Restriction map for
pF1KB7512
(Circular) (Six-base) MAPSORT of: pF1KB7512.seq Check: 512 from: 1 to: 3842
pF1KB7512 3842 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1667 2099 2846 3114 1667
Size: 432 747 268 2395
Fragments arranged by size:
2395 747 432 268
AflIII A'CryG_T
Cuts at: 1194 2298 3275 1194
Size: 1104 977 1761
Fragments arranged by size:
1761 1104 977
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AleI CACnn'nnGTG
Cuts at: 419 419
Size: 3842
AlwNI CAG_nnn'CTG
Cuts at: 310 640 771 1147 2714 310
Size: 330 131 376 1567 1438
Fragments arranged by size:
1567 1438 376 330 131
ApaLI G'TGCA_C
Cuts at: 2612 2612
Size: 3842
ApoI r'AATT_y
Cuts at: 846 3195 3451 846
Size: 2349 256 1237
Fragments arranged by size:
2349 1237 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 386 862 386
Size: 476 3366
BamHI G'GATC_C
Cuts at: 867 867
Size: 3842
BanI G'GyrC_C
Cuts at: 776 858 1272 1520 1555 776
Size: 82 414 248 35 3063
Fragments arranged by size:
3063 414 248 82 35
BanII G_rGCy'C
Cuts at: 856 1886 856
Size: 1030 2812
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BbvCI CC'TCA_GC
Cuts at: 586 586
Size: 3842
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2501 3842 1740
Size: 761 1341 1740
Fragments arranged by size:
1740 1341 761
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 954 954
Size: 3842
Bme1580I G_kGCm'C
Cuts at: 1467 1560 2616 1467
Size: 93 1056 2693
Fragments arranged by size:
2693 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 628 1054 1135 1470 3614 628
Size: 426 81 335 2144 856
Fragments arranged by size:
2144 856 426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 3842
Bpu10I CC'TnA_GC
Cuts at: 586 764 586
Size: 178 3664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1897 2389 2687 1018
Size: 879 492 298 2173
Fragments arranged by size:
2173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3546 3546
Size: 3842
BsaAI yAC'GTr
Cuts at: 1195 1195
Size: 3842
BsaBI GATnn'nnATC
Cuts at: 6 227 1356 3393 6
Size: 221 1129 2037 455
Fragments arranged by size:
2037 1129 455 221
BsaHI Gr'CG_yC
Cuts at: 1273 1521 2223 3719 1273
Size: 248 702 1496 1396
Fragments arranged by size:
1496 1396 702 248
BsaWI w'CCGG_w
Cuts at: 1031 1552 2230 2504 2651 3062 1031
Size: 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 453 523 526 541 453
Size: 70 3 15 3754
Fragments arranged by size:
3754 70 15 3
BseYI C'CCAG_C
Cuts at: 1267 2025 2602 3098 1267
Size: 758 577 496 2011
Fragments arranged by size:
2011 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1384 1430 2638 104
Size: 1280 46 1208 1308
Fragments arranged by size:
1308 1280 1208 46
BsiHKAI G_wGCw'C
Cuts at: 203 856 1634 1824 2616 203
Size: 653 778 190 792 1429
Fragments arranged by size:
1429 792 778 653 190
Bsp1286I G_dGCh'C
Cuts at: 203 856 1467 1560 1634 1824 1886 2616 203
Size: 653 611 93 74 190 62 730 1429
Fragments arranged by size:
1429 730 653 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 299 3018 299
Size: 2719 1123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 154 892 1106 1408 1789 154
Size: 738 214 302 381 2207
Fragments arranged by size:
2207 738 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2134 2188 3672 3837 50
Size: 2084 54 1484 165 55
Fragments arranged by size:
2084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1754 1754
Size: 3842
BsrFI r'CCGG_y
Cuts at: 1840 2021 2230 1840
Size: 181 209 3452
Fragments arranged by size:
3452 209 181
BssSI C'ACGA_G
Cuts at: 2113 2471 2113
Size: 358 3484
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 1219
Size: 3842
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstXI CCAn_nnnn'nTGG
Cuts at: 421 421
Size: 3842
BstYI r'GATC_y
Cuts at: 1 228 867 1334 1357 1692 1938 2950 3036
Size: 227 639 467 23 335 246 1012 86
Cuts at: 3036 3048 3394 1
Size: 12 346 449
Fragments arranged by size:
1012 639 467 449 346 335 246 227
86 23 12
BtgI C'CryG_G
Cuts at: 232 232
Size: 3842
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 102 554 1792 1973 2136 102
Size: 452 1238 181 163 1808
Fragments arranged by size:
1808 1238 452 181 163
BtsI GCAGTG_nn'
Cuts at: 426 1085 426
Size: 659 3183
ClaI AT'CG_AT
Cuts at: 221 221
Size: 3842
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2406 1548
Size: 858 2984
EaeI y'GGCC_r
Cuts at: 185 1427 1601 1992 2019 3364 185
Size: 1242 174 391 27 1345 663
Fragments arranged by size:
1345 1242 663 391 174 27
EagI C'GGCC_G
Cuts at: 1427 1427
Size: 3842
EarI CTCTTCn'nnn_
Cuts at: 471 608 742 1865 2075 471
Size: 137 134 1123 210 2238
Fragments arranged by size:
2238 1123 210 137 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2050 2360 2506 3652 2050
Size: 310 146 1146 2240
Fragments arranged by size:
2240 1146 310 146
EcoICRI GAG'CTC
Cuts at: 854 854
Size: 3842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1667 2099 2846 3114 1667
Size: 432 747 268 2395
Fragments arranged by size:
2395 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 768 981 768
Size: 213 3629
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3842
FspI TGC'GCA
Cuts at: 1623 1623
Size: 3842
HaeII r_GCGC'y
Cuts at: 1276 1524 2546 3525 1276
Size: 248 1022 979 1593
Fragments arranged by size:
1593 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 1684 1714 1716 1746 313
Size: 32 1339 30 2 30 2409
Fragments arranged by size:
2409 1339 32 30 30 2
HincII GTy'rAC
Cuts at: 881 881
Size: 3842
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 511 512 2513 2697 511
Size: 1 2001 184 1656
Fragments arranged by size:
2001 1656 184 1
MscI TGG'CCA
Cuts at: 1603 1603
Size: 3842
MslI CAynn'nnrTG
Cuts at: 419 598 732 1831 1958 419
Size: 179 134 1099 127 2303
Fragments arranged by size:
2303 1099 179 134 127
MspA1I CmG'CkG
Cuts at: 953 1267 1627 2640 2885 953
Size: 314 360 1013 245 1910
Fragments arranged by size:
1910 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 3842
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 3842
NspI r_CATG'y
Cuts at: 895 1395 2302 895
Size: 500 907 2435
Fragments arranged by size:
2435 907 500
PciI A'CATG_T
Cuts at: 2298 2298
Size: 3842
PflMI CCAn_nnn'nTGG
Cuts at: 640 640
Size: 3842
PfoI T'CCnGG_A
Cuts at: 547 639 547
Size: 92 3750
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 3810
PstI C_TGCA'G
Cuts at: 889 1574 889
Size: 685 3157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 1267 1627 1267
Size: 360 3482
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 856 856
Size: 3842
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SfcI C'TryA_G
Cuts at: 33 885 1570 2563 2754 33
Size: 852 685 993 191 1121
Fragments arranged by size:
1121 993 852 685 191
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 386 997 1912 2404 2666 386
Size: 611 915 492 262 1562
Fragments arranged by size:
1562 915 611 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StyI C'CwwG_G
Cuts at: 976 1330 976
Size: 354 3488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 465 1425 1558 2017 2226 465
Size: 960 133 459 209 2081
Fragments arranged by size:
2081 960 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 3810
Tth111I GACn'n_nGTC
Cuts at: 369 1639 369
Size: 1270 2572
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1337 1337
Size: 3842
XhoI C'TCGA_G
Cuts at: 386 386
Size: 3842
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI