Restriction map for
pF1KB7502
(Circular) (Six-base) MAPSORT of: pF1KB7502.seq Check: 6970 from: 1 to: 3821
pF1KB7502 3821 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 859 859
Size: 3821
Acc65I G'GTAC_C
Cuts at: 837 837
Size: 3821
AclI AA'CG_TT
Cuts at: 3661 3661
Size: 3821
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1646 2078 2825 3093 1646
Size: 432 747 268 2374
Fragments arranged by size:
2374 747 432 268
AflIII A'CryG_T
Cuts at: 1173 2277 3254 1173
Size: 1104 977 1740
Fragments arranged by size:
1740 1104 977
AgeI A'CCGG_T
Cuts at: 2209 2209
Size: 3821
AlwNI CAG_nnn'CTG
Cuts at: 1126 2693 1126
Size: 1567 2254
ApaLI G'TGCA_C
Cuts at: 2591 2591
Size: 3821
ApoI r'AATT_y
Cuts at: 825 3174 3430 825
Size: 2349 256 1216
Fragments arranged by size:
2349 1216 256
AseI AT'TA_AT
Cuts at: 20 635 20
Size: 615 3206
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3821
AvaI C'yCGr_G
Cuts at: 841 841
Size: 3821
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 124 157 124
Size: 33 3788
BamHI G'GATC_C
Cuts at: 846 846
Size: 3821
BanI G'GyrC_C
Cuts at: 462 837 1251 1499 1534 462
Size: 375 414 248 35 2749
Fragments arranged by size:
2749 414 375 248 35
BanII G_rGCy'C
Cuts at: 835 1865 835
Size: 1030 2791
BbeI G_GCGC'C
Cuts at: 1255 1503 1255
Size: 248 3573
BbsI GAAGACnn'nnnn_
Cuts at: 286 286
Size: 3821
BbvCI CC'TCA_GC
Cuts at: 611 611
Size: 3821
BciVI GTATCCnnnnn_n'
Cuts at: 1719 2480 3821 1719
Size: 761 1341 1719
Fragments arranged by size:
1719 1341 761
BclI T'GATC_A
Cuts at: 498 1341 498
Size: 843 2978
BglII A'GATC_T
Cuts at: 1336 3373 1336
Size: 2037 1784
BlpI GC'TnA_GC
Cuts at: 933 933
Size: 3821
Bme1580I G_kGCm'C
Cuts at: 1446 1539 2595 1446
Size: 93 1056 2672
Fragments arranged by size:
2672 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1033 1114 1449 3593 1033
Size: 81 335 2144 1261
Fragments arranged by size:
2144 1261 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 3821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 491
Size: 3821
Bpu10I CC'TnA_GC
Cuts at: 611 611
Size: 3821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1876 2368 2666 997
Size: 879 492 298 2152
Fragments arranged by size:
2152 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3525 3525
Size: 3821
BsaAI yAC'GTr
Cuts at: 1174 1174
Size: 3821
BsaBI GATnn'nnATC
Cuts at: 6 497 620 1335 3372 6
Size: 491 123 715 2037 455
Fragments arranged by size:
2037 715 491 455 123
BsaHI Gr'CG_yC
Cuts at: 112 1252 1500 2202 3698 112
Size: 1140 248 702 1496 235
Fragments arranged by size:
1496 1140 702 248 235
BsaWI w'CCGG_w
Cuts at: 1010 1531 2209 2483 2630 3041 1010
Size: 521 678 274 147 411 1790
Fragments arranged by size:
1790 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 302 332 464 494 302
Size: 30 132 30 3629
Fragments arranged by size:
3629 132 30 30
BseYI C'CCAG_C
Cuts at: 201 1246 2004 2581 3077 201
Size: 1045 758 577 496 945
Fragments arranged by size:
1045 945 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 630 630
Size: 3821
BsiEI CG_ry'CG
Cuts at: 104 1363 1409 2617 104
Size: 1259 46 1208 1308
Fragments arranged by size:
1308 1259 1208 46
BsiHKAI G_wGCw'C
Cuts at: 835 1613 1803 2595 835
Size: 778 190 792 2061
Fragments arranged by size:
2061 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 835 1446 1539 1613 1803 1865 2595 835
Size: 611 93 74 190 62 730 2061
Fragments arranged by size:
2061 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3041 3041
Size: 3821
BspHI T'CATG_A
Cuts at: 249 564 2997 249
Size: 315 2433 1073
Fragments arranged by size:
2433 1073 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 661 871 1085 1387 1768 661
Size: 210 214 302 381 2714
Fragments arranged by size:
2714 381 302 214 210
BsrBI CCG'CTC
Cuts at: 50 2113 2167 3651 3816 50
Size: 2063 54 1484 165 55
Fragments arranged by size:
2063 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1733 1733
Size: 3821
BsrFI r'CCGG_y
Cuts at: 1819 2000 2209 1819
Size: 181 209 3431
Fragments arranged by size:
3431 209 181
BssSI C'ACGA_G
Cuts at: 2092 2450 2092
Size: 358 3463
BstAPI GCAn_nnn'nTGC
Cuts at: 1198 1198
Size: 3821
BstBI TT'CG_AA
Cuts at: 2182 2182
Size: 3821
BstYI r'GATC_y
Cuts at: 1 846 1313 1336 1671 1917 2929 3015 3027
Size: 845 467 23 335 246 1012 86 12
Cuts at: 3027 3373 1
Size: 346 449
Fragments arranged by size:
1012 845 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 655 107
Size: 548 3273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1771 1952 2115 1771
Size: 181 163 3477
Fragments arranged by size:
3477 181 163
BtsI GCAGTG_nn'
Cuts at: 330 1064 330
Size: 734 3087
DraI TTT'AAA
Cuts at: 820 820
Size: 3821
DrdI GACnn_nn'nnGTC
Cuts at: 1527 2385 1527
Size: 858 2963
EaeI y'GGCC_r
Cuts at: 1406 1580 1971 1998 3343 1406
Size: 174 391 27 1345 1884
Fragments arranged by size:
1884 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1406 1406
Size: 3821
EarI CTCTTCn'nnn_
Cuts at: 269 718 1844 2054 269
Size: 449 1126 210 2036
Fragments arranged by size:
2036 1126 449 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2029 2339 2485 3631 2029
Size: 310 146 1146 2219
Fragments arranged by size:
2219 1146 310 146
EcoICRI GAG'CTC
Cuts at: 833 833
Size: 3821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1646 2078 2825 3093 491
Size: 1155 432 747 268 1219
Fragments arranged by size:
1219 1155 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 610 610
Size: 3821
EcoO109I rG'GnC_Cy
Cuts at: 960 960
Size: 3821
EcoRI G'AATT_C
Cuts at: 825 825
Size: 3821
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 747 1840 747
Size: 1093 2728
FspI TGC'GCA
Cuts at: 1602 1602
Size: 3821
HaeII r_GCGC'y
Cuts at: 579 1255 1503 2525 3504 579
Size: 676 248 1022 979 896
Fragments arranged by size:
1022 979 896 676 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1663 1693 1695 1725 1663
Size: 30 2 30 3759
Fragments arranged by size:
3759 30 30 2
HincII GTy'rAC
Cuts at: 860 860
Size: 3821
KasI G'GCGC_C
Cuts at: 1251 1499 1251
Size: 248 3573
KpnI G_GTAC'C
Cuts at: 841 841
Size: 3821
MluI A'CGCG_T
Cuts at: 3254 3254
Size: 3821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 288 2492 2676 288
Size: 2204 184 1433
Fragments arranged by size:
2204 1433 184
MscI TGG'CCA
Cuts at: 1582 1582
Size: 3821
MslI CAynn'nnrTG
Cuts at: 1810 1937 1810
Size: 127 3694
MspA1I CmG'CkG
Cuts at: 932 1246 1606 2619 2864 932
Size: 314 360 1013 245 1889
Fragments arranged by size:
1889 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2002 2002
Size: 3821
NarI GG'CG_CC
Cuts at: 1252 1500 1252
Size: 248 3573
NcoI C'CATG_G
Cuts at: 107 107
Size: 3821
NgoMIV G'CCGG_C
Cuts at: 2000 2000
Size: 3821
NspI r_CATG'y
Cuts at: 874 1374 2281 874
Size: 500 907 2414
Fragments arranged by size:
2414 907 500
PciI A'CATG_T
Cuts at: 2277 2277
Size: 3821
PmeI GTTT'AAAC
Cuts at: 820 820
Size: 3821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 3789
PstI C_TGCA'G
Cuts at: 672 868 1553 672
Size: 196 685 2940
Fragments arranged by size:
2940 685 196
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3821
PvuII CAG'CTG
Cuts at: 1246 1606 1246
Size: 360 3461
RsrII CG'GwC_CG
Cuts at: 2016 2016
Size: 3821
SacI G_AGCT'C
Cuts at: 835 835
Size: 3821
SalI G'TCGA_C
Cuts at: 858 858
Size: 3821
SapI GCTCTTCn'nnn_
Cuts at: 1844 2054 1844
Size: 210 3611
SbfI CC_TGCA'GG
Cuts at: 868 868
Size: 3821
ScaI AGT'ACT
Cuts at: 3338 3338
Size: 3821
SfcI C'TryA_G
Cuts at: 33 668 864 1549 2542 2733 33
Size: 635 196 685 993 191 1121
Fragments arranged by size:
1121 993 685 635 196 191
SfoI GGC'GCC
Cuts at: 1253 1501 1253
Size: 248 3573
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3821
SmaI CCC'GGG
Cuts at: 843 843
Size: 3821
SmlI C'TyrA_G
Cuts at: 976 1891 2383 2645 976
Size: 915 492 262 2152
Fragments arranged by size:
2152 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3821
SphI G_CATG'C
Cuts at: 874 1374 874
Size: 500 3321
StyI C'CwwG_G
Cuts at: 107 955 1309 107
Size: 848 354 2619
Fragments arranged by size:
2619 848 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1404 1537 1996 2205 1404
Size: 133 459 209 3020
Fragments arranged by size:
3020 459 209 133
TatI w'GTAC_w
Cuts at: 3336 3336
Size: 3821
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 302 334 464 496 3338 3370 302
Size: 32 130 32 2842 32 753
Fragments arranged by size:
2842 753 130 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1618 1618
Size: 3821
XbaI T'CTAG_A
Cuts at: 852 852
Size: 3821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1316 114
Size: 1202 2619
XmaI C'CCGG_G
Cuts at: 841 841
Size: 3821
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BcgI BfrBI BglI BmgBI BmtI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI