Restriction map for
pF1KB7439
(Circular) (Six-base) MAPSORT of: pF1KB7439.seq Check: 9685 from: 1 to: 3554
pF1KB7439 3554 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 141 141
Size: 3554
AccI GT'mk_AC
Cuts at: 592 592
Size: 3554
Acc65I G'GTAC_C
Cuts at: 570 570
Size: 3554
AclI AA'CG_TT
Cuts at: 3394 3394
Size: 3554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 1379 1811 2558 2826 295
Size: 1084 432 747 268 1023
Fragments arranged by size:
1084 1023 747 432 268
AflIII A'CryG_T
Cuts at: 906 2010 2987 906
Size: 1104 977 1473
Fragments arranged by size:
1473 1104 977
AgeI A'CCGG_T
Cuts at: 1942 1942
Size: 3554
AlwNI CAG_nnn'CTG
Cuts at: 859 2426 859
Size: 1567 1987
ApaLI G'TGCA_C
Cuts at: 2324 2324
Size: 3554
ApoI r'AATT_y
Cuts at: 208 329 558 2907 3163 208
Size: 121 229 2349 256 599
Fragments arranged by size:
2349 599 256 229 121
AseI AT'TA_AT
Cuts at: 20 20
Size: 3554
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3554
AvaI C'yCGr_G
Cuts at: 574 574
Size: 3554
BamHI G'GATC_C
Cuts at: 579 579
Size: 3554
BanI G'GyrC_C
Cuts at: 570 984 1232 1267 570
Size: 414 248 35 2857
Fragments arranged by size:
2857 414 248 35
BanII G_rGCy'C
Cuts at: 568 1598 568
Size: 1030 2524
BbeI G_GCGC'C
Cuts at: 988 1236 988
Size: 248 3306
BciVI GTATCCnnnnn_n'
Cuts at: 1452 2213 3554 1452
Size: 761 1341 1452
Fragments arranged by size:
1452 1341 761
BclI T'GATC_A
Cuts at: 1074 1074
Size: 3554
BglII A'GATC_T
Cuts at: 1069 3106 1069
Size: 2037 1517
BlpI GC'TnA_GC
Cuts at: 172 666 172
Size: 494 3060
Bme1580I G_kGCm'C
Cuts at: 1179 1272 2328 1179
Size: 93 1056 2405
Fragments arranged by size:
2405 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 766 847 1182 3326 766
Size: 81 335 2144 994
Fragments arranged by size:
2144 994 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1921 1921
Size: 3554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 429
Size: 3554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 730 1609 2101 2399 281
Size: 449 879 492 298 1436
Fragments arranged by size:
1436 879 492 449 298
BsaI GGTCTCn'nnnn_
Cuts at: 3258 3258
Size: 3554
BsaAI yAC'GTr
Cuts at: 907 907
Size: 3554
BsaBI GATnn'nnATC
Cuts at: 6 1068 3105 6
Size: 1062 2037 455
Fragments arranged by size:
2037 1062 455
BsaHI Gr'CG_yC
Cuts at: 368 985 1233 1935 3431 368
Size: 617 248 702 1496 491
Fragments arranged by size:
1496 702 617 491 248
BsaWI w'CCGG_w
Cuts at: 743 1264 1942 2216 2363 2774 743
Size: 521 678 274 147 411 1523
Fragments arranged by size:
1523 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 326 326
Size: 3554
BseYI C'CCAG_C
Cuts at: 350 376 979 1737 2314 2810 350
Size: 26 603 758 577 496 1094
Fragments arranged by size:
1094 758 603 577 496 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 368 342
Size: 26 3528
BsiEI CG_ry'CG
Cuts at: 104 1096 1142 2350 104
Size: 992 46 1208 1308
Fragments arranged by size:
1308 1208 992 46
BsiHKAI G_wGCw'C
Cuts at: 568 1346 1536 2328 568
Size: 778 190 792 1794
Fragments arranged by size:
1794 792 778 190
BsmI GAATG_Cn'
Cuts at: 503 503
Size: 3554
Bsp1286I G_dGCh'C
Cuts at: 568 1179 1272 1346 1536 1598 2328 568
Size: 611 93 74 190 62 730 1794
Fragments arranged by size:
1794 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2774 2774
Size: 3554
BspHI T'CATG_A
Cuts at: 2730 2730
Size: 3554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 141 604 818 1120 1501 141
Size: 463 214 302 381 2194
Fragments arranged by size:
2194 463 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1846 1900 3384 3549 50
Size: 1796 54 1484 165 55
Fragments arranged by size:
1796 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1466 1466
Size: 3554
BsrFI r'CCGG_y
Cuts at: 1552 1733 1942 1552
Size: 181 209 3164
Fragments arranged by size:
3164 209 181
BssSI C'ACGA_G
Cuts at: 1825 2183 1825
Size: 358 3196
BstAPI GCAn_nnn'nTGC
Cuts at: 391 931 391
Size: 540 3014
BstBI TT'CG_AA
Cuts at: 1915 1915
Size: 3554
BstYI r'GATC_y
Cuts at: 1 278 466 486 579 1046 1069 1404 1650
Size: 277 188 20 93 467 23 335 246
Cuts at: 1650 2662 2748 2760 3106 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 277 246 188
93 86 23 20 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1504 1685 1848 1504
Size: 181 163 3210
Fragments arranged by size:
3210 181 163
BtsI GCAGTG_nn'
Cuts at: 263 327 359 385 488 797 263
Size: 64 32 26 103 309 3020
Fragments arranged by size:
3020 309 103 64 32 26
DraI TTT'AAA
Cuts at: 553 553
Size: 3554
DraIII CAC_nnn'GTG
Cuts at: 391 527 391
Size: 136 3418
DrdI GACnn_nn'nnGTC
Cuts at: 1260 2118 1260
Size: 858 2696
EaeI y'GGCC_r
Cuts at: 1139 1313 1704 1731 3076 1139
Size: 174 391 27 1345 1617
Fragments arranged by size:
1617 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1139 1139
Size: 3554
EarI CTCTTCn'nnn_
Cuts at: 227 245 1577 1787 227
Size: 18 1332 210 1994
Fragments arranged by size:
1994 1332 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1762 2072 2218 3364 1762
Size: 310 146 1146 1952
Fragments arranged by size:
1952 1146 310 146
EcoICRI GAG'CTC
Cuts at: 566 566
Size: 3554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 429 1379 1811 2558 2826 295
Size: 134 950 432 747 268 1023
Fragments arranged by size:
1023 950 747 432 268 134
EcoNI CCTnn'n_nnAGG
Cuts at: 289 289
Size: 3554
EcoO109I rG'GnC_Cy
Cuts at: 693 693
Size: 3554
EcoRI G'AATT_C
Cuts at: 208 558 208
Size: 350 3204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 187 1573 187
Size: 1386 2168
FspI TGC'GCA
Cuts at: 341 1335 341
Size: 994 2560
HaeII r_GCGC'y
Cuts at: 988 1236 2258 3237 988
Size: 248 1022 979 1305
Fragments arranged by size:
1305 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 1396 1426 1428 1458 478
Size: 32 886 30 2 30 2574
Fragments arranged by size:
2574 886 32 30 30 2
HincII GTy'rAC
Cuts at: 593 593
Size: 3554
KasI G'GCGC_C
Cuts at: 984 1232 984
Size: 248 3306
KpnI G_GTAC'C
Cuts at: 574 574
Size: 3554
MluI A'CGCG_T
Cuts at: 2987 2987
Size: 3554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2225 2409 2225
Size: 184 3370
MscI TGG'CCA
Cuts at: 1315 1315
Size: 3554
MslI CAynn'nnrTG
Cuts at: 112 1543 1670 112
Size: 1431 127 1996
Fragments arranged by size:
1996 1431 127
MspA1I CmG'CkG
Cuts at: 337 665 979 1339 2352 2597 337
Size: 328 314 360 1013 245 1294
Fragments arranged by size:
1294 1013 360 328 314 245
NaeI GCC'GGC
Cuts at: 1735 1735
Size: 3554
NarI GG'CG_CC
Cuts at: 985 1233 985
Size: 248 3306
NgoMIV G'CCGG_C
Cuts at: 1733 1733
Size: 3554
NspI r_CATG'y
Cuts at: 607 1107 2014 607
Size: 500 907 2147
Fragments arranged by size:
2147 907 500
PciI A'CATG_T
Cuts at: 2010 2010
Size: 3554
PmeI GTTT'AAAC
Cuts at: 553 553
Size: 3554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2693 2725 2693
Size: 32 3522
PstI C_TGCA'G
Cuts at: 434 601 1286 434
Size: 167 685 2702
Fragments arranged by size:
2702 685 167
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3554
PvuII CAG'CTG
Cuts at: 337 979 1339 337
Size: 642 360 2552
Fragments arranged by size:
2552 642 360
RsrII CG'GwC_CG
Cuts at: 1749 1749
Size: 3554
SacI G_AGCT'C
Cuts at: 568 568
Size: 3554
SalI G'TCGA_C
Cuts at: 591 591
Size: 3554
SapI GCTCTTCn'nnn_
Cuts at: 1577 1787 1577
Size: 210 3344
SbfI CC_TGCA'GG
Cuts at: 434 601 434
Size: 167 3387
ScaI AGT'ACT
Cuts at: 3071 3071
Size: 3554
SfcI C'TryA_G
Cuts at: 33 430 597 1282 2275 2466 33
Size: 397 167 685 993 191 1121
Fragments arranged by size:
1121 993 685 397 191 167
SfoI GGC'GCC
Cuts at: 986 1234 986
Size: 248 3306
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3554
SmaI CCC'GGG
Cuts at: 576 576
Size: 3554
SmlI C'TyrA_G
Cuts at: 296 709 1624 2116 2378 296
Size: 413 915 492 262 1472
Fragments arranged by size:
1472 915 492 413 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3554
SphI G_CATG'C
Cuts at: 607 1107 607
Size: 500 3054
StyI C'CwwG_G
Cuts at: 688 1042 688
Size: 354 3200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1137 1270 1729 1938 1137
Size: 133 459 209 2753
Fragments arranged by size:
2753 459 209 133
TatI w'GTAC_w
Cuts at: 3069 3069
Size: 3554
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 263 295 3071 3103 263
Size: 32 2776 32 714
Fragments arranged by size:
2776 714 32 32
Tth111I GACn'n_nGTC
Cuts at: 1351 1351
Size: 3554
XbaI T'CTAG_A
Cuts at: 585 585
Size: 3554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1049 1049
Size: 3554
XmaI C'CCGG_G
Cuts at: 574 574
Size: 3554
XmnI GAAnn'nnTTC
Cuts at: 208 208
Size: 3554
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI