Restriction map for
pF1KB7441
(Circular) (Six-base) MAPSORT of: pF1KB7441.seq Check: 9190 from: 1 to: 3758
pF1KB7441 3758 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 707 707
Size: 3758
AccI GT'mk_AC
Cuts at: 281 796 281
Size: 515 3243
Acc65I G'GTAC_C
Cuts at: 774 774
Size: 3758
AclI AA'CG_TT
Cuts at: 3598 3598
Size: 3758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1583 2015 2762 3030 1583
Size: 432 747 268 2311
Fragments arranged by size:
2311 747 432 268
AflIII A'CryG_T
Cuts at: 162 1110 2214 3191 162
Size: 948 1104 977 729
Fragments arranged by size:
1104 977 948 729
AgeI A'CCGG_T
Cuts at: 2146 2146
Size: 3758
AleI CACnn'nnGTG
Cuts at: 163 163
Size: 3758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 155 155
Size: 3758
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 472 504 472
Size: 32 3726
AlwNI CAG_nnn'CTG
Cuts at: 1063 2630 1063
Size: 1567 2191
ApaLI G'TGCA_C
Cuts at: 111 166 2528 111
Size: 55 2362 1341
Fragments arranged by size:
2362 1341 55
ApoI r'AATT_y
Cuts at: 762 3111 3367 762
Size: 2349 256 1153
Fragments arranged by size:
2349 1153 256
AseI AT'TA_AT
Cuts at: 20 722 20
Size: 702 3056
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
AvaI C'yCGr_G
Cuts at: 778 778
Size: 3758
BamHI G'GATC_C
Cuts at: 783 783
Size: 3758
BanI G'GyrC_C
Cuts at: 774 1188 1436 1471 774
Size: 414 248 35 3061
Fragments arranged by size:
3061 414 248 35
BanII G_rGCy'C
Cuts at: 208 701 772 1802 208
Size: 493 71 1030 2164
Fragments arranged by size:
2164 1030 493 71
BbeI G_GCGC'C
Cuts at: 1192 1440 1192
Size: 248 3510
BciVI GTATCCnnnnn_n'
Cuts at: 1656 2417 3758 1656
Size: 761 1341 1656
Fragments arranged by size:
1656 1341 761
BclI T'GATC_A
Cuts at: 654 1278 654
Size: 624 3134
BglII A'GATC_T
Cuts at: 136 1273 3310 136
Size: 1137 2037 584
Fragments arranged by size:
2037 1137 584
BlpI GC'TnA_GC
Cuts at: 870 870
Size: 3758
Bme1580I G_kGCm'C
Cuts at: 115 170 1383 1476 2532 115
Size: 55 1213 93 1056 1341
Fragments arranged by size:
1341 1213 1056 93 55
BmrI ACTGGGnnnn_n'
Cuts at: 970 1051 1386 3530 970
Size: 81 335 2144 1198
Fragments arranged by size:
2144 1198 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 3758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 3758
Bpu10I CC'TnA_GC
Cuts at: 371 371
Size: 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 543 934 1813 2305 2603 543
Size: 391 879 492 298 1698
Fragments arranged by size:
1698 879 492 391 298
BsaI GGTCTCn'nnnn_
Cuts at: 298 3462 298
Size: 3164 594
BsaAI yAC'GTr
Cuts at: 477 1111 477
Size: 634 3124
BsaBI GATnn'nnATC
Cuts at: 6 1272 3309 6
Size: 1266 2037 455
Fragments arranged by size:
2037 1266 455
BsaHI Gr'CG_yC
Cuts at: 704 1189 1437 2139 3635 704
Size: 485 248 702 1496 827
Fragments arranged by size:
1496 827 702 485 248
BsaWI w'CCGG_w
Cuts at: 947 1468 2146 2420 2567 2978 947
Size: 521 678 274 147 411 1727
Fragments arranged by size:
1727 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1183 1941 2518 3014 1183
Size: 758 577 496 1927
Fragments arranged by size:
1927 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1300 1346 2554 104
Size: 1196 46 1208 1308
Fragments arranged by size:
1308 1208 1196 46
BsiHKAI G_wGCw'C
Cuts at: 115 170 277 701 772 1550 1740 2532 115
Size: 55 107 424 71 778 190 792 1341
Fragments arranged by size:
1341 792 778 424 190 107 71 55
Bsp1286I G_dGCh'C
Cuts at: 115 170 208 277 701 772 1383 1476 1550
Size: 55 38 69 424 71 611 93 74
Cuts at: 1550 1740 1802 2532 115
Size: 190 62 730 1341
Fragments arranged by size:
1341 730 611 424 190 93 74 71
69 62 55 38
BspEI T'CCGG_A
Cuts at: 2978 2978
Size: 3758
BspHI T'CATG_A
Cuts at: 732 2934 732
Size: 2202 1556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 808 1022 1324 1705 808
Size: 214 302 381 2861
Fragments arranged by size:
2861 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2050 2104 3588 3753 50
Size: 2000 54 1484 165 55
Fragments arranged by size:
2000 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 539 586 1670 539
Size: 47 1084 2627
Fragments arranged by size:
2627 1084 47
BsrFI r'CCGG_y
Cuts at: 1756 1937 2146 1756
Size: 181 209 3368
Fragments arranged by size:
3368 209 181
BssSI C'ACGA_G
Cuts at: 2029 2387 2029
Size: 358 3400
BstAPI GCAn_nnn'nTGC
Cuts at: 1135 1135
Size: 3758
BstBI TT'CG_AA
Cuts at: 2119 2119
Size: 3758
BstXI CCAn_nnnn'nTGG
Cuts at: 200 200
Size: 3758
BstYI r'GATC_y
Cuts at: 1 136 783 1250 1273 1608 1854 2866 2952
Size: 135 647 467 23 335 246 1012 86
