 1727 116
Size: 19 79 483 1030 2072
Fragments arranged by size:
2072 1030 483 79 19
BbeI G_GCGC'C
Cuts at: 1117 1365 1117
Size: 248 3435
BciVI GTATCCnnnnn_n'
Cuts at: 1581 2342 3683 1581
Size: 761 1341 1581
Fragments arranged by size:
1581 1341 761
BclI T'GATC_A
Cuts at: 1203 1203
Size: 3683
BglI GCCn_nnn'nGGC
Cuts at: 139 139
Size: 3683
BglII A'GATC_T
Cuts at: 1198 3235 1198
Size: 2037 1646
BlpI GC'TnA_GC
Cuts at: 254 795 254
Size: 541 3142
Bme1580I G_kGCm'C
Cuts at: 1308 1401 2457 1308
Size: 93 1056 2534
Fragments arranged by size:
2534 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 412 624 895 976 1311 3455 412
Size: 212 271 81 335 2144 640
Fragments arranged by size:
2144 640 335 271 212 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 3683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 266 201
Size: 65 3618
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 633 859 1738 2230 2528 620
Size: 13 226 879 492 298 1775
Fragments arranged by size:
1775 879 492 298 226 13
BsaI GGTCTCn'nnnn_
Cuts at: 560 3387 560
Size: 2827 856
BsaAI yAC'GTr
Cuts at: 1036 1036
Size: 3683
BsaBI GATnn'nnATC
Cuts at: 6 1197 3234 6
Size: 1191 2037 455
Fragments arranged by size:
2037 1191 455
BsaHI Gr'CG_yC
Cuts at: 1114 1362 2064 3560 1114
Size: 248 702 1496 1237
Fragments arranged by size:
1496 1237 702 248
BsaWI w'CCGG_w
Cuts at: 872 1393 2071 2345 2492 2903 872
Size: 521 678 274 147 411 1652
Fragments arranged by size:
1652 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 636 657 636
Size: 21 3662
BseYI C'CCAG_C
Cuts at: 1108 1866 2443 2939 1108
Size: 758 577 496 1852
Fragments arranged by size:
1852 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 128 1225 1271 2479 104
Size: 24 1097 46 1208 1308
Fragments arranged by size:
1308 1208 1097 46 24
BsiHKAI G_wGCw'C
Cuts at: 667 697 1475 1665 2457 667
Size: 30 778 190 792 1893
Fragments arranged by size:
1893 792 778 190 30
Bsp1286I G_dGCh'C
Cuts at: 116 135 214 667 697 1308 1401 1475 1665
Size: 19 79 453 30 611 93 74 190
Cuts at: 1665 1727 2457 116
Size: 62 730 1342
Fragments arranged by size:
1342 730 611 453 190 93 79 74
62 30 19
BspEI T'CCGG_A
Cuts at: 2903 2903
Size: 3683
BspHI T'CATG_A
Cuts at: 2859 2859
Size: 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 389 733 947 1249 1630 389
Size: 344 214 302 381 2442
Fragments arranged by size:
2442 381 344 302 214
BsrBI CCG'CTC
Cuts at: 50 630 1975 2029 3513 3678 50
Size: 580 1345 54 1484 165 55
Fragments arranged by size:
1484 1345 580 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1595 1595
Size: 3683
BsrFI r'CCGG_y
Cuts at: 1681 1862 2071 1681
Size: 181 209 3293
Fragments arranged by size:
3293 209 181
BssSI C'ACGA_G
Cuts at: 1954 2312 1954
Size: 358 3325
BstAPI GCAn_nnn'nTGC
Cuts at: 1060 1060
Size: 3683
BstBI TT'CG_AA
Cuts at: 2044 2044
Size: 3683
BstEII G'GTnAC_C
Cuts at: 607 607
Size: 3683
BstXI CCAn_nnnn'nTGG
Cuts at: 244 244
Size: 3683
BstYI r'GATC_y
Cuts at: 1 233 708 1175 1198 1533 1779 2791 2877
Size: 232 475 467 23 335 246 1012 86
Cuts at: 2877 2889 3235 1
Size: 12 346 449
Fragments arranged by size:
1012 475 467 449 346 335 246 232
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 597 597
Size: 3683
BtgI C'CryG_G
Cuts at: 107 220 107
Size: 113 3570
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1633 1814 1977 1633
Size: 181 163 3339
Fragments arranged by size:
3339 181 163
BtsI GCAGTG_nn'
Cuts at: 159 926 159
Size: 767 2916
DraI TTT'AAA
Cuts at: 682 682
Size: 3683
DrdI GACnn_nn'nnGTC
Cuts at: 1389 2247 1389
Size: 858 2825
EaeI y'GGCC_r
Cuts at: 125 407 1268 1442 1833 1860 3205 125
Size: 282 861 174 391 27 1345 603
Fragments arranged by size:
1345 861 603 391 282 174 27
EagI C'GGCC_G
Cuts at: 125 1268 125
Size: 1143 2540
EarI CTCTTCn'nnn_
Cuts at: 473 1706 1916 473
Size: 1233 210 2240
Fragments arranged by size:
2240 1233 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 179 1891 2201 2347 3493 179
Size: 1712 310 146 1146 369
Fragments arranged by size:
1712 1146 369 310 146
EcoICRI GAG'CTC
Cuts at: 695 695
Size: 3683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 266 497 1508 1940 2687 2955 201
Size: 65 231 1011 432 747 268 929
Fragments arranged by size:
1011 929 747 432 268 231 65
EcoO109I rG'GnC_Cy
Cuts at: 493 822 493
Size: 329 3354
EcoRI G'AATT_C
Cuts at: 687 687
Size: 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 339 1702 339
Size: 1363 2320
FspI TGC'GCA
Cuts at: 1464 1464
Size: 3683
HaeII r_GCGC'y
Cuts at: 1117 1365 2387 3366 1117
Size: 248 1022 979 1434
Fragments arranged by size:
1434 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 218 250 436 468 1525 1555 1557 1587 218
Size: 32 186 32 1057 30 2 30 2314
Fragments arranged by size:
2314 1057 186 32 32 30 30 2
HincII GTy'rAC
Cuts at: 722 722
Size: 3683
KasI G'GCGC_C
Cuts at: 1113 1361 1113
Size: 248 3435
KpnI G_GTAC'C
Cuts at: 703 703
Size: 3683
MluI A'CGCG_T
Cuts at: 3116 3116
Size: 3683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2538 2354
Size: 184 3499
MscI TGG'CCA
Cuts at: 409 1444 409
Size: 1035 2648
MslI CAynn'nnrTG
Cuts at: 1672 1799 1672
Size: 127 3556
MspA1I CmG'CkG
Cuts at: 794 1108 1468 2481 2726 794
Size: 314 360 1013 245 1751
Fragments arranged by size:
1751 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1864 1864
Size: 3683
NarI GG'CG_CC
Cuts at: 1114 1362 1114
Size: 248 3435
NcoI C'CATG_G
Cuts at: 107 107
Size: 3683
NgoMIV G'CCGG_C
Cuts at: 1862 1862
Size: 3683
NspI r_CATG'y
Cuts at: 736 1236 2143 736
Size: 500 907 2276
Fragments arranged by size:
2276 907 500
PasI CC'CwG_GG
Cuts at: 550 550
Size: 3683
PciI A'CATG_T
Cuts at: 2139 2139
Size: 3683
PflMI CCAn_nnn'nTGG
Cuts at: 525 525
Size: 3683
PmeI GTTT'AAAC
Cuts at: 682 682
Size: 3683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 3651
PpuMI rG'GwC_Cy
Cuts at: 493 493
Size: 3683
PstI C_TGCA'G
Cuts at: 730 1415 730
Size: 685 2998
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3683
PvuII CAG'CTG
Cuts at: 1108 1468 1108
Size: 360 3323
RsrII CG'GwC_CG
Cuts at: 1878 1878
Size: 3683
SacI G_AGCT'C
Cuts at: 697 697
Size: 3683
SalI G'TCGA_C
Cuts at: 720 720
Size: 3683
SapI GCTCTTCn'nnn_
Cuts at: 473 1706 1916 473
Size: 1233 210 2240
Fragments arranged by size:
2240 1233 210
SbfI CC_TGCA'GG
Cuts at: 730 730
Size: 3683
ScaI AGT'ACT
Cuts at: 404 3200 404
Size: 2796 887
SfcI C'TryA_G
Cuts at: 33 726 1411 2404 2595 33
Size: 693 685 993 191 1121
Fragments arranged by size:
1121 993 693 685 191
SfoI GGC'GCC
Cuts at: 1115 1363 1115
Size: 248 3435
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
SmaI CCC'GGG
Cuts at: 168 705 168
Size: 537 3146
SmlI C'TyrA_G
Cuts at: 635 648 838 1753 2245 2507 635
Size: 13 190 915 492 262 1811
Fragments arranged by size:
1811 915 492 262 190 13
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3683
SphI G_CATG'C
Cuts at: 736 1236 736
Size: 500 3183
StuI AGG'CCT
Cuts at: 186 186
Size: 3683
StyI C'CwwG_G
Cuts at: 107 229 286 582 817 1171 107
Size: 122 57 296 235 354 2619
Fragments arranged by size:
2619 354 296 235 122 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1266 1399 1858 2067 1266
Size: 133 459 209 2882
Fragments arranged by size:
2882 459 209 133
TatI w'GTAC_w
Cuts at: 402 3198 402
Size: 2796 887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 3651
Tth111I GACn'n_nGTC
Cuts at: 1480 1480
Size: 3683
XbaI T'CTAG_A
Cuts at: 714 714
Size: 3683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 304 1178 304
Size: 874 2809
XmaI C'CCGG_G
Cuts at: 166 703 166
Size: 537 3146
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI