Restriction map for
pF1KB7412
(Circular) (Six-base) MAPSORT of: pF1KB7412.seq Check: 6932 from: 1 to: 4130
pF1KB7412 4130 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AatII G_ACGT'C
Cuts at: 953 953
Size: 4130
AccI GT'mk_AC
Cuts at: 1168 1168
Size: 4130
Acc65I G'GTAC_C
Cuts at: 1146 1146
Size: 4130
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 986 1103 1955 2387 3134 3402 485
Size: 501 117 852 432 747 268 1213
Fragments arranged by size:
1213 852 747 501 432 268 117
AflIII A'CryG_T
Cuts at: 1482 2586 3563 1482
Size: 1104 977 2049
Fragments arranged by size:
2049 1104 977
AgeI A'CCGG_T
Cuts at: 2518 2518
Size: 4130
AleI CACnn'nnGTG
Cuts at: 137 137
Size: 4130
AlwNI CAG_nnn'CTG
Cuts at: 1435 3002 1435
Size: 1567 2563
ApaLI G'TGCA_C
Cuts at: 2900 2900
Size: 4130
ApoI r'AATT_y
Cuts at: 231 800 834 987 1050 1134 3483 3739 231
Size: 569 34 153 63 84 2349 256 622
Fragments arranged by size:
2349 622 569 256 153 84 63 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 4130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 1150 1150
Size: 4130
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 852 885 852
Size: 33 4097
BamHI G'GATC_C
Cuts at: 894 1155 894
Size: 261 3869
BanI G'GyrC_C
Cuts at: 250 396 578 1032 1063 1146 1560 1808 1843
Size: 146 182 454 31 83 414 248 35
Cuts at: 1843 250
Size: 2537
Fragments arranged by size:
2537 454 414 248 182 146 83 35
31
BanII G_rGCy'C
Cuts at: 471 1144 2174 471
Size: 673 1030 2427
Fragments arranged by size:
2427 1030 673
BbeI G_GCGC'C
Cuts at: 254 400 582 1564 1812 254
Size: 146 182 982 248 2572
Fragments arranged by size:
2572 982 248 182 146
BbsI GAAGACnn'nnnn_
Cuts at: 877 877
Size: 4130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4096
BciVI GTATCCnnnnn_n'
Cuts at: 880 2028 2789 4130 880
Size: 1148 761 1341 880
Fragments arranged by size:
1341 1148 880 761
BclI T'GATC_A
Cuts at: 1650 1650
Size: 4130
BglII A'GATC_T
Cuts at: 1645 3682 1645
Size: 2037 2093
BlpI GC'TnA_GC
Cuts at: 1242 1242
Size: 4130
Bme1580I G_kGCm'C
Cuts at: 367 1035 1755 1848 2904 367
Size: 668 720 93 1056 1593
Fragments arranged by size:
1593 1056 720 668 93
BmrI ACTGGGnnnn_n'
Cuts at: 156 340 1124 1342 1423 1758 3902 156
Size: 184 784 218 81 335 2144 384
Fragments arranged by size:
2144 784 384 335 218 184 81
BmtI G_CTAG'C
Cuts at: 344 344
Size: 4130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 722 2497 722
Size: 1775 2355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 977 730
Size: 247 3883
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 951 1306 2185 2677 2975 951
Size: 355 879 492 298 2106
Fragments arranged by size:
2106 879 492 355 298
BsaI GGTCTCn'nnnn_
Cuts at: 3834 3834
Size: 4130
BsaAI yAC'GTr
Cuts at: 1483 1483
Size: 4130
BsaBI GATnn'nnATC
Cuts at: 6 1644 3681 6
Size: 1638 2037 455
Fragments arranged by size:
2037 1638 455
BsaHI Gr'CG_yC
Cuts at: 251 397 579 950 1561 1809 2511 4007 251
Size: 146 182 371 611 248 702 1496 374
Fragments arranged by size:
1496 702 611 374 371 248 182 146
BsaWI w'CCGG_w
Cuts at: 1319 1840 2518 2792 2939 3350 1319
Size: 521 678 274 147 411 2099
Fragments arranged by size:
2099 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 703 733 703
Size: 30 4100
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 163
Size: 4130
BseYI C'CCAG_C
