(Circular) (Six-base) MAPSORT of: pF1KB7507.seq Check: 5259 from: 1 to: 3440 pF1KB7507 3440 bp With 182 enzymes: SgfI * June 20, 2008 10:27 .. AccI GT'mk_AC Cuts at: 478 478 Size: 3440 Acc65I G'GTAC_C Cuts at: 456 456 Size: 3440 AclI AA'CG_TT Cuts at: 3280 3280 Size: 3440 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 136 205 1265 1697 2444 2712 136 Size: 69 1060 432 747 268 864 Fragments arranged by size: 1060 864 747 432 268 69 AflIII A'CryG_T Cuts at: 792 1896 2873 792 Size: 1104 977 1359 Fragments arranged by size: 1359 1104 977 AgeI A'CCGG_T Cuts at: 1828 1828 Size: 3440 AlwNI CAG_nnn'CTG Cuts at: 308 745 2312 308 Size: 437 1567 1436 Fragments arranged by size: 1567 1436 437 ApaLI G'TGCA_C Cuts at: 2210 2210 Size: 3440 ApoI r'AATT_y Cuts at: 346 444 2793 3049 346 Size: 98 2349 256 737 Fragments arranged by size: 2349 737 256 98 AseI AT'TA_AT Cuts at: 20 20 Size: 3440 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3440 AvaI C'yCGr_G Cuts at: 460 460 Size: 3440 BamHI G'GATC_C Cuts at: 465 465 Size: 3440 BanI G'GyrC_C Cuts at: 456 870 1118 1153 456 Size: 414 248 35 2743 Fragments arranged by size: 2743 414 248 35 BanII G_rGCy'C Cuts at: 173 289 454 1484 173 Size: 116 165 1030 2129 Fragments arranged by size: 2129 1030 165 116 BbeI G_GCGC'C Cuts at: 874 1122 874 Size: 248 3192 BbsI GAAGACnn'nnnn_ Cuts at: 142 142 Size: 3440 BciVI GTATCCnnnnn_n' Cuts at: 1338 2099 3440 1338 Size: 761 1341 1338 Fragments arranged by size: 1341 1338 761 BclI T'GATC_A Cuts at: 960 960 Size: 3440 BglII A'GATC_T Cuts at: 955 2992 955 Size: 2037 1403 BlpI GC'TnA_GC Cuts at: 552 552 Size: 3440 Bme1580I G_kGCm'C Cuts at: 1065 1158 2214 1065 Size: 93 1056 2291 Fragments arranged by size: 2291 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 652 733 1068 3212 652 Size: 81 335 2144 880 Fragments arranged by size: 2144 880 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1807 1807 Size: 3440 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 616 1495 1987 2285 616 Size: 879 492 298 1771 Fragments arranged by size: 1771 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3144 3144 Size: 3440 BsaAI yAC'GTr Cuts at: 299 793 299 Size: 494 2946 BsaBI GATnn'nnATC Cuts at: 6 954 2991 6 Size: 948 2037 455 Fragments arranged by size: 2037 948 455 BsaHI Gr'CG_yC Cuts at: 871 1119 1821 3317 871 Size: 248 702 1496 994 Fragments arranged by size: 1496 994 702 248 BsaWI w'CCGG_w Cuts at: 629 1150 1828 2102 2249 2660 629 Size: 521 678 274 147 411 1409 Fragments arranged by size: 1409 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 865 1623 2200 2696 865 Size: 758 577 496 1609 Fragments arranged by size: 1609 758 577 496 BsiEI CG_ry'CG Cuts at: 104 982 1028 2236 104 Size: 878 46 1208 1308 Fragments arranged by size: 1308 1208 878 46 BsiHKAI G_wGCw'C Cuts at: 173 454 1232 1422 2214 173 Size: 281 778 190 792 1399 Fragments arranged by size: 1399 792 778 281 190 Bsp1286I G_dGCh'C Cuts at: 173 289 454 1065 1158 1232 1422 1484 2214 Size: 116 165 611 93 74 190 62 730 Cuts at: 2214 173 Size: 1399 Fragments arranged by size: 1399 730 611 190 165 116 93 74 62 BspEI T'CCGG_A Cuts at: 2660 2660 Size: 3440 BspHI T'CATG_A Cuts at: 2616 2616 Size: 3440 BspMI ACCTGCnnnn'nnnn_ Cuts at: 490 704 1006 1387 490 Size: 214 302 381 2543 Fragments arranged by size: 2543 381 302 214 BsrBI CCG'CTC Cuts at: 50 1732 1786 3270 3435 50 Size: 1682 54 1484 165 55 Fragments arranged by size: 1682 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1352 1352 Size: 3440 BsrFI r'CCGG_y Cuts at: 1438 1619 1828 1438 Size: 181 209 3050 Fragments arranged by size: 3050 209 181 BssSI C'ACGA_G Cuts at: 1711 2069 1711 Size: 358 3082 BstAPI GCAn_nnn'nTGC Cuts at: 817 817 Size: 3440 BstBI TT'CG_AA Cuts at: 1801 1801 Size: 3440 BstYI r'GATC_y Cuts at: 1 465 932 955 1290 1536 2548 2634 2646 Size: 464 467 23 335 246 1012 86 12 Cuts at: 2646 2992 1 Size: 346 449 Fragments arranged by size: 1012 467 464 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3440 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1390 1571 1734 1390 Size: 181 163 3096 Fragments arranged by size: 3096 181 163 BtsI GCAGTG_nn' Cuts at: 683 683 Size: 3440 DraI TTT'AAA Cuts at: 439 439 Size: 3440 DrdI GACnn_nn'nnGTC Cuts at: 1146 2004 1146 Size: 858 2582 EaeI y'GGCC_r Cuts at: 1025 1199 1590 1617 2962 1025 Size: 174 391 27 1345 1503 Fragments arranged by size: 1503 1345 391 174 27 EagI C'GGCC_G Cuts at: 1025 1025 Size: 3440 EarI CTCTTCn'nnn_ Cuts at: 1463 1673 1463 Size: 210 3230 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1648 1958 2104 3250 1648 Size: 310 146 1146 1838 Fragments arranged by size: 1838 1146 310 146 EcoICRI GAG'CTC Cuts at: 171 452 171 Size: 281 3159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 136 205 1265 1697 2444 2712 136 Size: 69 1060 432 747 268 864 Fragments arranged by size: 1060 864 747 432 268 69 EcoO109I rG'GnC_Cy Cuts at: 579 579 Size: 3440 EcoRI G'AATT_C Cuts at: 346 444 346 Size: 98 3342 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 235 1459 235 Size: 1224 2216 FspI TGC'GCA Cuts at: 1221 1221 Size: 3440 HaeII r_GCGC'y Cuts at: 874 1122 2144 3123 874 Size: 248 1022 979 1191 Fragments arranged by size: 1191 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1282 1312 1314 1344 1282 Size: 30 2 30 3378 Fragments arranged by size: 3378 30 30 2 HincII GTy'rAC Cuts at: 479 479 Size: 3440 KasI G'GCGC_C Cuts at: 870 1118 870 Size: 248 3192 KpnI G_GTAC'C Cuts at: 460 460 Size: 3440 MluI A'CGCG_T Cuts at: 2873 2873 Size: 3440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2111 2295 2111 Size: 184 3256 MscI TGG'CCA Cuts at: 1201 1201 Size: 3440 MslI CAynn'nnrTG Cuts at: 1429 1556 1429 Size: 127 3313 MspA1I CmG'CkG Cuts at: 551 865 1225 2238 2483 551 Size: 314 360 1013 245 1508 Fragments arranged by size: 1508 1013 360 314 245 NaeI GCC'GGC Cuts at: 1621 1621 Size: 3440 NarI GG'CG_CC Cuts at: 871 1119 871 Size: 248 3192 NcoI C'CATG_G Cuts at: 107 107 Size: 3440 NgoMIV G'CCGG_C Cuts at: 1619 1619 Size: 3440 NspI r_CATG'y Cuts at: 493 993 1900 493 Size: 500 907 2033 Fragments arranged by size: 2033 907 500 PciI A'CATG_T Cuts at: 1896 1896 Size: 3440 PmeI GTTT'AAAC Cuts at: 439 439 Size: 3440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2579 2611 2579 Size: 32 3408 PstI C_TGCA'G Cuts at: 487 1172 487 Size: 685 2755 PvuI CG_AT'CG Cuts at: 104 104 Size: 3440 PvuII CAG'CTG Cuts at: 865 1225 865 Size: 360 3080 RsrII CG'GwC_CG Cuts at: 1635 1635 Size: 3440 SacI G_AGCT'C Cuts at: 173 454 173 Size: 281 3159 SalI G'TCGA_C Cuts at: 477 477 Size: 3440 SapI GCTCTTCn'nnn_ Cuts at: 1463 1673 1463 Size: 210 3230 SbfI CC_TGCA'GG Cuts at: 487 487 Size: 3440 ScaI AGT'ACT Cuts at: 2957 2957 Size: 3440 SfcI C'TryA_G Cuts at: 33 428 483 1168 2161 2352 33 Size: 395 55 685 993 191 1121 Fragments arranged by size: 1121 993 685 395 191 55 SfoI GGC'GCC Cuts at: 872 1120 872 Size: 248 3192 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3440 SmaI CCC'GGG Cuts at: 462 462 Size: 3440 SmlI C'TyrA_G Cuts at: 595 1510 2002 2264 595 Size: 915 492 262 1771 Fragments arranged by size: 1771 915 492 262 SnaBI TAC'GTA Cuts at: 299 299 Size: 3440 SpeI A'CTAG_T Cuts at: 67 67 Size: 3440 SphI G_CATG'C Cuts at: 493 993 493 Size: 500 2940 StyI C'CwwG_G Cuts at: 107 574 928 107 Size: 467 354 2619 Fragments arranged by size: 2619 467 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1023 1156 1615 1824 1023 Size: 133 459 209 2639 Fragments arranged by size: 2639 459 209 133 TatI w'GTAC_w Cuts at: 330 2955 330 Size: 2625 815 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2957 2989 2957 Size: 32 3408 Tth111I GACn'n_nGTC Cuts at: 1237 1237 Size: 3440 XbaI T'CTAG_A Cuts at: 471 471 Size: 3440 XcmI CCAnnnn_n'nnnnTGG Cuts at: 935 935 Size: 3440 XmaI C'CCGG_G Cuts at: 460 460 Size: 3440 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI