Restriction map for
pF1KB7428
(Circular) (Six-base) MAPSORT of: pF1KB7428.seq Check: 3656 from: 1 to: 4349
pF1KB7428 4349 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 525 525
Size: 4349
AccI GT'mk_AC
Cuts at: 1238 1387 1238
Size: 149 4200
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 2174 2606 3353 3621 296
Size: 1878 432 747 268 1024
Fragments arranged by size:
1878 1024 747 432 268
AfeI AGC'GCT
Cuts at: 725 725
Size: 4349
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AlwNI CAG_nnn'CTG
Cuts at: 1654 3221 1654
Size: 1567 2782
ApaI G_GGCC'C
Cuts at: 348 355 1325 348
Size: 7 970 3372
Fragments arranged by size:
3372 970 7
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 460 983 1353 3702 3958 460
Size: 523 370 2349 256 851
Fragments arranged by size:
2349 851 523 370 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 719 833 1369 719
Size: 114 536 3699
Fragments arranged by size:
3699 536 114
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 1047 1365 1779 2027 2062 1047
Size: 318 414 248 35 3334
Fragments arranged by size:
3334 414 318 248 35
BanII G_rGCy'C
Cuts at: 348 355 437 746 1061 1325 1363 2393 348
Size: 7 82 309 315 264 38 1030 2304
Fragments arranged by size:
2304 1030 315 309 264 82 38 7
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbvCI CC'TCA_GC
Cuts at: 257 326 634 257
Size: 69 308 3972
Fragments arranged by size:
3972 308 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 118 152 519 553 118
Size: 34 367 34 3914
Fragments arranged by size:
3914 367 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BfrBI ATG'CAT
Cuts at: 937 937
Size: 4349
BglI GCCn_nnn'nGGC
Cuts at: 112 877 112
Size: 765 3584
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 727 944 1461 727
Size: 217 517 3615
Fragments arranged by size:
3615 517 217
Bme1580I G_kGCm'C
Cuts at: 348 355 521 1325 1974 2067 3123 348
Size: 7 166 804 649 93 1056 1574
Fragments arranged by size:
1574 1056 804 649 166 93 7
BmrI ACTGGGnnnn_n'
Cuts at: 675 879 1033 1115 1561 1642 1977 4121 675
Size: 204 154 82 446 81 335 2144 903
Fragments arranged by size:
2144 903 446 335 204 154 82 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 4349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 4349
Bpu10I CC'TnA_GC
Cuts at: 257 326 634 842 257
Size: 69 308 208 3764
Fragments arranged by size:
3764 308 208 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1525 2404 2896 3194 1525
Size: 879 492 298 2680
Fragments arranged by size:
2680 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4053 4053
Size: 4349
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 502 1780 2028 2730 4226 502
Size: 1278 248 702 1496 625
Fragments arranged by size:
1496 1278 702 625 248
BsaWI w'CCGG_w
Cuts at: 212 1538 2059 2737 3011 3158 3569 212
Size: 1326 521 678 274 147 411 992
Fragments arranged by size:
1326 992 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 786 816 786
Size: 30 4319
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 328 331 343 988 1069 301
Size: 27 3 12 645 81 3581
Fragments arranged by size:
3581 645 81 27 12 3
BseYI C'CCAG_C
Cuts at: 355 849 900 1774 2532 3109 3605 355
Size: 494 51 874 758 577 496 1099
Fragments arranged by size:
1099 874 758 577 496 494 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1286 1286
Size: 4349
BsiEI CG_ry'CG
Cuts at: 104 662 1891 1937 3145 104
Size: 558 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 558 46
BsiHKAI G_wGCw'C
Cuts at: 437 542 746 1363 2141 2331 3123 437
Size: 105 204 617 778 190 792 1663
Fragments arranged by size:
1663 792 778 617 204 190 105
BsiWI C'GTAC_G
Cuts at: 704 704
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 348 355 437 521 542 746 1061 1325 1363
Size: 7 82 84 21 204 315 264 38
Cuts at: 1363 1974 2067 2141 2331 2393 3123 348
Size: 611 93 74 190 62 730 1574
Fragments arranged by size:
1574 730 611 315 264 204 190 93
84 82 74 62 38 21 7
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 525 1399 1613 1915 2296 525
Size: 874 214 302 381 2578
Fragments arranged by size:
2578 874 381 302 214
BsrBI CCG'CTC
Cuts at: 50 807 1161 2641 2695 4179 4344 50
Size: 757 354 1480 54 1484 165 55
Fragments arranged by size:
1484 1480 757 354 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2261 2261
Size: 4349
BsrFI r'CCGG_y
Cuts at: 2347 2528 2737 2347
Size: 181 209 3959
Fragments arranged by size:
3959 209 181
BssSI C'ACGA_G
Cuts at: 394 2620 2978 394
Size: 2226 358 1765
Fragments arranged by size:
2226 1765 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstEII G'GTnAC_C
Cuts at: 567 567
Size: 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 379 379
Size: 4349
BstYI r'GATC_y
Cuts at: 1 1374 1841 1864 2199 2445 3457 3543 3555
Size: 1373 467 23 335 246 1012 86 12
Cuts at: 3555 3901 1
Size: 346 449
Fragments arranged by size:
1373 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 230 1150 230
Size: 920 3429
BtgI C'CryG_G
Cuts at: 107 1134 107
Size: 1027 3322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2299 2480 2643 2299
Size: 181 163 4005
Fragments arranged by size:
4005 181 163
BtsI GCAGTG_nn'
Cuts at: 1003 1197 1592 1003
Size: 194 395 3760
Fragments arranged by size:
3760 395 194
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 659 1934 2108 2499 2526 3871 659
Size: 1275 174 391 27 1345 1137
Fragments arranged by size:
1345 1275 1137 391 174 27
EagI C'GGCC_G
Cuts at: 659 1934 659
Size: 1275 3074
EarI CTCTTCn'nnn_
Cuts at: 257 814 1063 1151 2372 2582 257
Size: 557 249 88 1221 210 2024
Fragments arranged by size:
2024 1221 557 249 210 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2557 2867 3013 4159 2557
Size: 310 146 1146 2747
Fragments arranged by size:
2747 1146 310 146
EcoICRI GAG'CTC
Cuts at: 435 744 1361 435
Size: 309 617 3423
Fragments arranged by size:
3423 617 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 730 2174 2606 3353 3621 296
Size: 434 1444 432 747 268 1024
Fragments arranged by size:
1444 1024 747 434 432 268
EcoO109I rG'GnC_Cy
Cuts at: 114 225 236 344 351 352 846 859 1027
Size: 111 11 108 7 1 494 13 168
Cuts at: 1027 1087 1321 1322 1488 114
Size: 60 234 1 166 2975
Fragments arranged by size:
2975 494 234 168 166 111 108 60
13 11 7 1 1
EcoRI G'AATT_C
Cuts at: 983 1353 983
Size: 370 3979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4349
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 727 842 1783 2031 3053 4032 727
Size: 115 941 248 1022 979 1044
Fragments arranged by size:
1044 1022 979 941 248 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2191 2221 2223 2253 2191
Size: 30 2 30 4287
Fragments arranged by size:
4287 30 30 2
HincII GTy'rAC
Cuts at: 1388 1388
Size: 4349
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1315 3020 3204 1315
Size: 1705 184 2460
Fragments arranged by size:
2460 1705 184
MscI TGG'CCA
Cuts at: 2110 2110
Size: 4349
MslI CAynn'nnrTG
Cuts at: 2338 2465 2338
Size: 127 4222
MspA1I CmG'CkG
Cuts at: 180 325 426 948 1460 1774 2134 3147 3392
Size: 145 101 522 512 314 360 1013 245
Cuts at: 3392 180
Size: 1137
Fragments arranged by size:
1137 1013 522 512 360 314 245 145
101
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NcoI C'CATG_G
Cuts at: 107 107
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NsiI A_TGCA'T
Cuts at: 939 939
Size: 4349
NspI r_CATG'y
Cuts at: 176 937 941 1116 1402 1902 2809 176
Size: 761 4 175 286 500 907 1716
Fragments arranged by size:
1716 907 761 500 286 175 4
PasI CC'CwG_GG
Cuts at: 147 348 736 906 1284 147
Size: 201 388 170 378 3212
Fragments arranged by size:
3212 388 378 201 170
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PflMI CCAn_nnn'nTGG
Cuts at: 153 153
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PpuMI rG'GwC_Cy
Cuts at: 846 859 1027 846
Size: 13 168 4168
Fragments arranged by size:
4168 168 13
PspOMI G'GGCC_C
Cuts at: 344 351 1321 344
Size: 7 970 3372
Fragments arranged by size:
3372 970 7
PspXI vC'TCGA_Gb
Cuts at: 719 719
Size: 4349
PstI C_TGCA'G
Cuts at: 122 1396 2081 122
Size: 1274 685 2390
Fragments arranged by size:
2390 1274 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 325 426 948 1774 2134 325
Size: 101 522 826 360 2540
Fragments arranged by size:
2540 826 522 360 101
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 437 746 1363 437
Size: 309 617 3423
Fragments arranged by size:
3423 617 309
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SanDI GG'GwC_CC
Cuts at: 859 859
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 814 1151 2372 2582 814
Size: 337 1221 210 2581
Fragments arranged by size:
2581 1221 337 210
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SfcI C'TryA_G
Cuts at: 33 118 220 1392 2077 3070 3261 33
Size: 85 102 1172 685 993 191 1121
Fragments arranged by size:
1172 1121 993 685 191 102 85
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 1371 1371
Size: 4349
SmlI C'TyrA_G
Cuts at: 719 1504 2419 2911 3173 719
Size: 785 915 492 262 1895
Fragments arranged by size:
1895 915 785 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 176 937 941 1116 1402 1902 176
Size: 761 4 175 286 500 2623
Fragments arranged by size:
2623 761 500 286 175 4
StyI C'CwwG_G
Cuts at: 107 239 575 854 1483 1837 107
Size: 132 336 279 629 354 2619
Fragments arranged by size:
2619 629 354 336 279 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 694 1932 2065 2524 2733 694
Size: 1238 133 459 209 2310
Fragments arranged by size:
2310 1238 459 209 133
TatI w'GTAC_w
Cuts at: 683 3864 683
Size: 3181 1168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 786 818 3866 3898 786
Size: 32 3048 32 1237
Fragments arranged by size:
3048 1237 32 32
Tth111I GACn'n_nGTC
Cuts at: 885 2146 885
Size: 1261 3088
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 582 892 1844 582
Size: 310 952 3087
Fragments arranged by size:
3087 952 310
XhoI C'TCGA_G
Cuts at: 719 719
Size: 4349
XmaI C'CCGG_G
Cuts at: 1369 1369
Size: 4349
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BmgBI BmtI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PfoI PmlI PshAI PsiI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI