Restriction map for
pF1KB7420
(Circular) (Six-base) MAPSORT of: pF1KB7420.seq Check: 3094 from: 1 to: 3920
pF1KB7420 3920 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 494 494
Size: 3920
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1745 2177 2924 3192 1745
Size: 432 747 268 2473
Fragments arranged by size:
2473 747 432 268
AfeI AGC'GCT
Cuts at: 276 276
Size: 3920
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AlwNI CAG_nnn'CTG
Cuts at: 907 1225 2792 907
Size: 318 1567 2035
Fragments arranged by size:
2035 1567 318
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 924 3273 3529 924
Size: 2349 256 1315
Fragments arranged by size:
2349 1315 256
AscI GG'CGCG_CC
Cuts at: 258 258
Size: 3920
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 940 940
Size: 3920
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 477 644 737 936 1350 1598 1633 477
Size: 167 93 199 414 248 35 2764
Fragments arranged by size:
2764 414 248 199 167 93 35
BanII G_rGCy'C
Cuts at: 722 934 1964 722
Size: 212 1030 2678
Fragments arranged by size:
2678 1030 212
BbeI G_GCGC'C
Cuts at: 741 1354 1602 741
Size: 613 248 3059
Fragments arranged by size:
3059 613 248
BbsI GAAGACnn'nnnn_
Cuts at: 525 525
Size: 3920
BbvCI CC'TCA_GC
Cuts at: 619 619
Size: 3920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3886
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BglII A'GATC_T
Cuts at: 144 1435 3472 144
Size: 1291 2037 592
Fragments arranged by size:
2037 1291 592
BlpI GC'TnA_GC
Cuts at: 1032 1032
Size: 3920
Bme1580I G_kGCm'C
Cuts at: 482 647 1545 1638 2694 482
Size: 165 898 93 1056 1708
Fragments arranged by size:
1708 1056 898 165 93
BmrI ACTGGGnnnn_n'
Cuts at: 117 645 1132 1213 1548 3692 117
Size: 528 487 81 335 2144 345
Fragments arranged by size:
2144 528 487 345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 487 499 736 263
Size: 224 12 237 3447
Fragments arranged by size:
3447 237 224 12
Bpu10I CC'TnA_GC
Cuts at: 619 619
Size: 3920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 793 1096 1975 2467 2765 727
Size: 66 303 879 492 298 1882
Fragments arranged by size:
1882 879 492 303 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 874 1434 3471 6
Size: 868 560 2037 455
Fragments arranged by size:
2037 868 560 455
BsaHI Gr'CG_yC
Cuts at: 415 738 1351 1599 2301 3797 415
Size: 323 613 248 702 1496 538
Fragments arranged by size:
1496 702 613 538 323 248
BsaWI w'CCGG_w
Cuts at: 158 1109 1630 2308 2582 2729 3140 158
Size: 951 521 678 274 147 411 938
Fragments arranged by size:
951 938 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 295 325 295
Size: 30 3890
BseYI C'CCAG_C
Cuts at: 1345 2103 2680 3176 1345
Size: 758 577 496 2089
Fragments arranged by size:
2089 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 382
Size: 3920
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 722 934 1712 1902 2694 722
Size: 212 778 190 792 1948
Fragments arranged by size:
1948 792 778 212 190
Bsp1286I G_dGCh'C
Cuts at: 482 647 722 934 1545 1638 1712 1902 1964
Size: 165 75 212 611 93 74 190 62
Cuts at: 1964 2694 482
Size: 730 1708
Fragments arranged by size:
1708 730 611 212 190 165 93 75
74 62
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 494 970 1184 1486 1867 494
Size: 476 214 302 381 2547
Fragments arranged by size:
2547 476 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 1832
Size: 3920
BsrFI r'CCGG_y
Cuts at: 564 1918 2099 2308 564
Size: 1354 181 209 2176
Fragments arranged by size:
2176 1354 209 181
BssHII G'CGCG_C
Cuts at: 258 258
Size: 3920
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 1297 1297
Size: 3920
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstXI CCAn_nnnn'nTGG
Cuts at: 130 130
Size: 3920
BstYI r'GATC_y
Cuts at: 1 144 494 945 1412 1435 1770 2016 3028
Size: 143 350 451 467 23 335 246 1012
Cuts at: 3028 3114 3126 3472 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 451 449 350 346 335 246
143 86 23 12
BtgI C'CryG_G
Cuts at: 542 542
Size: 3920
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 321 1163 321
Size: 842 3078
DraI TTT'AAA
Cuts at: 919 919
Size: 3920
DrdI GACnn_nn'nnGTC
Cuts at: 811 1626 2484 811
Size: 815 858 2247
Fragments arranged by size:
2247 858 815
EaeI y'GGCC_r
Cuts at: 539 597 1505 1679 2070 2097 3442 539
Size: 58 908 174 391 27 1345 1017
Fragments arranged by size:
1345 1017 908 391 174 58 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 844 1943 2153 844
Size: 1099 210 2611
Fragments arranged by size:
2611 1099 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 340 2128 2438 2584 3730 340
Size: 1788 310 146 1146 530
Fragments arranged by size:
1788 1146 530 310 146
EcoICRI GAG'CTC
Cuts at: 720 932 720
Size: 212 3708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 487 499 736 1745 2177 2924 3192 263
Size: 224 12 237 1009 432 747 268 991
Fragments arranged by size:
1009 991 747 432 268 237 224 12
EcoO109I rG'GnC_Cy
Cuts at: 680 891 1059 680
Size: 211 168 3541
Fragments arranged by size:
3541 211 168
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3920
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 278 741 1354 1602 2624 3603 278
Size: 463 613 248 1022 979 595
Fragments arranged by size:
1022 979 613 595 463 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 512 544 1762 1792 1794 1824 512
Size: 32 1218 30 2 30 2608
Fragments arranged by size:
2608 1218 32 30 30 2
HincII GTy'rAC
Cuts at: 222 507 959 222
Size: 285 452 3183
Fragments arranged by size:
3183 452 285
KasI G'GCGC_C
Cuts at: 737 1350 1598 737
Size: 613 248 3059
Fragments arranged by size:
3059 613 248
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2591 2775 2591
Size: 184 3736
MscI TGG'CCA
Cuts at: 541 599 1681 541
Size: 58 1082 2780
Fragments arranged by size:
2780 1082 58
MslI CAynn'nnrTG
Cuts at: 547 667 1909 2036 547
Size: 120 1242 127 2431
Fragments arranged by size:
2431 1242 127 120
MspA1I CmG'CkG
Cuts at: 196 1031 1345 1705 2718 2963 196
Size: 835 314 360 1013 245 1153
Fragments arranged by size:
1153 1013 835 360 314 245
NaeI GCC'GGC
Cuts at: 566 2101 566
Size: 1535 2385
NarI GG'CG_CC
Cuts at: 738 1351 1599 738
Size: 613 248 3059
Fragments arranged by size:
3059 613 248
NgoMIV G'CCGG_C
Cuts at: 564 2099 564
Size: 1535 2385
NspI r_CATG'y
Cuts at: 432 552 973 1473 2380 432
Size: 120 421 500 907 1972
Fragments arranged by size:
1972 907 500 421 120
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PpuMI rG'GwC_Cy
Cuts at: 680 891 680
Size: 211 3709
PshAI GACnn'nnGTC
Cuts at: 889 889
Size: 3920
PstI C_TGCA'G
Cuts at: 967 1652 967
Size: 685 3235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 1345 1705 1345
Size: 360 3560
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 722 934 722
Size: 212 3708
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SanDI GG'GwC_CC
Cuts at: 680 680
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 844 1943 2153 844
Size: 1099 210 2611
Fragments arranged by size:
2611 1099 210
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 963 1648 2641 2832 33
Size: 930 685 993 191 1121
Fragments arranged by size:
1121 993 930 685 191
SfoI GGC'GCC
Cuts at: 739 1352 1600 739
Size: 613 248 3059
Fragments arranged by size:
3059 613 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 742 772 1075 1990 2482 2744 742
Size: 30 303 915 492 262 1918
Fragments arranged by size:
1918 915 492 303 262 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 594 1054 1408 594
Size: 460 354 3106
Fragments arranged by size:
3106 460 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 703 1503 1636 2095 2304 703
Size: 800 133 459 209 2319
Fragments arranged by size:
2319 800 459 209 133
TatI w'GTAC_w
Cuts at: 3435 3435
Size: 3920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 295 327 3437 3469 295
Size: 32 3110 32 746
Fragments arranged by size:
3110 746 32 32
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1415 1415
Size: 3920
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AvrII
BaeI BfrBI BglI BmgBI BmtI BseRI BsiWI BsmI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI