Restriction map for
pF1KB7513
(Circular) (Six-base) MAPSORT of: pF1KB7513.seq Check: 6352 from: 1 to: 3488
pF1KB7513 3488 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 526 526
Size: 3488
Acc65I G'GTAC_C
Cuts at: 504 504
Size: 3488
AclI AA'CG_TT
Cuts at: 3328 3328
Size: 3488
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1313 1745 2492 2760 1313
Size: 432 747 268 2041
Fragments arranged by size:
2041 747 432 268
AflIII A'CryG_T
Cuts at: 218 320 443 840 1944 2921 218
Size: 102 123 397 1104 977 785
Fragments arranged by size:
1104 977 785 397 123 102
AgeI A'CCGG_T
Cuts at: 1876 1876
Size: 3488
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 376 408 376
Size: 32 3456
AlwNI CAG_nnn'CTG
Cuts at: 793 2360 793
Size: 1567 1921
ApaLI G'TGCA_C
Cuts at: 447 2258 447
Size: 1811 1677
ApoI r'AATT_y
Cuts at: 492 2841 3097 492
Size: 2349 256 883
Fragments arranged by size:
2349 883 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3488
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3488
AvaI C'yCGr_G
Cuts at: 508 508
Size: 3488
BamHI G'GATC_C
Cuts at: 513 513
Size: 3488
BanI G'GyrC_C
Cuts at: 504 918 1166 1201 504
Size: 414 248 35 2791
Fragments arranged by size:
2791 414 248 35
BanII G_rGCy'C
Cuts at: 116 502 1532 116
Size: 386 1030 2072
Fragments arranged by size:
2072 1030 386
BbeI G_GCGC'C
Cuts at: 922 1170 922
Size: 248 3240
BbsI GAAGACnn'nnnn_
Cuts at: 197 197
Size: 3488
BciVI GTATCCnnnnn_n'
Cuts at: 1386 2147 3488 1386
Size: 761 1341 1386
Fragments arranged by size:
1386 1341 761
BclI T'GATC_A
Cuts at: 1008 1008
Size: 3488
BglII A'GATC_T
Cuts at: 1003 3040 1003
Size: 2037 1451
BlpI GC'TnA_GC
Cuts at: 600 600
Size: 3488
Bme1580I G_kGCm'C
Cuts at: 451 1113 1206 2262 451
Size: 662 93 1056 1677
Fragments arranged by size:
1677 1056 662 93
BmgBI CAC'GTC
Cuts at: 321 321
Size: 3488
BmrI ACTGGGnnnn_n'
Cuts at: 700 781 1116 3260 700
Size: 81 335 2144 928
Fragments arranged by size:
2144 928 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1543 2035 2333 664
Size: 879 492 298 1819
Fragments arranged by size:
1819 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3192 3192
Size: 3488
BsaAI yAC'GTr
Cuts at: 444 841 444
Size: 397 3091
BsaBI GATnn'nnATC
Cuts at: 6 1002 3039 6
Size: 996 2037 455
Fragments arranged by size:
2037 996 455
BsaHI Gr'CG_yC
Cuts at: 425 919 1167 1869 3365 425
Size: 494 248 702 1496 548
Fragments arranged by size:
1496 702 548 494 248
BsaWI w'CCGG_w
Cuts at: 677 1198 1876 2150 2297 2708 677
Size: 521 678 274 147 411 1457
Fragments arranged by size:
1457 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 376 406 376
Size: 30 3458
BseYI C'CCAG_C
Cuts at: 913 1671 2248 2744 913
Size: 758 577 496 1657
Fragments arranged by size:
1657 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1030 1076 2284 104
Size: 926 46 1208 1308
Fragments arranged by size:
1308 1208 926 46
BsiHKAI G_wGCw'C
Cuts at: 451 502 1280 1470 2262 451
Size: 51 778 190 792 1677
Fragments arranged by size:
1677 792 778 190 51
BsmI GAATG_Cn'
Cuts at: 206 206
Size: 3488
Bsp1286I G_dGCh'C
Cuts at: 116 451 502 1113 1206 1280 1470 1532 2262
Size: 335 51 611 93 74 190 62 730
Cuts at: 2262 116
Size: 1342
Fragments arranged by size:
1342 730 611 335 190 93 74 62
51
BspEI T'CCGG_A
Cuts at: 2708 2708
Size: 3488
BspHI T'CATG_A
Cuts at: 2664 2664
Size: 3488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 538 752 1054 1435 538
Size: 214 302 381 2591
Fragments arranged by size:
2591 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1780 1834 3318 3483 50
Size: 1730 54 1484 165 55
Fragments arranged by size:
1730 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1400 1400
Size: 3488
BsrFI r'CCGG_y
Cuts at: 1486 1667 1876 1486
Size: 181 209 3098
Fragments arranged by size:
3098 209 181
BssSI C'ACGA_G
Cuts at: 1759 2117 1759
Size: 358 3130
BstAPI GCAn_nnn'nTGC
Cuts at: 865 865
Size: 3488
BstBI TT'CG_AA
Cuts at: 1849 1849
Size: 3488
BstYI r'GATC_y
Cuts at: 1 513 980 1003 1338 1584 2596 2682 2694
Size: 512 467 23 335 246 1012 86 12
Cuts at: 2694 3040 1
Size: 346 449
Fragments arranged by size:
1012 512 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 343 107
Size: 236 3252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1438 1619 1782 1438
Size: 181 163 3144
Fragments arranged by size:
3144 181 163
BtsI GCAGTG_nn'
Cuts at: 459 731 459
Size: 272 3216
DraI TTT'AAA
Cuts at: 487 487
Size: 3488
DraIII CAC_nnn'GTG
Cuts at: 291 409 291
Size: 118 3370
DrdI GACnn_nn'nnGTC
Cuts at: 1194 2052 1194
Size: 858 2630
EaeI y'GGCC_r
Cuts at: 375 465 1073 1247 1638 1665 3010 375
Size: 90 608 174 391 27 1345 853
Fragments arranged by size:
1345 853 608 391 174 90 27
EagI C'GGCC_G
Cuts at: 1073 1073
Size: 3488
EarI CTCTTCn'nnn_
Cuts at: 1511 1721 1511
Size: 210 3278
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1696 2006 2152 3298 1696
Size: 310 146 1146 1886
Fragments arranged by size:
1886 1146 310 146
EcoICRI GAG'CTC
Cuts at: 500 500
Size: 3488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1313 1745 2492 2760 1313
Size: 432 747 268 2041
Fragments arranged by size:
2041 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 330 627 330
Size: 297 3191
EcoRI G'AATT_C
Cuts at: 492 492
Size: 3488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1507 1507
Size: 3488
FspI TGC'GCA
Cuts at: 1269 1269
Size: 3488
HaeII r_GCGC'y
Cuts at: 922 1170 2192 3171 922
Size: 248 1022 979 1239
Fragments arranged by size:
1239 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1330 1360 1362 1392 1330
Size: 30 2 30 3426
Fragments arranged by size:
3426 30 30 2
HincII GTy'rAC
Cuts at: 273 527 273
Size: 254 3234
KasI G'GCGC_C
Cuts at: 918 1166 918
Size: 248 3240
KpnI G_GTAC'C
Cuts at: 508 508
Size: 3488
MluI A'CGCG_T
Cuts at: 218 2921 218
Size: 2703 785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2343 2159
Size: 184 3304
MscI TGG'CCA
Cuts at: 1249 1249
Size: 3488
MslI CAynn'nnrTG
Cuts at: 1477 1604 1477
Size: 127 3361
MspA1I CmG'CkG
Cuts at: 260 345 473 599 913 1273 2286 2531 260
Size: 85 128 126 314 360 1013 245 1217
Fragments arranged by size:
1217 1013 360 314 245 128 126 85
NaeI GCC'GGC
Cuts at: 1669 1669
Size: 3488
NarI GG'CG_CC
Cuts at: 919 1167 919
Size: 248 3240
NcoI C'CATG_G
Cuts at: 107 107
Size: 3488
NgoMIV G'CCGG_C
Cuts at: 1667 1667
Size: 3488
NspI r_CATG'y
Cuts at: 177 541 1041 1948 177
Size: 364 500 907 1717
Fragments arranged by size:
1717 907 500 364
PciI A'CATG_T
Cuts at: 1944 1944
Size: 3488
PmeI GTTT'AAAC
Cuts at: 487 487
Size: 3488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 376 408 2627 2659 376
Size: 32 2219 32 1205
Fragments arranged by size:
2219 1205 32 32
PpuMI rG'GwC_Cy
Cuts at: 330 330
Size: 3488
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 220 252 220
Size: 32 3456
PstI C_TGCA'G
Cuts at: 535 1220 535
Size: 685 2803
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3488
PvuII CAG'CTG
Cuts at: 260 913 1273 260
Size: 653 360 2475
Fragments arranged by size:
2475 653 360
RsrII CG'GwC_CG
Cuts at: 347 1683 347
Size: 1336 2152
SacI G_AGCT'C
Cuts at: 502 502
Size: 3488
SacII CC_GC'GG
Cuts at: 346 346
Size: 3488
SalI G'TCGA_C
Cuts at: 525 525
Size: 3488
SapI GCTCTTCn'nnn_
Cuts at: 1511 1721 1511
Size: 210 3278
SbfI CC_TGCA'GG
Cuts at: 535 535
Size: 3488
ScaI AGT'ACT
Cuts at: 3005 3005
Size: 3488
SfcI C'TryA_G
Cuts at: 33 531 1216 2209 2400 33
Size: 498 685 993 191 1121
Fragments arranged by size:
1121 993 685 498 191
SfoI GGC'GCC
Cuts at: 920 1168 920
Size: 248 3240
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3488
SmaI CCC'GGG
Cuts at: 510 510
Size: 3488
SmlI C'TyrA_G
Cuts at: 643 1558 2050 2312 643
Size: 915 492 262 1819
Fragments arranged by size:
1819 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3488
SphI G_CATG'C
Cuts at: 541 1041 541
Size: 500 2988
StyI C'CwwG_G
Cuts at: 107 185 622 976 107
Size: 78 437 354 2619
Fragments arranged by size:
2619 437 354 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 364 493 1071 1204 1663 1872 364
Size: 129 578 133 459 209 1980
Fragments arranged by size:
1980 578 459 209 133 129
TatI w'GTAC_w
Cuts at: 3003 3003
Size: 3488
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 346 378 3005 3037 346
Size: 32 2627 32 797
Fragments arranged by size:
2627 797 32 32
Tth111I GACn'n_nGTC
Cuts at: 1285 1285
Size: 3488
XbaI T'CTAG_A
Cuts at: 519 519
Size: 3488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 983 983
Size: 3488
XmaI C'CCGG_G
Cuts at: 508 508
Size: 3488
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BpmI Bpu10I BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI