Restriction map for
pF1KB7431
(Circular) (Six-base) MAPSORT of: pF1KB7431.seq Check: 3657 from: 1 to: 4037
pF1KB7431 4037 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 1009 1009
Size: 4037
AccI GT'mk_AC
Cuts at: 1075 1075
Size: 4037
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 1010 1862 2294 3041 3309 210
Size: 800 852 432 747 268 938
Fragments arranged by size:
938 852 800 747 432 268
AflIII A'CryG_T
Cuts at: 1389 2493 3470 1389
Size: 1104 977 1956
Fragments arranged by size:
1956 1104 977
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 976 976
Size: 4037
AlwNI CAG_nnn'CTG
Cuts at: 427 1342 2909 427
Size: 915 1567 1555
Fragments arranged by size:
1567 1555 915
ApaI G_GGCC'C
Cuts at: 413 413
Size: 4037
ApaLI G'TGCA_C
Cuts at: 2807 2807
Size: 4037
ApoI r'AATT_y
Cuts at: 1041 3390 3646 1041
Size: 2349 256 1432
Fragments arranged by size:
2349 1432 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 1057 1057
Size: 4037
BamHI G'GATC_C
Cuts at: 1062 1062
Size: 4037
BanI G'GyrC_C
Cuts at: 138 217 553 1053 1467 1715 1750 138
Size: 79 336 500 414 248 35 2425
Fragments arranged by size:
2425 500 414 336 248 79 35
BanII G_rGCy'C
Cuts at: 124 413 1051 2081 124
Size: 289 638 1030 2080
Fragments arranged by size:
2080 1030 638 289
BbeI G_GCGC'C
Cuts at: 557 1471 1719 557
Size: 914 248 2875
Fragments arranged by size:
2875 914 248
BbsI GAAGACnn'nnnn_
Cuts at: 647 647
Size: 4037
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4003
BciVI GTATCCnnnnn_n'
Cuts at: 830 1935 2696 4037 830
Size: 1105 761 1341 830
Fragments arranged by size:
1341 1105 830 761
BclI T'GATC_A
Cuts at: 623 1557 623
Size: 934 3103
BglI GCCn_nnn'nGGC
Cuts at: 119 147 382 490 119
Size: 28 235 108 3666
Fragments arranged by size:
3666 235 108 28
BglII A'GATC_T
Cuts at: 1552 3589 1552
Size: 2037 2000
BlpI GC'TnA_GC
Cuts at: 649 1149 649
Size: 500 3537
Bme1580I G_kGCm'C
Cuts at: 220 387 413 1662 1755 2811 220
Size: 167 26 1249 93 1056 1446
Fragments arranged by size:
1446 1249 1056 167 93 26
BmrI ACTGGGnnnn_n'
Cuts at: 1014 1249 1330 1665 3809 1014
Size: 235 81 335 2144 1242
Fragments arranged by size:
2144 1242 335 235 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 484 688 454
Size: 30 204 3803
Fragments arranged by size:
3803 204 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 2092 2584 2882 1213
Size: 879 492 298 2368
Fragments arranged by size:
2368 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3741 3741
Size: 4037
BsaAI yAC'GTr
Cuts at: 1390 1390
Size: 4037
BsaBI GATnn'nnATC
Cuts at: 6 1551 3588 6
Size: 1545 2037 455
Fragments arranged by size:
2037 1545 455
BsaHI Gr'CG_yC
Cuts at: 554 1006 1468 1716 2418 3914 554
Size: 452 462 248 702 1496 677
Fragments arranged by size:
1496 702 677 462 452 248
BsaWI w'CCGG_w
Cuts at: 822 1226 1747 2425 2699 2846 3257 822
Size: 404 521 678 274 147 411 1602
Fragments arranged by size:
1602 678 521 411 404 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 304 358 361 385 451 304
Size: 54 3 24 66 3890
Fragments arranged by size:
3890 66 54 24 3
BseYI C'CCAG_C
Cuts at: 377 1462 2220 2797 3293 377
Size: 1085 758 577 496 1121
Fragments arranged by size:
1121 1085 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 413 262
Size: 151 3886
BsiEI CG_ry'CG
Cuts at: 104 257 1579 1625 2833 104
Size: 153 1322 46 1208 1308
Fragments arranged by size:
1322 1308 1208 153 46
BsiHKAI G_wGCw'C
Cuts at: 1051 1829 2019 2811 1051
Size: 778 190 792 2277
Fragments arranged by size:
2277 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 997 997
Size: 4037
Bsp1286I G_dGCh'C
Cuts at: 124 220 387 413 1051 1662 1755 1829 2019
Size: 96 167 26 638 611 93 74 190
Cuts at: 2019 2081 2811 124
Size: 62 730 1350
Fragments arranged by size:
1350 730 638 611 190 167 96 93
74 62 26
BspEI T'CCGG_A
Cuts at: 822 3257 822
Size: 2435 1602
BspHI T'CATG_A
Cuts at: 3213 3213
Size: 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1087 1301 1603 1984 1087
Size: 214 302 381 3140
Fragments arranged by size:
3140 381 302 214
BsrBI CCG'CTC
Cuts at: 50 363 522 2329 2383 3867 4032 50
Size: 313 159 1807 54 1484 165 55
Fragments arranged by size:
1807 1484 313 165 159 55 54
BsrDI GCAATG_nn'
Cuts at: 1949 1949
Size: 4037
BsrFI r'CCGG_y
Cuts at: 468 2035 2216 2425 468
Size: 1567 181 209 2080
Fragments arranged by size:
2080 1567 209 181
BssSI C'ACGA_G
Cuts at: 355 2308 2666 355
Size: 1953 358 1726
Fragments arranged by size:
1953 1726 358
BstAPI GCAn_nnn'nTGC
Cuts at: 396 1414 396
Size: 1018 3019
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstXI CCAn_nnnn'nTGG
Cuts at: 815 815
Size: 4037
BstYI r'GATC_y
Cuts at: 1 910 929 1062 1529 1552 1887 2133 3145
Size: 909 19 133 467 23 335 246 1012
Cuts at: 3145 3231 3243 3589 1
Size: 86 12 346 449
Fragments arranged by size:
1012 909 467 449 346 335 246 133
86 23 19 12
Bsu36I CC'TnA_GG
Cuts at: 116 116
Size: 4037
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1987 2168 2331 1987
Size: 181 163 3693
Fragments arranged by size:
3693 181 163
BtsI GCAGTG_nn'
Cuts at: 1280 1280
Size: 4037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 792 827 792
Size: 35 4002
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 423 1743 2601 423
Size: 1320 858 1859
Fragments arranged by size:
1859 1320 858
EaeI y'GGCC_r
Cuts at: 254 446 1622 1796 2187 2214 3559 254
Size: 192 1176 174 391 27 1345 732
Fragments arranged by size:
1345 1176 732 391 192 174 27
EagI C'GGCC_G
Cuts at: 254 1622 254
Size: 1368 2669
EarI CTCTTCn'nnn_
Cuts at: 674 986 2060 2270 674
Size: 312 1074 210 2441
Fragments arranged by size:
2441 1074 312 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 337 355 2245 2555 2701 3847 337
Size: 18 1890 310 146 1146 527
Fragments arranged by size:
1890 1146 527 310 146 18
EcoICRI GAG'CTC
Cuts at: 1049 1049
Size: 4037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 454 484 688 1010 1862 2294 3041 3309
Size: 244 30 204 322 852 432 747 268
Cuts at: 3309 210
Size: 938
Fragments arranged by size:
938 852 747 432 322 268 244 204
30
EcoNI CCTnn'n_nnAGG
Cuts at: 937 937
Size: 4037
EcoO109I rG'GnC_Cy
Cuts at: 351 942 1176 351
Size: 591 234 3212
Fragments arranged by size:
3212 591 234
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 4037
FspI TGC'GCA
Cuts at: 1818 1818
Size: 4037
HaeII r_GCGC'y
Cuts at: 557 1471 1719 2741 3720 557
Size: 914 248 1022 979 874
Fragments arranged by size:
1022 979 914 874 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 542 574 1879 1909 1911 1941 542
Size: 32 1305 30 2 30 2638
Fragments arranged by size:
2638 1305 32 30 30 2
HincII GTy'rAC
Cuts at: 1076 1076
Size: 4037
KasI G'GCGC_C
Cuts at: 553 1467 1715 553
Size: 914 248 2875
Fragments arranged by size:
2875 914 248
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MfeI C'AATT_G
Cuts at: 809 809
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 287 2708 2892 287
Size: 2421 184 1432
Fragments arranged by size:
2421 1432 184
MscI TGG'CCA
Cuts at: 1798 1798
Size: 4037
MslI CAynn'nnrTG
Cuts at: 2026 2153 2026
Size: 127 3910
MspA1I CmG'CkG
Cuts at: 381 507 1148 1462 1822 2835 3080 381
Size: 126 641 314 360 1013 245 1338
Fragments arranged by size:
1338 1013 641 360 314 245 126
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 4037
NarI GG'CG_CC
Cuts at: 554 1468 1716 554
Size: 914 248 2875
Fragments arranged by size:
2875 914 248
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 4037
NspI r_CATG'y
Cuts at: 648 1090 1590 2497 648
Size: 442 500 907 2188
Fragments arranged by size:
2188 907 500 442
PasI CC'CwG_GG
Cuts at: 406 406
Size: 4037
PciI A'CATG_T
Cuts at: 2493 2493
Size: 4037
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PpuMI rG'GwC_Cy
Cuts at: 942 942
Size: 4037
PspOMI G'GGCC_C
Cuts at: 409 409
Size: 4037
PstI C_TGCA'G
Cuts at: 1084 1769 1084
Size: 685 3352
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 1462 1822 1462
Size: 360 3677
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 1051 1051
Size: 4037
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SapI GCTCTTCn'nnn_
Cuts at: 2060 2270 2060
Size: 210 3827
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SfcI C'TryA_G
Cuts at: 33 1080 1765 2758 2949 33
Size: 1047 685 993 191 1121
Fragments arranged by size:
1121 1047 993 685 191
SfoI GGC'GCC
Cuts at: 555 1469 1717 555
Size: 914 248 2875
Fragments arranged by size:
2875 914 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 1192 2107 2599 2861 1192
Size: 915 492 262 2368
Fragments arranged by size:
2368 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1090 1590 1090
Size: 500 3537
StuI AGG'CCT
Cuts at: 190 493 841 190
Size: 303 348 3386
Fragments arranged by size:
3386 348 303
StyI C'CwwG_G
Cuts at: 191 209 758 1171 1525 191
Size: 18 549 413 354 2703
Fragments arranged by size:
2703 549 413 354 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1620 1753 2212 2421 1620
Size: 133 459 209 3236
Fragments arranged by size:
3236 459 209 133
TatI w'GTAC_w
Cuts at: 3552 3552
Size: 4037
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4005
Tth111I GACn'n_nGTC
Cuts at: 1834 1834
Size: 4037
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 571 765 1532 571
Size: 194 767 3076
Fragments arranged by size:
3076 767 194
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
ZraI GAC'GTC
Cuts at: 1007 1007
Size: 4037
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I BtgI ClaI DraIII
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI