Restriction map for
pF1KB7438
(Circular) (Six-base) MAPSORT of: pF1KB7438.seq Check: 7925 from: 1 to: 4052
pF1KB7438 4052 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 278 278
Size: 4052
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AleI CACnn'nnGTG
Cuts at: 688 688
Size: 4052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 278 1054 278
Size: 776 3276
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaI G_GGCC'C
Cuts at: 623 623
Size: 4052
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 139 1056 3405 3661 139
Size: 917 2349 256 530
Fragments arranged by size:
2349 917 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 1068 1482 1730 1765 1068
Size: 414 248 35 3355
Fragments arranged by size:
3355 414 248 35
BanII G_rGCy'C
Cuts at: 623 1066 2096 623
Size: 443 1030 2579
Fragments arranged by size:
2579 1030 443
BbeI G_GCGC'C
Cuts at: 1486 1734 1486
Size: 248 3804
BbsI GAAGACnn'nnnn_
Cuts at: 810 1000 810
Size: 190 3862
BbvCI CC'TCA_GC
Cuts at: 1012 1012
Size: 4052
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 776 1572 776
Size: 796 3256
BglII A'GATC_T
Cuts at: 410 1567 3604 410
Size: 1157 2037 858
Fragments arranged by size:
2037 1157 858
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 623 1677 1770 2826 623
Size: 1054 93 1056 1849
Fragments arranged by size:
1849 1056 1054 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
Bpu10I CC'TnA_GC
Cuts at: 328 1012 328
Size: 684 3368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 2107 2599 2897 1228
Size: 879 492 298 2383
Fragments arranged by size:
2383 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 1483 1731 2433 3929 1483
Size: 248 702 1496 1606
Fragments arranged by size:
1606 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 524 1477 2235 2812 3308 524
Size: 953 758 577 496 1268
Fragments arranged by size:
1268 953 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1066 1844 2034 2826 1066
Size: 778 190 792 2292
Fragments arranged by size:
2292 792 778 190
BsmI GAATG_Cn'
Cuts at: 1031 1031
Size: 4052
Bsp1286I G_dGCh'C
Cuts at: 623 1066 1677 1770 1844 2034 2096 2826 623
Size: 443 611 93 74 190 62 730 1849
Fragments arranged by size:
1849 730 611 443 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 515 3228 515
Size: 2713 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 576 1102 1316 1618 1999 278
Size: 298 526 214 302 381 2331
Fragments arranged by size:
2331 526 381 302 298 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 212 1036 1964 212
Size: 824 928 2300
Fragments arranged by size:
2300 928 824
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 630 630
Size: 4052
BstYI r'GATC_y
Cuts at: 1 410 1077 1544 1567 1902 2148 3160 3246
Size: 409 667 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 667 467 449 409 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1295
Size: 4052
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 1637 1811 2202 2229 3574 1637
Size: 174 391 27 1345 2115
Fragments arranged by size:
2115 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 193 2075 2285 193
Size: 1882 210 1960
Fragments arranged by size:
1960 1882 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 370 471 370
Size: 101 3951
EcoO109I rG'GnC_Cy
Cuts at: 1191 1191
Size: 4052
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 1486 1734 2756 3735 1486
Size: 248 1022 979 1803
Fragments arranged by size:
1803 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1894 1924 1926 1956 1894
Size: 30 2 30 3990
Fragments arranged by size:
3990 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
KasI G'GCGC_C
Cuts at: 1482 1730 1482
Size: 248 3804
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2723 2907 2723
Size: 184 3868
MscI TGG'CCA
Cuts at: 1813 1813
Size: 4052
MslI CAynn'nnrTG
Cuts at: 688 2041 2168 688
Size: 1353 127 2572
Fragments arranged by size:
2572 1353 127
MspA1I CmG'CkG
Cuts at: 606 1163 1477 1837 2850 3095 606
Size: 557 314 360 1013 245 1563
Fragments arranged by size:
1563 1013 557 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 1483 1731 1483
Size: 248 3804
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 1105 1605 2512 1105
Size: 500 907 2645
Fragments arranged by size:
2645 907 500
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PshAI GACnn'nnGTC
Cuts at: 847 847
Size: 4052
PspOMI G'GGCC_C
Cuts at: 619 619
Size: 4052
PstI C_TGCA'G
Cuts at: 179 901 1099 1784 179
Size: 722 198 685 2447
Fragments arranged by size:
2447 722 685 198
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 606 1477 1837 606
Size: 871 360 2821
Fragments arranged by size:
2821 871 360
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 175 367 897 1095 1780 2773 2964 33
Size: 142 192 530 198 685 993 191 1121
Fragments arranged by size:
1121 993 685 530 198 192 191 142
SfoI GGC'GCC
Cuts at: 1484 1732 1484
Size: 248 3804
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 1207 2122 2614 2876 1207
Size: 915 492 262 2383
Fragments arranged by size:
2383 915 492 262
SpeI A'CTAG_T
Cuts at: 67 594 67
Size: 527 3525
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StuI AGG'CCT
Cuts at: 891 891
Size: 4052
StyI C'CwwG_G
Cuts at: 1186 1540 1186
Size: 354 3698
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1635 1768 2227 2436 1635
Size: 133 459 209 3251
Fragments arranged by size:
3251 459 209 133
TatI w'GTAC_w
Cuts at: 3567 3567
Size: 4052
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1547 1547
Size: 4052
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
XmnI GAAnn'nnTTC
Cuts at: 143 143
Size: 4052
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI BseRI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI