Restriction map for
pF1KB7448
(Circular) (Six-base) MAPSORT of: pF1KB7448.seq Check: 2698 from: 1 to: 4085
pF1KB7448 4085 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1123 1123
Size: 4085
Acc65I G'GTAC_C
Cuts at: 351 1101 351
Size: 750 3335
AclI AA'CG_TT
Cuts at: 3925 3925
Size: 4085
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1910 2342 3089 3357 1910
Size: 432 747 268 2638
Fragments arranged by size:
2638 747 432 268
AflIII A'CryG_T
Cuts at: 838 1437 2541 3518 838
Size: 599 1104 977 1405
Fragments arranged by size:
1405 1104 977 599
AgeI A'CCGG_T
Cuts at: 2473 2473
Size: 4085
AleI CACnn'nnGTG
Cuts at: 445 940 445
Size: 495 3590
AlwNI CAG_nnn'CTG
Cuts at: 1390 2957 1390
Size: 1567 2518
ApaLI G'TGCA_C
Cuts at: 2855 2855
Size: 4085
ApoI r'AATT_y
Cuts at: 521 1089 3438 3694 521
Size: 568 2349 256 912
Fragments arranged by size:
2349 912 568 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4085
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
AvaI C'yCGr_G
Cuts at: 378 1105 378
Size: 727 3358
BamHI G'GATC_C
Cuts at: 1110 1110
Size: 4085
BanI G'GyrC_C
Cuts at: 351 1101 1515 1763 1798 351
Size: 750 414 248 35 2638
Fragments arranged by size:
2638 750 414 248 35
BanII G_rGCy'C
Cuts at: 293 745 1099 2129 293
Size: 452 354 1030 2249
Fragments arranged by size:
2249 1030 452 354
BbeI G_GCGC'C
Cuts at: 1519 1767 1519
Size: 248 3837
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4051
BciVI GTATCCnnnnn_n'
Cuts at: 178 1983 2744 4085 178
Size: 1805 761 1341 178
Fragments arranged by size:
1805 1341 761 178
BclI T'GATC_A
Cuts at: 965 1605 965
Size: 640 3445
BglII A'GATC_T
Cuts at: 1600 3637 1600
Size: 2037 2048
BlpI GC'TnA_GC
Cuts at: 1197 1197
Size: 4085
Bme1580I G_kGCm'C
Cuts at: 701 929 1710 1803 2859 701
Size: 228 781 93 1056 1927
Fragments arranged by size:
1927 1056 781 228 93
BmrI ACTGGGnnnn_n'
Cuts at: 1297 1378 1713 3857 1297
Size: 81 335 2144 1525
Fragments arranged by size:
2144 1525 335 81
BmtI G_CTAG'C
Cuts at: 952 952
Size: 4085
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1042
Size: 4085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 1261 2140 2632 2930 518
Size: 743 879 492 298 1673
Fragments arranged by size:
1673 879 743 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3789 3789
Size: 4085
BsaAI yAC'GTr
Cuts at: 963 1438 963
Size: 475 3610
BsaBI GATnn'nnATC
Cuts at: 6 1599 3636 6
Size: 1593 2037 455
Fragments arranged by size:
2037 1593 455
BsaHI Gr'CG_yC
Cuts at: 1516 1764 2466 3962 1516
Size: 248 702 1496 1639
Fragments arranged by size:
1639 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1274 1795 2473 2747 2894 3305 1274
Size: 521 678 274 147 411 2054
Fragments arranged by size:
2054 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 333 795 333
Size: 462 3623
BseYI C'CCAG_C
Cuts at: 111 543 700 972 1510 2268 2845 3341 111
Size: 432 157 272 538 758 577 496 855
Fragments arranged by size:
855 758 577 538 496 432 272 157
BsiEI CG_ry'CG
Cuts at: 104 1627 1673 2881 104
Size: 1523 46 1208 1308
Fragments arranged by size:
1523 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1099 1877 2067 2859 1099
Size: 778 190 792 2325
Fragments arranged by size:
2325 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 445 445
Size: 4085
Bsp1286I G_dGCh'C
Cuts at: 293 701 745 929 1099 1710 1803 1877 2067
Size: 408 44 184 170 611 93 74 190
Cuts at: 2067 2129 2859 293
Size: 62 730 1519
Fragments arranged by size:
1519 730 611 408 190 184 170 93
74 62 44
BspEI T'CCGG_A
Cuts at: 3305 3305
Size: 4085
BspHI T'CATG_A
Cuts at: 584 3261 584
Size: 2677 1408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1135 1349 1651 2032 1135
Size: 214 302 381 3188
Fragments arranged by size:
3188 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2377 2431 3915 4080 50
Size: 2327 54 1484 165 55
Fragments arranged by size:
2327 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 758 1997 758
Size: 1239 2846
BsrFI r'CCGG_y
Cuts at: 2083 2264 2473 2083
Size: 181 209 3695
Fragments arranged by size:
3695 209 181
BsrGI T'GTAC_A
Cuts at: 221 221
Size: 4085
BssSI C'ACGA_G
Cuts at: 2356 2714 2356
Size: 358 3727
BstAPI GCAn_nnn'nTGC
Cuts at: 374 1462 374
Size: 1088 2997
BstBI TT'CG_AA
Cuts at: 2446 2446
Size: 4085
BstXI CCAn_nnnn'nTGG
Cuts at: 447 599 447
Size: 152 3933
BstYI r'GATC_y
Cuts at: 1 1110 1577 1600 1935 2181 3193 3279 3291
Size: 1109 467 23 335 246 1012 86 12
Cuts at: 3291 3637 1
Size: 346 449
Fragments arranged by size:
1109 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 347 470 347
Size: 123 3962
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2035 2216 2379 2035
Size: 181 163 3741
Fragments arranged by size:
3741 181 163
BtsI GCAGTG_nn'
Cuts at: 1328 1328
Size: 4085
DraI TTT'AAA
Cuts at: 983 1084 983
Size: 101 3984
DraIII CAC_nnn'GTG
Cuts at: 214 214
Size: 4085
DrdI GACnn_nn'nnGTC
Cuts at: 1791 2649 1791
Size: 858 3227
EaeI y'GGCC_r
Cuts at: 557 671 1670 1844 2235 2262 3607 557
Size: 114 999 174 391 27 1345 1035
Fragments arranged by size:
1345 1035 999 391 174 114 27
EagI C'GGCC_G
Cuts at: 1670 1670
Size: 4085
EarI CTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2293 2603 2749 3895 2293
Size: 310 146 1146 2483
Fragments arranged by size:
2483 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1097 1097
Size: 4085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1910 2342 3089 3357 1042
Size: 868 432 747 268 1770
Fragments arranged by size:
1770 868 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1224 1224
Size: 4085
EcoRI G'AATT_C
Cuts at: 521 1089 521
Size: 568 3517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 4085
FspI TGC'GCA
Cuts at: 1866 1866
Size: 4085
HaeII r_GCGC'y
Cuts at: 1519 1767 2789 3768 1519
Size: 248 1022 979 1836
Fragments arranged by size:
1836 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 594 597 626 629 790 822 1927 1957 1959
Size: 3 29 3 161 32 1105 30 2
Cuts at: 1959 1989 594
Size: 30 2690
Fragments arranged by size:
2690 1105 161 32 30 30 29 3
3 2
HincII GTy'rAC
Cuts at: 1124 1124
Size: 4085
KasI G'GCGC_C
Cuts at: 1515 1763 1515
Size: 248 3837
KpnI G_GTAC'C
Cuts at: 355 1105 355
Size: 750 3335
MfeI C'AATT_G
Cuts at: 1015 1015
Size: 4085
MluI A'CGCG_T
Cuts at: 3518 3518
Size: 4085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 580 1091 2756 2940 580
Size: 511 1665 184 1725
Fragments arranged by size:
1725 1665 511 184
MscI TGG'CCA
Cuts at: 673 1846 673
Size: 1173 2912
MslI CAynn'nnrTG
Cuts at: 334 418 445 583 940 2074 2201 334
Size: 84 27 138 357 1134 127 2218
Fragments arranged by size:
2218 1134 357 138 127 84 27
MspA1I CmG'CkG
Cuts at: 445 1196 1510 1870 2883 3128 445
Size: 751 314 360 1013 245 1402
Fragments arranged by size:
1402 1013 751 360 314 245
NaeI GCC'GGC
Cuts at: 2266 2266
Size: 4085
NarI GG'CG_CC
Cuts at: 1516 1764 1516
Size: 248 3837
NcoI C'CATG_G
Cuts at: 470 470
Size: 4085
NgoMIV G'CCGG_C
Cuts at: 2264 2264
Size: 4085
NheI G'CTAG_C
Cuts at: 948 948
Size: 4085
NspI r_CATG'y
Cuts at: 686 842 1138 1638 2545 686
Size: 156 296 500 907 2226
Fragments arranged by size:
2226 907 500 296 156
PciI A'CATG_T
Cuts at: 838 2541 838
Size: 1703 2382
PflMI CCAn_nnn'nTGG
Cuts at: 135 135
Size: 4085
PmeI GTTT'AAAC
Cuts at: 1084 1084
Size: 4085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 4053
PstI C_TGCA'G
Cuts at: 1132 1817 1132
Size: 685 3400
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4085
PvuII CAG'CTG
Cuts at: 445 1510 1870 445
Size: 1065 360 2660
Fragments arranged by size:
2660 1065 360
RsrII CG'GwC_CG
Cuts at: 2280 2280
Size: 4085
SacI G_AGCT'C
Cuts at: 1099 1099
Size: 4085
SalI G'TCGA_C
Cuts at: 1122 1122
Size: 4085
SapI GCTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
SbfI CC_TGCA'GG
Cuts at: 1132 1132
Size: 4085
ScaI AGT'ACT
Cuts at: 3602 3602
Size: 4085
SfcI C'TryA_G
Cuts at: 33 711 1128 1813 2806 2997 33
Size: 678 417 685 993 191 1121
Fragments arranged by size:
1121 993 685 678 417 191
SfoI GGC'GCC
Cuts at: 1517 1765 1517
Size: 248 3837
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
SmaI CCC'GGG
Cuts at: 1107 1107
Size: 4085
SmlI C'TyrA_G
Cuts at: 533 1240 2155 2647 2909 533
Size: 707 915 492 262 1709
Fragments arranged by size:
1709 915 707 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4085
SphI G_CATG'C
Cuts at: 1138 1638 1138
Size: 500 3585
StyI C'CwwG_G
Cuts at: 470 1219 1573 470
Size: 749 354 2982
Fragments arranged by size:
2982 749 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 952 1668 1801 2260 2469 952
Size: 716 133 459 209 2568
Fragments arranged by size:
2568 716 459 209 133
TatI w'GTAC_w
Cuts at: 221 869 3600 221
Size: 648 2731 706
Fragments arranged by size:
2731 706 648
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 969 1001 3602 3634 969
Size: 32 2601 32 1420
Fragments arranged by size:
2601 1420 32 32
Tth111I GACn'n_nGTC
Cuts at: 1882 1882
Size: 4085
XbaI T'CTAG_A
Cuts at: 1116 1116
Size: 4085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1017 1580 1017
Size: 563 3522
XmaI C'CCGG_G
Cuts at: 1105 1105
Size: 4085
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
Bpu10I BsaXI BsgI BsiWI BsmI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI