Restriction map for
pF1KB7445
(Circular) (Six-base) MAPSORT of: pF1KB7445.seq Check: 4920 from: 1 to: 4607
pF1KB7445 4607 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 626 1645 626
Size: 1019 3588
Acc65I G'GTAC_C
Cuts at: 1623 1623
Size: 4607
AclI AA'CG_TT
Cuts at: 4447 4447
Size: 4607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 844 929 2432 2864 3611 3879 150
Size: 694 85 1503 432 747 268 878
Fragments arranged by size:
1503 878 747 694 432 268 85
AflIII A'CryG_T
Cuts at: 1959 3063 4040 1959
Size: 1104 977 2526
Fragments arranged by size:
2526 1104 977
AgeI A'CCGG_T
Cuts at: 2995 2995
Size: 4607
AlwNI CAG_nnn'CTG
Cuts at: 1912 3479 1912
Size: 1567 3040
ApaI G_GGCC'C
Cuts at: 376 376
Size: 4607
ApaLI G'TGCA_C
Cuts at: 3377 3377
Size: 4607
ApoI r'AATT_y
Cuts at: 751 1611 3960 4216 751
Size: 860 2349 256 1142
Fragments arranged by size:
2349 1142 860 256
AseI AT'TA_AT
Cuts at: 20 643 20
Size: 623 3984
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
AvaI C'yCGr_G
Cuts at: 1627 1627
Size: 4607
BamHI G'GATC_C
Cuts at: 1632 1632
Size: 4607
BanI G'GyrC_C
Cuts at: 1623 2037 2285 2320 1623
Size: 414 248 35 3910
Fragments arranged by size:
3910 414 248 35
BanII G_rGCy'C
Cuts at: 376 1621 2651 376
Size: 1245 1030 2332
Fragments arranged by size:
2332 1245 1030
BbeI G_GCGC'C
Cuts at: 2041 2289 2041
Size: 248 4359
BbsI GAAGACnn'nnnn_
Cuts at: 448 844 448
Size: 396 4211
BbvCI CC'TCA_GC
Cuts at: 116 116
Size: 4607
BciVI GTATCCnnnnn_n'
Cuts at: 2505 3266 4607 2505
Size: 761 1341 2505
Fragments arranged by size:
2505 1341 761
BclI T'GATC_A
Cuts at: 2127 2127
Size: 4607
BglI GCCn_nnn'nGGC
Cuts at: 112 1050 112
Size: 938 3669
BglII A'GATC_T
Cuts at: 300 2122 4159 300
Size: 1822 2037 748
Fragments arranged by size:
2037 1822 748
BlpI GC'TnA_GC
Cuts at: 1719 1719
Size: 4607
Bme1580I G_kGCm'C
Cuts at: 376 2232 2325 3381 376
Size: 1856 93 1056 1602
Fragments arranged by size:
1856 1602 1056 93
BmgBI CAC'GTC
Cuts at: 288 288
Size: 4607
BmrI ACTGGGnnnn_n'
Cuts at: 1819 1900 2235 4379 1819
Size: 81 335 2144 2047
Fragments arranged by size:
2144 2047 335 81
BmtI G_CTAG'C
Cuts at: 1478 1478
Size: 4607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 370 428 527 2974 370
Size: 58 99 2447 2003
Fragments arranged by size:
2447 2003 99 58
Bpu10I CC'TnA_GC
Cuts at: 116 367 116
Size: 251 4356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1076 1783 2662 3154 3452 869
Size: 207 707 879 492 298 2024
Fragments arranged by size:
2024 879 707 492 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 242 4311 242
Size: 4069 538
BsaAI yAC'GTr
Cuts at: 1960 1960
Size: 4607
BsaBI GATnn'nnATC
Cuts at: 6 581 2121 4158 6
Size: 575 1540 2037 455
Fragments arranged by size:
2037 1540 575 455
BsaHI Gr'CG_yC
Cuts at: 2038 2286 2988 4484 2038
Size: 248 702 1496 2161
Fragments arranged by size:
2161 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1796 2317 2995 3269 3416 3827 1796
Size: 521 678 274 147 411 2576
Fragments arranged by size:
2576 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 265 295 265
Size: 30 4577
BseYI C'CCAG_C
Cuts at: 2032 2790 3367 3863 2032
Size: 758 577 496 2776
Fragments arranged by size:
2776 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2149 2195 3403 104
Size: 2045 46 1208 1308
Fragments arranged by size:
2045 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1621 2399 2589 3381 1621
Size: 778 190 792 2847
Fragments arranged by size:
2847 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 376 1621 2232 2325 2399 2589 2651 3381 376
Size: 1245 611 93 74 190 62 730 1602
Fragments arranged by size:
1602 1245 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3827 3827
Size: 4607
BspHI T'CATG_A
Cuts at: 3783 3783
Size: 4607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1657 1871 2173 2554 1657
Size: 214 302 381 3710
Fragments arranged by size:
3710 381 302 214
BsrBI CCG'CTC
Cuts at: 50 349 2899 2953 4437 4602 50
Size: 299 2550 54 1484 165 55
Fragments arranged by size:
2550 1484 299 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1212 1432 2519 1212
Size: 220 1087 3300
Fragments arranged by size:
3300 1087 220
BsrFI r'CCGG_y
Cuts at: 2605 2786 2995 2605
Size: 181 209 4217
Fragments arranged by size:
4217 209 181
BsrGI T'GTAC_A
Cuts at: 723 723
Size: 4607
BssSI C'ACGA_G
Cuts at: 2878 3236 2878
Size: 358 4249
BstAPI GCAn_nnn'nTGC
Cuts at: 188 1984 188
Size: 1796 2811
BstBI TT'CG_AA
Cuts at: 2968 2968
Size: 4607
BstYI r'GATC_y
Cuts at: 1 300 351 1632 2099 2122 2457 2703 3715
Size: 299 51 1281 467 23 335 246 1012
Cuts at: 3715 3801 3813 4159 1
Size: 86 12 346 449
Fragments arranged by size:
1281 1012 467 449 346 335 299 246
86 51 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4607
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2557 2738 2901 2557
Size: 181 163 4263
Fragments arranged by size:
4263 181 163
BtsI GCAGTG_nn'
Cuts at: 655 1850 655
Size: 1195 3412
DraI TTT'AAA
Cuts at: 779 1606 779
Size: 827 3780
DrdI GACnn_nn'nnGTC
Cuts at: 2313 3171 2313
Size: 858 3749
EaeI y'GGCC_r
Cuts at: 647 1372 2192 2366 2757 2784 4129 647
Size: 725 820 174 391 27 1345 1125
Fragments arranged by size:
1345 1125 820 725 391 174 27
EagI C'GGCC_G
Cuts at: 2192 2192
Size: 4607
EarI CTCTTCn'nnn_
Cuts at: 397 996 1034 1277 2630 2840 397
Size: 599 38 243 1353 210 2164
Fragments arranged by size:
2164 1353 599 243 210 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2815 3125 3271 4417 2815
Size: 310 146 1146 3005
Fragments arranged by size:
3005 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1619 1619
Size: 4607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 844 929 2432 2864 3611 3879 150
Size: 694 85 1503 432 747 268 878
Fragments arranged by size:
1503 878 747 694 432 268 85
EcoNI CCTnn'n_nnAGG
Cuts at: 210 210
Size: 4607
EcoO109I rG'GnC_Cy
Cuts at: 372 373 1746 372
Size: 1 1373 3233
Fragments arranged by size:
3233 1373 1
EcoRI G'AATT_C
Cuts at: 1611 1611
Size: 4607
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 174 2626 174
Size: 2452 2155
FspI TGC'GCA
Cuts at: 2388 2388
Size: 4607
HaeII r_GCGC'y
Cuts at: 266 2041 2289 3311 4290 266
Size: 1775 248 1022 979 583
Fragments arranged by size:
1775 1022 979 583 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 667 699 2449 2479 2481 2511 667
Size: 32 1750 30 2 30 2763
Fragments arranged by size:
2763 1750 32 30 30 2
HincII GTy'rAC
Cuts at: 478 1646 478
Size: 1168 3439
KasI G'GCGC_C
Cuts at: 2037 2285 2037
Size: 248 4359
KpnI G_GTAC'C
Cuts at: 1627 1627
Size: 4607
MluI A'CGCG_T
Cuts at: 4040 4040
Size: 4607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3278 3462 3278
Size: 184 4423
MscI TGG'CCA
Cuts at: 649 1374 2368 649
Size: 725 994 2888
Fragments arranged by size:
2888 994 725
MslI CAynn'nnrTG
Cuts at: 361 2596 2723 361
Size: 2235 127 2245
Fragments arranged by size:
2245 2235 127
MspA1I CmG'CkG
Cuts at: 1718 2032 2392 3405 3650 1718
Size: 314 360 1013 245 2675
Fragments arranged by size:
2675 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2788 2788
Size: 4607
NarI GG'CG_CC
Cuts at: 2038 2286 2038
Size: 248 4359
NcoI C'CATG_G
Cuts at: 107 107
Size: 4607
NgoMIV G'CCGG_C
Cuts at: 2786 2786
Size: 4607
NheI G'CTAG_C
Cuts at: 1474 1474
Size: 4607
NspI r_CATG'y
Cuts at: 1660 2160 3067 1660
Size: 500 907 3200
Fragments arranged by size:
3200 907 500
PciI A'CATG_T
Cuts at: 3063 3063
Size: 4607
PfoI T'CCnGG_A
Cuts at: 1490 1490
Size: 4607
PmeI GTTT'AAAC
Cuts at: 1606 1606
Size: 4607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4575
PspOMI G'GGCC_C
Cuts at: 372 372
Size: 4607
PstI C_TGCA'G
Cuts at: 214 1654 2339 214
Size: 1440 685 2482
Fragments arranged by size:
2482 1440 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4607
PvuII CAG'CTG
Cuts at: 2032 2392 2032
Size: 360 4247
RsrII CG'GwC_CG
Cuts at: 2802 2802
Size: 4607
SacI G_AGCT'C
Cuts at: 1621 1621
Size: 4607
SalI G'TCGA_C
Cuts at: 1644 1644
Size: 4607
SapI GCTCTTCn'nnn_
Cuts at: 1034 2630 2840 1034
Size: 1596 210 2801
Fragments arranged by size:
2801 1596 210
SbfI CC_TGCA'GG
Cuts at: 1654 1654
Size: 4607
ScaI AGT'ACT
Cuts at: 4124 4124
Size: 4607
SfcI C'TryA_G
Cuts at: 33 210 1650 2335 3328 3519 33
Size: 177 1440 685 993 191 1121
Fragments arranged by size:
1440 1121 993 685 191 177
SfoI GGC'GCC
Cuts at: 2039 2287 2039
Size: 248 4359
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
SmaI CCC'GGG
Cuts at: 1629 1629
Size: 4607
SmlI C'TyrA_G
Cuts at: 884 1091 1762 2677 3169 3431 884
Size: 207 671 915 492 262 2060
Fragments arranged by size:
2060 915 671 492 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4607
SphI G_CATG'C
Cuts at: 1660 2160 1660
Size: 500 4107
SspI AAT'ATT
Cuts at: 870 870
Size: 4607
StuI AGG'CCT
Cuts at: 1296 1296
Size: 4607
StyI C'CwwG_G
Cuts at: 107 277 1741 2095 107
Size: 170 1464 354 2619
Fragments arranged by size:
2619 1464 354 170
SwaI ATTT'AAAT
Cuts at: 779 779
Size: 4607
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 226 2190 2323 2782 2991 226
Size: 1964 133 459 209 1842
Fragments arranged by size:
1964 1842 459 209 133
TatI w'GTAC_w
Cuts at: 723 1590 4122 723
Size: 867 2532 1208
Fragments arranged by size:
2532 1208 867
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4124 4156 4124
Size: 32 4575
Tth111I GACn'n_nGTC
Cuts at: 473 2404 473
Size: 1931 2676
XbaI T'CTAG_A
Cuts at: 1638 1638
Size: 4607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2102 2102
Size: 4607
XmaI C'CCGG_G
Cuts at: 1627 1627
Size: 4607
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BpmI BseRI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI XhoI XmnI ZraI