Restriction map for
pF1KB7427
(Circular) (Six-base) MAPSORT of: pF1KB7427.seq Check: 6617 from: 1 to: 3569
pF1KB7427 3569 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 607 607
Size: 3569
Acc65I G'GTAC_C
Cuts at: 379 585 379
Size: 206 3363
AclI AA'CG_TT
Cuts at: 3409 3409
Size: 3569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1394 1826 2573 2841 511
Size: 883 432 747 268 1239
Fragments arranged by size:
1239 883 747 432 268
AflIII A'CryG_T
Cuts at: 921 2025 3002 921
Size: 1104 977 1488
Fragments arranged by size:
1488 1104 977
AgeI A'CCGG_T
Cuts at: 1957 1957
Size: 3569
AlwNI CAG_nnn'CTG
Cuts at: 874 2441 874
Size: 1567 2002
ApaLI G'TGCA_C
Cuts at: 2339 2339
Size: 3569
ApoI r'AATT_y
Cuts at: 334 573 2922 3178 334
Size: 239 2349 256 725
Fragments arranged by size:
2349 725 256 239
AseI AT'TA_AT
Cuts at: 20 448 20
Size: 428 3141
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3569
AvaI C'yCGr_G
Cuts at: 589 589
Size: 3569
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 363 396 363
Size: 33 3536
BamHI G'GATC_C
Cuts at: 594 594
Size: 3569
BanI G'GyrC_C
Cuts at: 379 585 999 1247 1282 379
Size: 206 414 248 35 2666
Fragments arranged by size:
2666 414 248 206 35
BanII G_rGCy'C
Cuts at: 583 1613 583
Size: 1030 2539
BbeI G_GCGC'C
Cuts at: 1003 1251 1003
Size: 248 3321
BbsI GAAGACnn'nnnn_
Cuts at: 278 278
Size: 3569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3535
BciVI GTATCCnnnnn_n'
Cuts at: 1467 2228 3569 1467
Size: 761 1341 1467
Fragments arranged by size:
1467 1341 761
BclI T'GATC_A
Cuts at: 1089 1089
Size: 3569
BglII A'GATC_T
Cuts at: 1084 3121 1084
Size: 2037 1532
BlpI GC'TnA_GC
Cuts at: 681 681
Size: 3569
Bme1580I G_kGCm'C
Cuts at: 1194 1287 2343 1194
Size: 93 1056 2420
Fragments arranged by size:
2420 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 781 862 1197 3341 781
Size: 81 335 2144 1009
Fragments arranged by size:
2144 1009 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1936 1936
Size: 3569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 1624 2116 2414 745
Size: 879 492 298 1900
Fragments arranged by size:
1900 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 380 3273 380
Size: 2893 676
BsaAI yAC'GTr
Cuts at: 922 922
Size: 3569
BsaBI GATnn'nnATC
Cuts at: 6 1083 3120 6
Size: 1077 2037 455
Fragments arranged by size:
2037 1077 455
BsaHI Gr'CG_yC
Cuts at: 1000 1248 1950 3446 1000
Size: 248 702 1496 1123
Fragments arranged by size:
1496 1123 702 248
BsaWI w'CCGG_w
Cuts at: 758 1279 1957 2231 2378 2789 758
Size: 521 678 274 147 411 1538
Fragments arranged by size:
1538 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 994 1752 2329 2825 994
Size: 758 577 496 1738
Fragments arranged by size:
1738 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1111 1157 2365 104
Size: 1007 46 1208 1308
Fragments arranged by size:
1308 1208 1007 46
BsiHKAI G_wGCw'C
Cuts at: 583 1361 1551 2343 583
Size: 778 190 792 1809
Fragments arranged by size:
1809 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 583 1194 1287 1361 1551 1613 2343 583
Size: 611 93 74 190 62 730 1809
Fragments arranged by size:
1809 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2789 2789
Size: 3569
BspHI T'CATG_A
Cuts at: 2745 2745
Size: 3569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 619 833 1135 1516 619
Size: 214 302 381 2672
Fragments arranged by size:
2672 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1861 1915 3399 3564 50
Size: 1811 54 1484 165 55
Fragments arranged by size:
1811 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1481 1481
Size: 3569
BsrFI r'CCGG_y
Cuts at: 1567 1748 1957 1567
Size: 181 209 3179
Fragments arranged by size:
3179 209 181
BssSI C'ACGA_G
Cuts at: 1840 2198 1840
Size: 358 3211
BstAPI GCAn_nnn'nTGC
Cuts at: 946 946
Size: 3569
BstBI TT'CG_AA
Cuts at: 213 1930 213
Size: 1717 1852
BstEII G'GTnAC_C
Cuts at: 134 134
Size: 3569
BstYI r'GATC_y
Cuts at: 1 594 1061 1084 1419 1665 2677 2763 2775
Size: 593 467 23 335 246 1012 86 12
Cuts at: 2775 3121 1
Size: 346 449
Fragments arranged by size:
1012 593 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1519 1700 1863 1519
Size: 181 163 3225
Fragments arranged by size:
3225 181 163
BtsI GCAGTG_nn'
Cuts at: 812 812
Size: 3569
DraI TTT'AAA
Cuts at: 568 568
Size: 3569
DrdI GACnn_nn'nnGTC
Cuts at: 1275 2133 1275
Size: 858 2711
EaeI y'GGCC_r
Cuts at: 464 1154 1328 1719 1746 3091 464
Size: 690 174 391 27 1345 942
Fragments arranged by size:
1345 942 690 391 174 27
EagI C'GGCC_G
Cuts at: 1154 1154
Size: 3569
EarI CTCTTCn'nnn_
Cuts at: 1592 1802 1592
Size: 210 3359
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1777 2087 2233 3379 1777
Size: 310 146 1146 1967
Fragments arranged by size:
1967 1146 310 146
EcoICRI GAG'CTC
Cuts at: 581 581
Size: 3569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1394 1826 2573 2841 511
Size: 883 432 747 268 1239
Fragments arranged by size:
1239 883 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 708 708
Size: 3569
EcoRI G'AATT_C
Cuts at: 334 573 334
Size: 239 3330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1588 1588
Size: 3569
FspI TGC'GCA
Cuts at: 1350 1350
Size: 3569
HaeII r_GCGC'y
Cuts at: 1003 1251 2273 3252 1003
Size: 248 1022 979 1320
Fragments arranged by size:
1320 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 377 379 409 411 1411 1441 1443 1473 377
Size: 2 30 2 1000 30 2 30 2473
Fragments arranged by size:
2473 1000 30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 608 608
Size: 3569
KasI G'GCGC_C
Cuts at: 999 1247 999
Size: 248 3321
KpnI G_GTAC'C
Cuts at: 383 589 383
Size: 206 3363
MluI A'CGCG_T
Cuts at: 3002 3002
Size: 3569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2240 2424 2240
Size: 184 3385
MscI TGG'CCA
Cuts at: 466 1330 466
Size: 864 2705
MslI CAynn'nnrTG
Cuts at: 112 423 1558 1685 112
Size: 311 1135 127 1996
Fragments arranged by size:
1996 1135 311 127
MspA1I CmG'CkG
Cuts at: 680 994 1354 2367 2612 680
Size: 314 360 1013 245 1637
Fragments arranged by size:
1637 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1750 1750
Size: 3569
NarI GG'CG_CC
Cuts at: 1000 1248 1000
Size: 248 3321
NgoMIV G'CCGG_C
Cuts at: 1748 1748
Size: 3569
NspI r_CATG'y
Cuts at: 622 1122 2029 622
Size: 500 907 2162
Fragments arranged by size:
2162 907 500
PciI A'CATG_T
Cuts at: 2025 2025
Size: 3569
PmeI GTTT'AAAC
Cuts at: 568 568
Size: 3569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 404 436 2708 2740 404
Size: 32 2272 32 1233
Fragments arranged by size:
2272 1233 32 32
PsiI TTA'TAA
Cuts at: 506 506
Size: 3569
PstI C_TGCA'G
Cuts at: 616 1301 616
Size: 685 2884
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3569
PvuII CAG'CTG
Cuts at: 994 1354 994
Size: 360 3209
RsrII CG'GwC_CG
Cuts at: 1764 1764
Size: 3569
SacI G_AGCT'C
Cuts at: 583 583
Size: 3569
SalI G'TCGA_C
Cuts at: 606 606
Size: 3569
SapI GCTCTTCn'nnn_
Cuts at: 1592 1802 1592
Size: 210 3359
SbfI CC_TGCA'GG
Cuts at: 616 616
Size: 3569
ScaI AGT'ACT
Cuts at: 3086 3086
Size: 3569
SfcI C'TryA_G
Cuts at: 33 129 612 1297 2290 2481 33
Size: 96 483 685 993 191 1121
Fragments arranged by size:
1121 993 685 483 191 96
SfoI GGC'GCC
Cuts at: 1001 1249 1001
Size: 248 3321
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3569
SmaI CCC'GGG
Cuts at: 591 591
Size: 3569
SmlI C'TyrA_G
Cuts at: 724 1639 2131 2393 724
Size: 915 492 262 1900
Fragments arranged by size:
1900 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3569
SphI G_CATG'C
Cuts at: 622 1122 622
Size: 500 3069
SspI AAT'ATT
Cuts at: 218 405 218
Size: 187 3382
StyI C'CwwG_G
Cuts at: 467 703 1057 467
Size: 236 354 2979
Fragments arranged by size:
2979 354 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1152 1285 1744 1953 1152
Size: 133 459 209 2768
Fragments arranged by size:
2768 459 209 133
TatI w'GTAC_w
Cuts at: 3084 3084
Size: 3569
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 3537
Tth111I GACn'n_nGTC
Cuts at: 159 1366 159
Size: 1207 2362
XbaI T'CTAG_A
Cuts at: 201 600 201
Size: 399 3170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1064 1064
Size: 3569
XmaI C'CCGG_G
Cuts at: 589 589
Size: 3569
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI