Restriction map for
pF1KB7437
(Circular) (Six-base) MAPSORT of: pF1KB7437.seq Check: 7117 from: 1 to: 3827
pF1KB7437 3827 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 221 529 865 221
Size: 308 336 3183
Fragments arranged by size:
3183 336 308
Acc65I G'GTAC_C
Cuts at: 128 843 128
Size: 715 3112
AclI AA'CG_TT
Cuts at: 3667 3667
Size: 3827
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 1652 2084 2831 3099 749
Size: 903 432 747 268 1477
Fragments arranged by size:
1477 903 747 432 268
AflIII A'CryG_T
Cuts at: 1179 2283 3260 1179
Size: 1104 977 1746
Fragments arranged by size:
1746 1104 977
AgeI A'CCGG_T
Cuts at: 2215 2215
Size: 3827
AlwNI CAG_nnn'CTG
Cuts at: 1132 2699 1132
Size: 1567 2260
ApaLI G'TGCA_C
Cuts at: 190 2597 190
Size: 2407 1420
ApoI r'AATT_y
Cuts at: 559 831 3180 3436 559
Size: 272 2349 256 950
Fragments arranged by size:
2349 950 272 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3827
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3827
AvaI C'yCGr_G
Cuts at: 847 847
Size: 3827
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 370 403 370
Size: 33 3794
BamHI G'GATC_C
Cuts at: 852 852
Size: 3827
BanI G'GyrC_C
Cuts at: 128 843 1257 1505 1540 128
Size: 715 414 248 35 2415
Fragments arranged by size:
2415 715 414 248 35
BanII G_rGCy'C
Cuts at: 669 841 1871 669
Size: 172 1030 2625
Fragments arranged by size:
2625 1030 172
BbeI G_GCGC'C
Cuts at: 1261 1509 1261
Size: 248 3579
BciVI GTATCCnnnnn_n'
Cuts at: 398 1725 2486 3827 398
Size: 1327 761 1341 398
Fragments arranged by size:
1341 1327 761 398
BclI T'GATC_A
Cuts at: 1347 1347
Size: 3827
BfrBI ATG'CAT
Cuts at: 714 714
Size: 3827
BglII A'GATC_T
Cuts at: 1342 3379 1342
Size: 2037 1790
BlpI GC'TnA_GC
Cuts at: 939 939
Size: 3827
Bme1580I G_kGCm'C
Cuts at: 194 1452 1545 2601 194
Size: 1258 93 1056 1420
Fragments arranged by size:
1420 1258 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1039 1120 1455 3599 1039
Size: 81 335 2144 1267
Fragments arranged by size:
2144 1267 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 3827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1882 2374 2672 1003
Size: 879 492 298 2158
Fragments arranged by size:
2158 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3531 3531
Size: 3827
BsaAI yAC'GTr
Cuts at: 738 1180 738
Size: 442 3385
BsaBI GATnn'nnATC
Cuts at: 6 1341 3378 6
Size: 1335 2037 455
Fragments arranged by size:
2037 1335 455
BsaHI Gr'CG_yC
Cuts at: 1258 1506 2208 3704 1258
Size: 248 702 1496 1381
Fragments arranged by size:
1496 1381 702 248
BsaWI w'CCGG_w
Cuts at: 1016 1537 2215 2489 2636 3047 1016
Size: 521 678 274 147 411 1796
Fragments arranged by size:
1796 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 121 151 121
Size: 30 3797
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 129 129
Size: 3827
BseYI C'CCAG_C
Cuts at: 1252 2010 2587 3083 1252
Size: 758 577 496 1996
Fragments arranged by size:
1996 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 769
Size: 3827
BsiEI CG_ry'CG
Cuts at: 104 146 1369 1415 2623 104
Size: 42 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46 42
BsiHKAI G_wGCw'C
Cuts at: 194 841 1619 1809 2601 194
Size: 647 778 190 792 1420
Fragments arranged by size:
1420 792 778 647 190
BsmI GAATG_Cn'
Cuts at: 227 227
Size: 3827
Bsp1286I G_dGCh'C
Cuts at: 194 669 841 1452 1545 1619 1809 1871 2601
Size: 475 172 611 93 74 190 62 730
Cuts at: 2601 194
Size: 1420
Fragments arranged by size:
1420 730 611 475 190 172 93 74
62
BspEI T'CCGG_A
Cuts at: 3047 3047
Size: 3827
BspHI T'CATG_A
Cuts at: 3003 3003
Size: 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 231 877 1091 1393 1774 231
Size: 646 214 302 381 2284
Fragments arranged by size:
2284 646 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2119 2173 3657 3822 50
Size: 2069 54 1484 165 55
Fragments arranged by size:
2069 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1739 1739
Size: 3827
BsrFI r'CCGG_y
Cuts at: 1825 2006 2215 1825
Size: 181 209 3437
Fragments arranged by size:
3437 209 181
BsrGI T'GTAC_A
Cuts at: 303 303
Size: 3827
BssSI C'ACGA_G
Cuts at: 2098 2456 2098
Size: 358 3469
BstAPI GCAn_nnn'nTGC
Cuts at: 1204 1204
Size: 3827
BstBI TT'CG_AA
Cuts at: 2188 2188
Size: 3827
BstYI r'GATC_y
Cuts at: 1 343 852 1319 1342 1677 1923 2935 3021
Size: 342 509 467 23 335 246 1012 86
Cuts at: 3021 3033 3379 1
Size: 12 346 449
Fragments arranged by size:
1012 509 467 449 346 342 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 530 530
Size: 3827
BtgI C'CryG_G
Cuts at: 107 107
Size: 3827
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1777 1958 2121 1777
Size: 181 163 3483
Fragments arranged by size:
3483 181 163
BtsI GCAGTG_nn'
Cuts at: 1070 1070
Size: 3827
DraI TTT'AAA
Cuts at: 374 826 374
Size: 452 3375
DrdI GACnn_nn'nnGTC
Cuts at: 1533 2391 1533
Size: 858 2969
EaeI y'GGCC_r
Cuts at: 1412 1586 1977 2004 3349 1412
Size: 174 391 27 1345 1890
Fragments arranged by size:
1890 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1412 1412
Size: 3827
EarI CTCTTCn'nnn_
Cuts at: 725 1850 2060 725
Size: 1125 210 2492
Fragments arranged by size:
2492 1125 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 171 2035 2345 2491 3637 171
Size: 1864 310 146 1146 361
Fragments arranged by size:
1864 1146 361 310 146
EcoICRI GAG'CTC
Cuts at: 839 839
Size: 3827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 1652 2084 2831 3099 749
Size: 903 432 747 268 1477
Fragments arranged by size:
1477 903 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 966 966
Size: 3827
EcoRI G'AATT_C
Cuts at: 831 831
Size: 3827
EcoRV GAT'ATC
Cuts at: 768 768
Size: 3827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1846 1846
Size: 3827
FspI TGC'GCA
Cuts at: 1608 1608
Size: 3827
HaeII r_GCGC'y
Cuts at: 1261 1509 2531 3510 1261
Size: 248 1022 979 1578
Fragments arranged by size:
1578 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 674 688 706 720 1669 1699 1701 1731 674
Size: 14 18 14 949 30 2 30 2770
Fragments arranged by size:
2770 949 30 30 18 14 14 2
HincII GTy'rAC
Cuts at: 866 866
Size: 3827
HindIII A'AGCT_T
Cuts at: 485 485
Size: 3827
KasI G'GCGC_C
Cuts at: 1257 1505 1257
Size: 248 3579
KpnI G_GTAC'C
Cuts at: 132 847 132
Size: 715 3112
MluI A'CGCG_T
Cuts at: 3260 3260
Size: 3827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 407 2498 2682 407
Size: 2091 184 1552
Fragments arranged by size:
2091 1552 184
MscI TGG'CCA
Cuts at: 1588 1588
Size: 3827
MslI CAynn'nnrTG
Cuts at: 199 1816 1943 199
Size: 1617 127 2083
Fragments arranged by size:
2083 1617 127
MspA1I CmG'CkG
Cuts at: 658 938 1252 1612 2625 2870 658
Size: 280 314 360 1013 245 1615
Fragments arranged by size:
1615 1013 360 314 280 245
NaeI GCC'GGC
Cuts at: 2008 2008
Size: 3827
NarI GG'CG_CC
Cuts at: 1258 1506 1258
Size: 248 3579
NcoI C'CATG_G
Cuts at: 107 107
Size: 3827
NgoMIV G'CCGG_C
Cuts at: 2006 2006
Size: 3827
NsiI A_TGCA'T
Cuts at: 716 716
Size: 3827
NspI r_CATG'y
Cuts at: 880 1380 2287 880
Size: 500 907 2420
Fragments arranged by size:
2420 907 500
PciI A'CATG_T
Cuts at: 2283 2283
Size: 3827
PmeI GTTT'AAAC
Cuts at: 826 826
Size: 3827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 3795
PstI C_TGCA'G
Cuts at: 242 663 874 1559 242
Size: 421 211 685 2510
Fragments arranged by size:
2510 685 421 211
PvuI CG_AT'CG
Cuts at: 104 146 104
Size: 42 3785
PvuII CAG'CTG
Cuts at: 1252 1612 1252
Size: 360 3467
RsrII CG'GwC_CG
Cuts at: 2022 2022
Size: 3827
SacI G_AGCT'C
Cuts at: 841 841
Size: 3827
SalI G'TCGA_C
Cuts at: 864 864
Size: 3827
SapI GCTCTTCn'nnn_
Cuts at: 1850 2060 1850
Size: 210 3617
SbfI CC_TGCA'GG
Cuts at: 874 874
Size: 3827
ScaI AGT'ACT
Cuts at: 3344 3344
Size: 3827
SfcI C'TryA_G
Cuts at: 33 222 238 254 503 659 870 1555 2548
Size: 189 16 16 249 156 211 685 993
Cuts at: 2548 2739 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 249 211 191 189 156
16 16
SfoI GGC'GCC
Cuts at: 1259 1507 1259
Size: 248 3579
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3827
SmaI CCC'GGG
Cuts at: 849 849
Size: 3827
SmlI C'TyrA_G
Cuts at: 982 1897 2389 2651 982
Size: 915 492 262 2158
Fragments arranged by size:
2158 915 492 262
SnaBI TAC'GTA
Cuts at: 738 738
Size: 3827
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3827
SphI G_CATG'C
Cuts at: 880 1380 880
Size: 500 3327
StyI C'CwwG_G
Cuts at: 107 961 1315 107
Size: 854 354 2619
Fragments arranged by size:
2619 854 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1410 1543 2002 2211 1410
Size: 133 459 209 3026
Fragments arranged by size:
3026 459 209 133
TatI w'GTAC_w
Cuts at: 303 3342 303
Size: 3039 788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 3795
Tth111I GACn'n_nGTC
Cuts at: 1624 1624
Size: 3827
XbaI T'CTAG_A
Cuts at: 858 858
Size: 3827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1322 1322
Size: 3827
XmaI C'CCGG_G
Cuts at: 847 847
Size: 3827
XmnI GAAnn'nnTTC
Cuts at: 550 550
Size: 3827
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BsiWI BsmBI BssHII BstEII BstXI
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI ZraI