Cuts at: 115 1555 2313 2890 3386 115
Size: 1440 758 577 496 859
Fragments arranged by size:
1440 859 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 125 299 1672 1718 2926 104
Size: 21 174 1373 46 1208 1308
Fragments arranged by size:
1373 1308 1208 174 46 21
BsiHKAI G_wGCw'C
Cuts at: 431 538 1144 1922 2112 2904 431
Size: 107 606 778 190 792 1657
Fragments arranged by size:
1657 792 778 606 190 107
Bsp1286I G_dGCh'C
Cuts at: 367 431 471 538 1035 1144 1755 1848 1922
Size: 64 40 67 497 109 611 93 74
Cuts at: 1922 2112 2174 2904 367
Size: 190 62 730 1593
Fragments arranged by size:
1593 730 611 497 190 109 93 74
67 64 62 40
BspEI T'CCGG_A
Cuts at: 3350 3350
Size: 4130
BspHI T'CATG_A
Cuts at: 3306 3306
Size: 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1180 1394 1696 2077 1180
Size: 214 302 381 3233
Fragments arranged by size:
3233 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2422 2476 3960 4125 50
Size: 2372 54 1484 165 55
Fragments arranged by size:
2372 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2042 2042
Size: 4130
BsrFI r'CCGG_y
Cuts at: 136 2128 2309 2518 136
Size: 1992 181 209 1748
Fragments arranged by size:
1992 1748 209 181
BssHII G'CGCG_C
Cuts at: 317 317
Size: 4130
BssSI C'ACGA_G
Cuts at: 2401 2759 2401
Size: 358 3772
BstAPI GCAn_nnn'nTGC
Cuts at: 1507 1507
Size: 4130
BstBI TT'CG_AA
Cuts at: 2491 2491
Size: 4130
BstXI CCAn_nnnn'nTGG
Cuts at: 774 774
Size: 4130
BstYI r'GATC_y
Cuts at: 1 894 1155 1622 1645 1980 2226 3238 3324
Size: 893 261 467 23 335 246 1012 86
Cuts at: 3324 3336 3682 1
Size: 12 346 449
Fragments arranged by size:
1012 893 467 449 346 335 261 246
86 23 12
BtgI C'CryG_G
Cuts at: 311 311
Size: 4130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2080 2261 2424 2080
Size: 181 163 3786
Fragments arranged by size:
3786 181 163
BtsI GCAGTG_nn'
Cuts at: 593 1054 1373 593
Size: 461 319 3350
Fragments arranged by size:
3350 461 319
DraI TTT'AAA
Cuts at: 1129 1129
Size: 4130
DrdI GACnn_nn'nnGTC
Cuts at: 1836 2694 1836
Size: 858 3272
EaeI y'GGCC_r
Cuts at: 305 1715 1889 2280 2307 3652 305
Size: 1410 174 391 27 1345 783
Fragments arranged by size:
1410 1345 783 391 174 27
EagI C'GGCC_G
Cuts at: 1715 1715
Size: 4130
EarI CTCTTCn'nnn_
Cuts at: 605 669 2153 2363 605
Size: 64 1484 210 2372
Fragments arranged by size:
2372 1484 210 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 846 2338 2648 2794 3940 846
Size: 1492 310 146 1146 1036
Fragments arranged by size:
1492 1146 1036 310 146
EcoICRI GAG'CTC
Cuts at: 1142 1142
Size: 4130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 730 977 986 1103 1955 2387 3134 3402
Size: 245 247 9 117 852 432 747 268
Cuts at: 3402 485
Size: 1213
Fragments arranged by size:
1213 852 747 432 268 247 245 117
9
EcoNI CCTnn'n_nnAGG
Cuts at: 634 634
Size: 4130
EcoO109I rG'GnC_Cy
Cuts at: 569 1269 569
Size: 700 3430
EcoRI G'AATT_C
Cuts at: 834 987 1134 834
Size: 153 147 3830
Fragments arranged by size:
3830 153 147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 901 2149 901
Size: 1248 2882
FspI TGC'GCA
Cuts at: 1911 1911
Size: 4130
HaeII r_GCGC'y
Cuts at: 254 400 508 582 1564 1812 2834 3813 254
Size: 146 108 74 982 248 1022 979 571
Fragments arranged by size:
1022 982 979 571 248 146 108 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 886 918 1972 2002 2004 2034 886
Size: 32 1054 30 2 30 2982
Fragments arranged by size:
2982 1054 32 30 30 2
HincII GTy'rAC
Cuts at: 1169 1169
Size: 4130
KasI G'GCGC_C
Cuts at: 250 396 578 1560 1808 250
Size: 146 182 982 248 2572
Fragments arranged by size:
2572 982 248 182 146
KpnI G_GTAC'C
Cuts at: 1150 1150
Size: 4130
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 884 2801 2985 884
Size: 1917 184 2029
Fragments arranged by size:
2029 1917 184
MscI TGG'CCA
Cuts at: 307 1891 307
Size: 1584 2546
MslI CAynn'nnrTG
Cuts at: 137 2119 2246 137
Size: 1982 127 2021
Fragments arranged by size:
2021 1982 127
MspA1I CmG'CkG
Cuts at: 187 313 1241 1555 1915 2928 3173 187
Size: 126 928 314 360 1013 245 1144
Fragments arranged by size:
1144 1013 928 360 314 245 126
NaeI GCC'GGC
Cuts at: 2311 2311
Size: 4130
NarI GG'CG_CC
Cuts at: 251 397 579 1561 1809 251
Size: 146 182 982 248 2572
Fragments arranged by size:
2572 982 248 182 146
NgoMIV G'CCGG_C
Cuts at: 2309 2309
Size: 4130
NheI G'CTAG_C
Cuts at: 340 340
Size: 4130
NspI r_CATG'y
Cuts at: 621 1183 1683 2590 621
Size: 562 500 907 2161
Fragments arranged by size:
2161 907 562 500
PciI A'CATG_T
Cuts at: 2586 2586
Size: 4130
PfoI T'CCnGG_A
Cuts at: 1016 1016
Size: 4130
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 4098
PpuMI rG'GwC_Cy
Cuts at: 569 569
Size: 4130
PstI C_TGCA'G
Cuts at: 272 1177 1862 272
Size: 905 685 2540
Fragments arranged by size:
2540 905 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 1555 1915 1555
Size: 360 3770
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 1144 1144
Size: 4130
SacII CC_GC'GG
Cuts at: 314 314
Size: 4130
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 669 2153 2363 669
Size: 1484 210 2436
Fragments arranged by size:
2436 1484 210
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 3647 3647
Size: 4130
SfcI C'TryA_G
Cuts at: 33 268 1173 1858 2851 3042 33
Size: 235 905 685 993 191 1121
Fragments arranged by size:
1121 993 905 685 235 191
SfoI GGC'GCC
Cuts at: 252 398 580 1562 1810 252
Size: 146 182 982 248 2572
Fragments arranged by size:
2572 982 248 182 146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SgrAI Cr'CCGG_yG
Cuts at: 136 136
Size: 4130
SmaI CCC'GGG
Cuts at: 1152 1152
Size: 4130
SmlI C'TyrA_G
Cuts at: 966 1285 2200 2692 2954 966
Size: 319 915 492 262 2142
Fragments arranged by size:
2142 915 492 319 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4130
SphI G_CATG'C
Cuts at: 621 1183 1683 621
Size: 562 500 3068
Fragments arranged by size:
3068 562 500
StyI C'CwwG_G
Cuts at: 1264 1618 1264
Size: 354 3776
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 494 767 1713 1846 2305 2514 494
Size: 273 946 133 459 209 2110
Fragments arranged by size:
2110 946 459 273 209 133
TatI w'GTAC_w
Cuts at: 1107 3645 1107
Size: 2538 1592
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 585 617 3647 3679 585
Size: 32 3030 32 1036
Fragments arranged by size:
3030 1036 32 32
Tth111I GACn'n_nGTC
Cuts at: 1927 1927
Size: 4130
XbaI T'CTAG_A
Cuts at: 1161 1161
Size: 4130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1625 1625
Size: 4130
XmaI C'CCGG_G
Cuts at: 1150 1150
Size: 4130
ZraI GAC'GTC
Cuts at: 951 951
Size: 4130
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BbvCI BfrBI BglI BmgBI Bpu10I BsgI BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI