Restriction map for
pF1KB7425
(Circular) (Six-base) MAPSORT of: pF1KB7425.seq Check: 8370 from: 1 to: 4550
pF1KB7425 4550 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 713 1425 1588 713
Size: 712 163 3675
Fragments arranged by size:
3675 712 163
Acc65I G'GTAC_C
Cuts at: 1052 1566 1052
Size: 514 4036
AclI AA'CG_TT
Cuts at: 4390 4390
Size: 4550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 930 1093 1144 1309 1390 2375 2807 3554
Size: 647 163 51 165 81 985 432 747
Cuts at: 3554 3822 283
Size: 268 1011
Fragments arranged by size:
1011 985 747 647 432 268 165 163
81 51
AflIII A'CryG_T
Cuts at: 749 1271 1902 3006 3983 749
Size: 522 631 1104 977 1316
Fragments arranged by size:
1316 1104 977 631 522
AgeI A'CCGG_T
Cuts at: 2938 2938
Size: 4550
AlwNI CAG_nnn'CTG
Cuts at: 156 251 1855 3422 156
Size: 95 1604 1567 1284
Fragments arranged by size:
1604 1567 1284 95
ApaLI G'TGCA_C
Cuts at: 1097 3320 1097
Size: 2223 2327
ApoI r'AATT_y
Cuts at: 438 580 687 740 880 1554 3903 4159 438
Size: 142 107 53 140 674 2349 256 829
Fragments arranged by size:
2349 829 674 256 142 140 107 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 4550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
AvaI C'yCGr_G
Cuts at: 1352 1570 1352
Size: 218 4332
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1044 1077 1044
Size: 33 4517
BamHI G'GATC_C
Cuts at: 1575 1575
Size: 4550
BanI G'GyrC_C
Cuts at: 974 1052 1566 1980 2228 2263 974
Size: 78 514 414 248 35 3261
Fragments arranged by size:
3261 514 414 248 78 35
BanII G_rGCy'C
Cuts at: 212 988 1124 1134 1394 1448 1564 2594 212
Size: 776 136 10 260 54 116 1030 2168
Fragments arranged by size:
2168 1030 776 260 136 116 54 10
BbeI G_GCGC'C
Cuts at: 1984 2232 1984
Size: 248 4302
BbsI GAAGACnn'nnnn_
Cuts at: 325 325
Size: 4550
BciVI GTATCCnnnnn_n'
Cuts at: 736 2448 3209 4550 736
Size: 1712 761 1341 736
Fragments arranged by size:
1712 1341 761 736
BclI T'GATC_A
Cuts at: 968 2070 968
Size: 1102 3448
BglI GCCn_nnn'nGGC
Cuts at: 1310 1310
Size: 4550
BglII A'GATC_T
Cuts at: 335 594 2065 4102 335
Size: 259 1471 2037 783
Fragments arranged by size:
2037 1471 783 259
BlpI GC'TnA_GC
Cuts at: 847 1318 1412 1662 847
Size: 471 94 250 3735
Fragments arranged by size:
3735 471 250 94
Bme1580I G_kGCm'C
Cuts at: 1101 2175 2268 3324 1101
Size: 1074 93 1056 2327
Fragments arranged by size:
2327 1074 1056 93
BmgBI CAC'GTC
Cuts at: 1274 1493 1274
Size: 219 4331
BmrI ACTGGGnnnn_n'
Cuts at: 1762 1843 2178 4322 1762
Size: 81 335 2144 1990
Fragments arranged by size:
2144 1990 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 352 479 898 925 1531 157
Size: 195 127 419 27 606 3176
Fragments arranged by size:
3176 606 419 195 127 27
Bpu10I CC'TnA_GC
Cuts at: 790 1225 790
Size: 435 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1138 1726 2605 3097 3395 556
Size: 582 588 879 492 298 1711
Fragments arranged by size:
1711 879 588 582 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4254 4254
Size: 4550
BsaAI yAC'GTr
Cuts at: 1903 1903
Size: 4550
BsaBI GATnn'nnATC
Cuts at: 6 593 2064 4101 6
Size: 587 1471 2037 455
Fragments arranged by size:
2037 1471 587 455
BsaHI Gr'CG_yC
Cuts at: 322 1981 2229 2931 4427 322
Size: 1659 248 702 1496 445
Fragments arranged by size:
1659 1496 702 445 248
BsaWI w'CCGG_w
Cuts at: 1467 1739 2260 2938 3212 3359 3770 1467
Size: 272 521 678 274 147 411 2247
Fragments arranged by size:
2247 678 521 411 274 272 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 116 146 116
Size: 30 4520
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 886 1384 886
Size: 498 4052
BseYI C'CCAG_C
Cuts at: 159 1975 2733 3310 3806 159
Size: 1816 758 577 496 903
Fragments arranged by size:
1816 903 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 295
Size: 4550
BsiEI CG_ry'CG
Cuts at: 104 2092 2138 3346 104
Size: 1988 46 1208 1308
Fragments arranged by size:
1988 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 212 896 1101 1134 1394 1564 2342 2532 3324
Size: 684 205 33 260 170 778 190 792
Cuts at: 3324 212
Size: 1438
Fragments arranged by size:
1438 792 778 684 260 205 190 170
33
BsmI GAATG_Cn'
Cuts at: 680 680
Size: 4550
Bsp1286I G_dGCh'C
Cuts at: 212 896 988 1101 1124 1134 1394 1448 1564
Size: 684 92 113 23 10 260 54 116
Cuts at: 1564 2175 2268 2342 2532 2594 3324 212
Size: 611 93 74 190 62 730 1438
Fragments arranged by size:
1438 730 684 611 260 190 116 113
93 92 74 62 54 23 10
BspEI T'CCGG_A
Cuts at: 3770 3770
Size: 4550
BspHI T'CATG_A
Cuts at: 3726 3726
Size: 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 851 1304 1600 1814 2116 2497 851
Size: 453 296 214 302 381 2904
Fragments arranged by size:
2904 453 381 302 296 214
BsrBI CCG'CTC
Cuts at: 50 2842 2896 4380 4545 50
Size: 2792 54 1484 165 55
Fragments arranged by size:
2792 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1272 2462 1272
Size: 1190 3360
BsrFI r'CCGG_y
Cuts at: 2548 2729 2938 2548
Size: 181 209 4160
Fragments arranged by size:
4160 209 181
BssSI C'ACGA_G
Cuts at: 2821 3179 2821
Size: 358 4192
BstAPI GCAn_nnn'nTGC
Cuts at: 156 251 1927 156
Size: 95 1676 2779
Fragments arranged by size:
2779 1676 95
BstBI TT'CG_AA
Cuts at: 2911 2911
Size: 4550
BstYI r'GATC_y
Cuts at: 1 335 594 1454 1575 2042 2065 2400 2646
Size: 334 259 860 121 467 23 335 246
Cuts at: 2646 3658 3744 3756 4102 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 860 467 449 346 335 334 259
246 121 86 23 12
BstZ17I GTA'TAC
Cuts at: 714 1426 714
Size: 712 3838
Bsu36I CC'TnA_GG
Cuts at: 898 979 898
Size: 81 4469
BtgI C'CryG_G
Cuts at: 1277 1277
Size: 4550
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2500 2681 2844 2500
Size: 181 163 4206
Fragments arranged by size:
4206 181 163
BtsI GCAGTG_nn'
Cuts at: 1099 1793 1099
Size: 694 3856
DraI TTT'AAA
Cuts at: 1549 1549
Size: 4550
DrdI GACnn_nn'nnGTC
Cuts at: 453 2256 3114 453
Size: 1803 858 1889
Fragments arranged by size:
1889 1803 858
EaeI y'GGCC_r
Cuts at: 176 757 2135 2309 2700 2727 4072 176
Size: 581 1378 174 391 27 1345 654
Fragments arranged by size:
1378 1345 654 581 391 174 27
EagI C'GGCC_G
Cuts at: 2135 2135
Size: 4550
EarI CTCTTCn'nnn_
Cuts at: 217 624 823 1163 1174 2573 2783 217
Size: 407 199 340 11 1399 210 1984
Fragments arranged by size:
1984 1399 407 340 210 199 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 414 2758 3068 3214 4360 414
Size: 2344 310 146 1146 604
Fragments arranged by size:
2344 1146 604 310 146
EcoICRI GAG'CTC
Cuts at: 210 1132 1392 1562 210
Size: 922 260 170 3198
Fragments arranged by size:
3198 922 260 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 283 352 479 898 925 930 1093 1144
Size: 126 69 127 419 27 5 163 51
Cuts at: 1144 1309 1390 1531 2375 2807 3554 3822 157
Size: 165 81 141 844 432 747 268 885
Fragments arranged by size:
885 844 747 432 419 268 165 163
141 127 126 81 69 51 27 5
EcoO109I rG'GnC_Cy
Cuts at: 120 308 467 1293 1689 120
Size: 188 159 826 396 2981
Fragments arranged by size:
2981 826 396 188 159
EcoRI G'AATT_C
Cuts at: 880 1554 880
Size: 674 3876
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 561 2569 561
Size: 2008 2542
FspI TGC'GCA
Cuts at: 2331 2331
Size: 4550
HaeII r_GCGC'y
Cuts at: 1984 2232 3254 4233 1984
Size: 248 1022 979 2301
Fragments arranged by size:
2301 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 312 344 789 821 2392 2422 2424 2454 312
Size: 32 445 32 1571 30 2 30 2408
Fragments arranged by size:
2408 1571 445 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1069 1589 1069
Size: 520 4030
KasI G'GCGC_C
Cuts at: 1980 2228 1980
Size: 248 4302
KpnI G_GTAC'C
Cuts at: 1056 1570 1056
Size: 514 4036
MluI A'CGCG_T
Cuts at: 3983 3983
Size: 4550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 716 1262 3221 3405 716
Size: 546 1959 184 1861
Fragments arranged by size:
1959 1861 546 184
MscI TGG'CCA
Cuts at: 178 2311 178
Size: 2133 2417
MslI CAynn'nnrTG
Cuts at: 1265 1276 2539 2666 1265
Size: 11 1263 127 3149
Fragments arranged by size:
3149 1263 127 11
MspA1I CmG'CkG
Cuts at: 966 1080 1661 1975 2335 3348 3593 966
Size: 114 581 314 360 1013 245 1923
Fragments arranged by size:
1923 1013 581 360 314 245 114
NaeI GCC'GGC
Cuts at: 2731 2731
Size: 4550
NarI GG'CG_CC
Cuts at: 1981 2229 1981
Size: 248 4302
NcoI C'CATG_G
Cuts at: 1277 1277
Size: 4550
NdeI CA'TA_TG
Cuts at: 451 451
Size: 4550
NgoMIV G'CCGG_C
Cuts at: 2729 2729
Size: 4550
NspI r_CATG'y
Cuts at: 753 1603 2103 3010 753
Size: 850 500 907 2293
Fragments arranged by size:
2293 907 850 500
PasI CC'CwG_GG
Cuts at: 203 203
Size: 4550
PciI A'CATG_T
Cuts at: 749 3006 749
Size: 2257 2293
PflMI CCAn_nnn'nTGG
Cuts at: 174 735 174
Size: 561 3989
PfoI T'CCnGG_A
Cuts at: 134 134
Size: 4550
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 4518
PpuMI rG'GwC_Cy
Cuts at: 308 1293 308
Size: 985 3565
PshAI GACnn'nnGTC
Cuts at: 1373 1373
Size: 4550
PstI C_TGCA'G
Cuts at: 1597 2282 1597
Size: 685 3865
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4550
PvuII CAG'CTG
Cuts at: 966 1080 1975 2335 966
Size: 114 895 360 3181
Fragments arranged by size:
3181 895 360 114
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4550
SacI G_AGCT'C
Cuts at: 212 1134 1394 1564 212
Size: 922 260 170 3198
Fragments arranged by size:
3198 922 260 170
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4550
SanDI GG'GwC_CC
Cuts at: 308 308
Size: 4550
SapI GCTCTTCn'nnn_
Cuts at: 217 2573 2783 217
Size: 2356 210 1984
Fragments arranged by size:
2356 1984 210
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4550
ScaI AGT'ACT
Cuts at: 4067 4067
Size: 4550
SfcI C'TryA_G
Cuts at: 33 115 1593 2278 3271 3462 33
Size: 82 1478 685 993 191 1121
Fragments arranged by size:
1478 1121 993 685 191 82
SfoI GGC'GCC
Cuts at: 1982 2230 1982
Size: 248 4302
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
SmaI CCC'GGG
Cuts at: 1572 1572
Size: 4550
SmlI C'TyrA_G
Cuts at: 571 1117 1705 2620 3112 3374 571
Size: 546 588 915 492 262 1747
Fragments arranged by size:
1747 915 588 546 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4550
SphI G_CATG'C
Cuts at: 1603 2103 1603
Size: 500 4050
StuI AGG'CCT
Cuts at: 172 235 280 1539 172
Size: 63 45 1259 3183
Fragments arranged by size:
3183 1259 63 45
StyI C'CwwG_G
Cuts at: 173 523 1277 1684 2038 173
Size: 350 754 407 354 2685
Fragments arranged by size:
2685 754 407 354 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 602 2133 2266 2725 2934 602
Size: 1531 133 459 209 2218
Fragments arranged by size:
2218 1531 459 209 133
TatI w'GTAC_w
Cuts at: 639 652 4065 639
Size: 13 3413 1124
Fragments arranged by size:
3413 1124 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 116 148 4067 4099 116
Size: 32 3919 32 567
Fragments arranged by size:
3919 567 32 32
Tth111I GACn'n_nGTC
Cuts at: 2347 2347
Size: 4550
XbaI T'CTAG_A
Cuts at: 1581 1581
Size: 4550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 186 1506 2045 186
Size: 1320 539 2691
Fragments arranged by size:
2691 1320 539
XmaI C'CCGG_G
Cuts at: 1570 1570
Size: 4550
XmnI GAAnn'nnTTC
Cuts at: 553 633 553
Size: 80 4470
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BfrBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstXI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PmlI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI