Restriction map for pF1KB7507
 (Circular) (Six-base) MAPSORT of: pF1KB7507.seq  Check: 5259  from: 1  to: 3440



pF1KB7507  3440 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AccI GT'mk_AC

Cuts at:    478     478 
   Size:       3440
 
Acc65I G'GTAC_C

Cuts at:    456     456 
   Size:       3440
 
AclI AA'CG_TT

Cuts at:   3280    3280 
   Size:       3440
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     205    1265    1697    2444    2712     136 
   Size:         69    1060     432     747     268     864

  Fragments arranged by size: 

                1060     864     747     432     268      69
 
AflIII A'CryG_T

Cuts at:    792    1896    2873     792 
   Size:       1104     977    1359

  Fragments arranged by size: 

                1359    1104     977
 
AgeI A'CCGG_T

Cuts at:   1828    1828 
   Size:       3440
 
AlwNI CAG_nnn'CTG

Cuts at:    308     745    2312     308 
   Size:        437    1567    1436

  Fragments arranged by size: 

                1567    1436     437
 
ApaLI G'TGCA_C

Cuts at:   2210    2210 
   Size:       3440
 
ApoI r'AATT_y

Cuts at:    346     444    2793    3049     346 
   Size:         98    2349     256     737

  Fragments arranged by size: 

                2349     737     256      98
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3440
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3440
 
AvaI C'yCGr_G

Cuts at:    460     460 
   Size:       3440
 
BamHI G'GATC_C

Cuts at:    465     465 
   Size:       3440
 
BanI G'GyrC_C

Cuts at:    456     870    1118    1153     456 
   Size:        414     248      35    2743

  Fragments arranged by size: 

                2743     414     248      35
 
BanII G_rGCy'C

Cuts at:    173     289     454    1484     173 
   Size:        116     165    1030    2129

  Fragments arranged by size: 

                2129    1030     165     116
 
BbeI G_GCGC'C

Cuts at:    874    1122     874 
   Size:        248    3192
 
BbsI GAAGACnn'nnnn_

Cuts at:    142     142 
   Size:       3440
 
BciVI GTATCCnnnnn_n'

Cuts at:   1338    2099    3440    1338 
   Size:        761    1341    1338

  Fragments arranged by size: 

                1341    1338     761
 
BclI T'GATC_A

Cuts at:    960     960 
   Size:       3440
 
BglII A'GATC_T

Cuts at:    955    2992     955 
   Size:       2037    1403
 
BlpI GC'TnA_GC

Cuts at:    552     552 
   Size:       3440
 
Bme1580I G_kGCm'C

Cuts at:   1065    1158    2214    1065 
   Size:         93    1056    2291

  Fragments arranged by size: 

                2291    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    652     733    1068    3212     652 
   Size:         81     335    2144     880

  Fragments arranged by size: 

                2144     880     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1807    1807 
   Size:       3440
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    616    1495    1987    2285     616 
   Size:        879     492     298    1771

  Fragments arranged by size: 

                1771     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3144    3144 
   Size:       3440
 
BsaAI yAC'GTr

Cuts at:    299     793     299 
   Size:        494    2946
 
BsaBI GATnn'nnATC

Cuts at:      6     954    2991       6 
   Size:        948    2037     455

  Fragments arranged by size: 

                2037     948     455
 
BsaHI Gr'CG_yC

Cuts at:    871    1119    1821    3317     871 
   Size:        248     702    1496     994

  Fragments arranged by size: 

                1496     994     702     248
 
BsaWI w'CCGG_w

Cuts at:    629    1150    1828    2102    2249    2660     629 
   Size:        521     678     274     147     411    1409

  Fragments arranged by size: 

                1409     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    865    1623    2200    2696     865 
   Size:        758     577     496    1609

  Fragments arranged by size: 

                1609     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     982    1028    2236     104 
   Size:        878      46    1208    1308

  Fragments arranged by size: 

                1308    1208     878      46
 
BsiHKAI G_wGCw'C

Cuts at:    173     454    1232    1422    2214     173 
   Size:        281     778     190     792    1399

  Fragments arranged by size: 

                1399     792     778     281     190
 
Bsp1286I G_dGCh'C

Cuts at:    173     289     454    1065    1158    1232    1422    1484    2214 
   Size:        116     165     611      93      74     190      62     730

Cuts at:   2214     173 
   Size:       1399

  Fragments arranged by size: 

                1399     730     611     190     165     116      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2660    2660 
   Size:       3440
 
BspHI T'CATG_A

Cuts at:   2616    2616 
   Size:       3440
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    490     704    1006    1387     490 
   Size:        214     302     381    2543

  Fragments arranged by size: 

                2543     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1732    1786    3270    3435      50 
   Size:       1682      54    1484     165      55

  Fragments arranged by size: 

                1682    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1352    1352 
   Size:       3440
 
BsrFI r'CCGG_y

Cuts at:   1438    1619    1828    1438 
   Size:        181     209    3050

  Fragments arranged by size: 

                3050     209     181
 
BssSI C'ACGA_G

Cuts at:   1711    2069    1711 
   Size:        358    3082
 
BstAPI GCAn_nnn'nTGC

Cuts at:    817     817 
   Size:       3440
 
BstBI TT'CG_AA

Cuts at:   1801    1801 
   Size:       3440
 
BstYI r'GATC_y

Cuts at:      1     465     932     955    1290    1536    2548    2634    2646 
   Size:        464     467      23     335     246    1012      86      12

Cuts at:   2646    2992       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     464     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3440
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1390    1571    1734    1390 
   Size:        181     163    3096

  Fragments arranged by size: 

                3096     181     163
 
BtsI GCAGTG_nn'

Cuts at:    683     683 
   Size:       3440
 
DraI TTT'AAA

Cuts at:    439     439 
   Size:       3440
 
DrdI GACnn_nn'nnGTC

Cuts at:   1146    2004    1146 
   Size:        858    2582
 
EaeI y'GGCC_r

Cuts at:   1025    1199    1590    1617    2962    1025 
   Size:        174     391      27    1345    1503

  Fragments arranged by size: 

                1503    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1025    1025 
   Size:       3440
 
EarI CTCTTCn'nnn_

Cuts at:   1463    1673    1463 
   Size:        210    3230
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1648    1958    2104    3250    1648 
   Size:        310     146    1146    1838

  Fragments arranged by size: 

                1838    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    171     452     171 
   Size:        281    3159
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     205    1265    1697    2444    2712     136 
   Size:         69    1060     432     747     268     864

  Fragments arranged by size: 

                1060     864     747     432     268      69
 
EcoO109I rG'GnC_Cy

Cuts at:    579     579 
   Size:       3440
 
EcoRI G'AATT_C

Cuts at:    346     444     346 
   Size:         98    3342
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    235    1459     235 
   Size:       1224    2216
 
FspI TGC'GCA

Cuts at:   1221    1221 
   Size:       3440
 
HaeII r_GCGC'y

Cuts at:    874    1122    2144    3123     874 
   Size:        248    1022     979    1191

  Fragments arranged by size: 

                1191    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1282    1312    1314    1344    1282 
   Size:         30       2      30    3378

  Fragments arranged by size: 

                3378      30      30       2
 
HincII GTy'rAC

Cuts at:    479     479 
   Size:       3440
 
KasI G'GCGC_C

Cuts at:    870    1118     870 
   Size:        248    3192
 
KpnI G_GTAC'C

Cuts at:    460     460 
   Size:       3440
 
MluI A'CGCG_T

Cuts at:   2873    2873 
   Size:       3440
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2111    2295    2111 
   Size:        184    3256
 
MscI TGG'CCA

Cuts at:   1201    1201 
   Size:       3440
 
MslI CAynn'nnrTG

Cuts at:   1429    1556    1429 
   Size:        127    3313
 
MspA1I CmG'CkG

Cuts at:    551     865    1225    2238    2483     551 
   Size:        314     360    1013     245    1508

  Fragments arranged by size: 

                1508    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1621    1621 
   Size:       3440
 
NarI GG'CG_CC

Cuts at:    871    1119     871 
   Size:        248    3192
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3440
 
NgoMIV G'CCGG_C

Cuts at:   1619    1619 
   Size:       3440
 
NspI r_CATG'y

Cuts at:    493     993    1900     493 
   Size:        500     907    2033

  Fragments arranged by size: 

                2033     907     500
 
PciI A'CATG_T

Cuts at:   1896    1896 
   Size:       3440
 
PmeI GTTT'AAAC

Cuts at:    439     439 
   Size:       3440
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2579    2611    2579 
   Size:         32    3408
 
PstI C_TGCA'G

Cuts at:    487    1172     487 
   Size:        685    2755
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3440
 
PvuII CAG'CTG

Cuts at:    865    1225     865 
   Size:        360    3080
 
RsrII CG'GwC_CG

Cuts at:   1635    1635 
   Size:       3440
 
SacI G_AGCT'C

Cuts at:    173     454     173 
   Size:        281    3159
 
SalI G'TCGA_C

Cuts at:    477     477 
   Size:       3440
 
SapI GCTCTTCn'nnn_

Cuts at:   1463    1673    1463 
   Size:        210    3230
 
SbfI CC_TGCA'GG

Cuts at:    487     487 
   Size:       3440
 
ScaI AGT'ACT

Cuts at:   2957    2957 
   Size:       3440
 
SfcI C'TryA_G

Cuts at:     33     428     483    1168    2161    2352      33 
   Size:        395      55     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     395     191      55
 
SfoI GGC'GCC

Cuts at:    872    1120     872 
   Size:        248    3192
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3440
 
SmaI CCC'GGG

Cuts at:    462     462 
   Size:       3440
 
SmlI C'TyrA_G

Cuts at:    595    1510    2002    2264     595 
   Size:        915     492     262    1771

  Fragments arranged by size: 

                1771     915     492     262
 
SnaBI TAC'GTA

Cuts at:    299     299 
   Size:       3440
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3440
 
SphI G_CATG'C

Cuts at:    493     993     493 
   Size:        500    2940
 
StyI C'CwwG_G

Cuts at:    107     574     928     107 
   Size:        467     354    2619

  Fragments arranged by size: 

                2619     467     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1023    1156    1615    1824    1023 
   Size:        133     459     209    2639

  Fragments arranged by size: 

                2639     459     209     133
 
TatI w'GTAC_w

Cuts at:    330    2955     330 
   Size:       2625     815
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2957    2989    2957 
   Size:         32    3408
 
Tth111I GACn'n_nGTC

Cuts at:   1237    1237 
   Size:       3440
 
XbaI T'CTAG_A

Cuts at:    471     471 
   Size:       3440
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    935     935 
   Size:       3440
 
XmaI C'CCGG_G

Cuts at:    460     460 
   Size:       3440
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI
     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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