Restriction map for
pF1KB7423
(Circular) (Six-base) MAPSORT of: pF1KB7423.seq Check: 9578 from: 1 to: 3842
pF1KB7423 3842 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 1667 2099 2846 3114 297
Size: 1370 432 747 268 1025
Fragments arranged by size:
1370 1025 747 432 268
AflIII A'CryG_T
Cuts at: 139 1194 2298 3275 139
Size: 1055 1104 977 706
Fragments arranged by size:
1104 1055 977 706
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AleI CACnn'nnGTG
Cuts at: 544 544
Size: 3842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 562 562
Size: 3842
AlwNI CAG_nnn'CTG
Cuts at: 186 1147 2714 186
Size: 961 1567 1314
Fragments arranged by size:
1567 1314 961
ApaLI G'TGCA_C
Cuts at: 547 687 2612 547
Size: 140 1925 1777
Fragments arranged by size:
1925 1777 140
ApoI r'AATT_y
Cuts at: 846 3195 3451 846
Size: 2349 256 1237
Fragments arranged by size:
2349 1237 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 862 862
Size: 3842
BamHI G'GATC_C
Cuts at: 867 867
Size: 3842
BanI G'GyrC_C
Cuts at: 306 858 1272 1520 1555 306
Size: 552 414 248 35 2593
Fragments arranged by size:
2593 552 414 248 35
BanII G_rGCy'C
Cuts at: 856 1886 856
Size: 1030 2812
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BbsI GAAGACnn'nnnn_
Cuts at: 684 684
Size: 3842
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2501 3842 1740
Size: 761 1341 1740
Fragments arranged by size:
1740 1341 761
BclI T'GATC_A
Cuts at: 291 1362 291
Size: 1071 2771
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 954 954
Size: 3842
Bme1580I G_kGCm'C
Cuts at: 311 487 551 691 1467 1560 2616 311
Size: 176 64 140 776 93 1056 1537
Fragments arranged by size:
1537 1056 776 176 140 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 838 1054 1135 1470 3614 838
Size: 216 81 335 2144 1066
Fragments arranged by size:
2144 1066 335 216 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 3842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 640 734 194
Size: 446 94 3302
Fragments arranged by size:
3302 446 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1897 2389 2687 1018
Size: 879 492 298 2173
Fragments arranged by size:
2173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3546 3546
Size: 3842
BsaAI yAC'GTr
Cuts at: 552 1195 552
Size: 643 3199
BsaBI GATnn'nnATC
Cuts at: 6 1356 3393 6
Size: 1350 2037 455
Fragments arranged by size:
2037 1350 455
BsaHI Gr'CG_yC
Cuts at: 1273 1521 2223 3719 1273
Size: 248 702 1496 1396
Fragments arranged by size:
1496 1396 702 248
BsaWI w'CCGG_w
Cuts at: 1031 1552 2230 2504 2651 3062 1031
Size: 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 536 566 536
Size: 30 3812
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 264 269 586 612 637 664 678 264
Size: 5 317 26 25 27 14 3428
Fragments arranged by size:
3428 317 27 26 25 14 5
BseYI C'CCAG_C
Cuts at: 703 1267 2025 2602 3098 703
Size: 564 758 577 496 1447
Fragments arranged by size:
1447 758 577 564 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 675 675
Size: 3842
BsiEI CG_ry'CG
Cuts at: 104 1384 1430 2638 104
Size: 1280 46 1208 1308
Fragments arranged by size:
1308 1280 1208 46
BsiHKAI G_wGCw'C
Cuts at: 551 670 691 856 1634 1824 2616 551
Size: 119 21 165 778 190 792 1777
Fragments arranged by size:
1777 792 778 190 165 119 21
BsmBI CGTCTCn'nnnn_
Cuts at: 170 170
Size: 3842
Bsp1286I G_dGCh'C
Cuts at: 311 487 551 670 691 856 1467 1560 1634
Size: 176 64 119 21 165 611 93 74
Cuts at: 1634 1824 1886 2616 311
Size: 190 62 730 1537
Fragments arranged by size:
1537 730 611 190 176 165 119 93
74 64 62 21
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 3018 3018
Size: 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1106 1408 1789 892
Size: 214 302 381 2945
Fragments arranged by size:
2945 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2134 2188 3672 3837 50
Size: 2084 54 1484 165 55
Fragments arranged by size:
2084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 767 1754 767
Size: 987 2855
BsrFI r'CCGG_y
Cuts at: 1840 2021 2230 1840
Size: 181 209 3452
Fragments arranged by size:
3452 209 181
BssSI C'ACGA_G
Cuts at: 2113 2471 2113
Size: 358 3484
BstAPI GCAn_nnn'nTGC
Cuts at: 482 1219 482
Size: 737 3105
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstYI r'GATC_y
Cuts at: 1 608 867 1334 1357 1692 1938 2950 3036
Size: 607 259 467 23 335 246 1012 86
Cuts at: 3036 3048 3394 1
Size: 12 346 449
Fragments arranged by size:
1012 607 467 449 346 335 259 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3842
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1792 1973 2136 1792
Size: 181 163 3498
Fragments arranged by size:
3498 181 163
BtsI GCAGTG_nn'
Cuts at: 799 1085 799
Size: 286 3556
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2406 1548
Size: 858 2984
EaeI y'GGCC_r
Cuts at: 1427 1601 1992 2019 3364 1427
Size: 174 391 27 1345 1905
Fragments arranged by size:
1905 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1427 1427
Size: 3842
EarI CTCTTCn'nnn_
Cuts at: 780 1865 2075 780
Size: 1085 210 2547
Fragments arranged by size:
2547 1085 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2050 2360 2506 3652 2050
Size: 310 146 1146 2240
Fragments arranged by size:
2240 1146 310 146
EcoICRI GAG'CTC
Cuts at: 854 854
Size: 3842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 297 640 734 1667 2099 2846 3114 194
Size: 103 343 94 933 432 747 268 922
Fragments arranged by size:
933 922 747 432 343 268 103 94
EcoO109I rG'GnC_Cy
Cuts at: 981 981
Size: 3842
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3842
FspI TGC'GCA
Cuts at: 1623 1623
Size: 3842
HaeII r_GCGC'y
Cuts at: 124 1276 1524 2546 3525 124
Size: 1152 248 1022 979 441
Fragments arranged by size:
1152 1022 979 441 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1684 1714 1716 1746 1684
Size: 30 2 30 3780
Fragments arranged by size:
3780 30 30 2
HincII GTy'rAC
Cuts at: 342 881 342
Size: 539 3303
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2697 2513
Size: 184 3658
MscI TGG'CCA
Cuts at: 1603 1603
Size: 3842
MslI CAynn'nnrTG
Cuts at: 195 544 1831 1958 195
Size: 349 1287 127 2079
Fragments arranged by size:
2079 1287 349 127
MspA1I CmG'CkG
Cuts at: 186 953 1267 1627 2640 2885 186
Size: 767 314 360 1013 245 1143
Fragments arranged by size:
1143 1013 767 360 314 245
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 3842
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NcoI C'CATG_G
Cuts at: 107 107
Size: 3842
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 3842
NspI r_CATG'y
Cuts at: 143 503 895 1395 2302 143
Size: 360 392 500 907 1683
Fragments arranged by size:
1683 907 500 392 360
PasI CC'CwG_GG
Cuts at: 784 784
Size: 3842
PciI A'CATG_T
Cuts at: 139 2298 139
Size: 2159 1683
PfoI T'CCnGG_A
Cuts at: 520 520
Size: 3842
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 3810
PstI C_TGCA'G
Cuts at: 889 1574 889
Size: 685 3157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 1267 1627 1267
Size: 360 3482
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 856 856
Size: 3842
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SfcI C'TryA_G
Cuts at: 33 399 885 1570 2563 2754 33
Size: 366 486 685 993 191 1121
Fragments arranged by size:
1121 993 685 486 366 191
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 997 1912 2404 2666 997
Size: 915 492 262 2173
Fragments arranged by size:
2173 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StyI C'CwwG_G
Cuts at: 107 285 560 976 1330 107
Size: 178 275 416 354 2619
Fragments arranged by size:
2619 416 354 275 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 717 1425 1558 2017 2226 717
Size: 708 133 459 209 2333
Fragments arranged by size:
2333 708 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 3810
Tth111I GACn'n_nGTC
Cuts at: 1639 1639
Size: 3842
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1337 114
Size: 1223 2619
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI