Restriction map for
pF1KB7418
(Circular) (Six-base) MAPSORT of: pF1KB7418.seq Check: 8248 from: 1 to: 4244
pF1KB7418 4244 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 2501 3248 3516 2069
Size: 432 747 268 2797
Fragments arranged by size:
2797 747 432 268
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1213 1245 1213
Size: 32 4212
AlwNI CAG_nnn'CTG
Cuts at: 627 867 1549 3116 627
Size: 240 682 1567 1755
Fragments arranged by size:
1755 1567 682 240
ApaI G_GGCC'C
Cuts at: 115 224 549 115
Size: 109 325 3810
Fragments arranged by size:
3810 325 109
ApaLI G'TGCA_C
Cuts at: 903 3014 903
Size: 2111 2133
ApoI r'AATT_y
Cuts at: 1204 1248 3597 3853 1204
Size: 44 2349 256 1595
Fragments arranged by size:
2349 1595 256 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 223 1264 223
Size: 1041 3203
BamHI G'GATC_C
Cuts at: 1269 1269
Size: 4244
BanI G'GyrC_C
Cuts at: 363 420 559 1260 1674 1922 1957 363
Size: 57 139 701 414 248 35 2650
Fragments arranged by size:
2650 701 414 248 139 57 35
BanII G_rGCy'C
Cuts at: 115 224 279 549 962 1258 2288 115
Size: 109 55 270 413 296 1030 2071
Fragments arranged by size:
2071 1030 413 296 270 109 55
BbeI G_GCGC'C
Cuts at: 367 1678 1926 367
Size: 1311 248 2685
Fragments arranged by size:
2685 1311 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 894 928 894
Size: 34 4210
BciVI GTATCCnnnnn_n'
Cuts at: 2142 2903 4244 2142
Size: 761 1341 2142
Fragments arranged by size:
2142 1341 761
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BfrBI ATG'CAT
Cuts at: 685 685
Size: 4244
BglI GCCn_nnn'nGGC
Cuts at: 204 361 830 204
Size: 157 469 3618
Fragments arranged by size:
3618 469 157
BglII A'GATC_T
Cuts at: 1759 3796 1759
Size: 2037 2207
BlpI GC'TnA_GC
Cuts at: 1036 1356 1036
Size: 320 3924
Bme1580I G_kGCm'C
Cuts at: 115 224 549 843 907 1869 1962 3018 115
Size: 109 325 294 64 962 93 1056 1341
Fragments arranged by size:
1341 1056 962 325 294 109 93 64
BmgBI CAC'GTC
Cuts at: 297 297
Size: 4244
BmrI ACTGGGnnnn_n'
Cuts at: 468 1456 1537 1872 4016 468
Size: 988 81 335 2144 696
Fragments arranged by size:
2144 988 696 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1165 349
Size: 816 3428
Bpu10I CC'TnA_GC
Cuts at: 631 631
Size: 4244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1420 2299 2791 3089 1000
Size: 420 879 492 298 2155
Fragments arranged by size:
2155 879 492 420 298
BsaI GGTCTCn'nnnn_
Cuts at: 1069 3948 1069
Size: 2879 1365
BsaAI yAC'GTr
Cuts at: 453 1597 453
Size: 1144 3100
BsaBI GATnn'nnATC
Cuts at: 6 397 1758 3795 6
Size: 391 1361 2037 455
Fragments arranged by size:
2037 1361 455 391
BsaHI Gr'CG_yC
Cuts at: 335 364 937 1675 1923 2625 4121 335
Size: 29 573 738 248 702 1496 458
Fragments arranged by size:
1496 738 702 573 458 248 29
BsaWI w'CCGG_w
Cuts at: 1433 1954 2632 2906 3053 3464 1433
Size: 521 678 274 147 411 2213
Fragments arranged by size:
2213 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 956 986 1103 1133 1213 1243 956
Size: 30 117 30 80 30 3957
Fragments arranged by size:
3957 117 80 30 30 30
BseYI C'CCAG_C
Cuts at: 207 372 620 926 1669 2427 3004 3500 207
Size: 165 248 306 743 758 577 496 951
Fragments arranged by size:
951 758 743 577 496 306 248 165
BsiEI CG_ry'CG
Cuts at: 104 257 381 1786 1832 3040 104
Size: 153 124 1405 46 1208 1308
Fragments arranged by size:
1405 1308 1208 153 124 46
BsiHKAI G_wGCw'C
Cuts at: 907 962 1258 2036 2226 3018 907
Size: 55 296 778 190 792 2133
Fragments arranged by size:
2133 792 778 296 190 55
BsmBI CGTCTCn'nnnn_
Cuts at: 173 173
Size: 4244
Bsp1286I G_dGCh'C
Cuts at: 115 224 279 549 843 907 962 1258 1869
Size: 109 55 270 294 64 55 296 611
Cuts at: 1869 1962 2036 2226 2288 3018 115
Size: 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 296 294 270 190 109
93 74 64 62 55 55
BspEI T'CCGG_A
Cuts at: 3464 3464
Size: 4244
BspHI T'CATG_A
Cuts at: 3420 3420
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1198 1294 1508 1810 2191 1198
Size: 96 214 302 381 3251
Fragments arranged by size:
3251 381 302 214 96
BsrBI CCG'CTC
Cuts at: 50 121 2536 2590 4074 4239 50
Size: 71 2415 54 1484 165 55
Fragments arranged by size:
2415 1484 165 71 55 54
BsrDI GCAATG_nn'
Cuts at: 685 2156 685
Size: 1471 2773
BsrFI r'CCGG_y
Cuts at: 249 1031 2242 2423 2632 249
Size: 782 1211 181 209 1861
Fragments arranged by size:
1861 1211 782 209 181
BssSI C'ACGA_G
Cuts at: 913 1141 2515 2873 913
Size: 228 1374 358 2284
Fragments arranged by size:
2284 1374 358 228
BstAPI GCAn_nnn'nTGC
Cuts at: 1621 1621
Size: 4244
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 525 678 885 525
Size: 153 207 3884
Fragments arranged by size:
3884 207 153
BstYI r'GATC_y
Cuts at: 1 1062 1269 1736 1759 2094 2340 3352 3438
Size: 1061 207 467 23 335 246 1012 86
Cuts at: 3438 3450 3796 1
Size: 12 346 449
Fragments arranged by size:
1061 1012 467 449 346 335 246 207
86 23 12
BtgI C'CryG_G
Cuts at: 107 230 707 107
Size: 123 477 3644
Fragments arranged by size:
3644 477 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2194 2375 2538 2194
Size: 181 163 3900
Fragments arranged by size:
3900 181 163
BtsI GCAGTG_nn'
Cuts at: 449 504 1487 449
Size: 55 983 3206
Fragments arranged by size:
3206 983 55
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DraIII CAC_nnn'GTG
Cuts at: 597 597
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 322 1950 2808 322
Size: 1628 858 1758
Fragments arranged by size:
1758 1628 858
EaeI y'GGCC_r
Cuts at: 254 890 1829 2003 2394 2421 3766 254
Size: 636 939 174 391 27 1345 732
Fragments arranged by size:
1345 939 732 636 391 174 27
EagI C'GGCC_G
Cuts at: 254 1829 254
Size: 1575 2669
EarI CTCTTCn'nnn_
Cuts at: 556 2267 2477 556
Size: 1711 210 2323
Fragments arranged by size:
2323 1711 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 143 250 287 2452 2762 2908 4054 143
Size: 107 37 2165 310 146 1146 333
Fragments arranged by size:
2165 1146 333 310 146 107 37
EcoICRI GAG'CTC
Cuts at: 960 1256 960
Size: 296 3948
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1165 2069 2501 3248 3516 349
Size: 816 904 432 747 268 1077
Fragments arranged by size:
1077 904 816 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 787 787
Size: 4244
EcoO109I rG'GnC_Cy
Cuts at: 112 220 522 546 698 1053 1383 112
Size: 108 302 24 152 355 330 2973
Fragments arranged by size:
2973 355 330 302 152 108 24
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 780 2263 780
Size: 1483 2761
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 367 990 1029 1678 1926 2948 3927 367
Size: 623 39 649 248 1022 979 684
Fragments arranged by size:
1022 979 684 649 623 248 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 721 753 2086 2116 2118 2148 721
Size: 32 1333 30 2 30 2817
Fragments arranged by size:
2817 1333 32 30 30 2
HincII GTy'rAC
Cuts at: 761 1283 761
Size: 522 3722
KasI G'GCGC_C
Cuts at: 363 1674 1922 363
Size: 1311 248 2685
Fragments arranged by size:
2685 1311 248
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 659 2915 3099 659
Size: 2256 184 1804
Fragments arranged by size:
2256 1804 184
MscI TGG'CCA
Cuts at: 892 2005 892
Size: 1113 3131
MslI CAynn'nnrTG
Cuts at: 2233 2360 2233
Size: 127 4117
MspA1I CmG'CkG
Cuts at: 174 201 232 725 867 930 954 1355 1669
Size: 27 31 493 142 63 24 401 314
Cuts at: 1669 2029 3042 3287 174
Size: 360 1013 245 1131
Fragments arranged by size:
1131 1013 493 401 360 314 245 142
63 31 27 24
NaeI GCC'GGC
Cuts at: 251 1033 2425 251
Size: 782 1392 2070
Fragments arranged by size:
2070 1392 782
NarI GG'CG_CC
Cuts at: 364 1675 1923 364
Size: 1311 248 2685
Fragments arranged by size:
2685 1311 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4244
NgoMIV G'CCGG_C
Cuts at: 249 1031 2423 249
Size: 782 1392 2070
Fragments arranged by size:
2070 1392 782
NsiI A_TGCA'T
Cuts at: 687 687
Size: 4244
NspI r_CATG'y
Cuts at: 1297 1797 2704 1297
Size: 500 907 2837
Fragments arranged by size:
2837 907 500
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PflMI CCAn_nnn'nTGG
Cuts at: 597 597
Size: 4244
PfoI T'CCnGG_A
Cuts at: 326 326
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1213 1245 3383 3415 1213
Size: 32 2138 32 2042
Fragments arranged by size:
2138 2042 32 32
PpuMI rG'GwC_Cy
Cuts at: 698 698
Size: 4244
PspOMI G'GGCC_C
Cuts at: 111 220 545 111
Size: 109 325 3810
Fragments arranged by size:
3810 325 109
PstI C_TGCA'G
Cuts at: 695 863 1291 1976 695
Size: 168 428 685 2963
Fragments arranged by size:
2963 685 428 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 867 1669 2029 867
Size: 802 360 3082
Fragments arranged by size:
3082 802 360
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 962 1258 962
Size: 296 3948
SacII CC_GC'GG
Cuts at: 233 233
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 691 859 1287 1972 2965 3156 33
Size: 658 168 428 685 993 191 1121
Fragments arranged by size:
1121 993 685 658 428 191 168
SfoI GGC'GCC
Cuts at: 365 1676 1924 365
Size: 1311 248 2685
Fragments arranged by size:
2685 1311 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 225 1266 225
Size: 1041 3203
SmlI C'TyrA_G
Cuts at: 1015 1399 2314 2806 3068 1015
Size: 384 915 492 262 2191
Fragments arranged by size:
2191 915 492 384 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
StuI AGG'CCT
Cuts at: 511 911 511
Size: 400 3844
StyI C'CwwG_G
Cuts at: 107 518 788 825 1378 1732 107
Size: 411 270 37 553 354 2619
Fragments arranged by size:
2619 553 411 354 270 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 547 1827 1960 2419 2628 547
Size: 1280 133 459 209 2163
Fragments arranged by size:
2163 1280 459 209 133
TatI w'GTAC_w
Cuts at: 3759 3759
Size: 4244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 762 794 956 988 3761 3793 762
Size: 32 162 32 2773 32 1213
Fragments arranged by size:
2773 1213 162 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 817 2041 817
Size: 1224 3020
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 431 885 1739 431
Size: 454 854 2936
Fragments arranged by size:
2936 854 454
XmaI C'CCGG_G
Cuts at: 223 1264 223
Size: 1041 3203
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BmtI BseRI BsgI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI