Restriction map for
pF1KB7451
(Circular) (Six-base) MAPSORT of: pF1KB7451.seq Check: 1071 from: 1 to: 4361
pF1KB7451 4361 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 560 1052 1399 560
Size: 492 347 3522
Fragments arranged by size:
3522 492 347
Acc65I G'GTAC_C
Cuts at: 1377 1377
Size: 4361
AclI AA'CG_TT
Cuts at: 4201 4201
Size: 4361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2186 2618 3365 3633 2186
Size: 432 747 268 2914
Fragments arranged by size:
2914 747 432 268
AflII C'TTAA_G
Cuts at: 1156 1156
Size: 4361
AflIII A'CryG_T
Cuts at: 1713 2817 3794 1713
Size: 1104 977 2280
Fragments arranged by size:
2280 1104 977
AgeI A'CCGG_T
Cuts at: 2749 2749
Size: 4361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 943 943
Size: 4361
AlwNI CAG_nnn'CTG
Cuts at: 1666 3233 1666
Size: 1567 2794
ApaLI G'TGCA_C
Cuts at: 3131 3131
Size: 4361
ApoI r'AATT_y
Cuts at: 1086 1365 3714 3970 1086
Size: 279 2349 256 1477
Fragments arranged by size:
2349 1477 279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
AvaI C'yCGr_G
Cuts at: 648 1381 648
Size: 733 3628
BamHI G'GATC_C
Cuts at: 711 1233 1386 711
Size: 522 153 3686
Fragments arranged by size:
3686 522 153
BanI G'GyrC_C
Cuts at: 117 313 1377 1791 2039 2074 117
Size: 196 1064 414 248 35 2404
Fragments arranged by size:
2404 1064 414 248 196 35
BanII G_rGCy'C
Cuts at: 129 353 902 1375 2405 129
Size: 224 549 473 1030 2085
Fragments arranged by size:
2085 1030 549 473 224
BbeI G_GCGC'C
Cuts at: 121 1795 2043 121
Size: 1674 248 2439
Fragments arranged by size:
2439 1674 248
BbsI GAAGACnn'nnnn_
Cuts at: 254 254
Size: 4361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 382 416 90
Size: 34 258 34 4035
Fragments arranged by size:
4035 258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3020 4361 2259
Size: 761 1341 2259
Fragments arranged by size:
2259 1341 761
BclI T'GATC_A
Cuts at: 1881 1881
Size: 4361
BglII A'GATC_T
Cuts at: 1876 3913 1876
Size: 2037 2324
BlpI GC'TnA_GC
Cuts at: 352 1473 352
Size: 1121 3240
Bme1580I G_kGCm'C
Cuts at: 932 1986 2079 3135 932
Size: 1054 93 1056 2158
Fragments arranged by size:
2158 1056 1054 93
BmgBI CAC'GTC
Cuts at: 708 708
Size: 4361
BmrI ACTGGGnnnn_n'
Cuts at: 919 1573 1654 1989 4133 919
Size: 654 81 335 2144 1147
Fragments arranged by size:
2144 1147 654 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 451 2728 451
Size: 2277 2084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 880 367
Size: 513 3848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 474 1537 2416 2908 3206 231
Size: 243 1063 879 492 298 1386
Fragments arranged by size:
1386 1063 879 492 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 4065 4065
Size: 4361
BsaAI yAC'GTr
Cuts at: 1714 1714
Size: 4361
BsaBI GATnn'nnATC
Cuts at: 6 1875 3912 6
Size: 1869 2037 455
Fragments arranged by size:
2037 1869 455
BsaHI Gr'CG_yC
Cuts at: 118 1224 1792 2040 2742 4238 118
Size: 1106 568 248 702 1496 241
Fragments arranged by size:
1496 1106 702 568 248 241
BsaWI w'CCGG_w
Cuts at: 1550 2071 2749 3023 3170 3581 1550
Size: 521 678 274 147 411 2330
Fragments arranged by size:
2330 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 243 826 829 832 835 243
Size: 583 3 3 3 3769
Fragments arranged by size:
3769 583 3 3 3
BseYI C'CCAG_C
Cuts at: 389 759 1786 2544 3121 3617 389
Size: 370 1027 758 577 496 1133
Fragments arranged by size:
1133 1027 758 577 496 370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 417 1216 168
Size: 249 799 3313
Fragments arranged by size:
3313 799 249
BsiEI CG_ry'CG
Cuts at: 104 308 1903 1949 3157 104
Size: 204 1595 46 1208 1308
Fragments arranged by size:
1595 1308 1208 204 46
BsiHKAI G_wGCw'C
Cuts at: 129 353 515 902 1375 2153 2343 3135 129
Size: 224 162 387 473 778 190 792 1355
Fragments arranged by size:
1355 792 778 473 387 224 190 162
BsmBI CGTCTCn'nnnn_
Cuts at: 1101 1101
Size: 4361
Bsp1286I G_dGCh'C
Cuts at: 129 353 515 902 932 1375 1986 2079 2153
Size: 224 162 387 30 443 611 93 74
Cuts at: 2153 2343 2405 3135 129
Size: 190 62 730 1355
Fragments arranged by size:
1355 730 611 443 387 224 190 162
93 74 62 30
BspEI T'CCGG_A
Cuts at: 3581 3581
Size: 4361
BspHI T'CATG_A
Cuts at: 3537 3537
Size: 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 650 1411 1625 1927 2308 650
Size: 761 214 302 381 2703
Fragments arranged by size:
2703 761 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1277 2653 2707 4191 4356 50
Size: 1227 1376 54 1484 165 55
Fragments arranged by size:
1484 1376 1227 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2273 2273
Size: 4361
BsrFI r'CCGG_y
Cuts at: 310 316 2359 2540 2749 310
Size: 6 2043 181 209 1922
Fragments arranged by size:
2043 1922 209 181 6
BssSI C'ACGA_G
Cuts at: 122 2632 2990 122
Size: 2510 358 1493
Fragments arranged by size:
2510 1493 358
BstAPI GCAn_nnn'nTGC
Cuts at: 579 1738 579
Size: 1159 3202
BstBI TT'CG_AA
Cuts at: 2722 2722
Size: 4361
BstYI r'GATC_y
Cuts at: 1 711 1233 1386 1853 1876 2211 2457 3469
Size: 710 522 153 467 23 335 246 1012
Cuts at: 3469 3555 3567 3913 1
Size: 86 12 346 449
Fragments arranged by size:
1012 710 522 467 449 346 335 246
153 86 23 12
BtgI C'CryG_G
Cuts at: 777 777
Size: 4361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2311 2492 2655 2311
Size: 181 163 4017
Fragments arranged by size:
4017 181 163
BtsI GCAGTG_nn'
Cuts at: 400 799 877 1604 400
Size: 399 78 727 3157
Fragments arranged by size:
3157 727 399 78
DraI TTT'AAA
Cuts at: 1360 1360
Size: 4361
DrdI GACnn_nn'nnGTC
Cuts at: 2067 2925 2067
Size: 858 3503
EaeI y'GGCC_r
Cuts at: 305 693 701 780 1946 2120 2511 2538 3883
Size: 388 8 79 1166 174 391 27 1345
Cuts at: 3883 305
Size: 783
Fragments arranged by size:
1345 1166 783 391 388 174 79 27
8
EagI C'GGCC_G
Cuts at: 305 1946 305
Size: 1641 2720
EarI CTCTTCn'nnn_
Cuts at: 803 890 2384 2594 803
Size: 87 1494 210 2570
Fragments arranged by size:
2570 1494 210 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2569 2879 3025 4171 2569
Size: 310 146 1146 2759
Fragments arranged by size:
2759 1146 310 146
EcoICRI GAG'CTC
Cuts at: 127 351 900 1373 127
Size: 224 549 473 3115
Fragments arranged by size:
3115 549 473 224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 880 2186 2618 3365 3633 367
Size: 513 1306 432 747 268 1095
Fragments arranged by size:
1306 1095 747 513 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 609 609
Size: 4361
EcoO109I rG'GnC_Cy
Cuts at: 1213 1500 1213
Size: 287 4074
EcoRI G'AATT_C
Cuts at: 1365 1365
Size: 4361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4361
FspI TGC'GCA
Cuts at: 2142 2142
Size: 4361
HaeII r_GCGC'y
Cuts at: 121 341 1795 2043 3065 4044 121
Size: 220 1454 248 1022 979 438
Fragments arranged by size:
1454 1022 979 438 248 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 703 735 1112 1144 2203 2233 2235 2265 703
Size: 32 377 32 1059 30 2 30 2799
Fragments arranged by size:
2799 1059 377 32 32 30 30 2
HincII GTy'rAC
Cuts at: 722 1400 722
Size: 678 3683
HindIII A'AGCT_T
Cuts at: 654 654
Size: 4361
KasI G'GCGC_C
Cuts at: 117 1791 2039 117
Size: 1674 248 2439
Fragments arranged by size:
2439 1674 248
KpnI G_GTAC'C
Cuts at: 1381 1381
Size: 4361
MluI A'CGCG_T
Cuts at: 3794 3794
Size: 4361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 470 685 3032 3216 470
Size: 215 2347 184 1615
Fragments arranged by size:
2347 1615 215 184
MscI TGG'CCA
Cuts at: 695 782 2122 695
Size: 87 1340 2934
Fragments arranged by size:
2934 1340 87
MslI CAynn'nnrTG
Cuts at: 568 925 2350 2477 568
Size: 357 1425 127 2452
Fragments arranged by size:
2452 1425 357 127
MspA1I CmG'CkG
Cuts at: 274 1003 1032 1472 1786 2146 3159 3404 274
Size: 729 29 440 314 360 1013 245 1231
Fragments arranged by size:
1231 1013 729 440 360 314 245 29
NaeI GCC'GGC
Cuts at: 312 2542 312
Size: 2230 2131
NarI GG'CG_CC
Cuts at: 118 1792 2040 118
Size: 1674 248 2439
Fragments arranged by size:
2439 1674 248
NcoI C'CATG_G
Cuts at: 777 777
Size: 4361
NgoMIV G'CCGG_C
Cuts at: 310 2540 310
Size: 2230 2131
NotI GC'GGCC_GC
Cuts at: 305 305
Size: 4361
NspI r_CATG'y
Cuts at: 1253 1414 1914 2821 1253
Size: 161 500 907 2793
Fragments arranged by size:
2793 907 500 161
PasI CC'CwG_GG
Cuts at: 201 201
Size: 4361
PciI A'CATG_T
Cuts at: 2817 2817
Size: 4361
PflMI CCAn_nnn'nTGG
Cuts at: 858 858
Size: 4361
PfoI T'CCnGG_A
Cuts at: 437 437
Size: 4361
PmeI GTTT'AAAC
Cuts at: 1360 1360
Size: 4361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 4329
PpuMI rG'GwC_Cy
Cuts at: 1213 1213
Size: 4361
PstI C_TGCA'G
Cuts at: 971 1408 2093 971
Size: 437 685 3239
Fragments arranged by size:
3239 685 437
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4361
PvuII CAG'CTG
Cuts at: 1003 1032 1786 2146 1003
Size: 29 754 360 3218
Fragments arranged by size:
3218 754 360 29
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 4361
SacI G_AGCT'C
Cuts at: 129 353 902 1375 129
Size: 224 549 473 3115
Fragments arranged by size:
3115 549 473 224
SalI G'TCGA_C
Cuts at: 1398 1398
Size: 4361
SapI GCTCTTCn'nnn_
Cuts at: 890 2384 2594 890
Size: 1494 210 2657
Fragments arranged by size:
2657 1494 210
SbfI CC_TGCA'GG
Cuts at: 1408 1408
Size: 4361
ScaI AGT'ACT
Cuts at: 1162 3878 1162
Size: 2716 1645
SfcI C'TryA_G
Cuts at: 33 967 1404 2089 3082 3273 33
Size: 934 437 685 993 191 1121
Fragments arranged by size:
1121 993 934 685 437 191
SfoI GGC'GCC
Cuts at: 119 1793 2041 119
Size: 1674 248 2439
Fragments arranged by size:
2439 1674 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
SgrAI Cr'CCGG_yG
Cuts at: 316 316
Size: 4361
SmaI CCC'GGG
Cuts at: 1383 1383
Size: 4361
SmlI C'TyrA_G
Cuts at: 210 489 1156 1516 2431 2923 3185 210
Size: 279 667 360 915 492 262 1386
Fragments arranged by size:
1386 915 667 492 360 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4361
SphI G_CATG'C
Cuts at: 1414 1914 1414
Size: 500 3861
StyI C'CwwG_G
Cuts at: 777 1173 1495 1849 777
Size: 396 322 354 3289
Fragments arranged by size:
3289 396 354 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 800 1944 2077 2536 2745 800
Size: 1144 133 459 209 2416
Fragments arranged by size:
2416 1144 459 209 133
TatI w'GTAC_w
Cuts at: 1160 3876 1160
Size: 2716 1645
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 4329
Tth111I GACn'n_nGTC
Cuts at: 2158 2158
Size: 4361
XbaI T'CTAG_A
Cuts at: 1392 1392
Size: 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1856 1856
Size: 4361
XmaI C'CCGG_G
Cuts at: 1381 1381
Size: 4361
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsaXI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NruI NsiI PacI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI