(Circular) (Six-base) MAPSORT of: pF1KB7449.seq  Check: 4902  from: 1  to: 3677



pF1KB7449  3677 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AccI GT'mk_AC

Cuts at:    715     715 
   Size:       3677
 
Acc65I G'GTAC_C

Cuts at:    693     693 
   Size:       3677
 
AclI AA'CG_TT

Cuts at:   3517    3517 
   Size:       3677
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1502    1934    2681    2949    1502 
   Size:        432     747     268    2230

  Fragments arranged by size: 

                2230     747     432     268
 
AflIII A'CryG_T

Cuts at:   1029    2133    3110    1029 
   Size:       1104     977    1596

  Fragments arranged by size: 

                1596    1104     977
 
AgeI A'CCGG_T

Cuts at:   2065    2065 
   Size:       3677
 
AlwNI CAG_nnn'CTG

Cuts at:    982    2549     982 
   Size:       1567    2110
 
ApaLI G'TGCA_C

Cuts at:   2447    2447 
   Size:       3677
 
ApoI r'AATT_y

Cuts at:    229     681    3030    3286     229 
   Size:        452    2349     256     620

  Fragments arranged by size: 

                2349     620     452     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3677
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3677
 
AvaI C'yCGr_G

Cuts at:    445     697     445 
   Size:        252    3425
 
BamHI G'GATC_C

Cuts at:    702     702 
   Size:       3677
 
BanI G'GyrC_C

Cuts at:    693    1107    1355    1390     693 
   Size:        414     248      35    2980

  Fragments arranged by size: 

                2980     414     248      35
 
BanII G_rGCy'C

Cuts at:    691    1721     691 
   Size:       1030    2647
 
BbeI G_GCGC'C

Cuts at:   1111    1359    1111 
   Size:        248    3429
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    109     143     162     172     196     206     423     457     465 
   Size:         34      19      10      24      10     217      34       8

Cuts at:    465     499     109 
   Size:         34    3287

  Fragments arranged by size: 

                3287     217      34      34      34      24      19      10
                  10       8
 
BciVI GTATCCnnnnn_n'

Cuts at:   1575    2336    3677    1575 
   Size:        761    1341    1575

  Fragments arranged by size: 

                1575    1341     761
 
BclI T'GATC_A

Cuts at:   1197    1197 
   Size:       3677
 
BglII A'GATC_T

Cuts at:    568    1192    3229     568 
   Size:        624    2037    1016

  Fragments arranged by size: 

                2037    1016     624
 
BlpI GC'TnA_GC

Cuts at:    789     789 
   Size:       3677
 
Bme1580I G_kGCm'C

Cuts at:    529    1302    1395    2451     529 
   Size:        773      93    1056    1755

  Fragments arranged by size: 

                1755    1056     773      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    889     970    1305    3449     889 
   Size:         81     335    2144    1117

  Fragments arranged by size: 

                2144    1117     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2044    2044 
   Size:       3677
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    403     403 
   Size:       3677
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    853    1732    2224    2522     853 
   Size:        879     492     298    2008

  Fragments arranged by size: 

                2008     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    129    3381     129 
   Size:       3252     425
 
BsaAI yAC'GTr

Cuts at:   1030    1030 
   Size:       3677
 
BsaBI GATnn'nnATC

Cuts at:      6    1191    3228       6 
   Size:       1185    2037     455

  Fragments arranged by size: 

                2037    1185     455
 
BsaHI Gr'CG_yC

Cuts at:    122    1108    1356    2058    3554     122 
   Size:        986     248     702    1496     245

  Fragments arranged by size: 

                1496     986     702     248     245
 
BsaWI w'CCGG_w

Cuts at:    866    1387    2065    2339    2486    2897     866 
   Size:        521     678     274     147     411    1646

  Fragments arranged by size: 

                1646     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1102    1860    2437    2933    1102 
   Size:        758     577     496    1846

  Fragments arranged by size: 

                1846     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1219    1265    2473     104 
   Size:       1115      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1115      46
 
BsiHKAI G_wGCw'C

Cuts at:    691    1469    1659    2451     691 
   Size:        778     190     792    1917

  Fragments arranged by size: 

                1917     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    110     110 
   Size:       3677
 
Bsp1286I G_dGCh'C

Cuts at:    529     691    1302    1395    1469    1659    1721    2451     529 
   Size:        162     611      93      74     190      62     730    1755

  Fragments arranged by size: 

                1755     730     611     190     162      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2897    2897 
   Size:       3677
 
BspHI T'CATG_A

Cuts at:   2853    2853 
   Size:       3677
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    727     941    1243    1624     727 
   Size:        214     302     381    2780

  Fragments arranged by size: 

                2780     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1969    2023    3507    3672      50 
   Size:       1919      54    1484     165      55

  Fragments arranged by size: 

                1919    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1589    1589 
   Size:       3677
 
BsrFI r'CCGG_y

Cuts at:   1675    1856    2065    1675 
   Size:        181     209    3287

  Fragments arranged by size: 

                3287     209     181
 
BssSI C'ACGA_G

Cuts at:    562    1948    2306     562 
   Size:       1386     358    1933

  Fragments arranged by size: 

                1933    1386     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1054    1054 
   Size:       3677
 
BstBI TT'CG_AA

Cuts at:    475    2038     475 
   Size:       1563    2114
 
BstYI r'GATC_y

Cuts at:      1     568     702    1169    1192    1527    1773    2785    2871 
   Size:        567     134     467      23     335     246    1012      86

Cuts at:   2871    2883    3229       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     567     467     449     346     335     246     134
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    656     656 
   Size:       3677
 
BtgI C'CryG_G

Cuts at:    375     375 
   Size:       3677
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1627    1808    1971    1627 
   Size:        181     163    3333

  Fragments arranged by size: 

                3333     181     163
 
BtsI GCAGTG_nn'

Cuts at:    920     920 
   Size:       3677
 
DraI TTT'AAA

Cuts at:    676     676 
   Size:       3677
 
DrdI GACnn_nn'nnGTC

Cuts at:   1383    2241    1383 
   Size:        858    2819
 
EaeI y'GGCC_r

Cuts at:   1262    1436    1827    1854    3199    1262 
   Size:        174     391      27    1345    1740

  Fragments arranged by size: 

                1740    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1262    1262 
   Size:       3677
 
EarI CTCTTCn'nnn_

Cuts at:    160     206     256     635    1700    1910     160 
   Size:         46      50     379    1065     210    1927

  Fragments arranged by size: 

                1927    1065     379     210      50      46
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1885    2195    2341    3487    1885 
   Size:        310     146    1146    2075

  Fragments arranged by size: 

                2075    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    689     689 
   Size:       3677
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    403    1502    1934    2681    2949     403 
   Size:       1099     432     747     268    1131

  Fragments arranged by size: 

                1131    1099     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    816     816 
   Size:       3677
 
EcoRI G'AATT_C

Cuts at:    681     681 
   Size:       3677
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1696    1696 
   Size:       3677
 
FspI TGC'GCA

Cuts at:   1458    1458 
   Size:       3677
 
HaeII r_GCGC'y

Cuts at:   1111    1359    2381    3360    1111 
   Size:        248    1022     979    1428

  Fragments arranged by size: 

                1428    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1519    1549    1551    1581    1519 
   Size:         30       2      30    3615

  Fragments arranged by size: 

                3615      30      30       2
 
HincII GTy'rAC

Cuts at:    716     716 
   Size:       3677
 
KasI G'GCGC_C

Cuts at:   1107    1355    1107 
   Size:        248    3429
 
KpnI G_GTAC'C

Cuts at:    697     697 
   Size:       3677
 
MluI A'CGCG_T

Cuts at:   3110    3110 
   Size:       3677
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2348    2532    2348 
   Size:        184    3493
 
MscI TGG'CCA

Cuts at:   1438    1438 
   Size:       3677
 
MslI CAynn'nnrTG

Cuts at:   1666    1793    1666 
   Size:        127    3550
 
MspA1I CmG'CkG

Cuts at:    248     627     788    1102    1462    2475    2720     248 
   Size:        379     161     314     360    1013     245    1205

  Fragments arranged by size: 

                1205    1013     379     360     314     245     161
 
NaeI GCC'GGC

Cuts at:   1858    1858 
   Size:       3677
 
NarI GG'CG_CC

Cuts at:   1108    1356    1108 
   Size:        248    3429
 
NdeI CA'TA_TG

Cuts at:    257     257 
   Size:       3677
 
NgoMIV G'CCGG_C

Cuts at:   1856    1856 
   Size:       3677
 
NspI r_CATG'y

Cuts at:    595     730    1230    2137     595 
   Size:        135     500     907    2135

  Fragments arranged by size: 

                2135     907     500     135
 
PasI CC'CwG_GG

Cuts at:    140     140 
   Size:       3677
 
PciI A'CATG_T

Cuts at:   2133    2133 
   Size:       3677
 
PmeI GTTT'AAAC

Cuts at:    676     676 
   Size:       3677
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2816    2848    2816 
   Size:         32    3645
 
PstI C_TGCA'G

Cuts at:    724    1409     724 
   Size:        685    2992
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3677
 
PvuII CAG'CTG

Cuts at:    248     627    1102    1462     248 
   Size:        379     475     360    2463

  Fragments arranged by size: 

                2463     475     379     360
 
RsrII CG'GwC_CG

Cuts at:   1872    1872 
   Size:       3677
 
SacI G_AGCT'C

Cuts at:    691     691 
   Size:       3677
 
SalI G'TCGA_C

Cuts at:    714     714 
   Size:       3677
 
SapI GCTCTTCn'nnn_

Cuts at:   1700    1910    1700 
   Size:        210    3467
 
SbfI CC_TGCA'GG

Cuts at:    724     724 
   Size:       3677
 
ScaI AGT'ACT

Cuts at:   3194    3194 
   Size:       3677
 
SfcI C'TryA_G

Cuts at:     33     720    1405    2398    2589      33 
   Size:        687     685     993     191    1121

  Fragments arranged by size: 

                1121     993     687     685     191
 
SfoI GGC'GCC

Cuts at:   1109    1357    1109 
   Size:        248    3429
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3677
 
SmaI CCC'GGG

Cuts at:    699     699 
   Size:       3677
 
SmlI C'TyrA_G

Cuts at:    832    1747    2239    2501     832 
   Size:        915     492     262    2008

  Fragments arranged by size: 

                2008     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3677
 
SphI G_CATG'C

Cuts at:    730    1230     730 
   Size:        500    3177
 
StuI AGG'CCT

Cuts at:    178     178 
   Size:       3677
 
StyI C'CwwG_G

Cuts at:    811    1165     811 
   Size:        354    3323
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1260    1393    1852    2061    1260 
   Size:        133     459     209    2876

  Fragments arranged by size: 

                2876     459     209     133
 
TatI w'GTAC_w

Cuts at:   3192    3192 
   Size:       3677
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3194    3226    3194 
   Size:         32    3645
 
Tth111I GACn'n_nGTC

Cuts at:   1474    1474 
   Size:       3677
 
XbaI T'CTAG_A

Cuts at:    708     708 
   Size:       3677
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1172    1172 
   Size:       3677
 
XmaI C'CCGG_G

Cuts at:    697     697 
   Size:       3677
 
XmnI GAAnn'nnTTC

Cuts at:    471     471 
   Size:       3677
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NspI     PasI     PciI     PmeI
     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI
    BmgBI     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI
     ZraI