(Circular) (Six-base) MAPSORT of: pF1KB7435.seq Check: 2222 from: 1 to: 3533 pF1KB7435 3533 bp With 182 enzymes: SgfI * June 20, 2008 10:27 .. AccI GT'mk_AC Cuts at: 571 571 Size: 3533 Acc65I G'GTAC_C Cuts at: 549 549 Size: 3533 AclI AA'CG_TT Cuts at: 3373 3373 Size: 3533 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 229 1358 1790 2537 2805 229 Size: 1129 432 747 268 957 Fragments arranged by size: 1129 957 747 432 268 AflIII A'CryG_T Cuts at: 337 885 1989 2966 337 Size: 548 1104 977 904 Fragments arranged by size: 1104 977 904 548 AgeI A'CCGG_T Cuts at: 1921 1921 Size: 3533 AlwNI CAG_nnn'CTG Cuts at: 838 2405 838 Size: 1567 1966 ApaLI G'TGCA_C Cuts at: 2303 2303 Size: 3533 ApoI r'AATT_y Cuts at: 182 537 2886 3142 182 Size: 355 2349 256 573 Fragments arranged by size: 2349 573 355 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3533 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3533 AvaI C'yCGr_G Cuts at: 553 553 Size: 3533 BamHI G'GATC_C Cuts at: 558 558 Size: 3533 BanI G'GyrC_C Cuts at: 549 963 1211 1246 549 Size: 414 248 35 2836 Fragments arranged by size: 2836 414 248 35 BanII G_rGCy'C Cuts at: 143 547 1577 143 Size: 404 1030 2099 Fragments arranged by size: 2099 1030 404 BbeI G_GCGC'C Cuts at: 967 1215 967 Size: 248 3285 BciVI GTATCCnnnnn_n' Cuts at: 1431 2192 3533 1431 Size: 761 1341 1431 Fragments arranged by size: 1431 1341 761 BclI T'GATC_A Cuts at: 1053 1053 Size: 3533 BglI GCCn_nnn'nGGC Cuts at: 238 238 Size: 3533 BglII A'GATC_T Cuts at: 1048 3085 1048 Size: 2037 1496 BlpI GC'TnA_GC Cuts at: 645 645 Size: 3533 Bme1580I G_kGCm'C Cuts at: 1158 1251 2307 1158 Size: 93 1056 2384 Fragments arranged by size: 2384 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 281 745 826 1161 3305 281 Size: 464 81 335 2144 509 Fragments arranged by size: 2144 509 464 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1900 1900 Size: 3533 Bpu10I CC'TnA_GC Cuts at: 145 145 Size: 3533 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 709 1588 2080 2378 709 Size: 879 492 298 1864 Fragments arranged by size: 1864 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3237 3237 Size: 3533 BsaAI yAC'GTr Cuts at: 886 886 Size: 3533 BsaBI GATnn'nnATC Cuts at: 6 194 1047 3084 6 Size: 188 853 2037 455 Fragments arranged by size: 2037 853 455 188 BsaHI Gr'CG_yC Cuts at: 964 1212 1914 3410 964 Size: 248 702 1496 1087 Fragments arranged by size: 1496 1087 702 248 BsaWI w'CCGG_w Cuts at: 722 1243 1921 2195 2342 2753 722 Size: 521 678 274 147 411 1502 Fragments arranged by size: 1502 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 151 151 Size: 3533 BseYI C'CCAG_C Cuts at: 958 1716 2293 2789 958 Size: 758 577 496 1702 Fragments arranged by size: 1702 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 187 187 Size: 3533 BsiEI CG_ry'CG Cuts at: 104 1075 1121 2329 104 Size: 971 46 1208 1308 Fragments arranged by size: 1308 1208 971 46 BsiHKAI G_wGCw'C Cuts at: 143 547 1325 1515 2307 143 Size: 404 778 190 792 1369 Fragments arranged by size: 1369 792 778 404 190 BsmI GAATG_Cn' Cuts at: 379 379 Size: 3533 Bsp1286I G_dGCh'C Cuts at: 143 547 1158 1251 1325 1515 1577 2307 143 Size: 404 611 93 74 190 62 730 1369 Fragments arranged by size: 1369 730 611 404 190 93 74 62 BspEI T'CCGG_A Cuts at: 2753 2753 Size: 3533 BspHI T'CATG_A Cuts at: 2709 2709 Size: 3533 BspMI ACCTGCnnnn'nnnn_ Cuts at: 583 797 1099 1480 583 Size: 214 302 381 2636 Fragments arranged by size: 2636 381 302 214 BsrBI CCG'CTC Cuts at: 50 1825 1879 3363 3528 50 Size: 1775 54 1484 165 55 Fragments arranged by size: 1775 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1445 1445 Size: 3533 BsrFI r'CCGG_y Cuts at: 123 1531 1712 1921 123 Size: 1408 181 209 1735 Fragments arranged by size: 1735 1408 209 181 BssHII G'CGCG_C Cuts at: 176 176 Size: 3533 BssSI C'ACGA_G Cuts at: 1804 2162 1804 Size: 358 3175 BstAPI GCAn_nnn'nTGC Cuts at: 413 910 413 Size: 497 3036 BstBI TT'CG_AA Cuts at: 1894 1894 Size: 3533 BstXI CCAn_nnnn'nTGG Cuts at: 313 313 Size: 3533 BstYI r'GATC_y Cuts at: 1 558 1025 1048 1383 1629 2641 2727 2739 Size: 557 467 23 335 246 1012 86 12 Cuts at: 2739 3085 1 Size: 346 449 Fragments arranged by size: 1012 557 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3533 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1483 1664 1827 1483 Size: 181 163 3189 Fragments arranged by size: 3189 181 163 BtsI GCAGTG_nn' Cuts at: 776 776 Size: 3533 DraI TTT'AAA Cuts at: 532 532 Size: 3533 DraIII CAC_nnn'GTG Cuts at: 289 289 Size: 3533 DrdI GACnn_nn'nnGTC Cuts at: 1239 2097 1239 Size: 858 2675 EaeI y'GGCC_r Cuts at: 110 1118 1292 1683 1710 3055 110 Size: 1008 174 391 27 1345 588 Fragments arranged by size: 1345 1008 588 391 174 27 EagI C'GGCC_G Cuts at: 1118 1118 Size: 3533 EarI CTCTTCn'nnn_ Cuts at: 391 1556 1766 391 Size: 1165 210 2158 Fragments arranged by size: 2158 1165 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1741 2051 2197 3343 1741 Size: 310 146 1146 1931 Fragments arranged by size: 1931 1146 310 146 EcoICRI GAG'CTC Cuts at: 141 545 141 Size: 404 3129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 229 1358 1790 2537 2805 229 Size: 1129 432 747 268 957 Fragments arranged by size: 1129 957 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 442 442 Size: 3533 EcoO109I rG'GnC_Cy Cuts at: 309 455 672 309 Size: 146 217 3170 Fragments arranged by size: 3170 217 146 EcoRI G'AATT_C Cuts at: 182 537 182 Size: 355 3178 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 234 441 1552 234 Size: 207 1111 2215 Fragments arranged by size: 2215 1111 207 FspI TGC'GCA Cuts at: 1314 1314 Size: 3533 HaeII r_GCGC'y Cuts at: 967 1215 2237 3216 967 Size: 248 1022 979 1284 Fragments arranged by size: 1284 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 247 251 279 283 444 476 1375 1405 1407 Size: 4 28 4 161 32 899 30 2 Cuts at: 1407 1437 247 Size: 30 2343 Fragments arranged by size: 2343 899 161 32 30 30 28 4 4 2 HincII GTy'rAC Cuts at: 491 572 491 Size: 81 3452 KasI G'GCGC_C Cuts at: 963 1211 963 Size: 248 3285 KpnI G_GTAC'C Cuts at: 553 553 Size: 3533 MluI A'CGCG_T Cuts at: 2966 2966 Size: 3533 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2204 2388 2204 Size: 184 3349 MscI TGG'CCA Cuts at: 1294 1294 Size: 3533 MslI CAynn'nnrTG Cuts at: 336 1522 1649 336 Size: 1186 127 2220 Fragments arranged by size: 2220 1186 127 MspA1I CmG'CkG Cuts at: 644 958 1318 2331 2576 644 Size: 314 360 1013 245 1601 Fragments arranged by size: 1601 1013 360 314 245 NaeI GCC'GGC Cuts at: 125 1714 125 Size: 1589 1944 NarI GG'CG_CC Cuts at: 964 1212 964 Size: 248 3285 NcoI C'CATG_G Cuts at: 107 107 Size: 3533 NgoMIV G'CCGG_C Cuts at: 123 1712 123 Size: 1589 1944 NspI r_CATG'y Cuts at: 341 586 1086 1993 341 Size: 245 500 907 1881 Fragments arranged by size: 1881 907 500 245 PciI A'CATG_T Cuts at: 337 1989 337 Size: 1652 1881 PflMI CCAn_nnn'nTGG Cuts at: 243 243 Size: 3533 PmeI GTTT'AAAC Cuts at: 532 532 Size: 3533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2672 2704 2672 Size: 32 3501 PpuMI rG'GwC_Cy Cuts at: 309 455 309 Size: 146 3387 PstI C_TGCA'G Cuts at: 580 1265 580 Size: 685 2848 PvuI CG_AT'CG Cuts at: 104 104 Size: 3533 PvuII CAG'CTG Cuts at: 958 1318 958 Size: 360 3173 RsrII CG'GwC_CG Cuts at: 1728 1728 Size: 3533 SacI G_AGCT'C Cuts at: 143 547 143 Size: 404 3129 SalI G'TCGA_C Cuts at: 570 570 Size: 3533 SapI GCTCTTCn'nnn_ Cuts at: 1556 1766 1556 Size: 210 3323 SbfI CC_TGCA'GG Cuts at: 580 580 Size: 3533 ScaI AGT'ACT Cuts at: 3050 3050 Size: 3533 SexAI A'CCwGG_T Cuts at: 305 305 Size: 3533 SfcI C'TryA_G Cuts at: 33 576 1261 2254 2445 33 Size: 543 685 993 191 1121 Fragments arranged by size: 1121 993 685 543 191 SfoI GGC'GCC Cuts at: 965 1213 965 Size: 248 3285 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3533 SmaI CCC'GGG Cuts at: 555 555 Size: 3533 SmlI C'TyrA_G Cuts at: 688 1603 2095 2357 688 Size: 915 492 262 1864 Fragments arranged by size: 1864 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3533 SphI G_CATG'C Cuts at: 586 1086 586 Size: 500 3033 StuI AGG'CCT Cuts at: 241 241 Size: 3533 StyI C'CwwG_G Cuts at: 107 242 312 667 1021 107 Size: 135 70 355 354 2619 Fragments arranged by size: 2619 355 354 135 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 250 300 1116 1249 1708 1917 250 Size: 50 816 133 459 209 1866 Fragments arranged by size: 1866 816 459 209 133 50 TatI w'GTAC_w Cuts at: 3048 3048 Size: 3533 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3050 3082 3050 Size: 32 3501 Tth111I GACn'n_nGTC Cuts at: 1330 1330 Size: 3533 XbaI T'CTAG_A Cuts at: 564 564 Size: 3533 XcmI CCAnnnn_n'nnnnTGG Cuts at: 240 1028 240 Size: 788 2745 XmaI C'CCGG_G Cuts at: 553 553 Size: 3533 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI BsaXI BsiWI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI