FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KB7437, 238 aa 1>>>pF1KB7437 238 - 238 aa - 238 aa Library: /omim/omim.rfq.tfa 60827320 residues in 85289 sequences Statistics: Expectation_n fit: rho(ln(x))= 4.4950+/-0.000505; mu= 18.6443+/- 0.031 mean_var=68.2688+/-14.658, 0's: 0 Z-trim(107.4): 60 B-trim: 0 in 0/48 Lambda= 0.155226 statistics sampled from 15460 (15504) to 15460 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.522), E-opt: 0.2 (0.182), width: 16 Scan time: 5.700 The best scores are: opt bits E(85289) NP_057140 (OMIM: 616874) protein lifeguard 4 isofo ( 238) 1497 344.6 8.6e-95 NP_001269535 (OMIM: 616874) protein lifeguard 4 is ( 285) 1275 295.0 9.1e-80 NP_001269539 (OMIM: 616874) protein lifeguard 4 is ( 207) 1205 279.2 3.8e-75 NP_001269538 (OMIM: 616874) protein lifeguard 4 is ( 162) 970 226.5 2.2e-59 NP_000828 (OMIM: 138251) protein lifeguard 1 [Homo ( 371) 398 98.7 1.4e-20 NP_001009184 (OMIM: 138251) protein lifeguard 1 [H ( 371) 398 98.7 1.4e-20 XP_016874529 (OMIM: 604306) PREDICTED: protein lif ( 316) 375 93.5 4.6e-19 NP_036438 (OMIM: 604306) protein lifeguard 2 [Homo ( 316) 375 93.5 4.6e-19 XP_005268787 (OMIM: 604306) PREDICTED: protein lif ( 274) 373 93.0 5.7e-19 NP_001308357 (OMIM: 610364) protein lifeguard 3 is ( 311) 284 73.1 6.2e-13 NP_001308361 (OMIM: 610364) protein lifeguard 3 is ( 311) 284 73.1 6.2e-13 NP_001308364 (OMIM: 610364) protein lifeguard 3 is ( 311) 284 73.1 6.2e-13 NP_001308358 (OMIM: 610364) protein lifeguard 3 is ( 311) 284 73.1 6.2e-13 NP_001308365 (OMIM: 610364) protein lifeguard 3 is ( 311) 284 73.1 6.2e-13 NP_071435 (OMIM: 610364) protein lifeguard 3 isofo ( 311) 284 73.1 6.2e-13 NP_001308359 (OMIM: 610364) protein lifeguard 3 is ( 311) 284 73.1 6.2e-13 NP_001308356 (OMIM: 610364) protein lifeguard 3 is ( 311) 284 73.1 6.2e-13 NP_001308362 (OMIM: 610364) protein lifeguard 3 is ( 311) 284 73.1 6.2e-13 XP_011509929 (OMIM: 610364) PREDICTED: protein lif ( 311) 284 73.1 6.2e-13 NP_001308367 (OMIM: 610364) protein lifeguard 3 is ( 200) 269 69.6 4.7e-12 NP_003208 (OMIM: 600748) bax inhibitor 1 isoform 1 ( 237) 226 60.0 4.2e-09 NP_001092046 (OMIM: 600748) bax inhibitor 1 isofor ( 295) 226 60.1 4.8e-09 XP_005269183 (OMIM: 600748) PREDICTED: bax inhibit ( 295) 226 60.1 4.8e-09 >>NP_057140 (OMIM: 616874) protein lifeguard 4 isoform b (238 aa) initn: 1497 init1: 1497 opt: 1497 Z-score: 1822.8 bits: 344.6 E(85289): 8.6e-95 Smith-Waterman score: 1497; 100.0% identity (100.0% similar) in 238 aa overlap (1-238:1-238) 10 20 30 40 50 60 pF1KB7 MADPDPRYPRSSIEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYFE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_057 MADPDPRYPRSSIEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYFE 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB7 SVRTFVHESPALILLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_057 SVRTFVHESPALILLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYD 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB7 VYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSEIMEL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_057 VYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSEIMEL 130 140 150 160 170 180 190 200 210 220 230 pF1KB7 VLAAAGALLFCGFIIYDTHSLMHKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_057 VLAAAGALLFCGFIIYDTHSLMHKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK 190 200 210 220 230 >>NP_001269535 (OMIM: 616874) protein lifeguard 4 isofor (285 aa) initn: 1275 init1: 1275 opt: 1275 Z-score: 1553.1 bits: 295.0 E(85289): 9.1e-80 Smith-Waterman score: 1279; 82.2% identity (82.6% similar) in 270 aa overlap (16-238:16-285) 10 20 30 pF1KB7 MADPDPRYPRSSIEDDFNYGSSVASATVHIRMA--------------------------- :::::::::::::::::. NP_001 MADPDPRYPRSSIEDDFNYGSSVASATVHIRMVLQRDCTFSKQYMRIPSAPSATLNIIRF 10 20 30 40 50 60 40 50 60 70 pF1KB7 --------------------FLRKVYSILSLQVLLTTVTSTVFLYFESVRTFVHESPALI :::::::::::::::::::::::::::::::::::::::: NP_001 FHLRQSGRSGMVNIFYKGPTFLRKVYSILSLQVLLTTVTSTVFLYFESVRTFVHESPALI 70 80 90 100 110 120 80 90 100 110 120 130 pF1KB7 LLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYDVYIILQAFILTTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 LLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYDVYIILQAFILTTT 130 140 150 160 170 180 140 150 160 170 180 190 pF1KB7 VFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSEIMELVLAAAGALLFCGF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 VFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSEIMELVLAAAGALLFCGF 190 200 210 220 230 240 200 210 220 230 pF1KB7 IIYDTHSLMHKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK ::::::::::::::::::::::::::::::::::::::::::::: NP_001 IIYDTHSLMHKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK 250 260 270 280 >>NP_001269539 (OMIM: 616874) protein lifeguard 4 isofor (207 aa) initn: 1205 init1: 1205 opt: 1205 Z-score: 1470.1 bits: 279.2 E(85289): 3.8e-75 Smith-Waterman score: 1205; 99.0% identity (99.0% similar) in 196 aa overlap (43-238:12-207) 20 30 40 50 60 70 pF1KB7 IEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYFESVRTFVHESPAL : ::::::::::::::::::::::::::: NP_001 MAAAWPPPPCTSEWVLLTTVTSTVFLYFESVRTFVHESPAL 10 20 30 40 80 90 100 110 120 130 pF1KB7 ILLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYDVYIILQAFILTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 ILLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYDVYIILQAFILTT 50 60 70 80 90 100 140 150 160 170 180 190 pF1KB7 TVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSEIMELVLAAAGALLFCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 TVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSEIMELVLAAAGALLFCG 110 120 130 140 150 160 200 210 220 230 pF1KB7 FIIYDTHSLMHKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK :::::::::::::::::::::::::::::::::::::::::::::: NP_001 FIIYDTHSLMHKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK 170 180 190 200 >>NP_001269538 (OMIM: 616874) protein lifeguard 4 isofor (162 aa) initn: 994 init1: 970 opt: 970 Z-score: 1187.0 bits: 226.5 E(85289): 2.2e-59 Smith-Waterman score: 970; 95.7% identity (99.4% similar) in 162 aa overlap (1-162:1-162) 10 20 30 40 50 60 pF1KB7 MADPDPRYPRSSIEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYFE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 MADPDPRYPRSSIEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYFE 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB7 SVRTFVHESPALILLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 SVRTFVHESPALILLFALGSLGLIFALILNRHKYPLNLYLLFGFTLLEALTVAVVVTFYD 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB7 VYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSEIMEL :::::::::::::::::::::::::::::::::::.. .... NP_001 VYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGILEVFFL 130 140 150 160 190 200 210 220 230 pF1KB7 VLAAAGALLFCGFIIYDTHSLMHKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK >>NP_000828 (OMIM: 138251) protein lifeguard 1 [Homo sap (371 aa) initn: 418 init1: 211 opt: 398 Z-score: 490.2 bits: 98.7 E(85289): 1.4e-20 Smith-Waterman score: 398; 35.2% identity (69.5% similar) in 210 aa overlap (30-232:157-366) 10 20 30 40 50 pF1KB7 MADPDPRYPRSSIEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYF ::.::.:::. .:.::. .: : .:: . NP_000 SPQHGNYQEEGPPSYYDNQDFPATNWDDKSIRQAFIRKVFLVLTLQLSVTLSTVSVFTFV 130 140 150 160 170 180 60 70 80 90 100 110 pF1KB7 ESVRTFVHESP-ALILLFALGSLGLIFALILN--RHKYPLNLYLLFGFTLLEALTVAVVV :. ::.:. . . .:. ..:: . :.:.: :: : .: . :.... NP_000 AEVKGFVRENVWTYYVSYAVFFISLIVLSCCGDFRRKHPWNLVALSVLTASLSYMVGMIA 190 200 210 220 230 240 120 130 140 150 160 170 pF1KB7 TFYDVYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSE .::.. ...: .::.: : ......:.. ::.. . :.. . .: . ..: .:. .. NP_000 SFYNTEAVIMAVGITTAVCFTVVIFSMQTRYDFTSCMGVLLVSMVVLFIFAILCIFIRNR 250 260 270 280 290 300 180 190 200 210 220 230 pF1KB7 IMELVLAAAGALLFCGFIIYDTHSLMHK----LSPEEYVLAAISLYLDIINLFLHLLRFL :.:.: :. ::::: :. ::. :. . :::::::.::..:: ::::.::..: .. NP_000 ILEIVYASLGALLFTCFLAVDTQLLLGNKQLSLSPEEYVFAALNLYTDIINIFLYILTII 310 320 330 340 350 360 pF1KB7 EAVNKK NP_000 GRAKE 370 >>NP_001009184 (OMIM: 138251) protein lifeguard 1 [Homo (371 aa) initn: 418 init1: 211 opt: 398 Z-score: 490.2 bits: 98.7 E(85289): 1.4e-20 Smith-Waterman score: 398; 35.2% identity (69.5% similar) in 210 aa overlap (30-232:157-366) 10 20 30 40 50 pF1KB7 MADPDPRYPRSSIEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYF ::.::.:::. .:.::. .: : .:: . NP_001 SPQHGNYQEEGPPSYYDNQDFPATNWDDKSIRQAFIRKVFLVLTLQLSVTLSTVSVFTFV 130 140 150 160 170 180 60 70 80 90 100 110 pF1KB7 ESVRTFVHESP-ALILLFALGSLGLIFALILN--RHKYPLNLYLLFGFTLLEALTVAVVV :. ::.:. . . .:. ..:: . :.:.: :: : .: . :.... NP_001 AEVKGFVRENVWTYYVSYAVFFISLIVLSCCGDFRRKHPWNLVALSVLTASLSYMVGMIA 190 200 210 220 230 240 120 130 140 150 160 170 pF1KB7 TFYDVYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGFLKFFFYSE .::.. ...: .::.: : ......:.. ::.. . :.. . .: . ..: .:. .. NP_001 SFYNTEAVIMAVGITTAVCFTVVIFSMQTRYDFTSCMGVLLVSMVVLFIFAILCIFIRNR 250 260 270 280 290 300 180 190 200 210 220 230 pF1KB7 IMELVLAAAGALLFCGFIIYDTHSLMHK----LSPEEYVLAAISLYLDIINLFLHLLRFL :.:.: :. ::::: :. ::. :. . :::::::.::..:: ::::.::..: .. NP_001 ILEIVYASLGALLFTCFLAVDTQLLLGNKQLSLSPEEYVFAALNLYTDIINIFLYILTII 310 320 330 340 350 360 pF1KB7 EAVNKK NP_001 GRAKE 370 >>XP_016874529 (OMIM: 604306) PREDICTED: protein lifegua (316 aa) initn: 342 init1: 135 opt: 375 Z-score: 463.3 bits: 93.5 E(85289): 4.6e-19 Smith-Waterman score: 375; 33.1% identity (62.9% similar) in 248 aa overlap (5-232:69-311) 10 20 pF1KB7 MADPDPRYPRSSIEDDFNYGSSVASATV-----H :: :: .. : :. .: . XP_016 EEATSGEGMKAGAFPPAPTAVPLHPSWAYVDPS-SSSSYDNGFPTGDHELFTTFSWDDQK 40 50 60 70 80 90 30 40 50 60 70 80 pF1KB7 IRMAFLRKVYSILSLQVLLTTVTSTVFLYFESVRTFVHESPALILLFALGSLGLIFALIL .: .:.::::.:: .:.:.: .. ..: . . :. .:. .:. . .: ...:: : XP_016 VRRVFVRKVYTILLIQLLVTLAVVALFTFCDPVKDYVQANPG----WYWASYAVFFATYL 100 110 120 130 140 150 90 100 110 120 130 140 pF1KB7 N-------RHKYPLNLYLLFGFTLLEALTVAVVVTFYDVYIILQAFILTTTVFFGLTVYT . :...: :: :: ::: : .... ..:.. .: . .:. : ...::.. XP_016 TLACCSGPRRHFPWNLILLTVFTLSMAYLTGMLSSYYNTTSVLLCLGITALVCLSVTVFS 160 170 180 190 200 210 150 160 170 180 190 pF1KB7 LQSKKDFSKFGAGLFALLWILCLSGF----LKFFFYSEIMELVLAAAGALLFCGFIIYDT .:.: ::.. . ::.:: : .::. : : : .. : :: :: .: :. :: XP_016 FQTKFDFTSCQGVLFVLLMTLFFSGLILAILLPFQYVPWLHAVYAALGAGVFTLFLALDT 220 230 240 250 260 270 200 210 220 230 pF1KB7 HSLM----HKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK . :: :.::::::...:...::::: .: .:... XP_016 QLLMGNRRHSLSPEEYIFGALNIYLDIIYIFTFFLQLFGTNRE 280 290 300 310 >>NP_036438 (OMIM: 604306) protein lifeguard 2 [Homo sap (316 aa) initn: 342 init1: 135 opt: 375 Z-score: 463.3 bits: 93.5 E(85289): 4.6e-19 Smith-Waterman score: 375; 33.1% identity (62.9% similar) in 248 aa overlap (5-232:69-311) 10 20 pF1KB7 MADPDPRYPRSSIEDDFNYGSSVASATV-----H :: :: .. : :. .: . NP_036 EEATSGEGMKAGAFPPAPTAVPLHPSWAYVDPS-SSSSYDNGFPTGDHELFTTFSWDDQK 40 50 60 70 80 90 30 40 50 60 70 80 pF1KB7 IRMAFLRKVYSILSLQVLLTTVTSTVFLYFESVRTFVHESPALILLFALGSLGLIFALIL .: .:.::::.:: .:.:.: .. ..: . . :. .:. .:. . .: ...:: : NP_036 VRRVFVRKVYTILLIQLLVTLAVVALFTFCDPVKDYVQANPG----WYWASYAVFFATYL 100 110 120 130 140 150 90 100 110 120 130 140 pF1KB7 N-------RHKYPLNLYLLFGFTLLEALTVAVVVTFYDVYIILQAFILTTTVFFGLTVYT . :...: :: :: ::: : .... ..:.. .: . .:. : ...::.. NP_036 TLACCSGPRRHFPWNLILLTVFTLSMAYLTGMLSSYYNTTSVLLCLGITALVCLSVTVFS 160 170 180 190 200 210 150 160 170 180 190 pF1KB7 LQSKKDFSKFGAGLFALLWILCLSGF----LKFFFYSEIMELVLAAAGALLFCGFIIYDT .:.: ::.. . ::.:: : .::. : : : .. : :: :: .: :. :: NP_036 FQTKFDFTSCQGVLFVLLMTLFFSGLILAILLPFQYVPWLHAVYAALGAGVFTLFLALDT 220 230 240 250 260 270 200 210 220 230 pF1KB7 HSLM----HKLSPEEYVLAAISLYLDIINLFLHLLRFLEAVNKK . :: :.::::::...:...::::: .: .:... NP_036 QLLMGNRRHSLSPEEYIFGALNIYLDIIYIFTFFLQLFGTNRE 280 290 300 310 >>XP_005268787 (OMIM: 604306) PREDICTED: protein lifegua (274 aa) initn: 342 init1: 135 opt: 373 Z-score: 461.6 bits: 93.0 E(85289): 5.7e-19 Smith-Waterman score: 373; 34.4% identity (66.1% similar) in 218 aa overlap (30-232:56-269) 10 20 30 40 50 pF1KB7 MADPDPRYPRSSIEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYF .: .:.::::.:: .:.:.: .. ..: . XP_005 VNEGSSSSYDNGFPTGDHELFTTFSWDDQKVRRVFVRKVYTILLIQLLVTLAVVALFTFC 30 40 50 60 70 80 60 70 80 90 100 110 pF1KB7 ESVRTFVHESPALILLFALGSLGLIFALILN-------RHKYPLNLYLLFGFTLLEALTV . :. .:. .:. . .: ...:: :. :...: :: :: ::: : . XP_005 DPVKDYVQANPG----WYWASYAVFFATYLTLACCSGPRRHFPWNLILLTVFTLSMAYLT 90 100 110 120 130 140 120 130 140 150 160 pF1KB7 AVVVTFYDVYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGLFALLWILCLSGF---- ... ..:.. .: . .:. : ...::...:.: ::.. . ::.:: : .::. XP_005 GMLSSYYNTTSVLLCLGITALVCLSVTVFSFQTKFDFTSCQGVLFVLLMTLFFSGLILAI 150 160 170 180 190 200 170 180 190 200 210 220 pF1KB7 LKFFFYSEIMELVLAAAGALLFCGFIIYDTHSLM----HKLSPEEYVLAAISLYLDIINL : : : .. : :: :: .: :. ::. :: :.::::::...:...::::: . XP_005 LLPFQYVPWLHAVYAALGAGVFTLFLALDTQLLMGNRRHSLSPEEYIFGALNIYLDIIYI 210 220 230 240 250 260 230 pF1KB7 FLHLLRFLEAVNKK : .:... XP_005 FTFFLQLFGTNRE 270 >>NP_001308357 (OMIM: 610364) protein lifeguard 3 isofor (311 aa) initn: 233 init1: 120 opt: 284 Z-score: 353.2 bits: 73.1 E(85289): 6.2e-13 Smith-Waterman score: 362; 31.3% identity (68.7% similar) in 217 aa overlap (30-232:94-307) 10 20 30 40 50 pF1KB7 MADPDPRYPRSSIEDDFNYGSSVASATVHIRMAFLRKVYSILSLQVLLTTVTSTVFLYF .: .:.::::::.:.:.:.:.. ..: . NP_001 PMNYGPGHGYDGEERAVSDSFGPGEWDDRKVRHTFIRKVYSIISVQLLITVAIIAIFTFV 70 80 90 100 110 120 60 70 80 90 100 110 pF1KB7 ESVRTFVHESPALILLFALGSLGLIFALILN-----RHKYPLNLYLLFGFTLLEALTVAV : : .::... :. .. .. .. ::: :...: :. :: ::. .. ... NP_001 EPVSAFVRRNVAVY--YVSYAVFVVTYLILACCQGPRRRFPWNIILLTLFTFAMGFMTGT 130 140 150 160 170 180 120 130 140 150 160 170 pF1KB7 VVTFYDVYIILQAFILTTTVFFGLTVYTLQSKKDFSKFGAGLFALLWILCL-SGFLK--- . ..:.. .. :.:.:..: ...:.. .:.: ::.. .::: .: :. : .:.. NP_001 ISSMYQTKAVIIAMIITAVVSISVTIFCFQTKVDFTSC-TGLFCVLGIVLLVTGIVTSIV 190 200 210 220 230 240 180 190 200 210 220 pF1KB7 -FFFYSEIMELVLAAAGALLFCGFIIYDTHSLM----HKLSPEEYVLAAISLYLDIINLF .: : .... :: ::. : :. :::. .. : .:::.:. .:...: ::: .: NP_001 LYFQYVYWLHMLYAALGAICFTLFLAYDTQLVLGNRKHTISPEDYITGALQIYTDIIYIF 250 260 270 280 290 300 230 pF1KB7 LHLLRFLEAVNKK .:... NP_001 TFVLQLMGDRN 310 238 residues in 1 query sequences 60827320 residues in 85289 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Fri Nov 4 07:48:18 2016 done: Fri Nov 4 07:48:19 2016 Total Scan time: 5.700 Total Display time: 0.010 Function used was FASTA [36.3.4 Apr, 2011]