Restriction map for
pF1KB7426
(Circular) (Six-base) MAPSORT of: pF1KB7426.seq Check: 9255 from: 1 to: 4349
pF1KB7426 4349 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1387 1387
Size: 4349
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2606 3353 3621 2174
Size: 432 747 268 2902
Fragments arranged by size:
2902 747 432 268
AfeI AGC'GCT
Cuts at: 514 1323 514
Size: 809 3540
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AlwNI CAG_nnn'CTG
Cuts at: 119 1654 3221 119
Size: 1535 1567 1247
Fragments arranged by size:
1567 1535 1247
ApaI G_GGCC'C
Cuts at: 773 773
Size: 4349
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 1353 3702 3958 1353
Size: 2349 256 1744
Fragments arranged by size:
2349 1744 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 139 373 672 772 966 1369 139
Size: 234 299 100 194 403 3119
Fragments arranged by size:
3119 403 299 234 194 100
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 222 563 685 744 1365 1779 2027 2062 222
Size: 341 122 59 621 414 248 35 2509
Fragments arranged by size:
2509 621 414 341 248 122 59 35
BanII G_rGCy'C
Cuts at: 773 823 914 1363 2393 773
Size: 50 91 449 1030 2729
Fragments arranged by size:
2729 1030 449 91 50
BbeI G_GCGC'C
Cuts at: 567 748 1783 2031 567
Size: 181 1035 248 2885
Fragments arranged by size:
2885 1035 248 181
BbsI GAAGACnn'nnnn_
Cuts at: 1339 1339
Size: 4349
BbvCI CC'TCA_GC
Cuts at: 895 895
Size: 4349
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglI GCCn_nnn'nGGC
Cuts at: 162 683 732 768 162
Size: 521 49 36 3743
Fragments arranged by size:
3743 521 49 36
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 502 1461 502
Size: 959 3390
Bme1580I G_kGCm'C
Cuts at: 773 1974 2067 3123 773
Size: 1201 93 1056 1999
Fragments arranged by size:
1999 1201 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1561 1642 1977 4121 1561
Size: 81 335 2144 1789
Fragments arranged by size:
2144 1789 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 4349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1310 1310
Size: 4349
Bpu10I CC'TnA_GC
Cuts at: 895 895
Size: 4349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1525 2404 2896 3194 1525
Size: 879 492 298 2680
Fragments arranged by size:
2680 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 769 4053 769
Size: 3284 1065
BsaAI yAC'GTr
Cuts at: 456 1702 456
Size: 1246 3103
BsaBI GATnn'nnATC
Cuts at: 6 292 1863 3900 6
Size: 286 1571 2037 455
Fragments arranged by size:
2037 1571 455 286
BsaHI Gr'CG_yC
Cuts at: 564 641 745 1780 2028 2730 4226 564
Size: 77 104 1035 248 702 1496 687
Fragments arranged by size:
1496 1035 702 687 248 104 77
BsaWI w'CCGG_w
Cuts at: 1538 2059 2737 3011 3158 3569 1538
Size: 521 678 274 147 411 2318
Fragments arranged by size:
2318 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 1286 1316 145
Size: 30 1111 30 3178
Fragments arranged by size:
3178 1111 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 693 1307 693
Size: 614 3735
BseYI C'CCAG_C
Cuts at: 831 1774 2532 3109 3605 831
Size: 943 758 577 496 1575
Fragments arranged by size:
1575 943 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 472 453
Size: 19 4330
BsiEI CG_ry'CG
Cuts at: 104 817 946 962 1891 1937 3145 104
Size: 713 129 16 929 46 1208 1308
Fragments arranged by size:
1308 1208 929 713 129 46 16
BsiHKAI G_wGCw'C
Cuts at: 593 1363 2141 2331 3123 593
Size: 770 778 190 792 1819
Fragments arranged by size:
1819 792 778 770 190
BsmI GAATG_Cn'
Cuts at: 814 814
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 593 773 823 914 1363 1974 2067 2141 2331
Size: 180 50 91 449 611 93 74 190
Cuts at: 2331 2393 3123 593
Size: 62 730 1819
Fragments arranged by size:
1819 730 611 449 190 180 93 91
74 62 50
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1399 1613 1915 2296 1399
Size: 214 302 381 3452
Fragments arranged by size:
3452 381 302 214
BsrBI CCG'CTC
Cuts at: 50 269 537 1198 2641 2695 4179 4344 50
Size: 219 268 661 1443 54 1484 165 55
Fragments arranged by size:
1484 1443 661 268 219 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2261 2261
Size: 4349
BsrFI r'CCGG_y
Cuts at: 225 616 812 938 986 2347 2528 2737 225
Size: 391 196 126 48 1361 181 209 1837
Fragments arranged by size:
1837 1361 391 209 196 181 126 48
BssHII G'CGCG_C
Cuts at: 575 836 575
Size: 261 4088
BssSI C'ACGA_G
Cuts at: 2620 2978 2620
Size: 358 3991
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstEII G'GTnAC_C
Cuts at: 228 279 555 858 228
Size: 51 276 303 3719
Fragments arranged by size:
3719 303 276 51
BstYI r'GATC_y
Cuts at: 1 1374 1841 1864 2199 2445 3457 3543 3555
Size: 1373 467 23 335 246 1012 86 12
Cuts at: 3555 3901 1
Size: 346 449
Fragments arranged by size:
1373 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 160 599 779 788 107
Size: 53 439 180 9 3668
Fragments arranged by size:
3668 439 180 53 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 567 2299 2480 2643 567
Size: 1732 181 163 2273
Fragments arranged by size:
2273 1732 181 163
BtsI GCAGTG_nn'
Cuts at: 1262 1592 1262
Size: 330 4019
DraI TTT'AAA
Cuts at: 1209 1348 1209
Size: 139 4210
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 347 521 814 943 959 1934 2108 2499 2526
Size: 174 293 129 16 975 174 391 27
Cuts at: 2526 3871 347
Size: 1345 825
Fragments arranged by size:
1345 975 825 391 293 174 174 129
27 16
EagI C'GGCC_G
Cuts at: 814 943 959 1934 814
Size: 129 16 975 3229
Fragments arranged by size:
3229 975 129 16
EarI CTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2557 2867 3013 4159 2557
Size: 310 146 1146 2747
Fragments arranged by size:
2747 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1361 1361
Size: 4349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1310 2174 2606 3353 3621 1310
Size: 864 432 747 268 2038
Fragments arranged by size:
2038 864 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 651 769 1488 651
Size: 118 719 3512
Fragments arranged by size:
3512 719 118
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4349
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 192 220 303 408 516 567 607 748 796
Size: 28 83 105 108 51 40 141 48
Cuts at: 796 1325 1783 2031 3053 4032 192
Size: 529 458 248 1022 979 509
Fragments arranged by size:
1022 979 529 509 458 248 141 108
105 83 51 48 40 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2191 2221 2223 2253 2191
Size: 30 2 30 4287
Fragments arranged by size:
4287 30 30 2
HincII GTy'rAC
Cuts at: 1388 1388
Size: 4349
KasI G'GCGC_C
Cuts at: 563 744 1779 2027 563
Size: 181 1035 248 2885
Fragments arranged by size:
2885 1035 248 181
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 380 3020 3204 380
Size: 2640 184 1525
Fragments arranged by size:
2640 1525 184
MscI TGG'CCA
Cuts at: 523 2110 523
Size: 1587 2762
MslI CAynn'nnrTG
Cuts at: 349 586 2338 2465 349
Size: 237 1752 127 2233
Fragments arranged by size:
2233 1752 237 127
MspA1I CmG'CkG
Cuts at: 194 235 432 781 790 831 948 1460 1774
Size: 41 197 349 9 41 117 512 314
Cuts at: 1774 2134 3147 3392 194
Size: 360 1013 245 1151
Fragments arranged by size:
1151 1013 512 360 349 314 245 197
117 41 41 9
NaeI GCC'GGC
Cuts at: 814 940 2530 814
Size: 126 1590 2633
Fragments arranged by size:
2633 1590 126
NarI GG'CG_CC
Cuts at: 564 745 1780 2028 564
Size: 181 1035 248 2885
Fragments arranged by size:
2885 1035 248 181
NcoI C'CATG_G
Cuts at: 107 107
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 812 938 2528 812
Size: 126 1590 2633
Fragments arranged by size:
2633 1590 126
NotI GC'GGCC_GC
Cuts at: 943 943
Size: 4349
NspI r_CATG'y
Cuts at: 315 1402 1902 2809 315
Size: 1087 500 907 1855
Fragments arranged by size:
1855 1087 907 500
PasI CC'CwG_GG
Cuts at: 210 416 210
Size: 206 4143
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PpuMI rG'GwC_Cy
Cuts at: 651 651
Size: 4349
PsiI TTA'TAA
Cuts at: 1030 1030
Size: 4349
PspOMI G'GGCC_C
Cuts at: 769 769
Size: 4349
PstI C_TGCA'G
Cuts at: 1396 2081 1396
Size: 685 3664
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 831 1774 2134 831
Size: 943 360 3046
Fragments arranged by size:
3046 943 360
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 1363 1363
Size: 4349
SacII CC_GC'GG
Cuts at: 782 791 782
Size: 9 4340
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 1111 3866 1111
Size: 2755 1594
SfcI C'TryA_G
Cuts at: 33 1392 2077 3070 3261 33
Size: 1359 685 993 191 1121
Fragments arranged by size:
1359 1121 993 685 191
SfoI GGC'GCC
Cuts at: 565 746 1781 2029 565
Size: 181 1035 248 2885
Fragments arranged by size:
2885 1035 248 181
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 674 1371 674
Size: 697 3652
SmlI C'TyrA_G
Cuts at: 1504 2419 2911 3173 1504
Size: 915 492 262 2680
Fragments arranged by size:
2680 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StyI C'CwwG_G
Cuts at: 107 1483 1837 107
Size: 1376 354 2619
Fragments arranged by size:
2619 1376 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 408 1932 2065 2524 2733 408
Size: 1524 133 459 209 2024
Fragments arranged by size:
2024 1524 459 209 133
TatI w'GTAC_w
Cuts at: 1097 1109 1190 3864 1097
Size: 12 81 2674 1582
Fragments arranged by size:
2674 1582 81 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 145 177 1286 1318 3866 3898 145
Size: 32 1109 32 2548 32 596
Fragments arranged by size:
2548 1109 596 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 333 1844 333
Size: 1511 2838
XmaI C'CCGG_G
Cuts at: 672 1369 672
Size: 697 3652
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PciI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PflMI PfoI PmlI PshAI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI