Restriction map for
pF1KB7442
(Circular) (Six-base) MAPSORT of: pF1KB7442.seq Check: 2357 from: 1 to: 4148
pF1KB7442 4148 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 639 639
Size: 4148
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 1164 1164
Size: 4148
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 1144 1973 2405 3152 3420 893
Size: 251 829 432 747 268 1621
Fragments arranged by size:
1621 829 747 432 268 251
AflII C'TTAA_G
Cuts at: 825 825
Size: 4148
AflIII A'CryG_T
Cuts at: 1500 2604 3581 1500
Size: 1104 977 2067
Fragments arranged by size:
2067 1104 977
AgeI A'CCGG_T
Cuts at: 2536 2536
Size: 4148
AhdI GACnn_n'nnGTC
Cuts at: 635 635
Size: 4148
AlwNI CAG_nnn'CTG
Cuts at: 916 1453 3020 916
Size: 537 1567 2044
Fragments arranged by size:
2044 1567 537
ApaLI G'TGCA_C
Cuts at: 367 2918 367
Size: 2551 1597
ApoI r'AATT_y
Cuts at: 1129 1152 3501 3757 1129
Size: 23 2349 256 1520
Fragments arranged by size:
2349 1520 256 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 1168 1168
Size: 4148
BamHI G'GATC_C
Cuts at: 1173 1173
Size: 4148
BanI G'GyrC_C
Cuts at: 1164 1578 1826 1861 1164
Size: 414 248 35 3451
Fragments arranged by size:
3451 414 248 35
BanII G_rGCy'C
Cuts at: 547 1006 1162 2192 547
Size: 459 156 1030 2503
Fragments arranged by size:
2503 1030 459 156
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BbsI GAAGACnn'nnnn_
Cuts at: 1042 1042
Size: 4148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4114
BciVI GTATCCnnnnn_n'
Cuts at: 2046 2807 4148 2046
Size: 761 1341 2046
Fragments arranged by size:
2046 1341 761
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4148
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 371 1773 1866 2922 371
Size: 1402 93 1056 1597
Fragments arranged by size:
1597 1402 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 381 1360 1441 1776 3920 381
Size: 979 81 335 2144 609
Fragments arranged by size:
2144 979 609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1136 2515 1136
Size: 1379 2769
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 502 222
Size: 280 3868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 2203 2695 2993 1324
Size: 879 492 298 2479
Fragments arranged by size:
2479 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 953 3852 953
Size: 2899 1249
BsaAI yAC'GTr
Cuts at: 1501 1501
Size: 4148
BsaBI GATnn'nnATC
Cuts at: 6 697 1662 3699 6
Size: 691 965 2037 455
Fragments arranged by size:
2037 965 691 455
BsaHI Gr'CG_yC
Cuts at: 636 1579 1827 2529 4025 636
Size: 943 248 702 1496 759
Fragments arranged by size:
1496 943 759 702 248
BsaWI w'CCGG_w
Cuts at: 1337 1858 2536 2810 2957 3368 1337
Size: 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 758 289
Size: 469 3679
BseYI C'CCAG_C
Cuts at: 229 457 665 762 916 1573 2331 2908 3404
Size: 228 208 97 154 657 758 577 496
Cuts at: 3404 229
Size: 973
Fragments arranged by size:
973 758 657 577 496 228 208 154
97
BsiEI CG_ry'CG
Cuts at: 104 1690 1736 2944 104
Size: 1586 46 1208 1308
Fragments arranged by size:
1586 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 311 371 1162 1940 2130 2922 311
Size: 60 791 778 190 792 1537
Fragments arranged by size:
1537 792 791 778 190 60
BsmI GAATG_Cn'
Cuts at: 559 559
Size: 4148
BsmBI CGTCTCn'nnnn_
Cuts at: 643 643
Size: 4148
Bsp1286I G_dGCh'C
Cuts at: 311 371 547 1006 1162 1773 1866 1940 2130
Size: 60 176 459 156 611 93 74 190
Cuts at: 2130 2192 2922 311
Size: 62 730 1537
Fragments arranged by size:
1537 730 611 459 190 176 156 93
74 62 60
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 3324 3324
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1198 1412 1714 2095 1198
Size: 214 302 381 3251
Fragments arranged by size:
3251 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2440 2494 3978 4143 50
Size: 2390 54 1484 165 55
Fragments arranged by size:
2390 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 351 2060 351
Size: 1709 2439
BsrFI r'CCGG_y
Cuts at: 2146 2327 2536 2146
Size: 181 209 3758
Fragments arranged by size:
3758 209 181
BsrGI T'GTAC_A
Cuts at: 398 398
Size: 4148
BssSI C'ACGA_G
Cuts at: 2419 2777 2419
Size: 358 3790
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstXI CCAn_nnnn'nTGG
Cuts at: 465 654 465
Size: 189 3959
BstYI r'GATC_y
Cuts at: 1 293 730 1173 1640 1663 1998 2244 3256
Size: 292 437 443 467 23 335 246 1012
Cuts at: 3256 3342 3354 3700 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 443 437 346 335 292
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2098 2279 2442 2098
Size: 181 163 3804
Fragments arranged by size:
3804 181 163
BtsI GCAGTG_nn'
Cuts at: 1069 1391 1069
Size: 322 3826
ClaI AT'CG_AT
Cuts at: 585 585
Size: 4148
DraI TTT'AAA
Cuts at: 134 1147 134
Size: 1013 3135
DrdI GACnn_nn'nnGTC
Cuts at: 1854 2712 1854
Size: 858 3290
EaeI y'GGCC_r
Cuts at: 467 1733 1907 2298 2325 3670 467
Size: 1266 174 391 27 1345 945
Fragments arranged by size:
1345 1266 945 391 174 27
EagI C'GGCC_G
Cuts at: 1733 1733
Size: 4148
EarI CTCTTCn'nnn_
Cuts at: 316 776 862 2171 2381 316
Size: 460 86 1309 210 2083
Fragments arranged by size:
2083 1309 460 210 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2356 2666 2812 3958 2356
Size: 310 146 1146 2546
Fragments arranged by size:
2546 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1160 1160
Size: 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 502 893 1144 1973 2405 3152 3420 222
Size: 280 391 251 829 432 747 268 950
Fragments arranged by size:
950 829 747 432 391 280 268 251
EcoO109I rG'GnC_Cy
Cuts at: 796 869 903 1287 796
Size: 73 34 384 3657
Fragments arranged by size:
3657 384 73 34
EcoRI G'AATT_C
Cuts at: 1152 1152
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 947 2167 947
Size: 1220 2928
FspI TGC'GCA
Cuts at: 1929 1929
Size: 4148
HaeII r_GCGC'y
Cuts at: 1582 1830 2852 3831 1582
Size: 248 1022 979 1899
Fragments arranged by size:
1899 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 138 170 621 653 1029 1061 1077 1109 1990
Size: 32 451 32 376 32 16 32 881
Cuts at: 1990 2020 2022 2052 138
Size: 30 2 30 2234
Fragments arranged by size:
2234 881 451 376 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 705 1187 705
Size: 482 3666
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 1168 1168
Size: 4148
MfeI C'AATT_G
Cuts at: 660 660
Size: 4148
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 643 2819 3003 643
Size: 2176 184 1788
Fragments arranged by size:
2176 1788 184
MscI TGG'CCA
Cuts at: 469 1909 469
Size: 1440 2708
MslI CAynn'nnrTG
Cuts at: 2137 2264 2137
Size: 127 4021
MspA1I CmG'CkG
Cuts at: 916 1259 1573 1933 2946 3191 916
Size: 343 314 360 1013 245 1873
Fragments arranged by size:
1873 1013 360 343 314 245
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 4148
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 4148
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4148
NspI r_CATG'y
Cuts at: 465 1201 1701 2608 465
Size: 736 500 907 2005
Fragments arranged by size:
2005 907 736 500
PasI CC'CwG_GG
Cuts at: 281 281
Size: 4148
PciI A'CATG_T
Cuts at: 2604 2604
Size: 4148
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PpuMI rG'GwC_Cy
Cuts at: 869 903 869
Size: 34 4114
PstI C_TGCA'G
Cuts at: 1195 1880 1195
Size: 685 3463
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 916 1573 1933 916
Size: 657 360 3131
Fragments arranged by size:
3131 657 360
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 1162 1162
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SanDI GG'GwC_CC
Cuts at: 903 903
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 316 2171 2381 316
Size: 1855 210 2083
Fragments arranged by size:
2083 1855 210
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 986 3665 986
Size: 2679 1469
SfcI C'TryA_G
Cuts at: 33 548 1191 1876 2869 3060 33
Size: 515 643 685 993 191 1121
Fragments arranged by size:
1121 993 685 643 515 191
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 1170 1170
Size: 4148
SmlI C'TyrA_G
Cuts at: 825 1303 2218 2710 2972 825
Size: 478 915 492 262 2001
Fragments arranged by size:
2001 915 492 478 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 465 1201 1701 465
Size: 736 500 2912
Fragments arranged by size:
2912 736 500
StyI C'CwwG_G
Cuts at: 539 1282 1636 539
Size: 743 354 3051
Fragments arranged by size:
3051 743 354
SwaI ATTT'AAAT
Cuts at: 134 134
Size: 4148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1731 1864 2323 2532 1731
Size: 133 459 209 3347
Fragments arranged by size:
3347 459 209 133
TatI w'GTAC_w
Cuts at: 398 984 3663 398
Size: 586 2679 883
Fragments arranged by size:
2679 883 586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4116
Tth111I GACn'n_nGTC
Cuts at: 1945 1945
Size: 4148
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1643 1643
Size: 4148
XmaI C'CCGG_G
Cuts at: 1168 1168
Size: 4148
XmnI GAAnn'nnTTC
Cuts at: 217 217
Size: 4148
ZraI GAC'GTC
Cuts at: 637 637
Size: 4148
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AleI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BssHII BstEII BstZ17I Bsu36I BtgI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI PacI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI XhoI