Restriction map for
pF1KB7416
(Circular) (Six-base) MAPSORT of: pF1KB7416.seq Check: 660 from: 1 to: 4514
pF1KB7416 4514 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AatII G_ACGT'C
Cuts at: 278 278
Size: 4514
AccI GT'mk_AC
Cuts at: 1552 1552
Size: 4514
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 789 996 1354 2339 2771 3518 3786 789
Size: 207 358 985 432 747 268 1517
Fragments arranged by size:
1517 985 747 432 358 268 207
AfeI AGC'GCT
Cuts at: 815 815
Size: 4514
AflIII A'CryG_T
Cuts at: 1343 1395 1866 2970 3947 1343
Size: 52 471 1104 977 1910
Fragments arranged by size:
1910 1104 977 471 52
AgeI A'CCGG_T
Cuts at: 2902 2902
Size: 4514
AleI CACnn'nnGTG
Cuts at: 1156 1459 1156
Size: 303 4211
AlwNI CAG_nnn'CTG
Cuts at: 234 1103 1182 1239 1369 1819 3386 234
Size: 869 79 57 130 450 1567 1362
Fragments arranged by size:
1567 1362 869 450 130 79 57
ApaI G_GGCC'C
Cuts at: 705 1053 705
Size: 348 4166
ApaLI G'TGCA_C
Cuts at: 3284 3284
Size: 4514
ApoI r'AATT_y
Cuts at: 1518 3867 4123 1518
Size: 2349 256 1909
Fragments arranged by size:
2349 1909 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4514
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 143 697 827 1428 1534 143
Size: 554 130 601 106 3123
Fragments arranged by size:
3123 601 554 130 106
BamHI G'GATC_C
Cuts at: 736 1373 1539 736
Size: 637 166 3711
Fragments arranged by size:
3711 637 166
BanI G'GyrC_C
Cuts at: 334 908 1530 1944 2192 2227 334
Size: 574 622 414 248 35 2621
Fragments arranged by size:
2621 622 574 414 248 35
BanII G_rGCy'C
Cuts at: 705 1028 1053 1435 1469 1528 2558 705
Size: 323 25 382 34 59 1030 2661
Fragments arranged by size:
2661 1030 382 323 59 34 25
BbeI G_GCGC'C
Cuts at: 338 1948 2196 338
Size: 1610 248 2656
Fragments arranged by size:
2656 1610 248
BbvCI CC'TCA_GC
Cuts at: 610 1096 610
Size: 486 4028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 258 292 258
Size: 34 4480
BciVI GTATCCnnnnn_n'
Cuts at: 2412 3173 4514 2412
Size: 761 1341 2412
Fragments arranged by size:
2412 1341 761
BclI T'GATC_A
Cuts at: 2034 2034
Size: 4514
BglI GCCn_nnn'nGGC
Cuts at: 1360 1360
Size: 4514
BglII A'GATC_T
Cuts at: 2029 4066 2029
Size: 2037 2477
BlpI GC'TnA_GC
Cuts at: 1626 1626
Size: 4514
Bme1580I G_kGCm'C
Cuts at: 705 1053 2139 2232 3288 705
Size: 348 1086 93 1056 1931
Fragments arranged by size:
1931 1086 1056 348 93
BmrI ACTGGGnnnn_n'
Cuts at: 960 1212 1726 1807 2142 4286 960
Size: 252 514 81 335 2144 1188
Fragments arranged by size:
2144 1188 514 335 252 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1111 2881 1111
Size: 1770 2744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1051 1261 1402 736
Size: 315 210 141 3848
Fragments arranged by size:
3848 315 210 141
Bpu10I CC'TnA_GC
Cuts at: 610 1096 610
Size: 486 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1690 2569 3061 3359 871
Size: 819 879 492 298 2026
Fragments arranged by size:
2026 879 819 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4218 4218
Size: 4514
BsaAI yAC'GTr
Cuts at: 1412 1867 1412
Size: 455 4059
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 275 335 409 944 1280 1945 2193 2895 4391
Size: 60 74 535 336 665 248 702 1496
Cuts at: 4391 275
Size: 398
Fragments arranged by size:
1496 702 665 535 398 336 248 74
60
BsaWI w'CCGG_w
Cuts at: 1703 2224 2902 3176 3323 3734 1703
Size: 521 678 274 147 411 2483
Fragments arranged by size:
2483 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 784 814 1487 1517 784
Size: 30 673 30 3781
Fragments arranged by size:
3781 673 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 328 331 1132 328
Size: 3 801 3710
Fragments arranged by size:
3710 801 3
BseYI C'CCAG_C
Cuts at: 816 916 1283 1369 1435 1939 2697 3274 3770
Size: 100 367 86 66 504 758 577 496
Cuts at: 3770 816
Size: 1560
Fragments arranged by size:
1560 758 577 504 496 367 100 86
66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 310 367 794 1273 1427 301
Size: 9 57 427 479 154 3388
Fragments arranged by size:
3388 479 427 154 57 9
BsiEI CG_ry'CG
Cuts at: 104 138 2056 2102 3310 104
Size: 34 1918 46 1208 1308
Fragments arranged by size:
1918 1308 1208 46 34
BsiHKAI G_wGCw'C
Cuts at: 761 1028 1528 2306 2496 3288 761
Size: 267 500 778 190 792 1987
Fragments arranged by size:
1987 792 778 500 267 190
BsmI GAATG_Cn'
Cuts at: 966 966
Size: 4514
BsmBI CGTCTCn'nnnn_
Cuts at: 312 400 1386 312
Size: 88 986 3440
Fragments arranged by size:
3440 986 88
Bsp1286I G_dGCh'C
Cuts at: 705 761 1028 1053 1435 1469 1528 2139 2232
Size: 56 267 25 382 34 59 611 93
Cuts at: 2232 2306 2496 2558 3288 705
Size: 74 190 62 730 1931
Fragments arranged by size:
1931 730 611 382 267 190 93 74
62 59 56 34 25
BspEI T'CCGG_A
Cuts at: 3734 3734
Size: 4514
BspHI T'CATG_A
Cuts at: 761 956 1304 3690 761
Size: 195 348 2386 1585
Fragments arranged by size:
2386 1585 348 195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1564 1778 2080 2461 1564
Size: 214 302 381 3617
Fragments arranged by size:
3617 381 302 214
BsrBI CCG'CTC
Cuts at: 50 177 2806 2860 4344 4509 50
Size: 127 2629 54 1484 165 55
Fragments arranged by size:
2629 1484 165 127 55 54
BsrDI GCAATG_nn'
Cuts at: 650 2426 650
Size: 1776 2738
BsrFI r'CCGG_y
Cuts at: 115 456 548 2512 2693 2902 115
Size: 341 92 1964 181 209 1727
Fragments arranged by size:
1964 1727 341 209 181 92
BsrGI T'GTAC_A
Cuts at: 1346 1346
Size: 4514
BssSI C'ACGA_G
Cuts at: 898 2785 3143 898
Size: 1887 358 2269
Fragments arranged by size:
2269 1887 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1891 1891
Size: 4514
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstEII G'GTnAC_C
Cuts at: 240 240
Size: 4514
BstXI CCAn_nnnn'nTGG
Cuts at: 486 1158 1461 486
Size: 672 303 3539
Fragments arranged by size:
3539 672 303
BstYI r'GATC_y
Cuts at: 1 736 929 1373 1539 2006 2029 2364 2610
Size: 735 193 444 166 467 23 335 246
Cuts at: 2610 3622 3708 3720 4066 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 735 467 449 444 346 335 246
193 166 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 451 451
Size: 4514
BtgI C'CryG_G
Cuts at: 107 968 1040 1226 1420 107
Size: 861 72 186 194 3201
Fragments arranged by size:
3201 861 194 186 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2464 2645 2808 2464
Size: 181 163 4170
Fragments arranged by size:
4170 181 163
BtsI GCAGTG_nn'
Cuts at: 289 1757 289
Size: 1468 3046
DraI TTT'AAA
Cuts at: 1513 1513
Size: 4514
DraIII CAC_nnn'GTG
Cuts at: 261 261
Size: 4514
DrdI GACnn_nn'nnGTC
Cuts at: 646 2220 3078 646
Size: 1574 858 2082
Fragments arranged by size:
2082 1574 858
EaeI y'GGCC_r
Cuts at: 110 135 397 488 873 1505 2099 2273 2664
Size: 25 262 91 385 632 594 174 391
Cuts at: 2664 2691 4036 110
Size: 27 1345 588
Fragments arranged by size:
1345 632 594 588 391 385 262 174
91 27 25
EagI C'GGCC_G
Cuts at: 135 2099 135
Size: 1964 2550
EarI CTCTTCn'nnn_
Cuts at: 1384 2537 2747 1384
Size: 1153 210 3151
Fragments arranged by size:
3151 1153 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2722 3032 3178 4324 2722
Size: 310 146 1146 2912
Fragments arranged by size:
2912 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1026 1526 1026
Size: 500 4014
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 789 996 1051 1261 1354 1402 2339 2771
Size: 53 207 55 210 93 48 937 432
Cuts at: 2771 3518 3786 736
Size: 747 268 1464
Fragments arranged by size:
1464 937 747 432 268 210 207 93
55 53 48
EcoNI CCTnn'n_nnAGG
Cuts at: 1323 1363 1323
Size: 40 4474
EcoO109I rG'GnC_Cy
Cuts at: 198 321 515 579 689 701 702 824 1035
Size: 123 194 64 110 12 1 122 211
Cuts at: 1035 1146 1353 1653 198
Size: 111 207 300 3059
Fragments arranged by size:
3059 300 211 207 194 123 122 111
110 64 12 1
EcoRI G'AATT_C
Cuts at: 1518 1518
Size: 4514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4514
FspI TGC'GCA
Cuts at: 2295 2295
Size: 4514
HaeII r_GCGC'y
Cuts at: 234 338 817 1948 2196 3218 4197 234
Size: 104 479 1131 248 1022 979 551
Fragments arranged by size:
1131 1022 979 551 479 248 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 144 176 2356 2386 2388 2418 144
Size: 32 2180 30 2 30 2240
Fragments arranged by size:
2240 2180 32 30 30 2
HincII GTy'rAC
