Restriction map for
pF1KB7415
(Circular) (Six-base) MAPSORT of: pF1KB7415.seq Check: 9674 from: 1 to: 4076
pF1KB7415 4076 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 1092 1092
Size: 4076
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 1901 2333 3080 3348 210
Size: 1691 432 747 268 938
Fragments arranged by size:
1691 938 747 432 268
AflIII A'CryG_T
Cuts at: 1428 2532 3509 1428
Size: 1104 977 1995
Fragments arranged by size:
1995 1104 977
AgeI A'CCGG_T
Cuts at: 2464 2464
Size: 4076
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 511 543 511
Size: 32 4044
AlwNI CAG_nnn'CTG
Cuts at: 277 1381 2948 277
Size: 1104 1567 1405
Fragments arranged by size:
1567 1405 1104
ApaLI G'TGCA_C
Cuts at: 2846 2846
Size: 4076
ApoI r'AATT_y
Cuts at: 285 354 380 401 1080 3429 3685 285
Size: 69 26 21 679 2349 256 676
Fragments arranged by size:
2349 679 676 256 69 26 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 772 1096 772
Size: 324 3752
BamHI G'GATC_C
Cuts at: 1101 1101
Size: 4076
BanI G'GyrC_C
Cuts at: 1092 1506 1754 1789 1092
Size: 414 248 35 3379
Fragments arranged by size:
3379 414 248 35
BanII G_rGCy'C
Cuts at: 924 1090 2120 924
Size: 166 1030 2880
Fragments arranged by size:
2880 1030 166
BbeI G_GCGC'C
Cuts at: 1510 1758 1510
Size: 248 3828
BbsI GAAGACnn'nnnn_
Cuts at: 678 678
Size: 4076
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 1596 1596
Size: 4076
BfrBI ATG'CAT
Cuts at: 496 496
Size: 4076
BglII A'GATC_T
Cuts at: 1591 3628 1591
Size: 2037 2039
BlpI GC'TnA_GC
Cuts at: 1188 1188
Size: 4076
Bme1580I G_kGCm'C
Cuts at: 622 719 1701 1794 2850 622
Size: 97 982 93 1056 1848
Fragments arranged by size:
1848 1056 982 97 93
BmrI ACTGGGnnnn_n'
Cuts at: 1288 1369 1704 3848 1288
Size: 81 335 2144 1516
Fragments arranged by size:
2144 1516 335 81
BmtI G_CTAG'C
Cuts at: 869 869
Size: 4076
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 236
Size: 4076
Bpu10I CC'TnA_GC
Cuts at: 263 263
Size: 4076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1252 2131 2623 2921 502
Size: 750 879 492 298 1657
Fragments arranged by size:
1657 879 750 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 218 413 3780 218
Size: 195 3367 514
Fragments arranged by size:
3367 514 195
BsaAI yAC'GTr
Cuts at: 1429 1429
Size: 4076
BsaBI GATnn'nnATC
Cuts at: 6 1590 3627 6
Size: 1584 2037 455
Fragments arranged by size:
2037 1584 455
BsaHI Gr'CG_yC
Cuts at: 1507 1755 2457 3953 1507
Size: 248 702 1496 1630
Fragments arranged by size:
1630 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1265 1786 2464 2738 2885 3296 1265
Size: 521 678 274 147 411 2045
Fragments arranged by size:
2045 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 511 541 511
Size: 30 4046
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 499
Size: 4076
BseYI C'CCAG_C
Cuts at: 621 1501 2259 2836 3332 621
Size: 880 758 577 496 1365
Fragments arranged by size:
1365 880 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1618 1664 2872 104
Size: 1514 46 1208 1308
Fragments arranged by size:
1514 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 924 1090 1868 2058 2850 924
Size: 166 778 190 792 2150
Fragments arranged by size:
2150 792 778 190 166
BsmI GAATG_Cn'
Cuts at: 496 496
Size: 4076
BsmBI CGTCTCn'nnnn_
Cuts at: 122 122
Size: 4076
Bsp1286I G_dGCh'C
Cuts at: 622 719 924 1090 1701 1794 1868 2058 2120
Size: 97 205 166 611 93 74 190 62
Cuts at: 2120 2850 622
Size: 730 1848
Fragments arranged by size:
1848 730 611 205 190 166 97 93
74 62
BspEI T'CCGG_A
Cuts at: 3296 3296
Size: 4076
BspHI T'CATG_A
Cuts at: 216 3252 216
Size: 3036 1040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1126 1340 1642 2023 1126
Size: 214 302 381 3179
Fragments arranged by size:
3179 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2368 2422 3906 4071 50
Size: 2318 54 1484 165 55
Fragments arranged by size:
2318 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1988 1988
Size: 4076
BsrFI r'CCGG_y
Cuts at: 2074 2255 2464 2074
Size: 181 209 3686
Fragments arranged by size:
3686 209 181
BssSI C'ACGA_G
Cuts at: 2347 2705 2347
Size: 358 3718
BstAPI GCAn_nnn'nTGC
Cuts at: 741 1453 741
Size: 712 3364
BstBI TT'CG_AA
Cuts at: 399 2437 399
Size: 2038 2038
BstXI CCAn_nnnn'nTGG
Cuts at: 550 752 550
Size: 202 3874
BstYI r'GATC_y
Cuts at: 1 1101 1568 1591 1926 2172 3184 3270 3282
Size: 1100 467 23 335 246 1012 86 12
Cuts at: 3282 3628 1
Size: 346 449
Fragments arranged by size:
1100 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4076
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 946 986 1319 946
Size: 40 333 3703
Fragments arranged by size:
3703 333 40
DraI TTT'AAA
Cuts at: 650 1075 650
Size: 425 3651
DrdI GACnn_nn'nnGTC
Cuts at: 1782 2640 1782
Size: 858 3218
EaeI y'GGCC_r
Cuts at: 311 363 1661 1835 2226 2253 3598 311
Size: 52 1298 174 391 27 1345 789
Fragments arranged by size:
1345 1298 789 391 174 52 27
EagI C'GGCC_G
Cuts at: 1661 1661
Size: 4076
EarI CTCTTCn'nnn_
Cuts at: 929 2099 2309 929
Size: 1170 210 2696
Fragments arranged by size:
2696 1170 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2284 2594 2740 3886 2284
Size: 310 146 1146 2474
Fragments arranged by size:
2474 1146 310 146
EcoICRI GAG'CTC
Cuts at: 922 1088 922
Size: 166 3910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 236 1901 2333 3080 3348 210
Size: 26 1665 432 747 268 938
Fragments arranged by size:
1665 938 747 432 268 26
EcoO109I rG'GnC_Cy
Cuts at: 259 1215 259
Size: 956 3120
EcoRI G'AATT_C
Cuts at: 380 1080 380
Size: 700 3376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 4076
FspI TGC'GCA
Cuts at: 1857 1857
Size: 4076
HaeII r_GCGC'y
Cuts at: 1510 1758 2780 3759 1510
Size: 248 1022 979 1827
Fragments arranged by size:
1827 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 144 176 232 264 893 925 1918 1948 1950
Size: 32 56 32 629 32 993 30 2
Cuts at: 1950 1980 144
Size: 30 2240
Fragments arranged by size:
2240 993 629 56 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1115 1115
Size: 4076
KasI G'GCGC_C
Cuts at: 1506 1754 1506
Size: 248 3828
KpnI G_GTAC'C
Cuts at: 1096 1096
Size: 4076
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2747 2931 2747
Size: 184 3892
MscI TGG'CCA
Cuts at: 313 365 1837 313
Size: 52 1472 2552
Fragments arranged by size:
2552 1472 52
MslI CAynn'nnrTG
Cuts at: 548 2065 2192 548
Size: 1517 127 2432
Fragments arranged by size:
2432 1517 127
MspA1I CmG'CkG
Cuts at: 1187 1501 1861 2874 3119 1187
Size: 314 360 1013 245 2144
Fragments arranged by size:
2144 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 4076
NarI GG'CG_CC
Cuts at: 1507 1755 1507
Size: 248 3828
NcoI C'CATG_G
Cuts at: 107 107
Size: 4076
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 4076
NheI G'CTAG_C
Cuts at: 865 865
Size: 4076
NsiI A_TGCA'T
Cuts at: 498 498
Size: 4076
NspI r_CATG'y
Cuts at: 177 1129 1629 2536 177
Size: 952 500 907 1717
Fragments arranged by size:
1717 952 907 500
PasI CC'CwG_GG
Cuts at: 752 752
Size: 4076
PciI A'CATG_T
Cuts at: 2532 2532
Size: 4076
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 511 543 3215 3247 511
Size: 32 2672 32 1340
Fragments arranged by size:
2672 1340 32 32
PpuMI rG'GwC_Cy
Cuts at: 259 259
Size: 4076
PstI C_TGCA'G
Cuts at: 1123 1808 1123
Size: 685 3391
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 1501 1861 1501
Size: 360 3716
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 924 1090 924
Size: 166 3910
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 929 2099 2309 929
Size: 1170 210 2696
Fragments arranged by size:
2696 1170 210
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SfcI C'TryA_G
Cuts at: 33 1119 1804 2797 2988 33
Size: 1086 685 993 191 1121
Fragments arranged by size:
1121 1086 993 685 191
SfoI GGC'GCC
Cuts at: 1508 1756 1508
Size: 248 3828
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 1098 1098
Size: 4076
SmlI C'TyrA_G
Cuts at: 481 1231 2146 2638 2900 481
Size: 750 915 492 262 1657
Fragments arranged by size:
1657 915 750 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 177 1129 1629 177
Size: 952 500 2624
Fragments arranged by size:
2624 952 500
SspI AAT'ATT
Cuts at: 981 981
Size: 4076
StuI AGG'CCT
Cuts at: 479 479
Size: 4076
StyI C'CwwG_G
Cuts at: 107 1210 1564 107
Size: 1103 354 2619
Fragments arranged by size:
2619 1103 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1659 1792 2251 2460 1659
Size: 133 459 209 3275
Fragments arranged by size:
3275 459 209 133
TatI w'GTAC_w
Cuts at: 3591 3591
Size: 4076
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4044
Tth111I GACn'n_nGTC
Cuts at: 1873 1873
Size: 4076
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 373 1571 373
Size: 1198 2878
XmaI C'CCGG_G
Cuts at: 1096 1096
Size: 4076
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PasI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BsgI
BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI PacI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI