Restriction map for
pF1KB7419
(Circular) (Six-base) MAPSORT of: pF1KB7419.seq Check: 5020 from: 1 to: 4067
pF1KB7419 4067 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1105 1105
Size: 4067
Acc65I G'GTAC_C
Cuts at: 865 1083 865
Size: 218 3849
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1892 2324 3071 3339 1892
Size: 432 747 268 2620
Fragments arranged by size:
2620 747 432 268
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AlwNI CAG_nnn'CTG
Cuts at: 1372 2939 1372
Size: 1567 2500
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 139 1071 3420 3676 139
Size: 932 2349 256 530
Fragments arranged by size:
2349 932 530 256
AseI AT'TA_AT
Cuts at: 20 629 20
Size: 609 3458
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 530 563 530
Size: 33 4034
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4067
BanI G'GyrC_C
Cuts at: 865 1083 1497 1745 1780 865
Size: 218 414 248 35 3152
Fragments arranged by size:
3152 414 248 218 35
BanII G_rGCy'C
Cuts at: 1081 2111 1081
Size: 1030 3037
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BbsI GAAGACnn'nnnn_
Cuts at: 262 813 262
Size: 551 3516
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 242 851 1587 242
Size: 609 736 2722
Fragments arranged by size:
2722 736 609
BglII A'GATC_T
Cuts at: 647 1582 3619 647
Size: 935 2037 1095
Fragments arranged by size:
2037 1095 935
BlpI GC'TnA_GC
Cuts at: 831 1039 1179 831
Size: 208 140 3719
Fragments arranged by size:
3719 208 140
Bme1580I G_kGCm'C
Cuts at: 1692 1785 2841 1692
Size: 93 1056 2918
Fragments arranged by size:
2918 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1279 1360 1695 3839 1279
Size: 81 335 2144 1507
Fragments arranged by size:
2144 1507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1020 1243 2122 2614 2912 196
Size: 824 223 879 492 298 1351
Fragments arranged by size:
1351 879 824 492 298 223
BsaI GGTCTCn'nnnn_
Cuts at: 3771 3771
Size: 4067
BsaAI yAC'GTr
Cuts at: 213 1420 213
Size: 1207 2860
BsaBI GATnn'nnATC
Cuts at: 6 1581 3618 6
Size: 1575 2037 455
Fragments arranged by size:
2037 1575 455
BsaHI Gr'CG_yC
Cuts at: 1498 1746 2448 3944 1498
Size: 248 702 1496 1621
Fragments arranged by size:
1621 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2455 2729 2876 3287 1256
Size: 521 678 274 147 411 2036
Fragments arranged by size:
2036 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 324 324
Size: 4067
BseYI C'CCAG_C
Cuts at: 1492 2250 2827 3323 1492
Size: 758 577 496 2236
Fragments arranged by size:
2236 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 424 419
Size: 5 4062
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1081 1859 2049 2841 1081
Size: 778 190 792 2307
Fragments arranged by size:
2307 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1081 1692 1785 1859 2049 2111 2841 1081
Size: 611 93 74 190 62 730 2307
Fragments arranged by size:
2307 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 512 3243 512
Size: 2731 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1117 1331 1633 2014 1117
Size: 214 302 381 3170
Fragments arranged by size:
3170 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 237 431 1979 237
Size: 194 1548 2325
Fragments arranged by size:
2325 1548 194
BsrFI r'CCGG_y
Cuts at: 2065 2246 2455 2065
Size: 181 209 3677
Fragments arranged by size:
3677 209 181
BssSI C'ACGA_G
Cuts at: 742 2338 2696 742
Size: 1596 358 2113
Fragments arranged by size:
2113 1596 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstEII G'GTnAC_C
Cuts at: 163 163
Size: 4067
BstYI r'GATC_y
Cuts at: 1 647 1092 1559 1582 1917 2163 3175 3261
Size: 646 445 467 23 335 246 1012 86
Cuts at: 3261 3273 3619 1
Size: 12 346 449
Fragments arranged by size:
1012 646 467 449 445 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 615 2017 2198 2361 615
Size: 1402 181 163 2321
Fragments arranged by size:
2321 1402 181 163
BtsI GCAGTG_nn'
Cuts at: 407 713 1310 407
Size: 306 597 3164
Fragments arranged by size:
3164 597 306
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 479 797 1652 1826 2217 2244 3589 479
Size: 318 855 174 391 27 1345 957
Fragments arranged by size:
1345 957 855 391 318 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2585 2731 3877 2275
Size: 310 146 1146 2465
Fragments arranged by size:
2465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1892 2324 3071 3339 1892
Size: 432 747 268 2620
Fragments arranged by size:
2620 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 903 1206 903
Size: 303 3764
EcoRI G'AATT_C
Cuts at: 139 1071 139
Size: 932 3135
EcoRV GAT'ATC
Cuts at: 318 318
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 1501 1749 2771 3750 1501
Size: 248 1022 979 1818
Fragments arranged by size:
1818 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 1909 1939 1941 1971 491
Size: 32 1386 30 2 30 2587
Fragments arranged by size:
2587 1386 32 30 30 2
HincII GTy'rAC
Cuts at: 434 1106 434
Size: 672 3395
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 869 1087 869
Size: 218 3849
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2738 2922 2738
Size: 184 3883
MscI TGG'CCA
Cuts at: 481 1828 481
Size: 1347 2720
MslI CAynn'nnrTG
Cuts at: 280 511 2056 2183 280
Size: 231 1545 127 2164
Fragments arranged by size:
2164 1545 231 127
MspA1I CmG'CkG
Cuts at: 1178 1492 1852 2865 3110 1178
Size: 314 360 1013 245 2135
Fragments arranged by size:
2135 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NcoI C'CATG_G
Cuts at: 107 107
Size: 4067
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NspI r_CATG'y
Cuts at: 279 830 1120 1620 2527 279
Size: 551 290 500 907 1819
Fragments arranged by size:
1819 907 551 500 290
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PflMI CCAn_nnn'nTGG
Cuts at: 602 602
Size: 4067
PfoI T'CCnGG_A
Cuts at: 311 311
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4035
PpuMI rG'GwC_Cy
Cuts at: 903 903
Size: 4067
PstI C_TGCA'G
Cuts at: 1114 1799 1114
Size: 685 3382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 1492 1852 1492
Size: 360 3707
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SexAI A'CCwGG_T
Cuts at: 1058 1058
Size: 4067
SfcI C'TryA_G
Cuts at: 33 932 993 1110 1795 2788 2979 33
Size: 899 61 117 685 993 191 1121
Fragments arranged by size:
1121 993 899 685 191 117 61
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 175 999 1222 2137 2629 2891 175
Size: 824 223 915 492 262 1351
Fragments arranged by size:
1351 915 824 492 262 223
SnaBI TAC'GTA
Cuts at: 213 213
Size: 4067
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
SspI AAT'ATT
Cuts at: 624 693 717 1033 624
Size: 69 24 316 3658
Fragments arranged by size:
3658 316 69 24
StyI C'CwwG_G
Cuts at: 107 450 909 1201 1555 107
Size: 343 459 292 354 2619
Fragments arranged by size:
2619 459 354 343 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1650 1783 2242 2451 1650
Size: 133 459 209 3266
Fragments arranged by size:
3266 459 209 133
TatI w'GTAC_w
Cuts at: 284 531 3582 284
Size: 247 3051 769
Fragments arranged by size:
3051 769 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 386 418 3584 3616 386
Size: 32 3166 32 837
Fragments arranged by size:
3166 837 32 32
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 907 1562 907
Size: 655 3412
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
XmnI GAAnn'nnTTC
Cuts at: 143 381 570 143
Size: 238 189 3640
Fragments arranged by size:
3640 238 189
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SrfI StuI
SwaI XhoI ZraI