Cuts at: 1209 1553 1209
Size: 344 4170
KasI G'GCGC_C
Cuts at: 334 1944 2192 334
Size: 1610 248 2656
Fragments arranged by size:
2656 1610 248
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MluI A'CGCG_T
Cuts at: 1395 3947 1395
Size: 2552 1962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3185 3369 3185
Size: 184 4330
MscI TGG'CCA
Cuts at: 490 2275 490
Size: 1785 2729
MslI CAynn'nnrTG
Cuts at: 1156 1265 1297 1303 1459 2503 2630 1156
Size: 109 32 6 156 1044 127 3040
Fragments arranged by size:
3040 1044 156 127 109 32 6
MspA1I CmG'CkG
Cuts at: 369 609 1287 1380 1474 1625 1939 2299 3312
Size: 240 678 93 94 151 314 360 1013
Cuts at: 3312 3557 369
Size: 245 1326
Fragments arranged by size:
1326 1013 678 360 314 245 240 151
94 93
NaeI GCC'GGC
Cuts at: 117 458 2695 117
Size: 341 2237 1936
Fragments arranged by size:
2237 1936 341
NarI GG'CG_CC
Cuts at: 335 1945 2193 335
Size: 1610 248 2656
Fragments arranged by size:
2656 1610 248
NcoI C'CATG_G
Cuts at: 107 1226 107
Size: 1119 3395
NgoMIV G'CCGG_C
Cuts at: 115 456 2693 115
Size: 341 2237 1936
Fragments arranged by size:
2237 1936 341
NspI r_CATG'y
Cuts at: 195 1115 1302 1347 1567 2067 2974 195
Size: 920 187 45 220 500 907 1735
Fragments arranged by size:
1735 920 907 500 220 187 45
PasI CC'CwG_GG
Cuts at: 1486 1486
Size: 4514
PciI A'CATG_T
Cuts at: 1343 2970 1343
Size: 1627 2887
PflMI CCAn_nnn'nTGG
Cuts at: 1059 1059
Size: 4514
PfoI T'CCnGG_A
Cuts at: 1019 1028 1271 1019
Size: 9 243 4262
Fragments arranged by size:
4262 243 9
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4482
PpuMI rG'GwC_Cy
Cuts at: 198 515 579 824 1035 1146 198
Size: 317 64 245 211 111 3566
Fragments arranged by size:
3566 317 245 211 111 64
PspOMI G'GGCC_C
Cuts at: 701 1049 701
Size: 348 4166
PspXI vC'TCGA_Gb
Cuts at: 1428 1428
Size: 4514
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1162 1194 1162
Size: 32 4482
PstI C_TGCA'G
Cuts at: 465 1561 2246 465
Size: 1096 685 2733
Fragments arranged by size:
2733 1096 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 609 1287 1939 2299 609
Size: 678 652 360 2824
Fragments arranged by size:
2824 678 652 360
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 1028 1528 1028
Size: 500 4014
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SanDI GG'GwC_CC
Cuts at: 515 515
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 2537 2747 2537
Size: 210 4304
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 802 4031 802
Size: 3229 1285
SexAI A'CCwGG_T
Cuts at: 581 581
Size: 4514
SfcI C'TryA_G
Cuts at: 33 414 461 1557 2242 3235 3426 33
Size: 381 47 1096 685 993 191 1121
Fragments arranged by size:
1121 1096 993 685 381 191 47
SfoI GGC'GCC
Cuts at: 336 1946 2194 336
Size: 1610 248 2656
Fragments arranged by size:
2656 1610 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 145 699 1536 145
Size: 554 837 3123
Fragments arranged by size:
3123 837 554
SmlI C'TyrA_G
Cuts at: 850 1428 1669 2584 3076 3338 850
Size: 578 241 915 492 262 2026
Fragments arranged by size:
2026 915 578 492 262 241
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 195 1567 2067 195
Size: 1372 500 2642
Fragments arranged by size:
2642 1372 500
StyI C'CwwG_G
Cuts at: 107 1226 1324 1648 2002 107
Size: 1119 98 324 354 2619
Fragments arranged by size:
2619 1119 354 324 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2097 2230 2689 2898 2097
Size: 133 459 209 3713
Fragments arranged by size:
3713 459 209 133
TatI w'GTAC_w
Cuts at: 800 1346 4029 800
Size: 546 2683 1285
Fragments arranged by size:
2683 1285 546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 234 266 1487 1519 4031 4063 234
Size: 32 1221 32 2512 32 685
Fragments arranged by size:
2512 1221 685 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2009 2009
Size: 4514
XhoI C'TCGA_G
Cuts at: 1428 1428
Size: 4514
XmaI C'CCGG_G
Cuts at: 143 697 1534 143
Size: 554 837 3123
Fragments arranged by size:
3123 837 554
ZraI GAC'GTC
Cuts at: 276 276
Size: 4514
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsiWI BssHII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI