Restriction map for
pF1KB7501
(Circular) (Six-base) MAPSORT of: pF1KB7501.seq Check: 6892 from: 1 to: 3287
pF1KB7501 3287 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 325 325
Size: 3287
Acc65I G'GTAC_C
Cuts at: 303 303
Size: 3287
AclI AA'CG_TT
Cuts at: 3127 3127
Size: 3287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1112 1544 2291 2559 1112
Size: 432 747 268 1840
Fragments arranged by size:
1840 747 432 268
AflIII A'CryG_T
Cuts at: 639 1743 2720 639
Size: 1104 977 1206
Fragments arranged by size:
1206 1104 977
AgeI A'CCGG_T
Cuts at: 1675 1675
Size: 3287
AlwNI CAG_nnn'CTG
Cuts at: 592 2159 592
Size: 1567 1720
ApaLI G'TGCA_C
Cuts at: 2057 2057
Size: 3287
ApoI r'AATT_y
Cuts at: 291 2640 2896 291
Size: 2349 256 682
Fragments arranged by size:
2349 682 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3287
AvaI C'yCGr_G
Cuts at: 278 307 278
Size: 29 3258
BamHI G'GATC_C
Cuts at: 201 312 201
Size: 111 3176
BanI G'GyrC_C
Cuts at: 303 717 965 1000 303
Size: 414 248 35 2590
Fragments arranged by size:
2590 414 248 35
BanII G_rGCy'C
Cuts at: 301 1331 301
Size: 1030 2257
BbeI G_GCGC'C
Cuts at: 721 969 721
Size: 248 3039
BciVI GTATCCnnnnn_n'
Cuts at: 220 1185 1946 3287 220
Size: 965 761 1341 220
Fragments arranged by size:
1341 965 761 220
BclI T'GATC_A
Cuts at: 807 807
Size: 3287
BglII A'GATC_T
Cuts at: 802 2839 802
Size: 2037 1250
BlpI GC'TnA_GC
Cuts at: 399 399
Size: 3287
Bme1580I G_kGCm'C
Cuts at: 912 1005 2061 912
Size: 93 1056 2138
Fragments arranged by size:
2138 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 499 580 915 3059 499
Size: 81 335 2144 727
Fragments arranged by size:
2144 727 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1654 1654
Size: 3287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1342 1834 2132 463
Size: 879 492 298 1618
Fragments arranged by size:
1618 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2991 2991
Size: 3287
BsaAI yAC'GTr
Cuts at: 640 640
Size: 3287
BsaBI GATnn'nnATC
Cuts at: 6 157 801 2838 6
Size: 151 644 2037 455
Fragments arranged by size:
2037 644 455 151
BsaHI Gr'CG_yC
Cuts at: 718 966 1668 3164 718
Size: 248 702 1496 841
Fragments arranged by size:
1496 841 702 248
BsaWI w'CCGG_w
Cuts at: 476 997 1675 1949 2096 2507 476
Size: 521 678 274 147 411 1256
Fragments arranged by size:
1256 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 712 1470 2047 2543 712
Size: 758 577 496 1456
Fragments arranged by size:
1456 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 829 875 2083 104
Size: 725 46 1208 1308
Fragments arranged by size:
1308 1208 725 46
BsiHKAI G_wGCw'C
Cuts at: 301 1079 1269 2061 301
Size: 778 190 792 1527
Fragments arranged by size:
1527 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 301 912 1005 1079 1269 1331 2061 301
Size: 611 93 74 190 62 730 1527
Fragments arranged by size:
1527 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2507 2507
Size: 3287
BspHI T'CATG_A
Cuts at: 2463 2463
Size: 3287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 337 551 853 1234 337
Size: 214 302 381 2390
Fragments arranged by size:
2390 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1579 1633 3117 3282 50
Size: 1529 54 1484 165 55
Fragments arranged by size:
1529 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1199 1199
Size: 3287
BsrFI r'CCGG_y
Cuts at: 1285 1466 1675 1285
Size: 181 209 2897
Fragments arranged by size:
2897 209 181
BsrGI T'GTAC_A
Cuts at: 239 239
Size: 3287
BssSI C'ACGA_G
Cuts at: 1558 1916 1558
Size: 358 2929
BstAPI GCAn_nnn'nTGC
Cuts at: 664 664
Size: 3287
BstBI TT'CG_AA
Cuts at: 1648 1648
Size: 3287
BstYI r'GATC_y
Cuts at: 1 201 312 779 802 1137 1383 2395 2481
Size: 200 111 467 23 335 246 1012 86
Cuts at: 2481 2493 2839 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 200 111
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1237 1418 1581 1237
Size: 181 163 2943
Fragments arranged by size:
2943 181 163
BtsI GCAGTG_nn'
Cuts at: 530 530
Size: 3287
DraI TTT'AAA
Cuts at: 286 286
Size: 3287
DrdI GACnn_nn'nnGTC
Cuts at: 993 1851 993
Size: 858 2429
EaeI y'GGCC_r
Cuts at: 872 1046 1437 1464 2809 872
Size: 174 391 27 1345 1350
Fragments arranged by size:
1350 1345 391 174 27
EagI C'GGCC_G
Cuts at: 872 872
Size: 3287
EarI CTCTTCn'nnn_
Cuts at: 1310 1520 1310
Size: 210 3077
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1495 1805 1951 3097 1495
Size: 310 146 1146 1685
Fragments arranged by size:
1685 1146 310 146
EcoICRI GAG'CTC
Cuts at: 299 299
Size: 3287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1112 1544 2291 2559 1112
Size: 432 747 268 1840
Fragments arranged by size:
1840 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 426 426
Size: 3287
EcoRI G'AATT_C
Cuts at: 291 291
Size: 3287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1306 1306
Size: 3287
FspI TGC'GCA
Cuts at: 1068 1068
Size: 3287
HaeII r_GCGC'y
Cuts at: 721 969 1991 2970 721
Size: 248 1022 979 1038
Fragments arranged by size:
1038 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1129 1159 1161 1191 1129
Size: 30 2 30 3225
Fragments arranged by size:
3225 30 30 2
HincII GTy'rAC
Cuts at: 326 326
Size: 3287
KasI G'GCGC_C
Cuts at: 717 965 717
Size: 248 3039
KpnI G_GTAC'C
Cuts at: 307 307
Size: 3287
MluI A'CGCG_T
Cuts at: 2720 2720
Size: 3287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1958 2142 1958
Size: 184 3103
MscI TGG'CCA
Cuts at: 1048 1048
Size: 3287
MslI CAynn'nnrTG
Cuts at: 1276 1403 1276
Size: 127 3160
MspA1I CmG'CkG
Cuts at: 398 712 1072 2085 2330 398
Size: 314 360 1013 245 1355
Fragments arranged by size:
1355 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1468 1468
Size: 3287
NarI GG'CG_CC
Cuts at: 718 966 718
Size: 248 3039
NcoI C'CATG_G
Cuts at: 107 107
Size: 3287
NgoMIV G'CCGG_C
Cuts at: 1466 1466
Size: 3287
NspI r_CATG'y
Cuts at: 340 840 1747 340
Size: 500 907 1880
Fragments arranged by size:
1880 907 500
PciI A'CATG_T
Cuts at: 1743 1743
Size: 3287
PmeI GTTT'AAAC
Cuts at: 286 286
Size: 3287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2426 2458 2426
Size: 32 3255
PspXI vC'TCGA_Gb
Cuts at: 278 278
Size: 3287
PstI C_TGCA'G
Cuts at: 334 1019 334
Size: 685 2602
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3287
PvuII CAG'CTG
Cuts at: 712 1072 712
Size: 360 2927
RsrII CG'GwC_CG
Cuts at: 1482 1482
Size: 3287
SacI G_AGCT'C
Cuts at: 301 301
Size: 3287
SalI G'TCGA_C
Cuts at: 324 324
Size: 3287
SapI GCTCTTCn'nnn_
Cuts at: 1310 1520 1310
Size: 210 3077
SbfI CC_TGCA'GG
Cuts at: 334 334
Size: 3287
ScaI AGT'ACT
Cuts at: 2804 2804
Size: 3287
SfcI C'TryA_G
Cuts at: 33 330 1015 2008 2199 33
Size: 297 685 993 191 1121
Fragments arranged by size:
1121 993 685 297 191
SfoI GGC'GCC
Cuts at: 719 967 719
Size: 248 3039
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3287
SmaI CCC'GGG
Cuts at: 309 309
Size: 3287
SmlI C'TyrA_G
Cuts at: 278 442 1357 1849 2111 278
Size: 164 915 492 262 1454
Fragments arranged by size:
1454 915 492 262 164
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3287
SphI G_CATG'C
Cuts at: 340 840 340
Size: 500 2787
StyI C'CwwG_G
Cuts at: 107 421 775 107
Size: 314 354 2619
Fragments arranged by size:
2619 354 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 870 1003 1462 1671 130
Size: 740 133 459 209 1746
Fragments arranged by size:
1746 740 459 209 133
TatI w'GTAC_w
Cuts at: 239 2802 239
Size: 2563 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 3255
Tth111I GACn'n_nGTC
Cuts at: 1084 1084
Size: 3287
XbaI T'CTAG_A
Cuts at: 318 318
Size: 3287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 782 782
Size: 3287
XhoI C'TCGA_G
Cuts at: 278 278
Size: 3287
XmaI C'CCGG_G
Cuts at: 307 307
Size: 3287
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI