Restriction map for
pF1KB7410
(Circular) (Six-base) MAPSORT of: pF1KB7410.seq Check: 472 from: 1 to: 4265
pF1KB7410 4265 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 921 921
Size: 4265
AccI GT'mk_AC
Cuts at: 1303 1303
Size: 4265
Acc65I G'GTAC_C
Cuts at: 1281 1281
Size: 4265
AclI AA'CG_TT
Cuts at: 4105 4105
Size: 4265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 2090 2522 3269 3537 746
Size: 1344 432 747 268 1474
Fragments arranged by size:
1474 1344 747 432 268
AflIII A'CryG_T
Cuts at: 754 1617 2721 3698 754
Size: 863 1104 977 1321
Fragments arranged by size:
1321 1104 977 863
AgeI A'CCGG_T
Cuts at: 2653 2653
Size: 4265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1144 1176 1144
Size: 32 4233
AlwNI CAG_nnn'CTG
Cuts at: 255 1570 3137 255
Size: 1315 1567 1383
Fragments arranged by size:
1567 1383 1315
ApaLI G'TGCA_C
Cuts at: 3035 3035
Size: 4265
ApoI r'AATT_y
Cuts at: 610 1269 3618 3874 610
Size: 659 2349 256 1001
Fragments arranged by size:
2349 1001 659 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4265
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
AvaI C'yCGr_G
Cuts at: 117 1285 117
Size: 1168 3097
BamHI G'GATC_C
Cuts at: 1290 1290
Size: 4265
BanI G'GyrC_C
Cuts at: 122 647 1281 1695 1943 1978 122
Size: 525 634 414 248 35 2409
Fragments arranged by size:
2409 634 525 414 248 35
BanII G_rGCy'C
Cuts at: 302 424 1279 2309 302
Size: 122 855 1030 2258
Fragments arranged by size:
2258 1030 855 122
BbeI G_GCGC'C
Cuts at: 1699 1947 1699
Size: 248 4017
BbsI GAAGACnn'nnnn_
Cuts at: 219 344 891 219
Size: 125 547 3593
Fragments arranged by size:
3593 547 125
BciVI GTATCCnnnnn_n'
Cuts at: 538 1083 2163 2924 4265 538
Size: 545 1080 761 1341 538
Fragments arranged by size:
1341 1080 761 545 538
BclI T'GATC_A
Cuts at: 1785 1785
Size: 4265
BglII A'GATC_T
Cuts at: 796 1780 3817 796
Size: 984 2037 1244
Fragments arranged by size:
2037 1244 984
BlpI GC'TnA_GC
Cuts at: 1377 1377
Size: 4265
Bme1580I G_kGCm'C
Cuts at: 125 652 1890 1983 3039 125
Size: 527 1238 93 1056 1351
Fragments arranged by size:
1351 1238 1056 527 93
BmrI ACTGGGnnnn_n'
Cuts at: 1477 1558 1893 4037 1477
Size: 81 335 2144 1705
Fragments arranged by size:
2144 1705 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 4265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 340
Size: 4265
Bpu10I CC'TnA_GC
Cuts at: 418 418
Size: 4265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2320 2812 3110 1441
Size: 879 492 298 2596
Fragments arranged by size:
2596 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 120 3969 120
Size: 3849 416
BsaAI yAC'GTr
Cuts at: 1618 1618
Size: 4265
BsaBI GATnn'nnATC
Cuts at: 6 715 1779 3816 6
Size: 709 1064 2037 455
Fragments arranged by size:
2037 1064 709 455
BsaHI Gr'CG_yC
Cuts at: 1696 1944 2646 4142 1696
Size: 248 702 1496 1819
Fragments arranged by size:
1819 1496 702 248
BsaWI w'CCGG_w
Cuts at: 675 1454 1975 2653 2927 3074 3485 675
Size: 779 521 678 274 147 411 1455
Fragments arranged by size:
1455 779 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 338 368 1012 1042 1144 1174 338
Size: 30 644 30 102 30 3429
Fragments arranged by size:
3429 644 102 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 625 867 310
Size: 315 242 3708
Fragments arranged by size:
3708 315 242
BseYI C'CCAG_C
Cuts at: 1690 2448 3025 3521 1690
Size: 758 577 496 2434
Fragments arranged by size:
2434 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 1016 423
Size: 593 3672
BsiEI CG_ry'CG
Cuts at: 104 1807 1853 3061 104
Size: 1703 46 1208 1308
Fragments arranged by size:
1703 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 302 424 1279 2057 2247 3039 302
Size: 122 855 778 190 792 1528
Fragments arranged by size:
1528 855 792 778 190 122
BsmI GAATG_Cn'
Cuts at: 1047 1047
Size: 4265
Bsp1286I G_dGCh'C
Cuts at: 125 302 424 652 1279 1890 1983 2057 2247
Size: 177 122 228 627 611 93 74 190
Cuts at: 2247 2309 3039 125
Size: 62 730 1351
Fragments arranged by size:
1351 730 627 611 228 190 177 122
93 74 62
BspEI T'CCGG_A
Cuts at: 3485 3485
Size: 4265
BspHI T'CATG_A
Cuts at: 3441 3441
Size: 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 921 1315 1529 1831 2212 921
Size: 394 214 302 381 2974
Fragments arranged by size:
2974 394 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2557 2611 4095 4260 50
Size: 2507 54 1484 165 55
Fragments arranged by size:
2507 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 962 1163 2177 962
Size: 201 1014 3050
Fragments arranged by size:
3050 1014 201
BsrFI r'CCGG_y
Cuts at: 2263 2444 2653 2263
Size: 181 209 3875
Fragments arranged by size:
3875 209 181
BssSI C'ACGA_G
Cuts at: 861 2536 2894 861
Size: 1675 358 2232
Fragments arranged by size:
2232 1675 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1642 1642
Size: 4265
BstBI TT'CG_AA
Cuts at: 2626 2626
Size: 4265
BstEII G'GTnAC_C
Cuts at: 198 198
Size: 4265
BstYI r'GATC_y
Cuts at: 1 351 796 1290 1757 1780 2115 2361 3373
Size: 350 445 494 467 23 335 246 1012
Cuts at: 3373 3459 3471 3817 1
Size: 86 12 346 449
Fragments arranged by size:
1012 494 467 449 445 350 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 455 845 107
Size: 348 390 3527
Fragments arranged by size:
3527 390 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2215 2396 2559 2215
Size: 181 163 3921
Fragments arranged by size:
3921 181 163
BtsI GCAGTG_nn'
Cuts at: 327 823 1508 327
Size: 496 685 3084
Fragments arranged by size:
3084 685 496
DraI TTT'AAA
Cuts at: 1264 1264
Size: 4265
DrdI GACnn_nn'nnGTC
Cuts at: 250 1971 2829 250
Size: 1721 858 1686
Fragments arranged by size:
1721 1686 858
EaeI y'GGCC_r
Cuts at: 246 1850 2024 2415 2442 3787 246
Size: 1604 174 391 27 1345 724
Fragments arranged by size:
1604 1345 724 391 174 27
EagI C'GGCC_G
Cuts at: 1850 1850
Size: 4265
EarI CTCTTCn'nnn_
Cuts at: 172 667 722 2288 2498 172
Size: 495 55 1566 210 1939
Fragments arranged by size:
1939 1566 495 210 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2473 2783 2929 4075 2473
Size: 310 146 1146 2663
Fragments arranged by size:
2663 1146 310 146
EcoICRI GAG'CTC
Cuts at: 300 422 1277 300
Size: 122 855 3288
Fragments arranged by size:
3288 855 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 746 2090 2522 3269 3537 340
Size: 406 1344 432 747 268 1068
Fragments arranged by size:
1344 1068 747 432 406 268
EcoO109I rG'GnC_Cy
Cuts at: 153 252 739 1404 153
Size: 99 487 665 3014
Fragments arranged by size:
3014 665 487 99
EcoRI G'AATT_C
Cuts at: 1269 1269
Size: 4265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 4265
FspI TGC'GCA
Cuts at: 2046 2046
Size: 4265
HaeII r_GCGC'y
Cuts at: 1699 1947 2969 3948 1699
Size: 248 1022 979 2016
Fragments arranged by size:
2016 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2107 2137 2139 2169 2107
Size: 30 2 30 4203
Fragments arranged by size:
4203 30 30 2
HincII GTy'rAC
Cuts at: 1304 1304
Size: 4265
KasI G'GCGC_C
Cuts at: 1695 1943 1695
Size: 248 4017
KpnI G_GTAC'C
Cuts at: 1285 1285
Size: 4265
MluI A'CGCG_T
Cuts at: 3698 3698
Size: 4265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 903 923 1010 1125 2936 3120 903
Size: 20 87 115 1811 184 2048
Fragments arranged by size:
2048 1811 184 115 87 20
MscI TGG'CCA
Cuts at: 2026 2026
Size: 4265
MslI CAynn'nnrTG
Cuts at: 2254 2381 2254
Size: 127 4138
MspA1I CmG'CkG
Cuts at: 1000 1376 1690 2050 3063 3308 1000
Size: 376 314 360 1013 245 1957
Fragments arranged by size:
1957 1013 376 360 314 245
NaeI GCC'GGC
Cuts at: 2446 2446
Size: 4265
NarI GG'CG_CC
Cuts at: 1696 1944 1696
Size: 248 4017
NcoI C'CATG_G
Cuts at: 107 845 107
Size: 738 3527
NgoMIV G'CCGG_C
Cuts at: 2444 2444
Size: 4265
NspI r_CATG'y
Cuts at: 510 758 1318 1818 2725 510
Size: 248 560 500 907 2050
Fragments arranged by size:
2050 907 560 500 248
PasI CC'CwG_GG
Cuts at: 156 156
Size: 4265
PciI A'CATG_T
Cuts at: 754 2721 754
Size: 1967 2298
PflMI CCAn_nnn'nTGG
Cuts at: 255 407 255
Size: 152 4113
PmeI GTTT'AAAC
Cuts at: 1264 1264
Size: 4265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1144 1176 3404 3436 1144
Size: 32 2228 32 1973
Fragments arranged by size:
2228 1973 32 32
PpuMI rG'GwC_Cy
Cuts at: 252 252
Size: 4265
PsiI TTA'TAA
Cuts at: 596 596
Size: 4265
PstI C_TGCA'G
Cuts at: 307 322 371 778 1312 1997 307
Size: 15 49 407 534 685 2575
Fragments arranged by size:
2575 685 534 407 49 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4265
PvuII CAG'CTG
Cuts at: 1000 1690 2050 1000
Size: 690 360 3215
Fragments arranged by size:
3215 690 360
RsrII CG'GwC_CG
Cuts at: 2460 2460
Size: 4265
SacI G_AGCT'C
Cuts at: 302 424 1279 302
Size: 122 855 3288
Fragments arranged by size:
3288 855 122
SalI G'TCGA_C
Cuts at: 1302 1302
Size: 4265
SapI GCTCTTCn'nnn_
Cuts at: 172 722 2288 2498 172
Size: 550 1566 210 1939
Fragments arranged by size:
1939 1566 550 210
SbfI CC_TGCA'GG
Cuts at: 1312 1312
Size: 4265
ScaI AGT'ACT
Cuts at: 499 3782 499
Size: 3283 982
SfcI C'TryA_G
Cuts at: 33 303 318 367 774 1308 1993 2986 3177
Size: 270 15 49 407 534 685 993 191
Cuts at: 3177 33
Size: 1121
Fragments arranged by size:
1121 993 685 534 407 270 191 49
15
SfoI GGC'GCC
Cuts at: 1697 1945 1697
Size: 248 4017
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
SmaI CCC'GGG
Cuts at: 1287 1287
Size: 4265
SmlI C'TyrA_G
Cuts at: 1420 2335 2827 3089 1420
Size: 915 492 262 2596
Fragments arranged by size:
2596 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4265
SphI G_CATG'C
Cuts at: 510 1318 1818 510
Size: 808 500 2957
Fragments arranged by size:
2957 808 500
StyI C'CwwG_G
Cuts at: 107 845 1399 1753 107
Size: 738 554 354 2619
Fragments arranged by size:
2619 738 554 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 205 1848 1981 2440 2649 205
Size: 1643 133 459 209 1821
Fragments arranged by size:
1821 1643 459 209 133
TatI w'GTAC_w
Cuts at: 497 3780 497
Size: 3283 982
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 917 949 3782 3814 917
Size: 32 2833 32 1368
Fragments arranged by size:
2833 1368 32 32
Tth111I GACn'n_nGTC
Cuts at: 2062 2062
Size: 4265
XbaI T'CTAG_A
Cuts at: 1296 1296
Size: 4265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1760 1760
Size: 4265
XmaI C'CCGG_G
Cuts at: 1285 1285
Size: 4265
XmnI GAAnn'nnTTC
Cuts at: 731 1045 731
Size: 314 3951
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI