Restriction map for
pF1KB7424
(Circular) (Six-base) MAPSORT of: pF1KB7424.seq Check: 7479 from: 1 to: 4559
pF1KB7424 4559 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 1597 1597
Size: 4559
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 4399 4399
Size: 4559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2816 3563 3831 2384
Size: 432 747 268 3112
Fragments arranged by size:
3112 747 432 268
AflIII A'CryG_T
Cuts at: 1911 3015 3992 1911
Size: 1104 977 2478
Fragments arranged by size:
2478 1104 977
AgeI A'CCGG_T
Cuts at: 2947 2947
Size: 4559
AlwNI CAG_nnn'CTG
Cuts at: 1036 1405 1864 3431 1036
Size: 369 459 1567 2164
Fragments arranged by size:
2164 1567 459 369
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 1098 1116 1563 3912 4168 1098
Size: 18 447 2349 256 1489
Fragments arranged by size:
2349 1489 447 256 18
AseI AT'TA_AT
Cuts at: 20 1157 1507 20
Size: 1137 350 3072
Fragments arranged by size:
3072 1137 350
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 1579 1579
Size: 4559
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 1575 1989 2237 2272 1575
Size: 414 248 35 3862
Fragments arranged by size:
3862 414 248 35
BanII G_rGCy'C
Cuts at: 384 1573 2603 384
Size: 1189 1030 2340
Fragments arranged by size:
2340 1189 1030
BbeI G_GCGC'C
Cuts at: 1993 2241 1993
Size: 248 4311
BbsI GAAGACnn'nnnn_
Cuts at: 905 905
Size: 4559
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 596 630 1533 1567 596
Size: 34 903 34 3588
Fragments arranged by size:
3588 903 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 875 2457 3218 4559 875
Size: 1582 761 1341 875
Fragments arranged by size:
1582 1341 875 761
BclI T'GATC_A
Cuts at: 1134 2079 1134
Size: 945 3614
BglII A'GATC_T
Cuts at: 2074 4111 2074
Size: 2037 2522
BlpI GC'TnA_GC
Cuts at: 1671 1671
Size: 4559
Bme1580I G_kGCm'C
Cuts at: 2184 2277 3333 2184
Size: 93 1056 3410
Fragments arranged by size:
3410 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1771 1852 2187 4331 1771
Size: 81 335 2144 1999
Fragments arranged by size:
2144 1999 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 239 241 2926 239
Size: 2 2685 1872
Fragments arranged by size:
2685 1872 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 1061 1089 396
Size: 665 28 3866
Fragments arranged by size:
3866 665 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1290 1735 2614 3106 3404 1290
Size: 445 879 492 298 2445
Fragments arranged by size:
2445 879 492 445 298
BsaI GGTCTCn'nnnn_
Cuts at: 4263 4263
Size: 4559
BsaAI yAC'GTr
Cuts at: 1912 1912
Size: 4559
BsaBI GATnn'nnATC
Cuts at: 6 140 1451 2073 4110 6
Size: 134 1311 622 2037 455
Fragments arranged by size:
2037 1311 622 455 134
BsaHI Gr'CG_yC
Cuts at: 1990 2238 2940 4436 1990
Size: 248 702 1496 2113
Fragments arranged by size:
2113 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1748 2269 2947 3221 3368 3779 1748
Size: 521 678 274 147 411 2528
Fragments arranged by size:
2528 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1181 1211 1181
Size: 30 4529
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 530 530
Size: 4559
BseYI C'CCAG_C
Cuts at: 1185 1292 1984 2742 3319 3815 1185
Size: 107 692 758 577 496 1929
Fragments arranged by size:
1929 758 692 577 496 107
BsiEI CG_ry'CG
Cuts at: 104 2101 2147 3355 104
Size: 1997 46 1208 1308
Fragments arranged by size:
1997 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1573 2351 2541 3333 1573
Size: 778 190 792 2799
Fragments arranged by size:
2799 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 384 1573 2184 2277 2351 2541 2603 3333 384
Size: 1189 611 93 74 190 62 730 1610
Fragments arranged by size:
1610 1189 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3779 3779
Size: 4559
BspHI T'CATG_A
Cuts at: 3735 3735
Size: 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1609 1823 2125 2506 1609
Size: 214 302 381 3662
Fragments arranged by size:
3662 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2851 2905 4389 4554 50
Size: 2801 54 1484 165 55
Fragments arranged by size:
2801 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2471 2471
Size: 4559
BsrFI r'CCGG_y
Cuts at: 2557 2738 2947 2557
Size: 181 209 4169
Fragments arranged by size:
4169 209 181
BssSI C'ACGA_G
Cuts at: 2830 3188 2830
Size: 358 4201
BstAPI GCAn_nnn'nTGC
Cuts at: 1000 1036 1936 1000
Size: 36 900 3623
Fragments arranged by size:
3623 900 36
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstXI CCAn_nnnn'nTGG
Cuts at: 518 518
Size: 4559
BstYI r'GATC_y
Cuts at: 1 1584 2051 2074 2409 2655 3667 3753 3765
Size: 1583 467 23 335 246 1012 86 12
Cuts at: 3765 4111 1
Size: 346 449
Fragments arranged by size:
1583 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 359 904 359
Size: 545 4014
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2509 2690 2853 2509
Size: 181 163 4215
Fragments arranged by size:
4215 181 163
BtsI GCAGTG_nn'
Cuts at: 1802 1802
Size: 4559
DraI TTT'AAA
Cuts at: 516 1316 1558 516
Size: 800 242 3517
Fragments arranged by size:
3517 800 242
DrdI GACnn_nn'nnGTC
Cuts at: 120 2265 3123 120
Size: 2145 858 1556
Fragments arranged by size:
2145 1556 858
EaeI y'GGCC_r
Cuts at: 2144 2318 2709 2736 4081 2144
Size: 174 391 27 1345 2622
Fragments arranged by size:
2622 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2144 2144
Size: 4559
EarI CTCTTCn'nnn_
Cuts at: 2582 2792 2582
Size: 210 4349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1156 2767 3077 3223 4369 1156
Size: 1611 310 146 1146 1346
Fragments arranged by size:
1611 1346 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1571 1571
Size: 4559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 1061 1089 2384 2816 3563 3831 396
Size: 665 28 1295 432 747 268 1124
Fragments arranged by size:
1295 1124 747 665 432 268 28
EcoO109I rG'GnC_Cy
Cuts at: 1698 1698
Size: 4559
EcoRI G'AATT_C
Cuts at: 1116 1563 1116
Size: 447 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4559
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 1993 2241 3263 4242 1993
Size: 248 1022 979 2310
Fragments arranged by size:
2310 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1034 1066 1146 1178 2401 2431 2433 2463 1034
Size: 32 80 32 1223 30 2 30 3130
Fragments arranged by size:
3130 1223 80 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1598 1598
Size: 4559
KasI G'GCGC_C
Cuts at: 1989 2237 1989
Size: 248 4311
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 732 3230 3414 732
Size: 2498 184 1877
Fragments arranged by size:
2498 1877 184
MscI TGG'CCA
Cuts at: 2320 2320
Size: 4559
MslI CAynn'nnrTG
Cuts at: 516 2548 2675 516
Size: 2032 127 2400
Fragments arranged by size:
2400 2032 127
MspA1I CmG'CkG
Cuts at: 1670 1984 2344 3357 3602 1670
Size: 314 360 1013 245 2627
Fragments arranged by size:
2627 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 1990 2238 1990
Size: 248 4311
NcoI C'CATG_G
Cuts at: 359 904 359
Size: 545 4014
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NspI r_CATG'y
Cuts at: 744 1091 1612 2112 3019 744
Size: 347 521 500 907 2284
Fragments arranged by size:
2284 907 521 500 347
PciI A'CATG_T
Cuts at: 3015 3015
Size: 4559
PflMI CCAn_nnn'nTGG
Cuts at: 1112 1112
Size: 4559
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4527
PsiI TTA'TAA
Cuts at: 1274 1274
Size: 4559
PstI C_TGCA'G
Cuts at: 609 1546 1606 2291 609
Size: 937 60 685 2877
Fragments arranged by size:
2877 937 685 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 1984 2344 1984
Size: 360 4199
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 1573 1573
Size: 4559
SalI G'TCGA_C
Cuts at: 1596 1596
Size: 4559
SapI GCTCTTCn'nnn_
Cuts at: 2582 2792 2582
Size: 210 4349
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 165 4076 165
Size: 3911 648
SfcI C'TryA_G
Cuts at: 33 605 785 1542 1602 2287 3280 3471 33
Size: 572 180 757 60 685 993 191 1121
Fragments arranged by size:
1121 993 757 685 572 191 180 60
SfoI GGC'GCC
Cuts at: 1991 2239 1991
Size: 248 4311
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 1581 1581
Size: 4559
SmlI C'TyrA_G
Cuts at: 1305 1714 2629 3121 3383 1305
Size: 409 915 492 262 2481
Fragments arranged by size:
2481 915 492 409 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 1091 1612 2112 1091
Size: 521 500 3538
Fragments arranged by size:
3538 521 500
SspI AAT'ATT
Cuts at: 1511 1511
Size: 4559
StyI C'CwwG_G
Cuts at: 332 359 904 1340 1693 2047 332
Size: 27 545 436 353 354 2844
Fragments arranged by size:
2844 545 436 354 353 27
SwaI ATTT'AAAT
Cuts at: 516 516
Size: 4559
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 458 687 693 2142 2275 2734 2943 458
Size: 229 6 1449 133 459 209 2074
Fragments arranged by size:
2074 1449 459 229 209 133 6
TatI w'GTAC_w
Cuts at: 163 342 4074 163
Size: 179 3732 648
Fragments arranged by size:
3732 648 179
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 4527
Tth111I GACn'n_nGTC
Cuts at: 2356 2356
Size: 4559
XbaI T'CTAG_A
Cuts at: 1590 1590
Size: 4559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 209 2054 209
Size: 1845 2714
XmaI C'CCGG_G
Cuts at: 1579 1579
Size: 4559
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI XhoI XmnI ZraI