(Circular) (Six-base) MAPSORT of: pF1KB7423.seq Check: 9578 from: 1 to: 3842 pF1KB7423 3842 bp With 182 enzymes: SgfI * June 20, 2008 10:27 .. AccI GT'mk_AC Cuts at: 880 880 Size: 3842 Acc65I G'GTAC_C Cuts at: 858 858 Size: 3842 AclI AA'CG_TT Cuts at: 3682 3682 Size: 3842 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 297 1667 2099 2846 3114 297 Size: 1370 432 747 268 1025 Fragments arranged by size: 1370 1025 747 432 268 AflIII A'CryG_T Cuts at: 139 1194 2298 3275 139 Size: 1055 1104 977 706 Fragments arranged by size: 1104 1055 977 706 AgeI A'CCGG_T Cuts at: 2230 2230 Size: 3842 AleI CACnn'nnGTG Cuts at: 544 544 Size: 3842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 562 562 Size: 3842 AlwNI CAG_nnn'CTG Cuts at: 186 1147 2714 186 Size: 961 1567 1314 Fragments arranged by size: 1567 1314 961 ApaLI G'TGCA_C Cuts at: 547 687 2612 547 Size: 140 1925 1777 Fragments arranged by size: 1925 1777 140 ApoI r'AATT_y Cuts at: 846 3195 3451 846 Size: 2349 256 1237 Fragments arranged by size: 2349 1237 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3842 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3842 AvaI C'yCGr_G Cuts at: 862 862 Size: 3842 BamHI G'GATC_C Cuts at: 867 867 Size: 3842 BanI G'GyrC_C Cuts at: 306 858 1272 1520 1555 306 Size: 552 414 248 35 2593 Fragments arranged by size: 2593 552 414 248 35 BanII G_rGCy'C Cuts at: 856 1886 856 Size: 1030 2812 BbeI G_GCGC'C Cuts at: 1276 1524 1276 Size: 248 3594 BbsI GAAGACnn'nnnn_ Cuts at: 684 684 Size: 3842 BciVI GTATCCnnnnn_n' Cuts at: 1740 2501 3842 1740 Size: 761 1341 1740 Fragments arranged by size: 1740 1341 761 BclI T'GATC_A Cuts at: 291 1362 291 Size: 1071 2771 BglII A'GATC_T Cuts at: 1357 3394 1357 Size: 2037 1805 BlpI GC'TnA_GC Cuts at: 954 954 Size: 3842 Bme1580I G_kGCm'C Cuts at: 311 487 551 691 1467 1560 2616 311 Size: 176 64 140 776 93 1056 1537 Fragments arranged by size: 1537 1056 776 176 140 93 64 BmrI ACTGGGnnnn_n' Cuts at: 838 1054 1135 1470 3614 838 Size: 216 81 335 2144 1066 Fragments arranged by size: 2144 1066 335 216 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2209 2209 Size: 3842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 194 640 734 194 Size: 446 94 3302 Fragments arranged by size: 3302 446 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1018 1897 2389 2687 1018 Size: 879 492 298 2173 Fragments arranged by size: 2173 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3546 3546 Size: 3842 BsaAI yAC'GTr Cuts at: 552 1195 552 Size: 643 3199 BsaBI GATnn'nnATC Cuts at: 6 1356 3393 6 Size: 1350 2037 455 Fragments arranged by size: 2037 1350 455 BsaHI Gr'CG_yC Cuts at: 1273 1521 2223 3719 1273 Size: 248 702 1496 1396 Fragments arranged by size: 1496 1396 702 248 BsaWI w'CCGG_w Cuts at: 1031 1552 2230 2504 2651 3062 1031 Size: 521 678 274 147 411 1811 Fragments arranged by size: 1811 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 536 566 536 Size: 30 3812 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 264 269 586 612 637 664 678 264 Size: 5 317 26 25 27 14 3428 Fragments arranged by size: 3428 317 27 26 25 14 5 BseYI C'CCAG_C Cuts at: 703 1267 2025 2602 3098 703 Size: 564 758 577 496 1447 Fragments arranged by size: 1447 758 577 564 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 675 675 Size: 3842 BsiEI CG_ry'CG Cuts at: 104 1384 1430 2638 104 Size: 1280 46 1208 1308 Fragments arranged by size: 1308 1280 1208 46 BsiHKAI G_wGCw'C Cuts at: 551 670 691 856 1634 1824 2616 551 Size: 119 21 165 778 190 792 1777 Fragments arranged by size: 1777 792 778 190 165 119 21 BsmBI CGTCTCn'nnnn_ Cuts at: 170 170 Size: 3842 Bsp1286I G_dGCh'C Cuts at: 311 487 551 670 691 856 1467 1560 1634 Size: 176 64 119 21 165 611 93 74 Cuts at: 1634 1824 1886 2616 311 Size: 190 62 730 1537 Fragments arranged by size: 1537 730 611 190 176 165 119 93 74 64 62 21 BspEI T'CCGG_A Cuts at: 3062 3062 Size: 3842 BspHI T'CATG_A Cuts at: 3018 3018 Size: 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 892 1106 1408 1789 892 Size: 214 302 381 2945 Fragments arranged by size: 2945 381 302 214 BsrBI CCG'CTC Cuts at: 50 2134 2188 3672 3837 50 Size: 2084 54 1484 165 55 Fragments arranged by size: 2084 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 767 1754 767 Size: 987 2855 BsrFI r'CCGG_y Cuts at: 1840 2021 2230 1840 Size: 181 209 3452 Fragments arranged by size: 3452 209 181 BssSI C'ACGA_G Cuts at: 2113 2471 2113 Size: 358 3484 BstAPI GCAn_nnn'nTGC Cuts at: 482 1219 482 Size: 737 3105 BstBI TT'CG_AA Cuts at: 2203 2203 Size: 3842 BstYI r'GATC_y Cuts at: 1 608 867 1334 1357 1692 1938 2950 3036 Size: 607 259 467 23 335 246 1012 86 Cuts at: 3036 3048 3394 1 Size: 12 346 449 Fragments arranged by size: 1012 607 467 449 346 335 259 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3842 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1792 1973 2136 1792 Size: 181 163 3498 Fragments arranged by size: 3498 181 163 BtsI GCAGTG_nn' Cuts at: 799 1085 799 Size: 286 3556 DraI TTT'AAA Cuts at: 841 841 Size: 3842 DrdI GACnn_nn'nnGTC Cuts at: 1548 2406 1548 Size: 858 2984 EaeI y'GGCC_r Cuts at: 1427 1601 1992 2019 3364 1427 Size: 174 391 27 1345 1905 Fragments arranged by size: 1905 1345 391 174 27 EagI C'GGCC_G Cuts at: 1427 1427 Size: 3842 EarI CTCTTCn'nnn_ Cuts at: 780 1865 2075 780 Size: 1085 210 2547 Fragments arranged by size: 2547 1085 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2050 2360 2506 3652 2050 Size: 310 146 1146 2240 Fragments arranged by size: 2240 1146 310 146 EcoICRI GAG'CTC Cuts at: 854 854 Size: 3842 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 194 297 640 734 1667 2099 2846 3114 194 Size: 103 343 94 933 432 747 268 922 Fragments arranged by size: 933 922 747 432 343 268 103 94 EcoO109I rG'GnC_Cy Cuts at: 981 981 Size: 3842 EcoRI G'AATT_C Cuts at: 846 846 Size: 3842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1861 1861 Size: 3842 FspI TGC'GCA Cuts at: 1623 1623 Size: 3842 HaeII r_GCGC'y Cuts at: 124 1276 1524 2546 3525 124 Size: 1152 248 1022 979 441 Fragments arranged by size: 1152 1022 979 441 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1684 1714 1716 1746 1684 Size: 30 2 30 3780 Fragments arranged by size: 3780 30 30 2 HincII GTy'rAC Cuts at: 342 881 342 Size: 539 3303 KasI G'GCGC_C Cuts at: 1272 1520 1272 Size: 248 3594 KpnI G_GTAC'C Cuts at: 862 862 Size: 3842 MluI A'CGCG_T Cuts at: 3275 3275 Size: 3842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2513 2697 2513 Size: 184 3658 MscI TGG'CCA Cuts at: 1603 1603 Size: 3842 MslI CAynn'nnrTG Cuts at: 195 544 1831 1958 195 Size: 349 1287 127 2079 Fragments arranged by size: 2079 1287 349 127 MspA1I CmG'CkG Cuts at: 186 953 1267 1627 2640 2885 186 Size: 767 314 360 1013 245 1143 Fragments arranged by size: 1143 1013 767 360 314 245 NaeI GCC'GGC Cuts at: 2023 2023 Size: 3842 NarI GG'CG_CC Cuts at: 1273 1521 1273 Size: 248 3594 NcoI C'CATG_G Cuts at: 107 107 Size: 3842 NgoMIV G'CCGG_C Cuts at: 2021 2021 Size: 3842 NspI r_CATG'y Cuts at: 143 503 895 1395 2302 143 Size: 360 392 500 907 1683 Fragments arranged by size: 1683 907 500 392 360 PasI CC'CwG_GG Cuts at: 784 784 Size: 3842 PciI A'CATG_T Cuts at: 139 2298 139 Size: 2159 1683 PfoI T'CCnGG_A Cuts at: 520 520 Size: 3842 PmeI GTTT'AAAC Cuts at: 841 841 Size: 3842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2981 3013 2981 Size: 32 3810 PstI C_TGCA'G Cuts at: 889 1574 889 Size: 685 3157 PvuI CG_AT'CG Cuts at: 104 104 Size: 3842 PvuII CAG'CTG Cuts at: 1267 1627 1267 Size: 360 3482 RsrII CG'GwC_CG Cuts at: 2037 2037 Size: 3842 SacI G_AGCT'C Cuts at: 856 856 Size: 3842 SalI G'TCGA_C Cuts at: 879 879 Size: 3842 SapI GCTCTTCn'nnn_ Cuts at: 1865 2075 1865 Size: 210 3632 SbfI CC_TGCA'GG Cuts at: 889 889 Size: 3842 ScaI AGT'ACT Cuts at: 3359 3359 Size: 3842 SfcI C'TryA_G Cuts at: 33 399 885 1570 2563 2754 33 Size: 366 486 685 993 191 1121 Fragments arranged by size: 1121 993 685 486 366 191 SfoI GGC'GCC Cuts at: 1274 1522 1274 Size: 248 3594 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3842 SmaI CCC'GGG Cuts at: 864 864 Size: 3842 SmlI C'TyrA_G Cuts at: 997 1912 2404 2666 997 Size: 915 492 262 2173 Fragments arranged by size: 2173 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3842 SphI G_CATG'C Cuts at: 895 1395 895 Size: 500 3342 StyI C'CwwG_G Cuts at: 107 285 560 976 1330 107 Size: 178 275 416 354 2619 Fragments arranged by size: 2619 416 354 275 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 717 1425 1558 2017 2226 717 Size: 708 133 459 209 2333 Fragments arranged by size: 2333 708 459 209 133 TatI w'GTAC_w Cuts at: 3357 3357 Size: 3842 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3359 3391 3359 Size: 32 3810 Tth111I GACn'n_nGTC Cuts at: 1639 1639 Size: 3842 XbaI T'CTAG_A Cuts at: 873 873 Size: 3842 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 1337 114 Size: 1223 2619 XmaI C'CCGG_G Cuts at: 862 862 Size: 3842 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI