(Circular) (Six-base) MAPSORT of: pF1KB7413.seq Check: 8371 from: 1 to: 4268 pF1KB7413 4268 bp With 182 enzymes: SgfI * June 20, 2008 10:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 770 770 Size: 4268 AccI GT'mk_AC Cuts at: 345 1306 345 Size: 961 3307 Acc65I G'GTAC_C Cuts at: 1284 1284 Size: 4268 AclI AA'CG_TT Cuts at: 4108 4108 Size: 4268 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1131 2093 2525 3272 3540 1131 Size: 962 432 747 268 1859 Fragments arranged by size: 1859 962 747 432 268 AflIII A'CryG_T Cuts at: 1620 2724 3701 1620 Size: 1104 977 2187 Fragments arranged by size: 2187 1104 977 AgeI A'CCGG_T Cuts at: 2656 2656 Size: 4268 AhdI GACnn_n'nnGTC Cuts at: 906 906 Size: 4268 AlwNI CAG_nnn'CTG Cuts at: 648 865 898 1171 1573 3140 648 Size: 217 33 273 402 1567 1776 Fragments arranged by size: 1776 1567 402 273 217 33 ApaI G_GGCC'C Cuts at: 1053 1053 Size: 4268 ApaLI G'TGCA_C Cuts at: 659 3038 659 Size: 2379 1889 ApoI r'AATT_y Cuts at: 1272 3621 3877 1272 Size: 2349 256 1663 Fragments arranged by size: 2349 1663 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4268 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4268 AvaI C'yCGr_G Cuts at: 702 983 1288 702 Size: 281 305 3682 Fragments arranged by size: 3682 305 281 BamHI G'GATC_C Cuts at: 1293 1293 Size: 4268 BanI G'GyrC_C Cuts at: 615 804 1118 1284 1698 1946 1981 615 Size: 189 314 166 414 248 35 2902 Fragments arranged by size: 2902 414 314 248 189 166 35 BanII G_rGCy'C Cuts at: 818 993 1053 1282 2312 818 Size: 175 60 229 1030 2774 Fragments arranged by size: 2774 1030 229 175 60 BbeI G_GCGC'C Cuts at: 808 1702 1950 808 Size: 894 248 3126 Fragments arranged by size: 3126 894 248 BbsI GAAGACnn'nnnn_ Cuts at: 566 1223 566 Size: 657 3611 BbvCI CC'TCA_GC Cuts at: 1017 1017 Size: 4268 BciVI GTATCCnnnnn_n' Cuts at: 2166 2927 4268 2166 Size: 761 1341 2166 Fragments arranged by size: 2166 1341 761 BclI T'GATC_A Cuts at: 575 1788 575 Size: 1213 3055 BglI GCCn_nnn'nGGC Cuts at: 734 734 Size: 4268 BglII A'GATC_T Cuts at: 1783 3820 1783 Size: 2037 2231 BlpI GC'TnA_GC Cuts at: 395 691 1380 395 Size: 296 689 3283 Fragments arranged by size: 3283 689 296 Bme1580I G_kGCm'C Cuts at: 663 1053 1121 1893 1986 3042 663 Size: 390 68 772 93 1056 1889 Fragments arranged by size: 1889 1056 772 390 93 68 BmrI ACTGGGnnnn_n' Cuts at: 177 1480 1561 1896 4040 177 Size: 1303 81 335 2144 405 Fragments arranged by size: 2144 1303 405 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 526 2635 526 Size: 2109 2159 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 613 832 848 613 Size: 219 16 4033 Fragments arranged by size: 4033 219 16 Bpu10I CC'TnA_GC Cuts at: 620 1017 620 Size: 397 3871 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1444 2323 2815 3113 1444 Size: 879 492 298 2599 Fragments arranged by size: 2599 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3972 3972 Size: 4268 BsaAI yAC'GTr Cuts at: 606 1621 606 Size: 1015 3253 BsaBI GATnn'nnATC Cuts at: 6 1782 3819 6 Size: 1776 2037 455 Fragments arranged by size: 2037 1776 455 BsaHI Gr'CG_yC Cuts at: 805 1699 1947 2649 4145 805 Size: 894 248 702 1496 928 Fragments arranged by size: 1496 928 894 702 248 BsaWI w'CCGG_w Cuts at: 1457 1978 2656 2930 3077 3488 1457 Size: 521 678 274 147 411 2237 Fragments arranged by size: 2237 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 438 438 Size: 4268 BseYI C'CCAG_C Cuts at: 324 641 858 1114 1693 2451 3028 3524 324 Size: 317 217 256 579 758 577 496 1068 Fragments arranged by size: 1068 758 579 577 496 317 256 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 275 439 275 Size: 164 4104 BsiEI CG_ry'CG Cuts at: 104 1810 1856 3064 104 Size: 1706 46 1208 1308 Fragments arranged by size: 1706 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 663 993 1282 2060 2250 3042 663 Size: 330 289 778 190 792 1889 Fragments arranged by size: 1889 792 778 330 289 190 BsmI GAATG_Cn' Cuts at: 249 249 Size: 4268 Bsp1286I G_dGCh'C Cuts at: 663 818 993 1053 1121 1282 1893 1986 2060 Size: 155 175 60 68 161 611 93 74 Cuts at: 2060 2250 2312 3042 663 Size: 190 62 730 1889 Fragments arranged by size: 1889 730 611 190 175 161 155 93 74 68 62 60 BspEI T'CCGG_A Cuts at: 3488 3488 Size: 4268 BspHI T'CATG_A Cuts at: 3444 3444 Size: 4268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 770 1318 1532 1834 2215 770 Size: 548 214 302 381 2823 Fragments arranged by size: 2823 548 381 302 214 BsrBI CCG'CTC Cuts at: 50 2560 2614 4098 4263 50 Size: 2510 54 1484 165 55 Fragments arranged by size: 2510 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2180 2180 Size: 4268 BsrFI r'CCGG_y Cuts at: 2266 2447 2656 2266 Size: 181 209 3878 Fragments arranged by size: 3878 209 181 BsrGI T'GTAC_A Cuts at: 471 471 Size: 4268 BssSI C'ACGA_G Cuts at: 884 2539 2897 884 Size: 1655 358 2255 Fragments arranged by size: 2255 1655 358 BstAPI GCAn_nnn'nTGC Cuts at: 1645 1645 Size: 4268 BstBI TT'CG_AA Cuts at: 2629 2629 Size: 4268 BstXI CCAn_nnnn'nTGG Cuts at: 766 766 Size: 4268 BstYI r'GATC_y Cuts at: 1 210 491 1293 1760 1783 2118 2364 3376 Size: 209 281 802 467 23 335 246 1012 Cuts at: 3376 3462 3474 3820 1 Size: 86 12 346 449 Fragments arranged by size: 1012 802 467 449 346 335 281 246 209 86 23 12 BtgI C'CryG_G Cuts at: 107 794 107 Size: 687 3581 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2218 2399 2562 2218 Size: 181 163 3924 Fragments arranged by size: 3924 181 163 BtsI GCAGTG_nn' Cuts at: 1511 1511 Size: 4268 DraI TTT'AAA Cuts at: 1267 1267 Size: 4268 DrdI GACnn_nn'nnGTC Cuts at: 1974 2832 1974 Size: 858 3410 EaeI y'GGCC_r Cuts at: 235 1853 2027 2418 2445 3790 235 Size: 1618 174 391 27 1345 713 Fragments arranged by size: 1618 1345 713 391 174 27 EagI C'GGCC_G Cuts at: 1853 1853 Size: 4268 EarI CTCTTCn'nnn_ Cuts at: 119 517 1250 2291 2501 119 Size: 398 733 1041 210 1886 Fragments arranged by size: 1886 1041 733 398 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 983 2476 2786 2932 4078 983 Size: 1493 310 146 1146 1173 Fragments arranged by size: 1493 1173 1146 310 146 EcoICRI GAG'CTC Cuts at: 991 1280 991 Size: 289 3979 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 613 832 848 1131 2093 2525 3272 3540 613 Size: 219 16 283 962 432 747 268 1341 Fragments arranged by size: 1341 962 747 432 283 268 219 16 EcoNI CCTnn'n_nnAGG Cuts at: 1159 1183 1159 Size: 24 4244 EcoO109I rG'GnC_Cy Cuts at: 428 852 895 1407 428 Size: 424 43 512 3289 Fragments arranged by size: 3289 512 424 43 EcoRI G'AATT_C Cuts at: 1272 1272 Size: 4268 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1080 2287 1080 Size: 1207 3061 FspI TGC'GCA Cuts at: 2049 2049 Size: 4268 HaeII r_GCGC'y Cuts at: 648 808 1702 1950 2972 3951 648 Size: 160 894 248 1022 979 965 Fragments arranged by size: 1022 979 965 894 248 160 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 892 924 2110 2140 2142 2172 892 Size: 32 1186 30 2 30 2988 Fragments arranged by size: 2988 1186 32 30 30 2 HincII GTy'rAC Cuts at: 1307 1307 Size: 4268 HindIII A'AGCT_T Cuts at: 1085 1085 Size: 4268 KasI G'GCGC_C Cuts at: 804 1698 1946 804 Size: 894 248 3126 Fragments arranged by size: 3126 894 248 KpnI G_GTAC'C Cuts at: 1288 1288 Size: 4268 MluI A'CGCG_T Cuts at: 3701 3701 Size: 4268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2939 3123 2939 Size: 184 4084 MscI TGG'CCA Cuts at: 237 2029 237 Size: 1792 2476 MslI CAynn'nnrTG Cuts at: 568 764 2257 2384 568 Size: 196 1493 127 2452 Fragments arranged by size: 2452 1493 196 127 MspA1I CmG'CkG Cuts at: 315 399 1168 1379 1693 2053 3066 3311 315 Size: 84 769 211 314 360 1013 245 1272 Fragments arranged by size: 1272 1013 769 360 314 245 211 84 NaeI GCC'GGC Cuts at: 2449 2449 Size: 4268 NarI GG'CG_CC Cuts at: 805 1699 1947 805 Size: 894 248 3126 Fragments arranged by size: 3126 894 248 NcoI C'CATG_G Cuts at: 107 107 Size: 4268 NgoMIV G'CCGG_C Cuts at: 2447 2447 Size: 4268 NspI r_CATG'y Cuts at: 1321 1821 2728 1321 Size: 500 907 2861 Fragments arranged by size: 2861 907 500 PasI CC'CwG_GG Cuts at: 541 541 Size: 4268 PciI A'CATG_T Cuts at: 2724 2724 Size: 4268 PflMI CCAn_nnn'nTGG Cuts at: 865 865 Size: 4268 PfoI T'CCnGG_A Cuts at: 848 848 Size: 4268 PmeI GTTT'AAAC Cuts at: 1267 1267 Size: 4268 PmlI CAC'GTG Cuts at: 606 606 Size: 4268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3407 3439 3407 Size: 32 4236 PpuMI rG'GwC_Cy Cuts at: 852 852 Size: 4268 PspOMI G'GGCC_C Cuts at: 1049 1049 Size: 4268 PstI C_TGCA'G Cuts at: 1315 2000 1315 Size: 685 3583 PvuI CG_AT'CG Cuts at: 104 104 Size: 4268 PvuII CAG'CTG Cuts at: 399 1168 1693 2053 399 Size: 769 525 360 2614 Fragments arranged by size: 2614 769 525 360 RsrII CG'GwC_CG Cuts at: 2463 2463 Size: 4268 SacI G_AGCT'C Cuts at: 993 1282 993 Size: 289 3979 SalI G'TCGA_C Cuts at: 1305 1305 Size: 4268 SapI GCTCTTCn'nnn_ Cuts at: 119 1250 2291 2501 119 Size: 1131 1041 210 1886 Fragments arranged by size: 1886 1131 1041 210 SbfI CC_TGCA'GG Cuts at: 1315 1315 Size: 4268 ScaI AGT'ACT Cuts at: 3785 3785 Size: 4268 SfcI C'TryA_G Cuts at: 33 355 1311 1996 2989 3180 33 Size: 322 956 685 993 191 1121 Fragments arranged by size: 1121 993 956 685 322 191 SfoI GGC'GCC Cuts at: 806 1700 1948 806 Size: 894 248 3126 Fragments arranged by size: 3126 894 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4268 SmaI CCC'GGG Cuts at: 704 985 1290 704 Size: 281 305 3682 Fragments arranged by size: 3682 305 281 SmlI C'TyrA_G Cuts at: 1423 2338 2830 3092 1423 Size: 915 492 262 2599 Fragments arranged by size: 2599 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4268 SphI G_CATG'C Cuts at: 1321 1821 1321 Size: 500 3768 StyI C'CwwG_G Cuts at: 107 238 1402 1756 107 Size: 131 1164 354 2619 Fragments arranged by size: 2619 1164 354 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1851 1984 2443 2652 1851 Size: 133 459 209 3467 Fragments arranged by size: 3467 459 209 133 TatI w'GTAC_w Cuts at: 471 3783 471 Size: 3312 956 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 239 271 3785 3817 239 Size: 32 3514 32 690 Fragments arranged by size: 3514 690 32 32 Tth111I GACn'n_nGTC Cuts at: 2065 2065 Size: 4268 XbaI T'CTAG_A Cuts at: 1299 1299 Size: 4268 XcmI CCAnnnn_n'nnnnTGG Cuts at: 370 1763 370 Size: 1393 2875 XmaI C'CCGG_G Cuts at: 702 983 1288 702 Size: 281 305 3682 Fragments arranged by size: 3682 305 281 XmnI GAAnn'nnTTC Cuts at: 227 227 Size: 4268 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AleI AlfI AloI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI