Restriction map for
pF1KB7433
(Circular) (Six-base) MAPSORT of: pF1KB7433.seq Check: 5731 from: 1 to: 4070
pF1KB7433 4070 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1108 1108
Size: 4070
Acc65I G'GTAC_C
Cuts at: 1086 1086
Size: 4070
AclI AA'CG_TT
Cuts at: 3910 3910
Size: 4070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 821 1030 1895 2327 3074 3342 821
Size: 209 865 432 747 268 1549
Fragments arranged by size:
1549 865 747 432 268 209
AflIII A'CryG_T
Cuts at: 761 1422 2526 3503 761
Size: 661 1104 977 1328
Fragments arranged by size:
1328 1104 977 661
AgeI A'CCGG_T
Cuts at: 2458 2458
Size: 4070
AlwNI CAG_nnn'CTG
Cuts at: 1375 2942 1375
Size: 1567 2503
ApaLI G'TGCA_C
Cuts at: 2840 2840
Size: 4070
ApoI r'AATT_y
Cuts at: 357 1074 3423 3679 357
Size: 717 2349 256 748
Fragments arranged by size:
2349 748 717 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4070
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
AvaI C'yCGr_G
Cuts at: 1090 1090
Size: 4070
BamHI G'GATC_C
Cuts at: 1095 1095
Size: 4070
BanI G'GyrC_C
Cuts at: 865 1086 1500 1748 1783 865
Size: 221 414 248 35 3152
Fragments arranged by size:
3152 414 248 221 35
BanII G_rGCy'C
Cuts at: 796 1084 2114 796
Size: 288 1030 2752
Fragments arranged by size:
2752 1030 288
BbeI G_GCGC'C
Cuts at: 1504 1752 1504
Size: 248 3822
BbsI GAAGACnn'nnnn_
Cuts at: 813 927 813
Size: 114 3956
BciVI GTATCCnnnnn_n'
Cuts at: 255 1968 2729 4070 255
Size: 1713 761 1341 255
Fragments arranged by size:
1713 1341 761 255
BclI T'GATC_A
Cuts at: 1590 1590
Size: 4070
BfrBI ATG'CAT
Cuts at: 394 394
Size: 4070
BglII A'GATC_T
Cuts at: 1585 3622 1585
Size: 2037 2033
BlpI GC'TnA_GC
Cuts at: 795 1182 795
Size: 387 3683
Bme1580I G_kGCm'C
Cuts at: 1695 1788 2844 1695
Size: 93 1056 2921
Fragments arranged by size:
2921 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 418 1282 1363 1698 3842 418
Size: 864 81 335 2144 646
Fragments arranged by size:
2144 864 646 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 503
Size: 4070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 882 1246 2125 2617 2915 882
Size: 364 879 492 298 2037
Fragments arranged by size:
2037 879 492 364 298
BsaI GGTCTCn'nnnn_
Cuts at: 3774 3774
Size: 4070
BsaAI yAC'GTr
Cuts at: 1423 1423
Size: 4070
BsaBI GATnn'nnATC
Cuts at: 6 1584 3621 6
Size: 1578 2037 455
Fragments arranged by size:
2037 1578 455
BsaHI Gr'CG_yC
Cuts at: 1501 1749 2451 3947 1501
Size: 248 702 1496 1624
Fragments arranged by size:
1624 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1259 1780 2458 2732 2879 3290 1259
Size: 521 678 274 147 411 2039
Fragments arranged by size:
2039 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 649 649
Size: 4070
BseYI C'CCAG_C
Cuts at: 1495 2253 2830 3326 1495
Size: 758 577 496 2239
Fragments arranged by size:
2239 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1612 1658 2866 104
Size: 1508 46 1208 1308
Fragments arranged by size:
1508 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 796 1084 1862 2052 2844 796
Size: 288 778 190 792 2022
Fragments arranged by size:
2022 792 778 288 190
BsmI GAATG_Cn'
Cuts at: 530 530
Size: 4070
Bsp1286I G_dGCh'C
Cuts at: 796 1084 1695 1788 1862 2052 2114 2844 796
Size: 288 611 93 74 190 62 730 2022
Fragments arranged by size:
2022 730 611 288 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3290 3290
Size: 4070
BspHI T'CATG_A
Cuts at: 3246 3246
Size: 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1120 1334 1636 2017 1120
Size: 214 302 381 3173
Fragments arranged by size:
3173 381 302 214
BsrBI CCG'CTC
Cuts at: 50 259 2362 2416 3900 4065 50
Size: 209 2103 54 1484 165 55
Fragments arranged by size:
2103 1484 209 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1982 1982
Size: 4070
BsrFI r'CCGG_y
Cuts at: 2068 2249 2458 2068
Size: 181 209 3680
Fragments arranged by size:
3680 209 181
BsrGI T'GTAC_A
Cuts at: 365 365
Size: 4070
BssSI C'ACGA_G
Cuts at: 2341 2699 2341
Size: 358 3712
BstAPI GCAn_nnn'nTGC
Cuts at: 1447 1447
Size: 4070
BstBI TT'CG_AA
Cuts at: 2431 2431
Size: 4070
BstYI r'GATC_y
Cuts at: 1 1095 1562 1585 1920 2166 3178 3264 3276
Size: 1094 467 23 335 246 1012 86 12
Cuts at: 3276 3622 1
Size: 346 449
Fragments arranged by size:
1094 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 661 784 661
Size: 123 3947
BtgI C'CryG_G
Cuts at: 107 458 107
Size: 351 3719
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2020 2201 2364 2020
Size: 181 163 3726
Fragments arranged by size:
3726 181 163
BtsI GCAGTG_nn'
Cuts at: 1313 1313
Size: 4070
DraI TTT'AAA
Cuts at: 1069 1069
Size: 4070
DrdI GACnn_nn'nnGTC
Cuts at: 1776 2634 1776
Size: 858 3212
EaeI y'GGCC_r
Cuts at: 479 1655 1829 2220 2247 3592 479
Size: 1176 174 391 27 1345 957
Fragments arranged by size:
1345 1176 957 391 174 27
EagI C'GGCC_G
Cuts at: 1655 1655
Size: 4070
EarI CTCTTCn'nnn_
Cuts at: 220 333 2093 2303 220
Size: 113 1760 210 1987
Fragments arranged by size:
1987 1760 210 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 589 2278 2588 2734 3880 589
Size: 1689 310 146 1146 779
Fragments arranged by size:
1689 1146 779 310 146
EcoICRI GAG'CTC
Cuts at: 794 1082 794
Size: 288 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 821 1030 1895 2327 3074 3342 503
Size: 318 209 865 432 747 268 1231
Fragments arranged by size:
1231 865 747 432 318 268 209
EcoO109I rG'GnC_Cy
Cuts at: 1209 1209
Size: 4070
EcoRI G'AATT_C
Cuts at: 1074 1074
Size: 4070
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 4070
FspI TGC'GCA
Cuts at: 1851 1851
Size: 4070
HaeII r_GCGC'y
Cuts at: 1504 1752 2774 3753 1504
Size: 248 1022 979 1821
Fragments arranged by size:
1821 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 963 995 1912 1942 1944 1974 963
Size: 32 917 30 2 30 3059
Fragments arranged by size:
3059 917 32 30 30 2
HincII GTy'rAC
Cuts at: 630 1109 630
Size: 479 3591
KasI G'GCGC_C
Cuts at: 1500 1748 1500
Size: 248 3822
KpnI G_GTAC'C
Cuts at: 1090 1090
Size: 4070
MfeI C'AATT_G
Cuts at: 120 120
Size: 4070
MluI A'CGCG_T
Cuts at: 3503 3503
Size: 4070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 644 2741 2925 644
Size: 2097 184 1789
Fragments arranged by size:
2097 1789 184
MscI TGG'CCA
Cuts at: 481 1831 481
Size: 1350 2720
MslI CAynn'nnrTG
Cuts at: 280 427 2059 2186 280
Size: 147 1632 127 2164
Fragments arranged by size:
2164 1632 147 127
MspA1I CmG'CkG
Cuts at: 799 1181 1495 1855 2868 3113 799
Size: 382 314 360 1013 245 1756
Fragments arranged by size:
1756 1013 382 360 314 245
NaeI GCC'GGC
Cuts at: 2251 2251
Size: 4070
NarI GG'CG_CC
Cuts at: 1501 1749 1501
Size: 248 3822
NcoI C'CATG_G
Cuts at: 107 458 107
Size: 351 3719
NgoMIV G'CCGG_C
Cuts at: 2249 2249
Size: 4070
NsiI A_TGCA'T
Cuts at: 396 396
Size: 4070
NspI r_CATG'y
Cuts at: 398 654 765 843 1123 1623 2530 398
Size: 256 111 78 280 500 907 1938
Fragments arranged by size:
1938 907 500 280 256 111 78
PciI A'CATG_T
Cuts at: 761 2526 761
Size: 1765 2305
PfoI T'CCnGG_A
Cuts at: 519 519
Size: 4070
PmeI GTTT'AAAC
Cuts at: 1069 1069
Size: 4070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 4038
PstI C_TGCA'G
Cuts at: 1117 1802 1117
Size: 685 3385
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4070
PvuII CAG'CTG
Cuts at: 799 1495 1855 799
Size: 696 360 3014
Fragments arranged by size:
3014 696 360
RsrII CG'GwC_CG
Cuts at: 2265 2265
Size: 4070
SacI G_AGCT'C
Cuts at: 796 1084 796
Size: 288 3782
SalI G'TCGA_C
Cuts at: 1107 1107
Size: 4070
SapI GCTCTTCn'nnn_
Cuts at: 2093 2303 2093
Size: 210 3860
SbfI CC_TGCA'GG
Cuts at: 1117 1117
Size: 4070
ScaI AGT'ACT
Cuts at: 3587 3587
Size: 4070
SfcI C'TryA_G
Cuts at: 33 558 828 1113 1798 2791 2982 33
Size: 525 270 285 685 993 191 1121
Fragments arranged by size:
1121 993 685 525 285 270 191
SfoI GGC'GCC
Cuts at: 1502 1750 1502
Size: 248 3822
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
SmaI CCC'GGG
Cuts at: 1092 1092
Size: 4070
SmlI C'TyrA_G
Cuts at: 897 1225 2140 2632 2894 897
Size: 328 915 492 262 2073
Fragments arranged by size:
2073 915 492 328 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4070
SphI G_CATG'C
Cuts at: 1123 1623 1123
Size: 500 3570
StyI C'CwwG_G
Cuts at: 107 458 1204 1558 107
Size: 351 746 354 2619
Fragments arranged by size:
2619 746 354 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 142 357 485 1653 1786 2245 2454 142
Size: 215 128 1168 133 459 209 1758
Fragments arranged by size:
1758 1168 459 215 209 133 128
TatI w'GTAC_w
Cuts at: 284 365 881 3585 284
Size: 81 516 2704 769
Fragments arranged by size:
2704 769 516 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4038
Tth111I GACn'n_nGTC
Cuts at: 1867 1867
Size: 4070
XbaI T'CTAG_A
Cuts at: 1101 1101
Size: 4070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1565 1565
Size: 4070
XmaI C'CCGG_G
Cuts at: 1090 1090
Size: 4070
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI