Restriction map for
pF1KB7436
(Circular) (Six-base) MAPSORT of: pF1KB7436.seq Check: 8483 from: 1 to: 3554
pF1KB7436 3554 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 592 592
Size: 3554
Acc65I G'GTAC_C
Cuts at: 570 570
Size: 3554
AclI AA'CG_TT
Cuts at: 3394 3394
Size: 3554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1379 1811 2558 2826 1379
Size: 432 747 268 2107
Fragments arranged by size:
2107 747 432 268
AflIII A'CryG_T
Cuts at: 906 2010 2987 906
Size: 1104 977 1473
Fragments arranged by size:
1473 1104 977
AgeI A'CCGG_T
Cuts at: 1942 1942
Size: 3554
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 204 236 204
Size: 32 3522
AlwNI CAG_nnn'CTG
Cuts at: 859 2426 859
Size: 1567 1987
ApaLI G'TGCA_C
Cuts at: 394 2324 394
Size: 1930 1624
ApoI r'AATT_y
Cuts at: 359 558 2907 3163 359
Size: 199 2349 256 750
Fragments arranged by size:
2349 750 256 199
AseI AT'TA_AT
Cuts at: 20 20
Size: 3554
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3554
AvaI C'yCGr_G
Cuts at: 487 574 487
Size: 87 3467
BamHI G'GATC_C
Cuts at: 579 579
Size: 3554
BanI G'GyrC_C
Cuts at: 570 984 1232 1267 570
Size: 414 248 35 2857
Fragments arranged by size:
2857 414 248 35
BanII G_rGCy'C
Cuts at: 568 1598 568
Size: 1030 2524
BbeI G_GCGC'C
Cuts at: 988 1236 988
Size: 248 3306
BbsI GAAGACnn'nnnn_
Cuts at: 551 551
Size: 3554
BciVI GTATCCnnnnn_n'
Cuts at: 1452 2213 3554 1452
Size: 761 1341 1452
Fragments arranged by size:
1452 1341 761
BclI T'GATC_A
Cuts at: 1074 1074
Size: 3554
BglII A'GATC_T
Cuts at: 1069 3106 1069
Size: 2037 1517
BlpI GC'TnA_GC
Cuts at: 666 666
Size: 3554
Bme1580I G_kGCm'C
Cuts at: 398 513 1179 1272 2328 398
Size: 115 666 93 1056 1624
Fragments arranged by size:
1624 1056 666 115 93
BmrI ACTGGGnnnn_n'
Cuts at: 128 766 847 1182 3326 128
Size: 638 81 335 2144 356
Fragments arranged by size:
2144 638 356 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1921 1921
Size: 3554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 309 162
Size: 147 3407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 730 1609 2101 2399 268
Size: 462 879 492 298 1423
Fragments arranged by size:
1423 879 492 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 3258 3258
Size: 3554
BsaAI yAC'GTr
Cuts at: 907 907
Size: 3554
BsaBI GATnn'nnATC
Cuts at: 6 1068 3105 6
Size: 1062 2037 455
Fragments arranged by size:
2037 1062 455
BsaHI Gr'CG_yC
Cuts at: 985 1233 1935 3431 985
Size: 248 702 1496 1108
Fragments arranged by size:
1496 1108 702 248
BsaWI w'CCGG_w
Cuts at: 743 1264 1942 2216 2363 2774 743
Size: 521 678 274 147 411 1523
Fragments arranged by size:
1523 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 204 234 204
Size: 30 3524
BseYI C'CCAG_C
Cuts at: 979 1737 2314 2810 979
Size: 758 577 496 1723
Fragments arranged by size:
1723 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1096 1142 2350 104
Size: 992 46 1208 1308
Fragments arranged by size:
1308 1208 992 46
BsiHKAI G_wGCw'C
Cuts at: 398 568 1346 1536 2328 398
Size: 170 778 190 792 1624
Fragments arranged by size:
1624 792 778 190 170
Bsp1286I G_dGCh'C
Cuts at: 398 513 568 1179 1272 1346 1536 1598 2328
Size: 115 55 611 93 74 190 62 730
Cuts at: 2328 398
Size: 1624
Fragments arranged by size:
1624 730 611 190 115 93 74 62
55
BspEI T'CCGG_A
Cuts at: 2774 2774
Size: 3554
BspHI T'CATG_A
Cuts at: 537 2730 537
Size: 2193 1361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 431 604 818 1120 1501 431
Size: 173 214 302 381 2484
Fragments arranged by size:
2484 381 302 214 173
BsrBI CCG'CTC
Cuts at: 50 1846 1900 3384 3549 50
Size: 1796 54 1484 165 55
Fragments arranged by size:
1796 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1466 1466
Size: 3554
BsrFI r'CCGG_y
Cuts at: 1552 1733 1942 1552
Size: 181 209 3164
Fragments arranged by size:
3164 209 181
BssSI C'ACGA_G
Cuts at: 1825 2183 1825
Size: 358 3196
BstAPI GCAn_nnn'nTGC
Cuts at: 170 931 170
Size: 761 2793
BstBI TT'CG_AA
Cuts at: 1915 1915
Size: 3554
BstYI r'GATC_y
Cuts at: 1 267 579 1046 1069 1404 1650 2662 2748
Size: 266 312 467 23 335 246 1012 86
Cuts at: 2748 2760 3106 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 312 266 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 498 107
Size: 391 3163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1504 1685 1848 1504
Size: 181 163 3210
Fragments arranged by size:
3210 181 163
BtsI GCAGTG_nn'
Cuts at: 797 797
Size: 3554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 105 140 105
Size: 35 3519
DraI TTT'AAA
Cuts at: 553 553
Size: 3554
DraIII CAC_nnn'GTG
Cuts at: 328 328
Size: 3554
DrdI GACnn_nn'nnGTC
Cuts at: 1260 2118 1260
Size: 858 2696
EaeI y'GGCC_r
Cuts at: 528 1139 1313 1704 1731 3076 528
Size: 611 174 391 27 1345 1006
Fragments arranged by size:
1345 1006 611 391 174 27
EagI C'GGCC_G
Cuts at: 1139 1139
Size: 3554
EarI CTCTTCn'nnn_
Cuts at: 1577 1787 1577
Size: 210 3344
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1762 2072 2218 3364 1762
Size: 310 146 1146 1952
Fragments arranged by size:
1952 1146 310 146
EcoICRI GAG'CTC
Cuts at: 566 566
Size: 3554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 309 1379 1811 2558 2826 162
Size: 147 1070 432 747 268 890
Fragments arranged by size:
1070 890 747 432 268 147
EcoO109I rG'GnC_Cy
Cuts at: 693 693
Size: 3554
EcoRI G'AATT_C
Cuts at: 558 558
Size: 3554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1573 1573
Size: 3554
FspI TGC'GCA
Cuts at: 1335 1335
Size: 3554
HaeII r_GCGC'y
Cuts at: 988 1236 2258 3237 988
Size: 248 1022 979 1305
Fragments arranged by size:
1305 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1396 1426 1428 1458 1396
Size: 30 2 30 3492
Fragments arranged by size:
3492 30 30 2
HincII GTy'rAC
Cuts at: 593 593
Size: 3554
KasI G'GCGC_C
Cuts at: 984 1232 984
Size: 248 3306
KpnI G_GTAC'C
Cuts at: 574 574
Size: 3554
MluI A'CGCG_T
Cuts at: 2987 2987
Size: 3554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2225 2409 2225
Size: 184 3370
MscI TGG'CCA
Cuts at: 530 1315 530
Size: 785 2769
MslI CAynn'nnrTG
Cuts at: 536 1543 1670 536
Size: 1007 127 2420
Fragments arranged by size:
2420 1007 127
MspA1I CmG'CkG
Cuts at: 665 979 1339 2352 2597 665
Size: 314 360 1013 245 1622
Fragments arranged by size:
1622 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1735 1735
Size: 3554
NarI GG'CG_CC
Cuts at: 985 1233 985
Size: 248 3306
NcoI C'CATG_G
Cuts at: 107 107
Size: 3554
NgoMIV G'CCGG_C
Cuts at: 1733 1733
Size: 3554
NspI r_CATG'y
Cuts at: 219 607 1107 2014 219
Size: 388 500 907 1759
Fragments arranged by size:
1759 907 500 388
PciI A'CATG_T
Cuts at: 2010 2010
Size: 3554
PfoI T'CCnGG_A
Cuts at: 139 139
Size: 3554
PmeI GTTT'AAAC
Cuts at: 553 553
Size: 3554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 204 236 2693 2725 204
Size: 32 2457 32 1033
Fragments arranged by size:
2457 1033 32 32
PstI C_TGCA'G
Cuts at: 601 1286 601
Size: 685 2869
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3554
PvuII CAG'CTG
Cuts at: 979 1339 979
Size: 360 3194
RsrII CG'GwC_CG
Cuts at: 1749 1749
Size: 3554
SacI G_AGCT'C
Cuts at: 568 568
Size: 3554
SalI G'TCGA_C
Cuts at: 591 591
Size: 3554
SapI GCTCTTCn'nnn_
Cuts at: 1577 1787 1577
Size: 210 3344
SbfI CC_TGCA'GG
Cuts at: 601 601
Size: 3554
ScaI AGT'ACT
Cuts at: 3071 3071
Size: 3554
SfcI C'TryA_G
Cuts at: 33 597 1282 2275 2466 33
Size: 564 685 993 191 1121
Fragments arranged by size:
1121 993 685 564 191
SfoI GGC'GCC
Cuts at: 986 1234 986
Size: 248 3306
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3554
SmaI CCC'GGG
Cuts at: 576 576
Size: 3554
SmlI C'TyrA_G
Cuts at: 247 487 709 1624 2116 2378 247
Size: 240 222 915 492 262 1423
Fragments arranged by size:
1423 915 492 262 240 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3554
SphI G_CATG'C
Cuts at: 607 1107 607
Size: 500 3054
SspI AAT'ATT
Cuts at: 186 186
Size: 3554
StyI C'CwwG_G
Cuts at: 107 375 688 1042 107
Size: 268 313 354 2619
Fragments arranged by size:
2619 354 313 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1137 1270 1729 1938 1137
Size: 133 459 209 2753
Fragments arranged by size:
2753 459 209 133
TatI w'GTAC_w
Cuts at: 3069 3069
Size: 3554
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3071 3103 3071
Size: 32 3522
Tth111I GACn'n_nGTC
Cuts at: 1351 1351
Size: 3554
XbaI T'CTAG_A
Cuts at: 585 585
Size: 3554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1049 1049
Size: 3554
XhoI C'TCGA_G
Cuts at: 487 487
Size: 3554
XmaI C'CCGG_G
Cuts at: 574 574
Size: 3554
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XmnI ZraI