Restriction map for
pF1KB7440
(Circular) (Six-base) MAPSORT of: pF1KB7440.seq Check: 9656 from: 1 to: 3569
pF1KB7440 3569 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 607 607
Size: 3569
Acc65I G'GTAC_C
Cuts at: 585 585
Size: 3569
AclI AA'CG_TT
Cuts at: 3409 3409
Size: 3569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1394 1826 2573 2841 439
Size: 955 432 747 268 1167
Fragments arranged by size:
1167 955 747 432 268
AflIII A'CryG_T
Cuts at: 476 921 2025 3002 476
Size: 445 1104 977 1043
Fragments arranged by size:
1104 1043 977 445
AgeI A'CCGG_T
Cuts at: 1957 1957
Size: 3569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 524 524
Size: 3569
AlwNI CAG_nnn'CTG
Cuts at: 874 2441 874
Size: 1567 2002
ApaLI G'TGCA_C
Cuts at: 2339 2339
Size: 3569
ApoI r'AATT_y
Cuts at: 573 2922 3178 573
Size: 2349 256 964
Fragments arranged by size:
2349 964 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3569
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3569
AvaI C'yCGr_G
Cuts at: 254 589 254
Size: 335 3234
BamHI G'GATC_C
Cuts at: 594 594
Size: 3569
BanI G'GyrC_C
Cuts at: 250 585 999 1247 1282 250
Size: 335 414 248 35 2537
Fragments arranged by size:
2537 414 335 248 35
BanII G_rGCy'C
Cuts at: 497 583 1613 497
Size: 86 1030 2453
Fragments arranged by size:
2453 1030 86
BbeI G_GCGC'C
Cuts at: 254 1003 1251 254
Size: 749 248 2572
Fragments arranged by size:
2572 749 248
BciVI GTATCCnnnnn_n'
Cuts at: 1467 2228 3569 1467
Size: 761 1341 1467
Fragments arranged by size:
1467 1341 761
BclI T'GATC_A
Cuts at: 1089 1089
Size: 3569
BglII A'GATC_T
Cuts at: 1084 3121 1084
Size: 2037 1532
BlpI GC'TnA_GC
Cuts at: 681 681
Size: 3569
Bme1580I G_kGCm'C
Cuts at: 549 1194 1287 2343 549
Size: 645 93 1056 1775
Fragments arranged by size:
1775 1056 645 93
BmrI ACTGGGnnnn_n'
Cuts at: 781 862 1197 3341 781
Size: 81 335 2144 1009
Fragments arranged by size:
2144 1009 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1936 1936
Size: 3569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 3569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 745 1624 2116 2414 172
Size: 573 879 492 298 1327
Fragments arranged by size:
1327 879 573 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3273 3273
Size: 3569
BsaAI yAC'GTr
Cuts at: 550 922 550
Size: 372 3197
BsaBI GATnn'nnATC
Cuts at: 6 1083 3120 6
Size: 1077 2037 455
Fragments arranged by size:
2037 1077 455
BsaHI Gr'CG_yC
Cuts at: 251 1000 1248 1950 3446 251
Size: 749 248 702 1496 374
Fragments arranged by size:
1496 749 702 374 248
BsaWI w'CCGG_w
Cuts at: 758 1279 1957 2231 2378 2789 758
Size: 521 678 274 147 411 1538
Fragments arranged by size:
1538 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 382 412 382
Size: 30 3539
BseYI C'CCAG_C
Cuts at: 259 276 994 1752 2329 2825 259
Size: 17 718 758 577 496 1003
Fragments arranged by size:
1003 758 718 577 496 17
BsiEI CG_ry'CG
Cuts at: 104 1111 1157 2365 104
Size: 1007 46 1208 1308
Fragments arranged by size:
1308 1208 1007 46
BsiHKAI G_wGCw'C
Cuts at: 497 583 1361 1551 2343 497
Size: 86 778 190 792 1723
Fragments arranged by size:
1723 792 778 190 86
BsiWI C'GTAC_G
Cuts at: 176 176
Size: 3569
Bsp1286I G_dGCh'C
Cuts at: 497 549 583 1194 1287 1361 1551 1613 2343
Size: 52 34 611 93 74 190 62 730
Cuts at: 2343 497
Size: 1723
Fragments arranged by size:
1723 730 611 190 93 74 62 52
34
BspEI T'CCGG_A
Cuts at: 2789 2789
Size: 3569
BspHI T'CATG_A
Cuts at: 2745 2745
Size: 3569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 619 833 1135 1516 619
Size: 214 302 381 2672
Fragments arranged by size:
2672 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1861 1915 3399 3564 50
Size: 1811 54 1484 165 55
Fragments arranged by size:
1811 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1481 1481
Size: 3569
BsrFI r'CCGG_y
Cuts at: 247 1567 1748 1957 247
Size: 1320 181 209 1859
Fragments arranged by size:
1859 1320 209 181
BssSI C'ACGA_G
Cuts at: 1840 2198 1840
Size: 358 3211
BstAPI GCAn_nnn'nTGC
Cuts at: 946 946
Size: 3569
BstBI TT'CG_AA
Cuts at: 1930 1930
Size: 3569
BstXI CCAn_nnnn'nTGG
Cuts at: 542 542
Size: 3569
BstYI r'GATC_y
Cuts at: 1 594 1061 1084 1419 1665 2677 2763 2775
Size: 593 467 23 335 246 1012 86 12
Cuts at: 2775 3121 1
Size: 346 449
Fragments arranged by size:
1012 593 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 305 107
Size: 198 3371
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1519 1700 1863 1519
Size: 181 163 3225
Fragments arranged by size:
3225 181 163
BtsI GCAGTG_nn'
Cuts at: 812 812
Size: 3569
DraI TTT'AAA
Cuts at: 568 568
Size: 3569
DrdI GACnn_nn'nnGTC
Cuts at: 1275 2133 1275
Size: 858 2711
EaeI y'GGCC_r
Cuts at: 245 428 1154 1328 1719 1746 3091 245
Size: 183 726 174 391 27 1345 723
Fragments arranged by size:
1345 726 723 391 183 174 27
EagI C'GGCC_G
Cuts at: 1154 1154
Size: 3569
EarI CTCTTCn'nnn_
Cuts at: 485 1592 1802 485
Size: 1107 210 2252
Fragments arranged by size:
2252 1107 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 173 208 373 1777 2087 2233 3379 173
Size: 35 165 1404 310 146 1146 363
Fragments arranged by size:
1404 1146 363 310 165 146 35
EcoICRI GAG'CTC
Cuts at: 495 581 495
Size: 86 3483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 439 1394 1826 2573 2841 373
Size: 66 955 432 747 268 1101
Fragments arranged by size:
1101 955 747 432 268 66
EcoNI CCTnn'n_nnAGG
Cuts at: 186 186
Size: 3569
EcoO109I rG'GnC_Cy
Cuts at: 348 708 348
Size: 360 3209
EcoRI G'AATT_C
Cuts at: 573 573
Size: 3569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1588 1588
Size: 3569
FspI TGC'GCA
Cuts at: 1350 1350
Size: 3569
HaeII r_GCGC'y
Cuts at: 254 320 1003 1251 2273 3252 254
Size: 66 683 248 1022 979 571
Fragments arranged by size:
1022 979 683 571 248 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1411 1441 1443 1473 1411
Size: 30 2 30 3507
Fragments arranged by size:
3507 30 30 2
HincII GTy'rAC
Cuts at: 173 608 173
Size: 435 3134
KasI G'GCGC_C
Cuts at: 250 999 1247 250
Size: 749 248 2572
Fragments arranged by size:
2572 749 248
KpnI G_GTAC'C
Cuts at: 589 589
Size: 3569
MluI A'CGCG_T
Cuts at: 476 3002 476
Size: 2526 1043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2240 2424 2240
Size: 184 3385
MscI TGG'CCA
Cuts at: 1330 1330
Size: 3569
MslI CAynn'nnrTG
Cuts at: 506 1558 1685 506
Size: 1052 127 2390
Fragments arranged by size:
2390 1052 127
MspA1I CmG'CkG
Cuts at: 134 211 293 345 450 680 994 1354 2367
Size: 77 82 52 105 230 314 360 1013
Cuts at: 2367 2612 134
Size: 245 1091
Fragments arranged by size:
1091 1013 360 314 245 230 105 82
77 52
NaeI GCC'GGC
Cuts at: 249 1750 249
Size: 1501 2068
NarI GG'CG_CC
Cuts at: 251 1000 1248 251
Size: 749 248 2572
Fragments arranged by size:
2572 749 248
NcoI C'CATG_G
Cuts at: 107 305 107
Size: 198 3371
NgoMIV G'CCGG_C
Cuts at: 247 1748 247
Size: 1501 2068
NspI r_CATG'y
Cuts at: 367 622 1122 2029 367
Size: 255 500 907 1907
Fragments arranged by size:
1907 907 500 255
PciI A'CATG_T
Cuts at: 2025 2025
Size: 3569
PmeI GTTT'AAAC
Cuts at: 568 568
Size: 3569
PmlI CAC'GTG
Cuts at: 550 550
Size: 3569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 2740 2708
Size: 32 3537
PpuMI rG'GwC_Cy
Cuts at: 348 348
Size: 3569
PstI C_TGCA'G
Cuts at: 344 616 1301 344
Size: 272 685 2612
Fragments arranged by size:
2612 685 272
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3569
PvuII CAG'CTG
Cuts at: 293 994 1354 293
Size: 701 360 2508
Fragments arranged by size:
2508 701 360
RsrII CG'GwC_CG
Cuts at: 1764 1764
Size: 3569
SacI G_AGCT'C
Cuts at: 497 583 497
Size: 86 3483
SalI G'TCGA_C
Cuts at: 606 606
Size: 3569
SapI GCTCTTCn'nnn_
Cuts at: 485 1592 1802 485
Size: 1107 210 2252
Fragments arranged by size:
2252 1107 210
SbfI CC_TGCA'GG
Cuts at: 616 616
Size: 3569
ScaI AGT'ACT
Cuts at: 165 3086 165
Size: 2921 648
SfcI C'TryA_G
Cuts at: 33 340 402 612 1297 2290 2481 33
Size: 307 62 210 685 993 191 1121
Fragments arranged by size:
1121 993 685 307 210 191 62
SfoI GGC'GCC
Cuts at: 252 1001 1249 252
Size: 749 248 2572
Fragments arranged by size:
2572 749 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3569
SmaI CCC'GGG
Cuts at: 591 591
Size: 3569
SmlI C'TyrA_G
Cuts at: 151 724 1639 2131 2393 151
Size: 573 915 492 262 1327
Fragments arranged by size:
1327 915 573 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3569
SphI G_CATG'C
Cuts at: 622 1122 622
Size: 500 3069
StyI C'CwwG_G
Cuts at: 107 305 703 1057 107
Size: 198 398 354 2619
Fragments arranged by size:
2619 398 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1152 1285 1744 1953 1152
Size: 133 459 209 2768
Fragments arranged by size:
2768 459 209 133
TatI w'GTAC_w
Cuts at: 163 3084 163
Size: 2921 648
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 3537
Tth111I GACn'n_nGTC
Cuts at: 1366 1366
Size: 3569
XbaI T'CTAG_A
Cuts at: 600 600
Size: 3569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1064 1064
Size: 3569
XmaI C'CCGG_G
Cuts at: 589 589
Size: 3569
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsgI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI