Restriction map for
pF1KB7411
(Circular) (Six-base) MAPSORT of: pF1KB7411.seq Check: 938 from: 1 to: 4055
pF1KB7411 4055 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 710 710
Size: 4055
AccI GT'mk_AC
Cuts at: 655 1093 655
Size: 438 3617
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1880 2312 3059 3327 166
Size: 1714 432 747 268 894
Fragments arranged by size:
1714 894 747 432 268
AflIII A'CryG_T
Cuts at: 1407 2511 3488 1407
Size: 1104 977 1974
Fragments arranged by size:
1974 1104 977
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AleI CACnn'nnGTG
Cuts at: 433 433
Size: 4055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 517 517
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 1360 2927 1360
Size: 1567 2488
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 274 1059 3408 3664 274
Size: 785 2349 256 665
Fragments arranged by size:
2349 785 665 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 812 1071 1485 1733 1768 812
Size: 259 414 248 35 3099
Fragments arranged by size:
3099 414 259 248 35
BanII G_rGCy'C
Cuts at: 143 1069 2099 143
Size: 926 1030 2099
Fragments arranged by size:
2099 1030 926
BbeI G_GCGC'C
Cuts at: 816 1489 1737 816
Size: 673 248 3134
Fragments arranged by size:
3134 673 248
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 248 749 1575 248
Size: 501 826 2728
Fragments arranged by size:
2728 826 501
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 540 1680 1773 2829 540
Size: 1140 93 1056 1766
Fragments arranged by size:
1766 1140 1056 93
BmgBI CAC'GTC
Cuts at: 1002 1002
Size: 4055
BmrI ACTGGGnnnn_n'
Cuts at: 1267 1348 1683 3827 1267
Size: 81 335 2144 1495
Fragments arranged by size:
2144 1495 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 1231 2110 2602 2900 379
Size: 852 879 492 298 1534
Fragments arranged by size:
1534 879 852 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 1408 1408
Size: 4055
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 813 1486 1734 2436 3932 813
Size: 673 248 702 1496 936
Fragments arranged by size:
1496 936 702 673 248
BsaWI w'CCGG_w
Cuts at: 605 1244 1765 2443 2717 2864 3275 605
Size: 639 521 678 274 147 411 1385
Fragments arranged by size:
1385 678 639 521 411 274 147
BseYI C'CCAG_C
Cuts at: 550 1480 2238 2815 3311 550
Size: 930 758 577 496 1294
Fragments arranged by size:
1294 930 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 411
Size: 4055
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1069 1847 2037 2829 1069
Size: 778 190 792 2295
Fragments arranged by size:
2295 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 143 540 1069 1680 1773 1847 2037 2099 2829
Size: 397 529 611 93 74 190 62 730
Cuts at: 2829 143
Size: 1369
Fragments arranged by size:
1369 730 611 529 397 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 3231 3231
Size: 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 710 1105 1319 1621 2002 710
Size: 395 214 302 381 2763
Fragments arranged by size:
2763 395 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 2053 2234 2443 2053
Size: 181 209 3665
Fragments arranged by size:
3665 209 181
BsrGI T'GTAC_A
Cuts at: 739 739
Size: 4055
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 1432 1432
Size: 4055
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4055
BstYI r'GATC_y
Cuts at: 1 1080 1547 1570 1905 2151 3163 3249 3261
Size: 1079 467 23 335 246 1012 86 12
Cuts at: 3261 3607 1
Size: 346 449
Fragments arranged by size:
1079 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 656 656
Size: 4055
Bsu36I CC'TnA_GG
Cuts at: 988 988
Size: 4055
BtgI C'CryG_G
Cuts at: 867 867
Size: 4055
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 428 467 1298 428
Size: 39 831 3185
Fragments arranged by size:
3185 831 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 628 663 628
Size: 35 4020
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 485 515 1640 1814 2205 2232 3577 485
Size: 30 1125 174 391 27 1345 963
Fragments arranged by size:
1345 1125 963 391 174 30 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 199 339 2078 2288 199
Size: 140 1739 210 1966
Fragments arranged by size:
1966 1739 210 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1880 2312 3059 3327 166
Size: 1714 432 747 268 894
Fragments arranged by size:
1714 894 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1194 1194
Size: 4055
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 4055
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 816 1489 1737 2759 3738 816
Size: 673 248 1022 979 1133
Fragments arranged by size:
1133 1022 979 673 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1897 1927 1929 1959 1897
Size: 30 2 30 3993
Fragments arranged by size:
3993 30 30 2
HincII GTy'rAC
Cuts at: 957 1094 957
Size: 137 3918
HindIII A'AGCT_T
Cuts at: 178 178
Size: 4055
KasI G'GCGC_C
Cuts at: 812 1485 1733 812
Size: 673 248 3134
Fragments arranged by size:
3134 673 248
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2726 2910 2726
Size: 184 3871
MscI TGG'CCA
Cuts at: 487 517 1816 487
Size: 30 1299 2726
Fragments arranged by size:
2726 1299 30
MslI CAynn'nnrTG
Cuts at: 112 286 433 670 778 2044 2171 112
Size: 174 147 237 108 1266 127 1996
Fragments arranged by size:
1996 1266 237 174 147 127 108
MspA1I CmG'CkG
Cuts at: 1166 1480 1840 2853 3098 1166
Size: 314 360 1013 245 2123
Fragments arranged by size:
2123 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 813 1486 1734 813
Size: 673 248 3134
Fragments arranged by size:
3134 673 248
NcoI C'CATG_G
Cuts at: 867 867
Size: 4055
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NspI r_CATG'y
Cuts at: 573 1108 1608 2515 573
Size: 535 500 907 2113
Fragments arranged by size:
2113 907 535 500
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PstI C_TGCA'G
Cuts at: 1102 1787 1102
Size: 685 3370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 1480 1840 1480
Size: 360 3695
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 415 3572 415
Size: 3157 898
SfcI C'TryA_G
Cuts at: 33 981 1098 1783 2776 2967 33
Size: 948 117 685 993 191 1121
Fragments arranged by size:
1121 993 948 685 191 117
SfoI GGC'GCC
Cuts at: 814 1487 1735 814
Size: 673 248 3134
Fragments arranged by size:
3134 673 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 358 1210 2125 2617 2879 358
Size: 852 915 492 262 1534
Fragments arranged by size:
1534 915 852 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StyI C'CwwG_G
Cuts at: 240 581 867 1189 1543 240
Size: 341 286 322 354 2752
Fragments arranged by size:
2752 354 341 322 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1638 1771 2230 2439 1638
Size: 133 459 209 3254
Fragments arranged by size:
3254 459 209 133
TatI w'GTAC_w
Cuts at: 290 413 739 786 3570 290
Size: 123 326 47 2784 775
Fragments arranged by size:
2784 775 326 123 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4023
Tth111I GACn'n_nGTC
Cuts at: 444 1852 444
Size: 1408 2647
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1550 1550
Size: 4055
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BpmI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BssHII BstEII
ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI