Restriction map for
pF1KB7443
(Circular) (Six-base) MAPSORT of: pF1KB7443.seq Check: 9347 from: 1 to: 3989
pF1KB7443 3989 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 377 485 958 377
Size: 108 473 3408
Fragments arranged by size:
3408 473 108
AatII G_ACGT'C
Cuts at: 770 770
Size: 3989
AccI GT'mk_AC
Cuts at: 1027 1027
Size: 3989
Acc65I G'GTAC_C
Cuts at: 1005 1005
Size: 3989
AclI AA'CG_TT
Cuts at: 3829 3829
Size: 3989
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1814 2246 2993 3261 445
Size: 1369 432 747 268 1173
Fragments arranged by size:
1369 1173 747 432 268
AflII C'TTAA_G
Cuts at: 138 138
Size: 3989
AflIII A'CryG_T
Cuts at: 1341 2445 3422 1341
Size: 1104 977 1908
Fragments arranged by size:
1908 1104 977
AgeI A'CCGG_T
Cuts at: 2377 2377
Size: 3989
AleI CACnn'nnGTG
Cuts at: 256 784 256
Size: 528 3461
AlwNI CAG_nnn'CTG
Cuts at: 741 1294 2861 741
Size: 553 1567 1869
Fragments arranged by size:
1869 1567 553
ApaLI G'TGCA_C
Cuts at: 2759 2759
Size: 3989
ApoI r'AATT_y
Cuts at: 993 3342 3598 993
Size: 2349 256 1384
Fragments arranged by size:
2349 1384 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3989
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
AvaI C'yCGr_G
Cuts at: 730 936 1009 730
Size: 206 73 3710
Fragments arranged by size:
3710 206 73
BamHI G'GATC_C
Cuts at: 1014 1014
Size: 3989
BanI G'GyrC_C
Cuts at: 473 499 1005 1419 1667 1702 473
Size: 26 506 414 248 35 2760
Fragments arranged by size:
2760 506 414 248 35 26
BanII G_rGCy'C
Cuts at: 126 326 1003 2033 126
Size: 200 677 1030 2082
Fragments arranged by size:
2082 1030 677 200
BbeI G_GCGC'C
Cuts at: 1423 1671 1423
Size: 248 3741
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 472 506 472
Size: 34 3955
BciVI GTATCCnnnnn_n'
Cuts at: 1887 2648 3989 1887
Size: 761 1341 1887
Fragments arranged by size:
1887 1341 761
BclI T'GATC_A
Cuts at: 1509 1509
Size: 3989
BglII A'GATC_T
Cuts at: 1504 3541 1504
Size: 2037 1952
BlpI GC'TnA_GC
Cuts at: 1101 1101
Size: 3989
Bme1580I G_kGCm'C
Cuts at: 1614 1707 2763 1614
Size: 93 1056 2840
Fragments arranged by size:
2840 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 821 1201 1282 1617 3761 821
Size: 380 81 335 2144 1049
Fragments arranged by size:
2144 1049 380 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 3989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 263
Size: 3989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 2044 2536 2834 1165
Size: 879 492 298 2320
Fragments arranged by size:
2320 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3693 3693
Size: 3989
BsaAI yAC'GTr
Cuts at: 1342 1342
Size: 3989
BsaBI GATnn'nnATC
Cuts at: 6 1503 3540 6
Size: 1497 2037 455
Fragments arranged by size:
2037 1497 455
BsaHI Gr'CG_yC
Cuts at: 767 1420 1668 2370 3866 767
Size: 653 248 702 1496 890
Fragments arranged by size:
1496 890 702 653 248
BsaWI w'CCGG_w
Cuts at: 1178 1699 2377 2651 2798 3209 1178
Size: 521 678 274 147 411 1958
Fragments arranged by size:
1958 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 406 1414 2172 2749 3245 406
Size: 1008 758 577 496 1150
Fragments arranged by size:
1150 1008 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 910 670
Size: 240 3749
BsiEI CG_ry'CG
Cuts at: 104 645 1531 1577 2785 104
Size: 541 886 46 1208 1308
Fragments arranged by size:
1308 1208 886 541 46
BsiHKAI G_wGCw'C
Cuts at: 1003 1781 1971 2763 1003
Size: 778 190 792 2229
Fragments arranged by size:
2229 792 778 190
BsiWI C'GTAC_G
Cuts at: 262 262
Size: 3989
BsmBI CGTCTCn'nnnn_
Cuts at: 459 459
Size: 3989
Bsp1286I G_dGCh'C
Cuts at: 126 326 1003 1614 1707 1781 1971 2033 2763
Size: 200 677 611 93 74 190 62 730
Cuts at: 2763 126
Size: 1352
Fragments arranged by size:
1352 730 677 611 200 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3209 3209
Size: 3989
BspHI T'CATG_A
Cuts at: 3165 3165
Size: 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 377 485 958 1039 1253 1555 1936 377
Size: 108 473 81 214 302 381 2430
Fragments arranged by size:
2430 473 381 302 214 108 81
BsrBI CCG'CTC
Cuts at: 50 347 717 2281 2335 3819 3984 50
Size: 297 370 1564 54 1484 165 55
Fragments arranged by size:
1564 1484 370 297 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1901 1901
Size: 3989
BsrFI r'CCGG_y
Cuts at: 222 563 1987 2168 2377 222
Size: 341 1424 181 209 1834
Fragments arranged by size:
1834 1424 341 209 181
BssSI C'ACGA_G
Cuts at: 2260 2618 2260
Size: 358 3631
BstAPI GCAn_nnn'nTGC
Cuts at: 1366 1366
Size: 3989
BstBI TT'CG_AA
Cuts at: 2350 2350
Size: 3989
BstYI r'GATC_y
Cuts at: 1 1014 1481 1504 1839 2085 3097 3183 3195
Size: 1013 467 23 335 246 1012 86 12
Cuts at: 3195 3541 1
Size: 346 449
Fragments arranged by size:
1013 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 289 495 674 980 289
Size: 206 179 306 3298
Fragments arranged by size:
3298 306 206 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1939 2120 2283 1939
Size: 181 163 3645
Fragments arranged by size:
3645 181 163
BtsI GCAGTG_nn'
Cuts at: 1232 1232
Size: 3989
ClaI AT'CG_AT
Cuts at: 197 197
Size: 3989
DraI TTT'AAA
Cuts at: 988 988
Size: 3989
DraIII CAC_nnn'GTG
Cuts at: 172 172
Size: 3989
DrdI GACnn_nn'nnGTC
Cuts at: 1695 2553 1695
Size: 858 3131
EaeI y'GGCC_r
Cuts at: 266 1574 1748 2139 2166 3511 266
Size: 1308 174 391 27 1345 744
Fragments arranged by size:
1345 1308 744 391 174 27
EagI C'GGCC_G
Cuts at: 1574 1574
Size: 3989
EarI CTCTTCn'nnn_
Cuts at: 421 2012 2222 421
Size: 1591 210 2188
Fragments arranged by size:
2188 1591 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2197 2507 2653 3799 2197
Size: 310 146 1146 2387
Fragments arranged by size:
2387 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1001 1001
Size: 3989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 445 1814 2246 2993 3261 263
Size: 182 1369 432 747 268 991
Fragments arranged by size:
1369 991 747 432 268 182
EcoO109I rG'GnC_Cy
Cuts at: 594 612 696 1128 594
Size: 18 84 432 3455
Fragments arranged by size:
3455 432 84 18
EcoRI G'AATT_C
Cuts at: 993 993
Size: 3989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2008 2008
Size: 3989
FspI TGC'GCA
Cuts at: 1770 1770
Size: 3989
HaeII r_GCGC'y
Cuts at: 684 1423 1671 2693 3672 684
Size: 739 248 1022 979 1001
Fragments arranged by size:
1022 1001 979 739 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 170 202 804 836 1831 1861 1863 1893 170
Size: 32 602 32 995 30 2 30 2266
Fragments arranged by size:
2266 995 602 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1028 1028
Size: 3989
KasI G'GCGC_C
Cuts at: 1419 1667 1419
Size: 248 3741
KpnI G_GTAC'C
Cuts at: 1009 1009
Size: 3989
MluI A'CGCG_T
Cuts at: 3422 3422
Size: 3989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 680 2660 2844 680
Size: 1980 184 1825
Fragments arranged by size:
1980 1825 184
MscI TGG'CCA
Cuts at: 1750 1750
Size: 3989
MslI CAynn'nnrTG
Cuts at: 256 523 784 827 1978 2105 256
Size: 267 261 43 1151 127 2140
Fragments arranged by size:
2140 1151 267 261 127 43
MspA1I CmG'CkG
Cuts at: 497 666 702 924 1100 1414 1774 2787 3032
Size: 169 36 222 176 314 360 1013 245
Cuts at: 3032 497
Size: 1454
Fragments arranged by size:
1454 1013 360 314 245 222 176 169
36
NaeI GCC'GGC
Cuts at: 565 2170 565
Size: 1605 2384
NarI GG'CG_CC
Cuts at: 1420 1668 1420
Size: 248 3741
NgoMIV G'CCGG_C
Cuts at: 563 2168 563
Size: 1605 2384
NspI r_CATG'y
Cuts at: 636 1042 1542 2449 636
Size: 406 500 907 2176
Fragments arranged by size:
2176 907 500 406
PasI CC'CwG_GG
Cuts at: 855 855
Size: 3989
PciI A'CATG_T
Cuts at: 2445 2445
Size: 3989
PflMI CCAn_nnn'nTGG
Cuts at: 360 360
Size: 3989
PmeI GTTT'AAAC
Cuts at: 988 988
Size: 3989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3957
PpuMI rG'GwC_Cy
Cuts at: 594 696 594
Size: 102 3887
PstI C_TGCA'G
Cuts at: 393 1036 1721 393
Size: 643 685 2661
Fragments arranged by size:
2661 685 643
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3989
PvuII CAG'CTG
Cuts at: 666 924 1414 1774 666
Size: 258 490 360 2881
Fragments arranged by size:
2881 490 360 258
RsrII CG'GwC_CG
Cuts at: 2184 2184
Size: 3989
SacI G_AGCT'C
Cuts at: 1003 1003
Size: 3989
SacII CC_GC'GG
Cuts at: 498 498
Size: 3989
SalI G'TCGA_C
Cuts at: 1026 1026
Size: 3989
SapI GCTCTTCn'nnn_
Cuts at: 2012 2222 2012
Size: 210 3779
SbfI CC_TGCA'GG
Cuts at: 1036 1036
Size: 3989
ScaI AGT'ACT
Cuts at: 3506 3506
Size: 3989
SfcI C'TryA_G
Cuts at: 33 389 1032 1717 2710 2901 33
Size: 356 643 685 993 191 1121
Fragments arranged by size:
1121 993 685 643 356 191
SfoI GGC'GCC
Cuts at: 1421 1669 1421
Size: 248 3741
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
SmaI CCC'GGG
Cuts at: 938 1011 938
Size: 73 3916
SmlI C'TyrA_G
Cuts at: 138 1144 2059 2551 2813 138
Size: 1006 915 492 262 1314
Fragments arranged by size:
1314 1006 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3989
SphI G_CATG'C
Cuts at: 636 1042 1542 636
Size: 406 500 3083
Fragments arranged by size:
3083 500 406
StyI C'CwwG_G
Cuts at: 547 1123 1477 547
Size: 576 354 3059
Fragments arranged by size:
3059 576 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 985 1572 1705 2164 2373 985
Size: 587 133 459 209 2601
Fragments arranged by size:
2601 587 459 209 133
TatI w'GTAC_w
Cuts at: 3504 3504
Size: 3989
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 3957
Tth111I GACn'n_nGTC
Cuts at: 592 1786 592
Size: 1194 2795
XbaI T'CTAG_A
Cuts at: 1020 1020
Size: 3989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 786 1484 786
Size: 698 3291
XmaI C'CCGG_G
Cuts at: 936 1009 936
Size: 73 3916
ZraI GAC'GTC
Cuts at: 768 768
Size: 3989
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BseRI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI