Restriction map for
pF1KB7447
(Circular) (Six-base) MAPSORT of: pF1KB7447.seq Check: 9868 from: 1 to: 3929
pF1KB7447 3929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 967 967
Size: 3929
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 3769 3769
Size: 3929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 929 1754 2186 2933 3201 763
Size: 166 825 432 747 268 1491
Fragments arranged by size:
1491 825 747 432 268 166
AflIII A'CryG_T
Cuts at: 1281 2385 3362 1281
Size: 1104 977 1848
Fragments arranged by size:
1848 1104 977
AgeI A'CCGG_T
Cuts at: 2317 2317
Size: 3929
AleI CACnn'nnGTG
Cuts at: 559 559
Size: 3929
AlwNI CAG_nnn'CTG
Cuts at: 302 787 1234 2801 302
Size: 485 447 1567 1430
Fragments arranged by size:
1567 1430 485 447
ApaLI G'TGCA_C
Cuts at: 2699 2699
Size: 3929
ApoI r'AATT_y
Cuts at: 876 933 3282 3538 876
Size: 57 2349 256 1267
Fragments arranged by size:
2349 1267 256 57
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 949 949
Size: 3929
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 945 1359 1607 1642 945
Size: 414 248 35 3232
Fragments arranged by size:
3232 414 248 35
BanII G_rGCy'C
Cuts at: 331 409 431 943 1973 331
Size: 78 22 512 1030 2287
Fragments arranged by size:
2287 1030 512 78 22
BbeI G_GCGC'C
Cuts at: 1363 1611 1363
Size: 248 3681
BbsI GAAGACnn'nnnn_
Cuts at: 687 687
Size: 3929
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2588 3929 1827
Size: 761 1341 1827
Fragments arranged by size:
1827 1341 761
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BglII A'GATC_T
Cuts at: 1444 3481 1444
Size: 2037 1892
BlpI GC'TnA_GC
Cuts at: 1041 1041
Size: 3929
Bme1580I G_kGCm'C
Cuts at: 1554 1647 2703 1554
Size: 93 1056 2780
Fragments arranged by size:
2780 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 365 1141 1222 1557 3701 365
Size: 776 81 335 2144 593
Fragments arranged by size:
2144 776 593 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 3929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 820 199
Size: 621 3308
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1984 2476 2774 1105
Size: 879 492 298 2260
Fragments arranged by size:
2260 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 448 3633 448
Size: 3185 744
BsaAI yAC'GTr
Cuts at: 543 705 1282 543
Size: 162 577 3190
Fragments arranged by size:
3190 577 162
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 1360 1608 2310 3806 1360
Size: 248 702 1496 1483
Fragments arranged by size:
1496 1483 702 248
BsaWI w'CCGG_w
Cuts at: 473 590 1118 1639 2317 2591 2738 3149 473
Size: 117 528 521 678 274 147 411 1253
Fragments arranged by size:
1253 678 528 521 411 274 147 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 677 707 677
Size: 30 3899
BseYI C'CCAG_C
Cuts at: 1354 2112 2689 3185 1354
Size: 758 577 496 2098
Fragments arranged by size:
2098 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 336 336
Size: 3929
BsiEI CG_ry'CG
Cuts at: 104 1471 1517 2725 104
Size: 1367 46 1208 1308
Fragments arranged by size:
1367 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 409 431 943 1721 1911 2703 409
Size: 22 512 778 190 792 1635
Fragments arranged by size:
1635 792 778 512 190 22
Bsp1286I G_dGCh'C
Cuts at: 331 409 431 943 1554 1647 1721 1911 1973
Size: 78 22 512 611 93 74 190 62
Cuts at: 1973 2703 331
Size: 730 1557
Fragments arranged by size:
1557 730 611 512 190 93 78 74
62 22
BspEI T'CCGG_A
Cuts at: 473 3149 473
Size: 2676 1253
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 979 1193 1495 1876 979
Size: 214 302 381 3032
Fragments arranged by size:
3032 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2221 2275 3759 3924 50
Size: 2171 54 1484 165 55
Fragments arranged by size:
2171 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 624 1841 624
Size: 1217 2712
BsrFI r'CCGG_y
Cuts at: 1927 2108 2317 1927
Size: 181 209 3539
Fragments arranged by size:
3539 209 181
BsrGI T'GTAC_A
Cuts at: 768 768
Size: 3929
BssSI C'ACGA_G
Cuts at: 2200 2558 2200
Size: 358 3571
BstAPI GCAn_nnn'nTGC
Cuts at: 1306 1306
Size: 3929
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstXI CCAn_nnnn'nTGG
Cuts at: 798 798
Size: 3929
BstYI r'GATC_y
Cuts at: 1 954 1421 1444 1779 2025 3037 3123 3135
Size: 953 467 23 335 246 1012 86 12
Cuts at: 3135 3481 1
Size: 346 449
Fragments arranged by size:
1012 953 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 306 361 785 880 1172 306
Size: 55 424 95 292 3063
Fragments arranged by size:
3063 424 292 95 55
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 109 144 109
Size: 35 3894
DraI TTT'AAA
Cuts at: 140 928 140
Size: 788 3141
DrdI GACnn_nn'nnGTC
Cuts at: 628 1635 2493 628
Size: 1007 858 2064
Fragments arranged by size:
2064 1007 858
EaeI y'GGCC_r
Cuts at: 640 1514 1688 2079 2106 3451 640
Size: 874 174 391 27 1345 1118
Fragments arranged by size:
1345 1118 874 391 174 27
EagI C'GGCC_G
Cuts at: 1514 1514
Size: 3929
EarI CTCTTCn'nnn_
Cuts at: 317 1952 2162 317
Size: 1635 210 2084
Fragments arranged by size:
2084 1635 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2137 2447 2593 3739 2137
Size: 310 146 1146 2327
Fragments arranged by size:
2327 1146 310 146
EcoICRI GAG'CTC
Cuts at: 407 429 941 407
Size: 22 512 3395
Fragments arranged by size:
3395 512 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 763 820 929 1754 2186 2933 3201 199
Size: 564 57 109 825 432 747 268 927
Fragments arranged by size:
927 825 747 564 432 268 109 57
EcoO109I rG'GnC_Cy
Cuts at: 1068 1068
Size: 3929
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
EcoRV GAT'ATC
Cuts at: 164 164
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 459 1948 459
Size: 1489 2440
FspI TGC'GCA
Cuts at: 1710 1710
Size: 3929
HaeII r_GCGC'y
Cuts at: 1363 1611 2633 3612 1363
Size: 248 1022 979 1680
Fragments arranged by size:
1680 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 677 709 1771 1801 1803 1833 677
Size: 32 1062 30 2 30 2773
Fragments arranged by size:
2773 1062 32 30 30 2
HincII GTy'rAC
Cuts at: 968 968
Size: 3929
KasI G'GCGC_C
Cuts at: 1359 1607 1359
Size: 248 3681
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MluI A'CGCG_T
Cuts at: 3362 3362
Size: 3929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2600 2784 2600
Size: 184 3745
MscI TGG'CCA
Cuts at: 642 1690 642
Size: 1048 2881
MslI CAynn'nnrTG
Cuts at: 559 1918 2045 559
Size: 1359 127 2443
Fragments arranged by size:
2443 1359 127
MspA1I CmG'CkG
Cuts at: 598 1040 1354 1714 2727 2972 598
Size: 442 314 360 1013 245 1555
Fragments arranged by size:
1555 1013 442 360 314 245
NaeI GCC'GGC
Cuts at: 2110 2110
Size: 3929
NarI GG'CG_CC
Cuts at: 1360 1608 1360
Size: 248 3681
NgoMIV G'CCGG_C
Cuts at: 2108 2108
Size: 3929
NruI TCG'CGA
Cuts at: 550 550
Size: 3929
NspI r_CATG'y
Cuts at: 982 1482 2389 982
Size: 500 907 2522
Fragments arranged by size:
2522 907 500
PciI A'CATG_T
Cuts at: 2385 2385
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3897
PstI C_TGCA'G
Cuts at: 298 894 976 1661 298
Size: 596 82 685 2566
Fragments arranged by size:
2566 685 596 82
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 1354 1714 1354
Size: 360 3569
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 409 431 943 409
Size: 22 512 3395
Fragments arranged by size:
3395 512 22
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SfcI C'TryA_G
Cuts at: 33 294 490 890 972 1657 2650 2841 33
Size: 261 196 400 82 685 993 191 1121
Fragments arranged by size:
1121 993 685 400 261 196 191 82
SfoI GGC'GCC
Cuts at: 1361 1609 1361
Size: 248 3681
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 951 951
Size: 3929
SmlI C'TyrA_G
Cuts at: 1084 1999 2491 2753 1084
Size: 915 492 262 2260
Fragments arranged by size:
2260 915 492 262
SnaBI TAC'GTA
Cuts at: 705 705
Size: 3929
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 982 1482 982
Size: 500 3429
SspI AAT'ATT
Cuts at: 521 521
Size: 3929
StuI AGG'CCT
Cuts at: 748 748
Size: 3929
StyI C'CwwG_G
Cuts at: 1063 1417 1063
Size: 354 3575
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1512 1645 2104 2313 1512
Size: 133 459 209 3128
Fragments arranged by size:
3128 459 209 133
TatI w'GTAC_w
Cuts at: 768 3444 768
Size: 2676 1253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 3897
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1424 1424
Size: 3929
XmaI C'CCGG_G
Cuts at: 949 949
Size: 3929
XmnI GAAnn'nnTTC
Cuts at: 520 808 520
Size: 288 3641
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SwaI XhoI ZraI