Restriction map for
pF1KB7435
(Circular) (Six-base) MAPSORT of: pF1KB7435.seq Check: 2222 from: 1 to: 3533
pF1KB7435 3533 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 571 571
Size: 3533
Acc65I G'GTAC_C
Cuts at: 549 549
Size: 3533
AclI AA'CG_TT
Cuts at: 3373 3373
Size: 3533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1358 1790 2537 2805 229
Size: 1129 432 747 268 957
Fragments arranged by size:
1129 957 747 432 268
AflIII A'CryG_T
Cuts at: 337 885 1989 2966 337
Size: 548 1104 977 904
Fragments arranged by size:
1104 977 904 548
AgeI A'CCGG_T
Cuts at: 1921 1921
Size: 3533
AlwNI CAG_nnn'CTG
Cuts at: 838 2405 838
Size: 1567 1966
ApaLI G'TGCA_C
Cuts at: 2303 2303
Size: 3533
ApoI r'AATT_y
Cuts at: 182 537 2886 3142 182
Size: 355 2349 256 573
Fragments arranged by size:
2349 573 355 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3533
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
AvaI C'yCGr_G
Cuts at: 553 553
Size: 3533
BamHI G'GATC_C
Cuts at: 558 558
Size: 3533
BanI G'GyrC_C
Cuts at: 549 963 1211 1246 549
Size: 414 248 35 2836
Fragments arranged by size:
2836 414 248 35
BanII G_rGCy'C
Cuts at: 143 547 1577 143
Size: 404 1030 2099
Fragments arranged by size:
2099 1030 404
BbeI G_GCGC'C
Cuts at: 967 1215 967
Size: 248 3285
BciVI GTATCCnnnnn_n'
Cuts at: 1431 2192 3533 1431
Size: 761 1341 1431
Fragments arranged by size:
1431 1341 761
BclI T'GATC_A
Cuts at: 1053 1053
Size: 3533
BglI GCCn_nnn'nGGC
Cuts at: 238 238
Size: 3533
BglII A'GATC_T
Cuts at: 1048 3085 1048
Size: 2037 1496
BlpI GC'TnA_GC
Cuts at: 645 645
Size: 3533
Bme1580I G_kGCm'C
Cuts at: 1158 1251 2307 1158
Size: 93 1056 2384
Fragments arranged by size:
2384 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 281 745 826 1161 3305 281
Size: 464 81 335 2144 509
Fragments arranged by size:
2144 509 464 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3533
Bpu10I CC'TnA_GC
Cuts at: 145 145
Size: 3533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1588 2080 2378 709
Size: 879 492 298 1864
Fragments arranged by size:
1864 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3237 3237
Size: 3533
BsaAI yAC'GTr
Cuts at: 886 886
Size: 3533
BsaBI GATnn'nnATC
Cuts at: 6 194 1047 3084 6
Size: 188 853 2037 455
Fragments arranged by size:
2037 853 455 188
BsaHI Gr'CG_yC
Cuts at: 964 1212 1914 3410 964
Size: 248 702 1496 1087
Fragments arranged by size:
1496 1087 702 248
BsaWI w'CCGG_w
Cuts at: 722 1243 1921 2195 2342 2753 722
Size: 521 678 274 147 411 1502
Fragments arranged by size:
1502 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 151
Size: 3533
BseYI C'CCAG_C
Cuts at: 958 1716 2293 2789 958
Size: 758 577 496 1702
Fragments arranged by size:
1702 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 187
Size: 3533
BsiEI CG_ry'CG
Cuts at: 104 1075 1121 2329 104
Size: 971 46 1208 1308
Fragments arranged by size:
1308 1208 971 46
BsiHKAI G_wGCw'C
Cuts at: 143 547 1325 1515 2307 143
Size: 404 778 190 792 1369
Fragments arranged by size:
1369 792 778 404 190
BsmI GAATG_Cn'
Cuts at: 379 379
Size: 3533
Bsp1286I G_dGCh'C
Cuts at: 143 547 1158 1251 1325 1515 1577 2307 143
Size: 404 611 93 74 190 62 730 1369
Fragments arranged by size:
1369 730 611 404 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2753 2753
Size: 3533
BspHI T'CATG_A
Cuts at: 2709 2709
Size: 3533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 583 797 1099 1480 583
Size: 214 302 381 2636
Fragments arranged by size:
2636 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1825 1879 3363 3528 50
Size: 1775 54 1484 165 55
Fragments arranged by size:
1775 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1445 1445
Size: 3533
BsrFI r'CCGG_y
Cuts at: 123 1531 1712 1921 123
Size: 1408 181 209 1735
Fragments arranged by size:
1735 1408 209 181
BssHII G'CGCG_C
Cuts at: 176 176
Size: 3533
BssSI C'ACGA_G
Cuts at: 1804 2162 1804
Size: 358 3175
BstAPI GCAn_nnn'nTGC
Cuts at: 413 910 413
Size: 497 3036
BstBI TT'CG_AA
Cuts at: 1894 1894
Size: 3533
BstXI CCAn_nnnn'nTGG
Cuts at: 313 313
Size: 3533
BstYI r'GATC_y
Cuts at: 1 558 1025 1048 1383 1629 2641 2727 2739
Size: 557 467 23 335 246 1012 86 12
Cuts at: 2739 3085 1
Size: 346 449
Fragments arranged by size:
1012 557 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1483 1664 1827 1483
Size: 181 163 3189
Fragments arranged by size:
3189 181 163
BtsI GCAGTG_nn'
Cuts at: 776 776
Size: 3533
DraI TTT'AAA
Cuts at: 532 532
Size: 3533
DraIII CAC_nnn'GTG
Cuts at: 289 289
Size: 3533
DrdI GACnn_nn'nnGTC
Cuts at: 1239 2097 1239
Size: 858 2675
EaeI y'GGCC_r
Cuts at: 110 1118 1292 1683 1710 3055 110
Size: 1008 174 391 27 1345 588
Fragments arranged by size:
1345 1008 588 391 174 27
EagI C'GGCC_G
Cuts at: 1118 1118
Size: 3533
EarI CTCTTCn'nnn_
Cuts at: 391 1556 1766 391
Size: 1165 210 2158
Fragments arranged by size:
2158 1165 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1741 2051 2197 3343 1741
Size: 310 146 1146 1931
Fragments arranged by size:
1931 1146 310 146
EcoICRI GAG'CTC
Cuts at: 141 545 141
Size: 404 3129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1358 1790 2537 2805 229
Size: 1129 432 747 268 957
Fragments arranged by size:
1129 957 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 442 442
Size: 3533
EcoO109I rG'GnC_Cy
Cuts at: 309 455 672 309
Size: 146 217 3170
Fragments arranged by size:
3170 217 146
EcoRI G'AATT_C
Cuts at: 182 537 182
Size: 355 3178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 234 441 1552 234
Size: 207 1111 2215
Fragments arranged by size:
2215 1111 207
FspI TGC'GCA
Cuts at: 1314 1314
Size: 3533
HaeII r_GCGC'y
Cuts at: 967 1215 2237 3216 967
Size: 248 1022 979 1284
Fragments arranged by size:
1284 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 251 279 283 444 476 1375 1405 1407
Size: 4 28 4 161 32 899 30 2
Cuts at: 1407 1437 247
Size: 30 2343
Fragments arranged by size:
2343 899 161 32 30 30 28 4
4 2
HincII GTy'rAC
Cuts at: 491 572 491
Size: 81 3452
KasI G'GCGC_C
Cuts at: 963 1211 963
Size: 248 3285
KpnI G_GTAC'C
Cuts at: 553 553
Size: 3533
MluI A'CGCG_T
Cuts at: 2966 2966
Size: 3533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2388 2204
Size: 184 3349
MscI TGG'CCA
Cuts at: 1294 1294
Size: 3533
MslI CAynn'nnrTG
Cuts at: 336 1522 1649 336
Size: 1186 127 2220
Fragments arranged by size:
2220 1186 127
MspA1I CmG'CkG
Cuts at: 644 958 1318 2331 2576 644
Size: 314 360 1013 245 1601
Fragments arranged by size:
1601 1013 360 314 245
NaeI GCC'GGC
Cuts at: 125 1714 125
Size: 1589 1944
NarI GG'CG_CC
Cuts at: 964 1212 964
Size: 248 3285
NcoI C'CATG_G
Cuts at: 107 107
Size: 3533
NgoMIV G'CCGG_C
Cuts at: 123 1712 123
Size: 1589 1944
NspI r_CATG'y
Cuts at: 341 586 1086 1993 341
Size: 245 500 907 1881
Fragments arranged by size:
1881 907 500 245
PciI A'CATG_T
Cuts at: 337 1989 337
Size: 1652 1881
PflMI CCAn_nnn'nTGG
Cuts at: 243 243
Size: 3533
PmeI GTTT'AAAC
Cuts at: 532 532
Size: 3533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2672 2704 2672
Size: 32 3501
PpuMI rG'GwC_Cy
Cuts at: 309 455 309
Size: 146 3387
PstI C_TGCA'G
Cuts at: 580 1265 580
Size: 685 2848
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3533
PvuII CAG'CTG
Cuts at: 958 1318 958
Size: 360 3173
RsrII CG'GwC_CG
Cuts at: 1728 1728
Size: 3533
SacI G_AGCT'C
Cuts at: 143 547 143
Size: 404 3129
SalI G'TCGA_C
Cuts at: 570 570
Size: 3533
SapI GCTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
SbfI CC_TGCA'GG
Cuts at: 580 580
Size: 3533
ScaI AGT'ACT
Cuts at: 3050 3050
Size: 3533
SexAI A'CCwGG_T
Cuts at: 305 305
Size: 3533
SfcI C'TryA_G
Cuts at: 33 576 1261 2254 2445 33
Size: 543 685 993 191 1121
Fragments arranged by size:
1121 993 685 543 191
SfoI GGC'GCC
Cuts at: 965 1213 965
Size: 248 3285
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
SmaI CCC'GGG
Cuts at: 555 555
Size: 3533
SmlI C'TyrA_G
Cuts at: 688 1603 2095 2357 688
Size: 915 492 262 1864
Fragments arranged by size:
1864 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3533
SphI G_CATG'C
Cuts at: 586 1086 586
Size: 500 3033
StuI AGG'CCT
Cuts at: 241 241
Size: 3533
StyI C'CwwG_G
Cuts at: 107 242 312 667 1021 107
Size: 135 70 355 354 2619
Fragments arranged by size:
2619 355 354 135 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 250 300 1116 1249 1708 1917 250
Size: 50 816 133 459 209 1866
Fragments arranged by size:
1866 816 459 209 133 50
TatI w'GTAC_w
Cuts at: 3048 3048
Size: 3533
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3501
Tth111I GACn'n_nGTC
Cuts at: 1330 1330
Size: 3533
XbaI T'CTAG_A
Cuts at: 564 564
Size: 3533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 240 1028 240
Size: 788 2745
XmaI C'CCGG_G
Cuts at: 553 553
Size: 3533
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BpmI BsaXI BsiWI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI