Restriction map for
pF1KB7509
(Circular) (Six-base) MAPSORT of: pF1KB7509.seq Check: 8118 from: 1 to: 3449
pF1KB7509 3449 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 487 487
Size: 3449
Acc65I G'GTAC_C
Cuts at: 465 465
Size: 3449
AclI AA'CG_TT
Cuts at: 3289 3289
Size: 3449
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1274 1706 2453 2721 1274
Size: 432 747 268 2002
Fragments arranged by size:
2002 747 432 268
AflIII A'CryG_T
Cuts at: 309 801 1905 2882 309
Size: 492 1104 977 876
Fragments arranged by size:
1104 977 876 492
AgeI A'CCGG_T
Cuts at: 1837 1837
Size: 3449
AlwNI CAG_nnn'CTG
Cuts at: 754 2321 754
Size: 1567 1882
ApaLI G'TGCA_C
Cuts at: 2219 2219
Size: 3449
ApoI r'AATT_y
Cuts at: 190 382 453 2802 3058 190
Size: 192 71 2349 256 581
Fragments arranged by size:
2349 581 256 192 71
AseI AT'TA_AT
Cuts at: 20 20
Size: 3449
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3449
AvaI C'yCGr_G
Cuts at: 469 469
Size: 3449
BamHI G'GATC_C
Cuts at: 474 474
Size: 3449
BanI G'GyrC_C
Cuts at: 465 879 1127 1162 465
Size: 414 248 35 2752
Fragments arranged by size:
2752 414 248 35
BanII G_rGCy'C
Cuts at: 463 1493 463
Size: 1030 2419
BbeI G_GCGC'C
Cuts at: 883 1131 883
Size: 248 3201
BciVI GTATCCnnnnn_n'
Cuts at: 1347 2108 3449 1347
Size: 761 1341 1347
Fragments arranged by size:
1347 1341 761
BclI T'GATC_A
Cuts at: 969 969
Size: 3449
BfrBI ATG'CAT
Cuts at: 214 329 214
Size: 115 3334
BglII A'GATC_T
Cuts at: 964 3001 964
Size: 2037 1412
BlpI GC'TnA_GC
Cuts at: 561 561
Size: 3449
Bme1580I G_kGCm'C
Cuts at: 1074 1167 2223 1074
Size: 93 1056 2300
Fragments arranged by size:
2300 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 661 742 1077 3221 661
Size: 81 335 2144 889
Fragments arranged by size:
2144 889 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1816 1816
Size: 3449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1504 1996 2294 625
Size: 879 492 298 1780
Fragments arranged by size:
1780 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3153 3153
Size: 3449
BsaAI yAC'GTr
Cuts at: 341 802 341
Size: 461 2988
BsaBI GATnn'nnATC
Cuts at: 6 963 3000 6
Size: 957 2037 455
Fragments arranged by size:
2037 957 455
BsaHI Gr'CG_yC
Cuts at: 880 1128 1830 3326 880
Size: 248 702 1496 1003
Fragments arranged by size:
1496 1003 702 248
BsaWI w'CCGG_w
Cuts at: 638 1159 1837 2111 2258 2669 638
Size: 521 678 274 147 411 1418
Fragments arranged by size:
1418 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 133
Size: 3449
BseYI C'CCAG_C
Cuts at: 874 1632 2209 2705 874
Size: 758 577 496 1618
Fragments arranged by size:
1618 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 991 1037 2245 104
Size: 887 46 1208 1308
Fragments arranged by size:
1308 1208 887 46
BsiHKAI G_wGCw'C
Cuts at: 463 1241 1431 2223 463
Size: 778 190 792 1689
Fragments arranged by size:
1689 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 463 1074 1167 1241 1431 1493 2223 463
Size: 611 93 74 190 62 730 1689
Fragments arranged by size:
1689 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2669 2669
Size: 3449
BspHI T'CATG_A
Cuts at: 2625 2625
Size: 3449
BspMI ACCTGCnnnn'nnnn_
Cuts at: 499 713 1015 1396 499
Size: 214 302 381 2552
Fragments arranged by size:
2552 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1741 1795 3279 3444 50
Size: 1691 54 1484 165 55
Fragments arranged by size:
1691 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1361 1361
Size: 3449
BsrFI r'CCGG_y
Cuts at: 1447 1628 1837 1447
Size: 181 209 3059
Fragments arranged by size:
3059 209 181
BssSI C'ACGA_G
Cuts at: 1720 2078 1720
Size: 358 3091
BstAPI GCAn_nnn'nTGC
Cuts at: 826 826
Size: 3449
BstBI TT'CG_AA
Cuts at: 1810 1810
Size: 3449
BstYI r'GATC_y
Cuts at: 1 474 941 964 1299 1545 2557 2643 2655
Size: 473 467 23 335 246 1012 86 12
Cuts at: 2655 3001 1
Size: 346 449
Fragments arranged by size:
1012 473 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3449
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1399 1580 1743 1399
Size: 181 163 3105
Fragments arranged by size:
3105 181 163
BtsI GCAGTG_nn'
Cuts at: 692 692
Size: 3449
DraI TTT'AAA
Cuts at: 448 448
Size: 3449
DrdI GACnn_nn'nnGTC
Cuts at: 1155 2013 1155
Size: 858 2591
EaeI y'GGCC_r
Cuts at: 183 1034 1208 1599 1626 2971 183
Size: 851 174 391 27 1345 661
Fragments arranged by size:
1345 851 661 391 174 27
EagI C'GGCC_G
Cuts at: 1034 1034
Size: 3449
EarI CTCTTCn'nnn_
Cuts at: 1472 1682 1472
Size: 210 3239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1657 1967 2113 3259 1657
Size: 310 146 1146 1847
Fragments arranged by size:
1847 1146 310 146
EcoICRI GAG'CTC
Cuts at: 461 461
Size: 3449
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1274 1706 2453 2721 1274
Size: 432 747 268 2002
Fragments arranged by size:
2002 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 145 588 145
Size: 443 3006
EcoRI G'AATT_C
Cuts at: 382 453 382
Size: 71 3378
EcoRV GAT'ATC
Cuts at: 173 173
Size: 3449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1468 1468
Size: 3449
FspI TGC'GCA
Cuts at: 1230 1230
Size: 3449
HaeII r_GCGC'y
Cuts at: 883 1131 2153 3132 883
Size: 248 1022 979 1200
Fragments arranged by size:
1200 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1291 1321 1323 1353 1291
Size: 30 2 30 3387
Fragments arranged by size:
3387 30 30 2
HincII GTy'rAC
Cuts at: 488 488
Size: 3449
KasI G'GCGC_C
Cuts at: 879 1127 879
Size: 248 3201
KpnI G_GTAC'C
Cuts at: 469 469
Size: 3449
MfeI C'AATT_G
Cuts at: 389 389
Size: 3449
MluI A'CGCG_T
Cuts at: 2882 2882
Size: 3449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2120 2304 2120
Size: 184 3265
MscI TGG'CCA
Cuts at: 185 1210 185
Size: 1025 2424
MslI CAynn'nnrTG
Cuts at: 1438 1565 1438
Size: 127 3322
MspA1I CmG'CkG
Cuts at: 560 874 1234 2247 2492 560
Size: 314 360 1013 245 1517
Fragments arranged by size:
1517 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1630 1630
Size: 3449
NarI GG'CG_CC
Cuts at: 880 1128 880
Size: 248 3201
NcoI C'CATG_G
Cuts at: 107 107
Size: 3449
NgoMIV G'CCGG_C
Cuts at: 1628 1628
Size: 3449
NsiI A_TGCA'T
Cuts at: 216 331 216
Size: 115 3334
NspI r_CATG'y
Cuts at: 214 313 333 502 1002 1909 214
Size: 99 20 169 500 907 1754
Fragments arranged by size:
1754 907 500 169 99 20
PciI A'CATG_T
Cuts at: 309 1905 309
Size: 1596 1853
PmeI GTTT'AAAC
Cuts at: 448 448
Size: 3449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2588 2620 2588
Size: 32 3417
PpuMI rG'GwC_Cy
Cuts at: 145 145
Size: 3449
PstI C_TGCA'G
Cuts at: 496 1181 496
Size: 685 2764
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3449
PvuII CAG'CTG
Cuts at: 874 1234 874
Size: 360 3089
RsrII CG'GwC_CG
Cuts at: 1644 1644
Size: 3449
SacI G_AGCT'C
Cuts at: 463 463
Size: 3449
SalI G'TCGA_C
Cuts at: 486 486
Size: 3449
SapI GCTCTTCn'nnn_
Cuts at: 1472 1682 1472
Size: 210 3239
SbfI CC_TGCA'GG
Cuts at: 496 496
Size: 3449
ScaI AGT'ACT
Cuts at: 2966 2966
Size: 3449
SfcI C'TryA_G
Cuts at: 33 492 1177 2170 2361 33
Size: 459 685 993 191 1121
Fragments arranged by size:
1121 993 685 459 191
SfoI GGC'GCC
Cuts at: 881 1129 881
Size: 248 3201
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3449
SmaI CCC'GGG
Cuts at: 471 471
Size: 3449
SmlI C'TyrA_G
Cuts at: 604 1519 2011 2273 604
Size: 915 492 262 1780
Fragments arranged by size:
1780 915 492 262
SnaBI TAC'GTA
Cuts at: 341 341
Size: 3449
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3449
SphI G_CATG'C
Cuts at: 502 1002 502
Size: 500 2949
StyI C'CwwG_G
Cuts at: 107 583 937 107
Size: 476 354 2619
Fragments arranged by size:
2619 476 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1032 1165 1624 1833 1032
Size: 133 459 209 2648
Fragments arranged by size:
2648 459 209 133
TatI w'GTAC_w
Cuts at: 2964 2964
Size: 3449
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 360 392 2966 2998 360
Size: 32 2574 32 811
Fragments arranged by size:
2574 811 32 32
Tth111I GACn'n_nGTC
Cuts at: 1246 1246
Size: 3449
XbaI T'CTAG_A
Cuts at: 480 480
Size: 3449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 944 944
Size: 3449
XmaI C'CCGG_G
Cuts at: 469 469
Size: 3449
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI
ZraI