Cuts at: 2952 2964 3310 1
Size: 12 346 449
Fragments arranged by size:
1012 647 467 449 346 335 246 135
86 23 12
BtgI C'CryG_G
Cuts at: 214 288 214
Size: 74 3684
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1708 1889 2052 1708
Size: 181 163 3414
Fragments arranged by size:
3414 181 163
BtsI GCAGTG_nn'
Cuts at: 603 604 1001 603
Size: 1 397 3360
Fragments arranged by size:
3360 397 1
DraI TTT'AAA
Cuts at: 757 757
Size: 3758
DrdI GACnn_nn'nnGTC
Cuts at: 1464 2322 1464
Size: 858 2900
EaeI y'GGCC_r
Cuts at: 1343 1517 1908 1935 3280 1343
Size: 174 391 27 1345 1821
Fragments arranged by size:
1821 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1343 1343
Size: 3758
EarI CTCTTCn'nnn_
Cuts at: 329 1781 1991 329
Size: 1452 210 2096
Fragments arranged by size:
2096 1452 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1966 2276 2422 3568 1966
Size: 310 146 1146 2156
Fragments arranged by size:
2156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 699 770 699
Size: 71 3687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1583 2015 2762 3030 682
Size: 901 432 747 268 1410
Fragments arranged by size:
1410 901 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 897 897
Size: 3758
EcoRI G'AATT_C
Cuts at: 762 762
Size: 3758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 396 1777 396
Size: 1381 2377
FspI TGC'GCA
Cuts at: 1539 1539
Size: 3758
HaeII r_GCGC'y
Cuts at: 1192 1440 2462 3441 1192
Size: 248 1022 979 1509
Fragments arranged by size:
1509 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 585 617 1600 1630 1632 1662 585
Size: 32 983 30 2 30 2681
Fragments arranged by size:
2681 983 32 30 30 2
HincII GTy'rAC
Cuts at: 797 797
Size: 3758
KasI G'GCGC_C
Cuts at: 1188 1436 1188
Size: 248 3510
KpnI G_GTAC'C
Cuts at: 778 778
Size: 3758
MluI A'CGCG_T
Cuts at: 3191 3191
Size: 3758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2429 2613 2429
Size: 184 3574
MscI TGG'CCA
Cuts at: 1519 1519
Size: 3758
MslI CAynn'nnrTG
Cuts at: 163 179 185 319 1747 1874 163
Size: 16 6 134 1428 127 2047
Fragments arranged by size:
2047 1428 134 127 16 6
MspA1I CmG'CkG
Cuts at: 660 751 869 1183 1543 2556 2801 660
Size: 91 118 314 360 1013 245 1617
Fragments arranged by size:
1617 1013 360 314 245 118 91
NaeI GCC'GGC
Cuts at: 1939 1939
Size: 3758
NarI GG'CG_CC
Cuts at: 1189 1437 1189
Size: 248 3510
NgoMIV G'CCGG_C
Cuts at: 1937 1937
Size: 3758
NspI r_CATG'y
Cuts at: 166 324 564 811 1311 2218 166
Size: 158 240 247 500 907 1706
Fragments arranged by size:
1706 907 500 247 240 158
PasI CC'CwG_GG
Cuts at: 690 690
Size: 3758
PciI A'CATG_T
Cuts at: 162 2214 162
Size: 2052 1706
PmeI GTTT'AAAC
Cuts at: 757 757
Size: 3758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3726
PstI C_TGCA'G
Cuts at: 422 610 805 1490 422
Size: 188 195 685 2690
Fragments arranged by size:
2690 685 195 188
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3758
PvuII CAG'CTG
Cuts at: 660 751 1183 1543 660
Size: 91 432 360 2875
Fragments arranged by size:
2875 432 360 91
RsrII CG'GwC_CG
Cuts at: 1953 1953
Size: 3758
SacI G_AGCT'C
Cuts at: 701 772 701
Size: 71 3687
SalI G'TCGA_C
Cuts at: 795 795
Size: 3758
SapI GCTCTTCn'nnn_
Cuts at: 1781 1991 1781
Size: 210 3548
SbfI CC_TGCA'GG
Cuts at: 805 805
Size: 3758
ScaI AGT'ACT
Cuts at: 3275 3275
Size: 3758
SfcI C'TryA_G
Cuts at: 33 418 606 801 1486 2479 2670 33
Size: 385 188 195 685 993 191 1121
Fragments arranged by size:
1121 993 685 385 195 191 188
SfoI GGC'GCC
Cuts at: 1190 1438 1190
Size: 248 3510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
SmaI CCC'GGG
Cuts at: 780 780
Size: 3758
SmlI C'TyrA_G
Cuts at: 522 913 1828 2320 2582 522
Size: 391 915 492 262 1698
Fragments arranged by size:
1698 915 492 391 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3758
SphI G_CATG'C
Cuts at: 811 1311 811
Size: 500 3258
StyI C'CwwG_G
Cuts at: 892 1246 892
Size: 354 3404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1341 1474 1933 2142 1341
Size: 133 459 209 2957
Fragments arranged by size:
2957 459 209 133
TatI w'GTAC_w
Cuts at: 3273 3273
Size: 3758
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 3726
Tth111I GACn'n_nGTC
Cuts at: 1555 1555
Size: 3758
XbaI T'CTAG_A
Cuts at: 789 789
Size: 3758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 462 687 1253 462
Size: 225 566 2967
Fragments arranged by size:
2967 566 225
XmaI C'CCGG_G
Cuts at: 778 778
Size: 3758
ZraI GAC'GTC
Cuts at: 705 705
Size: 3758
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI