Restriction map for
pF1KB7413
(Circular) (Six-base) MAPSORT of: pF1KB7413.seq Check: 8371 from: 1 to: 4268
pF1KB7413 4268 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 770 770
Size: 4268
AccI GT'mk_AC
Cuts at: 345 1306 345
Size: 961 3307
Acc65I G'GTAC_C
Cuts at: 1284 1284
Size: 4268
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1131 2093 2525 3272 3540 1131
Size: 962 432 747 268 1859
Fragments arranged by size:
1859 962 747 432 268
AflIII A'CryG_T
Cuts at: 1620 2724 3701 1620
Size: 1104 977 2187
Fragments arranged by size:
2187 1104 977
AgeI A'CCGG_T
Cuts at: 2656 2656
Size: 4268
AhdI GACnn_n'nnGTC
Cuts at: 906 906
Size: 4268
AlwNI CAG_nnn'CTG
Cuts at: 648 865 898 1171 1573 3140 648
Size: 217 33 273 402 1567 1776
Fragments arranged by size:
1776 1567 402 273 217 33
ApaI G_GGCC'C
Cuts at: 1053 1053
Size: 4268
ApaLI G'TGCA_C
Cuts at: 659 3038 659
Size: 2379 1889
ApoI r'AATT_y
Cuts at: 1272 3621 3877 1272
Size: 2349 256 1663
Fragments arranged by size:
2349 1663 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 702 983 1288 702
Size: 281 305 3682
Fragments arranged by size:
3682 305 281
BamHI G'GATC_C
Cuts at: 1293 1293
Size: 4268
BanI G'GyrC_C
Cuts at: 615 804 1118 1284 1698 1946 1981 615
Size: 189 314 166 414 248 35 2902
Fragments arranged by size:
2902 414 314 248 189 166 35
BanII G_rGCy'C
Cuts at: 818 993 1053 1282 2312 818
Size: 175 60 229 1030 2774
Fragments arranged by size:
2774 1030 229 175 60
BbeI G_GCGC'C
Cuts at: 808 1702 1950 808
Size: 894 248 3126
Fragments arranged by size:
3126 894 248
BbsI GAAGACnn'nnnn_
Cuts at: 566 1223 566
Size: 657 3611
BbvCI CC'TCA_GC
Cuts at: 1017 1017
Size: 4268
BciVI GTATCCnnnnn_n'
Cuts at: 2166 2927 4268 2166
Size: 761 1341 2166
Fragments arranged by size:
2166 1341 761
BclI T'GATC_A
Cuts at: 575 1788 575
Size: 1213 3055
BglI GCCn_nnn'nGGC
Cuts at: 734 734
Size: 4268
BglII A'GATC_T
Cuts at: 1783 3820 1783
Size: 2037 2231
BlpI GC'TnA_GC
Cuts at: 395 691 1380 395
Size: 296 689 3283
Fragments arranged by size:
3283 689 296
Bme1580I G_kGCm'C
Cuts at: 663 1053 1121 1893 1986 3042 663
Size: 390 68 772 93 1056 1889
Fragments arranged by size:
1889 1056 772 390 93 68
BmrI ACTGGGnnnn_n'
Cuts at: 177 1480 1561 1896 4040 177
Size: 1303 81 335 2144 405
Fragments arranged by size:
2144 1303 405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 526 2635 526
Size: 2109 2159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 832 848 613
Size: 219 16 4033
Fragments arranged by size:
4033 219 16
Bpu10I CC'TnA_GC
Cuts at: 620 1017 620
Size: 397 3871
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 2323 2815 3113 1444
Size: 879 492 298 2599
Fragments arranged by size:
2599 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3972 3972
Size: 4268
BsaAI yAC'GTr
Cuts at: 606 1621 606
Size: 1015 3253
BsaBI GATnn'nnATC
Cuts at: 6 1782 3819 6
Size: 1776 2037 455
Fragments arranged by size:
2037 1776 455
BsaHI Gr'CG_yC
Cuts at: 805 1699 1947 2649 4145 805
Size: 894 248 702 1496 928
Fragments arranged by size:
1496 928 894 702 248
BsaWI w'CCGG_w
Cuts at: 1457 1978 2656 2930 3077 3488 1457
Size: 521 678 274 147 411 2237
Fragments arranged by size:
2237 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 438 438
Size: 4268
BseYI C'CCAG_C
Cuts at: 324 641 858 1114 1693 2451 3028 3524 324
Size: 317 217 256 579 758 577 496 1068
Fragments arranged by size:
1068 758 579 577 496 317 256 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 439 275
Size: 164 4104
BsiEI CG_ry'CG
Cuts at: 104 1810 1856 3064 104
Size: 1706 46 1208 1308
Fragments arranged by size:
1706 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 663 993 1282 2060 2250 3042 663
Size: 330 289 778 190 792 1889
Fragments arranged by size:
1889 792 778 330 289 190
BsmI GAATG_Cn'
Cuts at: 249 249
Size: 4268
Bsp1286I G_dGCh'C
Cuts at: 663 818 993 1053 1121 1282 1893 1986 2060
Size: 155 175 60 68 161 611 93 74
Cuts at: 2060 2250 2312 3042 663
Size: 190 62 730 1889
Fragments arranged by size:
1889 730 611 190 175 161 155 93
74 68 62 60
BspEI T'CCGG_A
Cuts at: 3488 3488
Size: 4268
BspHI T'CATG_A
Cuts at: 3444 3444
Size: 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 770 1318 1532 1834 2215 770
Size: 548 214 302 381 2823
Fragments arranged by size:
2823 548 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2560 2614 4098 4263 50
Size: 2510 54 1484 165 55
Fragments arranged by size:
2510 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2180 2180
Size: 4268
BsrFI r'CCGG_y
Cuts at: 2266 2447 2656 2266
Size: 181 209 3878
Fragments arranged by size:
3878 209 181
BsrGI T'GTAC_A
Cuts at: 471 471
Size: 4268
BssSI C'ACGA_G
Cuts at: 884 2539 2897 884
Size: 1655 358 2255
Fragments arranged by size:
2255 1655 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1645 1645
Size: 4268
BstBI TT'CG_AA
Cuts at: 2629 2629
Size: 4268
BstXI CCAn_nnnn'nTGG
Cuts at: 766 766
Size: 4268
BstYI r'GATC_y
Cuts at: 1 210 491 1293 1760 1783 2118 2364 3376
Size: 209 281 802 467 23 335 246 1012
Cuts at: 3376 3462 3474 3820 1
Size: 86 12 346 449
Fragments arranged by size:
1012 802 467 449 346 335 281 246
209 86 23 12
BtgI C'CryG_G
Cuts at: 107 794 107
Size: 687 3581
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2218 2399 2562 2218
Size: 181 163 3924
Fragments arranged by size:
3924 181 163
BtsI GCAGTG_nn'
Cuts at: 1511 1511
Size: 4268
DraI TTT'AAA
Cuts at: 1267 1267
Size: 4268
DrdI GACnn_nn'nnGTC
Cuts at: 1974 2832 1974
Size: 858 3410
EaeI y'GGCC_r
Cuts at: 235 1853 2027 2418 2445 3790 235
Size: 1618 174 391 27 1345 713
Fragments arranged by size:
1618 1345 713 391 174 27
EagI C'GGCC_G
Cuts at: 1853 1853
Size: 4268
EarI CTCTTCn'nnn_
Cuts at: 119 517 1250 2291 2501 119
Size: 398 733 1041 210 1886
Fragments arranged by size:
1886 1041 733 398 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 983 2476 2786 2932 4078 983
Size: 1493 310 146 1146 1173
Fragments arranged by size:
1493 1173 1146 310 146
EcoICRI GAG'CTC
Cuts at: 991 1280 991
Size: 289 3979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 832 848 1131 2093 2525 3272 3540 613
Size: 219 16 283 962 432 747 268 1341
Fragments arranged by size:
1341 962 747 432 283 268 219 16
EcoNI CCTnn'n_nnAGG
Cuts at: 1159 1183 1159
Size: 24 4244
EcoO109I rG'GnC_Cy
Cuts at: 428 852 895 1407 428
Size: 424 43 512 3289
Fragments arranged by size:
3289 512 424 43
EcoRI G'AATT_C
Cuts at: 1272 1272
Size: 4268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1080 2287 1080
Size: 1207 3061
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 648 808 1702 1950 2972 3951 648
Size: 160 894 248 1022 979 965
Fragments arranged by size:
1022 979 965 894 248 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 892 924 2110 2140 2142 2172 892
Size: 32 1186 30 2 30 2988
Fragments arranged by size:
2988 1186 32 30 30 2
HincII GTy'rAC
Cuts at: 1307 1307
Size: 4268
HindIII A'AGCT_T
Cuts at: 1085 1085
Size: 4268
KasI G'GCGC_C
Cuts at: 804 1698 1946 804
Size: 894 248 3126
Fragments arranged by size:
3126 894 248
KpnI G_GTAC'C
Cuts at: 1288 1288
Size: 4268
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2939 3123 2939
Size: 184 4084
MscI TGG'CCA
Cuts at: 237 2029 237
Size: 1792 2476
MslI CAynn'nnrTG
Cuts at: 568 764 2257 2384 568
Size: 196 1493 127 2452
Fragments arranged by size:
2452 1493 196 127
MspA1I CmG'CkG
Cuts at: 315 399 1168 1379 1693 2053 3066 3311 315
Size: 84 769 211 314 360 1013 245 1272
Fragments arranged by size:
1272 1013 769 360 314 245 211 84
NaeI GCC'GGC
Cuts at: 2449 2449
Size: 4268
NarI GG'CG_CC
Cuts at: 805 1699 1947 805
Size: 894 248 3126
Fragments arranged by size:
3126 894 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4268
NgoMIV G'CCGG_C
Cuts at: 2447 2447
Size: 4268
NspI r_CATG'y
Cuts at: 1321 1821 2728 1321
Size: 500 907 2861
Fragments arranged by size:
2861 907 500
PasI CC'CwG_GG
Cuts at: 541 541
Size: 4268
PciI A'CATG_T
Cuts at: 2724 2724
Size: 4268
PflMI CCAn_nnn'nTGG
Cuts at: 865 865
Size: 4268
PfoI T'CCnGG_A
Cuts at: 848 848
Size: 4268
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PmlI CAC'GTG
Cuts at: 606 606
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 4236
PpuMI rG'GwC_Cy
Cuts at: 852 852
Size: 4268
PspOMI G'GGCC_C
Cuts at: 1049 1049
Size: 4268
PstI C_TGCA'G
Cuts at: 1315 2000 1315
Size: 685 3583
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 399 1168 1693 2053 399
Size: 769 525 360 2614
Fragments arranged by size:
2614 769 525 360
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 993 1282 993
Size: 289 3979
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 119 1250 2291 2501 119
Size: 1131 1041 210 1886
Fragments arranged by size:
1886 1131 1041 210
SbfI CC_TGCA'GG
Cuts at: 1315 1315
Size: 4268
ScaI AGT'ACT
Cuts at: 3785 3785
Size: 4268
SfcI C'TryA_G
Cuts at: 33 355 1311 1996 2989 3180 33
Size: 322 956 685 993 191 1121
Fragments arranged by size:
1121 993 956 685 322 191
SfoI GGC'GCC
Cuts at: 806 1700 1948 806
Size: 894 248 3126
Fragments arranged by size:
3126 894 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 704 985 1290 704
Size: 281 305 3682
Fragments arranged by size:
3682 305 281
SmlI C'TyrA_G
Cuts at: 1423 2338 2830 3092 1423
Size: 915 492 262 2599
Fragments arranged by size:
2599 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4268
SphI G_CATG'C
Cuts at: 1321 1821 1321
Size: 500 3768
StyI C'CwwG_G
Cuts at: 107 238 1402 1756 107
Size: 131 1164 354 2619
Fragments arranged by size:
2619 1164 354 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1851 1984 2443 2652 1851
Size: 133 459 209 3467
Fragments arranged by size:
3467 459 209 133
TatI w'GTAC_w
Cuts at: 471 3783 471
Size: 3312 956
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 239 271 3785 3817 239
Size: 32 3514 32 690
Fragments arranged by size:
3514 690 32 32
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 1299 1299
Size: 4268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 370 1763 370
Size: 1393 2875
XmaI C'CCGG_G
Cuts at: 702 983 1288 702
Size: 281 305 3682
Fragments arranged by size:
3682 305 281
XmnI GAAnn'nnTTC
Cuts at: 227 227
Size: 4268
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI