Restriction map for
pF1KB7414
(Circular) (Six-base) MAPSORT of: pF1KB7414.seq Check: 4025 from: 1 to: 4379
pF1KB7414 4379 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1417 1417
Size: 4379
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4379
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 789 957 1293 2204 2636 3383 3651 426
Size: 363 168 336 911 432 747 268 1154
Fragments arranged by size:
1154 911 747 432 363 336 268 168
AflIII A'CryG_T
Cuts at: 1731 2835 3812 1731
Size: 1104 977 2298
Fragments arranged by size:
2298 1104 977
AgeI A'CCGG_T
Cuts at: 2767 2767
Size: 4379
AleI CACnn'nnGTG
Cuts at: 764 764
Size: 4379
AlwNI CAG_nnn'CTG
Cuts at: 1684 3251 1684
Size: 1567 2812
ApaI G_GGCC'C
Cuts at: 210 210
Size: 4379
ApaLI G'TGCA_C
Cuts at: 3149 3149
Size: 4379
ApoI r'AATT_y
Cuts at: 1175 1383 3732 3988 1175
Size: 208 2349 256 1566
Fragments arranged by size:
2349 1566 256 208
AseI AT'TA_AT
Cuts at: 20 20
Size: 4379
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 1399 1399
Size: 4379
BamHI G'GATC_C
Cuts at: 1404 1404
Size: 4379
BanI G'GyrC_C
Cuts at: 125 578 1395 1809 2057 2092 125
Size: 453 817 414 248 35 2412
Fragments arranged by size:
2412 817 453 414 248 35
BanII G_rGCy'C
Cuts at: 210 1393 2423 210
Size: 1183 1030 2166
Fragments arranged by size:
2166 1183 1030
BbeI G_GCGC'C
Cuts at: 129 1813 2061 129
Size: 1684 248 2447
Fragments arranged by size:
2447 1684 248
BbsI GAAGACnn'nnnn_
Cuts at: 981 1363 981
Size: 382 3997
BbvCI CC'TCA_GC
Cuts at: 570 570
Size: 4379
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3038 4379 2277
Size: 761 1341 2277
Fragments arranged by size:
2277 1341 761
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BglII A'GATC_T
Cuts at: 1894 3931 1894
Size: 2037 2342
BlpI GC'TnA_GC
Cuts at: 1491 1491
Size: 4379
Bme1580I G_kGCm'C
Cuts at: 210 2004 2097 3153 210
Size: 1794 93 1056 1436
Fragments arranged by size:
1794 1436 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1025 1109 1193 1591 1672 2007 4151 1025
Size: 84 84 398 81 335 2144 1253
Fragments arranged by size:
2144 1253 398 335 84 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 316 953 979 250
Size: 66 637 26 3650
Fragments arranged by size:
3650 637 66 26
Bpu10I CC'TnA_GC
Cuts at: 570 570
Size: 4379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1053 1555 2434 2926 3224 1053
Size: 502 879 492 298 2208
Fragments arranged by size:
2208 879 502 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 291 4083 291
Size: 3792 587
BsaAI yAC'GTr
Cuts at: 1732 1732
Size: 4379
BsaBI GATnn'nnATC
Cuts at: 6 1893 3930 6
Size: 1887 2037 455
Fragments arranged by size:
2037 1887 455
BsaHI Gr'CG_yC
Cuts at: 126 1810 2058 2760 4256 126
Size: 1684 248 702 1496 249
Fragments arranged by size:
1684 1496 702 249 248
BsaWI w'CCGG_w
Cuts at: 1568 2089 2767 3041 3188 3599 1568
Size: 521 678 274 147 411 2348
Fragments arranged by size:
2348 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 564 564
Size: 4379
BseYI C'CCAG_C
Cuts at: 290 479 1804 2562 3139 3635 290
Size: 189 1325 758 577 496 1034
Fragments arranged by size:
1325 1034 758 577 496 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 220
Size: 4379
BsiEI CG_ry'CG
Cuts at: 104 1921 1967 3175 104
Size: 1817 46 1208 1308
Fragments arranged by size:
1817 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1393 2171 2361 3153 1393
Size: 778 190 792 2619
Fragments arranged by size:
2619 792 778 190
BsmI GAATG_Cn'
Cuts at: 259 787 1097 1222 259
Size: 528 310 125 3416
Fragments arranged by size:
3416 528 310 125
Bsp1286I G_dGCh'C
Cuts at: 210 1393 2004 2097 2171 2361 2423 3153 210
Size: 1183 611 93 74 190 62 730 1436
Fragments arranged by size:
1436 1183 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3599 3599
Size: 4379
BspHI T'CATG_A
Cuts at: 3555 3555
Size: 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1429 1643 1945 2326 1429
Size: 214 302 381 3482
Fragments arranged by size:
3482 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4209 4374 50
Size: 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2291 2291
Size: 4379
BsrFI r'CCGG_y
Cuts at: 2377 2558 2767 2377
Size: 181 209 3989
Fragments arranged by size:
3989 209 181
BsrGI T'GTAC_A
Cuts at: 212 212
Size: 4379
BssSI C'ACGA_G
Cuts at: 2650 3008 2650
Size: 358 4021
BstAPI GCAn_nnn'nTGC
Cuts at: 1756 1756
Size: 4379
BstBI TT'CG_AA
Cuts at: 2740 2740
Size: 4379
BstYI r'GATC_y
Cuts at: 1 1404 1871 1894 2229 2475 3487 3573 3585
Size: 1403 467 23 335 246 1012 86 12
Cuts at: 3585 3931 1
Size: 346 449
Fragments arranged by size:
1403 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 313 313
Size: 4379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2329 2510 2673 2329
Size: 181 163 4035
Fragments arranged by size:
4035 181 163
BtsI GCAGTG_nn'
Cuts at: 1622 1622
Size: 4379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 774 809 774
Size: 35 4344
DraI TTT'AAA
Cuts at: 864 1378 864
Size: 514 3865
DrdI GACnn_nn'nnGTC
Cuts at: 2085 2943 2085
Size: 858 3521
EaeI y'GGCC_r
Cuts at: 1964 2138 2529 2556 3901 1964
Size: 174 391 27 1345 2442
Fragments arranged by size:
2442 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4379
EarI CTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 2897 3043 4189 2587
Size: 310 146 1146 2777
Fragments arranged by size:
2777 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 316 426 789 953 957 979 1293 2204
Size: 66 110 363 164 4 22 314 911
Cuts at: 2204 2636 3383 3651 250
Size: 432 747 268 978
Fragments arranged by size:
978 911 747 432 363 314 268 164
110 66 22 4
EcoNI CCTnn'n_nnAGG
Cuts at: 573 573
Size: 4379
EcoO109I rG'GnC_Cy
Cuts at: 206 207 318 459 1518 206
Size: 1 111 141 1059 3067
Fragments arranged by size:
3067 1059 141 111 1
EcoRI G'AATT_C
Cuts at: 1175 1383 1175
Size: 208 4171
EcoRV GAT'ATC
Cuts at: 689 689
Size: 4379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 129 1813 2061 3083 4062 129
Size: 1684 248 1022 979 446
Fragments arranged by size:
1684 1022 979 446 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 900 932 1152 1184 2221 2251 2253
Size: 32 462 32 220 32 1037 30 2
Cuts at: 2253 2283 406
Size: 30 2502
Fragments arranged by size:
2502 1037 462 220 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1418 1418
Size: 4379
KasI G'GCGC_C
Cuts at: 125 1809 2057 125
Size: 1684 248 2447
Fragments arranged by size:
2447 1684 248
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4379
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1172 3050 3234 1027
Size: 145 1878 184 2172
Fragments arranged by size:
2172 1878 184 145
MscI TGG'CCA
Cuts at: 2140 2140
Size: 4379
MslI CAynn'nnrTG
Cuts at: 727 746 764 864 2368 2495 727
Size: 19 18 100 1504 127 2611
Fragments arranged by size:
2611 1504 127 100 19 18
MspA1I CmG'CkG
Cuts at: 294 1313 1490 1804 2164 3177 3422 294
Size: 1019 177 314 360 1013 245 1251
Fragments arranged by size:
1251 1019 1013 360 314 245 177
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4379
NarI GG'CG_CC
Cuts at: 126 1810 2058 126
Size: 1684 248 2447
Fragments arranged by size:
2447 1684 248
NcoI C'CATG_G
Cuts at: 313 313
Size: 4379
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4379
NspI r_CATG'y
Cuts at: 1432 1932 2839 1432
Size: 500 907 2972
Fragments arranged by size:
2972 907 500
PasI CC'CwG_GG
Cuts at: 329 329
Size: 4379
PciI A'CATG_T
Cuts at: 2835 2835
Size: 4379
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 4347
PshAI GACnn'nnGTC
Cuts at: 1363 1363
Size: 4379
PspOMI G'GGCC_C
Cuts at: 206 206
Size: 4379
PstI C_TGCA'G
Cuts at: 1426 2111 1426
Size: 685 3694
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 294 1313 1804 2164 294
Size: 1019 491 360 2509
Fragments arranged by size:
2509 1019 491 360
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4379
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 3896 3896
Size: 4379
SfcI C'TryA_G
Cuts at: 33 1422 2107 3100 3291 33
Size: 1389 685 993 191 1121
Fragments arranged by size:
1389 1121 993 685 191
SfoI GGC'GCC
Cuts at: 127 1811 2059 127
Size: 1684 248 2447
Fragments arranged by size:
2447 1684 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 1068 1534 2449 2941 3203 1068
Size: 466 915 492 262 2244
Fragments arranged by size:
2244 915 492 466 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3879
SspI AAT'ATT
Cuts at: 636 636
Size: 4379
StuI AGG'CCT
Cuts at: 589 970 589
Size: 381 3998
StyI C'CwwG_G
Cuts at: 313 1513 1867 313
Size: 1200 354 2825
Fragments arranged by size:
2825 1200 354
SwaI ATTT'AAAT
Cuts at: 864 864
Size: 4379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 184 1962 2095 2554 2763 184
Size: 1778 133 459 209 1800
Fragments arranged by size:
1800 1778 459 209 133
TatI w'GTAC_w
Cuts at: 161 212 3894 161
Size: 51 3682 646
Fragments arranged by size:
3682 646 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4347
Tth111I GACn'n_nGTC
Cuts at: 540 2176 540
Size: 1636 2743
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 279 521 1874 279
Size: 242 1353 2784
Fragments arranged by size:
2784 1353 242
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
XmnI GAAnn'nnTTC
Cuts at: 1054 1222 1054
Size: 168 4211
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI XhoI ZraI