Restriction map for
pF1KB7430
(Circular) (Six-base) MAPSORT of: pF1KB7430.seq Check: 9794 from: 1 to: 4601
pF1KB7430 4601 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1277 1277
Size: 4601
AccI GT'mk_AC
Cuts at: 863 1639 863
Size: 776 3825
Acc65I G'GTAC_C
Cuts at: 1617 1617
Size: 4601
AclI AA'CG_TT
Cuts at: 4441 4441
Size: 4601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2858 3605 3873 2426
Size: 432 747 268 3154
Fragments arranged by size:
3154 747 432 268
AfeI AGC'GCT
Cuts at: 432 432
Size: 4601
AflIII A'CryG_T
Cuts at: 1953 3057 4034 1953
Size: 1104 977 2520
Fragments arranged by size:
2520 1104 977
AgeI A'CCGG_T
Cuts at: 2989 2989
Size: 4601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1466 1466
Size: 4601
AlwNI CAG_nnn'CTG
Cuts at: 1906 3473 1906
Size: 1567 3034
ApaLI G'TGCA_C
Cuts at: 3371 3371
Size: 4601
ApoI r'AATT_y
Cuts at: 799 1435 1605 3954 4210 799
Size: 636 170 2349 256 1190
Fragments arranged by size:
2349 1190 636 256 170
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 1621 1621
Size: 4601
BamHI G'GATC_C
Cuts at: 1626 1626
Size: 4601
BanI G'GyrC_C
Cuts at: 1617 2031 2279 2314 1617
Size: 414 248 35 3904
Fragments arranged by size:
3904 414 248 35
BanII G_rGCy'C
Cuts at: 165 1305 1615 2645 165
Size: 1140 310 1030 2121
Fragments arranged by size:
2121 1140 1030 310
BbeI G_GCGC'C
Cuts at: 2035 2283 2035
Size: 248 4353
BbsI GAAGACnn'nnnn_
Cuts at: 1100 1100
Size: 4601
BbvCI CC'TCA_GC
Cuts at: 1588 1588
Size: 4601
BciVI GTATCCnnnnn_n'
Cuts at: 2499 3260 4601 2499
Size: 761 1341 2499
Fragments arranged by size:
2499 1341 761
BclI T'GATC_A
Cuts at: 2121 2121
Size: 4601
BglI GCCn_nnn'nGGC
Cuts at: 620 620
Size: 4601
BglII A'GATC_T
Cuts at: 2116 4153 2116
Size: 2037 2564
BlpI GC'TnA_GC
Cuts at: 1713 1713
Size: 4601
Bme1580I G_kGCm'C
Cuts at: 2226 2319 3375 2226
Size: 93 1056 3452
Fragments arranged by size:
3452 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1813 1894 2229 4373 1813
Size: 81 335 2144 2041
Fragments arranged by size:
2144 2041 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 549 2968 549
Size: 2419 2182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1372 1372
Size: 4601
Bpu10I CC'TnA_GC
Cuts at: 674 739 1588 674
Size: 65 849 3687
Fragments arranged by size:
3687 849 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1777 2656 3148 3446 1777
Size: 879 492 298 2932
Fragments arranged by size:
2932 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4305 4305
Size: 4601
BsaAI yAC'GTr
Cuts at: 1954 1954
Size: 4601
BsaBI GATnn'nnATC
Cuts at: 6 2115 4152 6
Size: 2109 2037 455
Fragments arranged by size:
2109 2037 455
BsaHI Gr'CG_yC
Cuts at: 2032 2280 2982 4478 2032
Size: 248 702 1496 2155
Fragments arranged by size:
2155 1496 702 248
BsaWI w'CCGG_w
Cuts at: 129 1790 2311 2989 3263 3410 3821 129
Size: 1661 521 678 274 147 411 909
Fragments arranged by size:
1661 909 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 264 294 264
Size: 30 4571
BseYI C'CCAG_C
Cuts at: 2026 2784 3361 3857 2026
Size: 758 577 496 2770
Fragments arranged by size:
2770 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 144 1245 2143 2189 3397 104
Size: 40 1101 898 46 1208 1308
Fragments arranged by size:
1308 1208 1101 898 46 40
BsiHKAI G_wGCw'C
Cuts at: 1615 2393 2583 3375 1615
Size: 778 190 792 2841
Fragments arranged by size:
2841 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 165 1305 1615 2226 2319 2393 2583 2645 3375
Size: 1140 310 611 93 74 190 62 730
Cuts at: 3375 165
Size: 1391
Fragments arranged by size:
1391 1140 730 611 310 190 93 74
62
BspEI T'CCGG_A
Cuts at: 129 3821 129
Size: 3692 909
BspHI T'CATG_A
Cuts at: 3777 3777
Size: 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 216 1116 1277 1651 1865 2167 2548 216
Size: 900 161 374 214 302 381 2269
Fragments arranged by size:
2269 900 381 374 302 214 161
BsrBI CCG'CTC
Cuts at: 50 2893 2947 4431 4596 50
Size: 2843 54 1484 165 55
Fragments arranged by size:
2843 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2513 2513
Size: 4601
BsrFI r'CCGG_y
Cuts at: 178 2599 2780 2989 178
Size: 2421 181 209 1790
Fragments arranged by size:
2421 1790 209 181
BssSI C'ACGA_G
Cuts at: 2872 3230 2872
Size: 358 4243
BstAPI GCAn_nnn'nTGC
Cuts at: 629 1978 629
Size: 1349 3252
BstBI TT'CG_AA
Cuts at: 2962 2962
Size: 4601
BstYI r'GATC_y
Cuts at: 1 1626 2093 2116 2451 2697 3709 3795 3807
Size: 1625 467 23 335 246 1012 86 12
Cuts at: 3807 4153 1
Size: 346 449
Fragments arranged by size:
1625 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2551 2732 2895 2551
Size: 181 163 4257
Fragments arranged by size:
4257 181 163
BtsI GCAGTG_nn'
Cuts at: 593 1844 593
Size: 1251 3350
ClaI AT'CG_AT
Cuts at: 1241 1241
Size: 4601
DraI TTT'AAA
Cuts at: 1600 1600
Size: 4601
DrdI GACnn_nn'nnGTC
Cuts at: 2307 3165 2307
Size: 858 3743
EaeI y'GGCC_r
Cuts at: 141 559 612 2186 2360 2751 2778 4123 141
Size: 418 53 1574 174 391 27 1345 619
Fragments arranged by size:
1574 1345 619 418 391 174 53 27
EagI C'GGCC_G
Cuts at: 141 2186 141
Size: 2045 2556
EarI CTCTTCn'nnn_
Cuts at: 401 540 632 840 1514 2624 2834 401
Size: 139 92 208 674 1110 210 2168
Fragments arranged by size:
2168 1110 674 210 208 139 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 173 2809 3119 3265 4411 173
Size: 2636 310 146 1146 363
Fragments arranged by size:
2636 1146 363 310 146
EcoICRI GAG'CTC
Cuts at: 1613 1613
Size: 4601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1372 2426 2858 3605 3873 1372
Size: 1054 432 747 268 2100
Fragments arranged by size:
2100 1054 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1034 1034
Size: 4601
EcoO109I rG'GnC_Cy
Cuts at: 1740 1740
Size: 4601
EcoRI G'AATT_C
Cuts at: 1605 1605
Size: 4601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 840 1231 2620 840
Size: 391 1389 2821
Fragments arranged by size:
2821 1389 391
FspI TGC'GCA
Cuts at: 2382 2382
Size: 4601
HaeII r_GCGC'y
Cuts at: 120 434 2035 2283 3305 4284 120
Size: 314 1601 248 1022 979 437
Fragments arranged by size:
1601 1022 979 437 314 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 2443 2473 2475 2505 178
Size: 32 2233 30 2 30 2274
Fragments arranged by size:
2274 2233 32 30 30 2
HincII GTy'rAC
Cuts at: 952 1576 1640 952
Size: 624 64 3913
Fragments arranged by size:
3913 624 64
HindIII A'AGCT_T
Cuts at: 1447 1447
Size: 4601
KasI G'GCGC_C
Cuts at: 2031 2279 2031
Size: 248 4353
KpnI G_GTAC'C
Cuts at: 1621 1621
Size: 4601
MfeI C'AATT_G
Cuts at: 701 701
Size: 4601
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3272 3456 3272
Size: 184 4417
MscI TGG'CCA
Cuts at: 614 2362 614
Size: 1748 2853
MslI CAynn'nnrTG
Cuts at: 198 1271 2590 2717 198
Size: 1073 1319 127 2082
Fragments arranged by size:
2082 1319 1073 127
MspA1I CmG'CkG
Cuts at: 140 1017 1712 2026 2386 3399 3644 140
Size: 877 695 314 360 1013 245 1097
Fragments arranged by size:
1097 1013 877 695 360 314 245
NaeI GCC'GGC
Cuts at: 180 2782 180
Size: 2602 1999
NarI GG'CG_CC
Cuts at: 2032 2280 2032
Size: 248 4353
NgoMIV G'CCGG_C
Cuts at: 178 2780 178
Size: 2602 1999
NspI r_CATG'y
Cuts at: 1312 1654 2154 3061 1312
Size: 342 500 907 2852
Fragments arranged by size:
2852 907 500 342
PciI A'CATG_T
Cuts at: 3057 3057
Size: 4601
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4569
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 953 985 953
Size: 32 4569
PstI C_TGCA'G
Cuts at: 1648 2333 1648
Size: 685 3916
PvuI CG_AT'CG
Cuts at: 104 1245 104
Size: 1141 3460
PvuII CAG'CTG
Cuts at: 1017 2026 2386 1017
Size: 1009 360 3232
Fragments arranged by size:
3232 1009 360
RsrII CG'GwC_CG
Cuts at: 2796 2796
Size: 4601
SacI G_AGCT'C
Cuts at: 1615 1615
Size: 4601
SalI G'TCGA_C
Cuts at: 1638 1638
Size: 4601
SapI GCTCTTCn'nnn_
Cuts at: 2624 2834 2624
Size: 210 4391
SbfI CC_TGCA'GG
Cuts at: 1648 1648
Size: 4601
ScaI AGT'ACT
Cuts at: 4118 4118
Size: 4601
SfcI C'TryA_G
Cuts at: 33 810 860 1644 2329 3322 3513 33
Size: 777 50 784 685 993 191 1121
Fragments arranged by size:
1121 993 784 777 685 191 50
SfoI GGC'GCC
Cuts at: 2033 2281 2033
Size: 248 4353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 1623 1623
Size: 4601
SmlI C'TyrA_G
Cuts at: 1756 2671 3163 3425 1756
Size: 915 492 262 2932
Fragments arranged by size:
2932 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
SspI AAT'ATT
Cuts at: 937 937
Size: 4601
StyI C'CwwG_G
Cuts at: 1072 1735 2089 1072
Size: 663 354 3584
Fragments arranged by size:
3584 663 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2184 2317 2776 2985 2184
Size: 133 459 209 3800
Fragments arranged by size:
3800 459 209 133
TatI w'GTAC_w
Cuts at: 484 4116 484
Size: 3632 969
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 225 257 4118 4150 225
Size: 32 3861 32 676
Fragments arranged by size:
3861 676 32 32
Tth111I GACn'n_nGTC
Cuts at: 2398 2398
Size: 4601
XbaI T'CTAG_A
Cuts at: 1632 1632
Size: 4601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 569 2096 569
Size: 1527 3074
XmaI C'CCGG_G
Cuts at: 1621 1621
Size: 4601
XmnI GAAnn'nnTTC
Cuts at: 553 1097 1214 553
Size: 544 117 3940
Fragments arranged by size:
3940 544 117
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI CspCI DraIII EcoRV FseI FspAI HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI