Restriction map for
pF1KB7514
(Circular) (Six-base) MAPSORT of: pF1KB7514.seq Check: 6377 from: 1 to: 3848
pF1KB7514 3848 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 886 886
Size: 3848
Acc65I G'GTAC_C
Cuts at: 864 864
Size: 3848
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1673 2105 2852 3120 1673
Size: 432 747 268 2401
Fragments arranged by size:
2401 747 432 268
AflIII A'CryG_T
Cuts at: 1200 2304 3281 1200
Size: 1104 977 1767
Fragments arranged by size:
1767 1104 977
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 799 799
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 301 561 827 1153 2720 301
Size: 260 266 326 1567 1429
Fragments arranged by size:
1567 1429 326 266 260
ApaI G_GGCC'C
Cuts at: 127 277 611 127
Size: 150 334 3364
Fragments arranged by size:
3364 334 150
ApaLI G'TGCA_C
Cuts at: 2618 2618
Size: 3848
ApoI r'AATT_y
Cuts at: 852 3201 3457 852
Size: 2349 256 1243
Fragments arranged by size:
2349 1243 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 479 868 479
Size: 389 3459
BamHI G'GATC_C
Cuts at: 873 873
Size: 3848
BanI G'GyrC_C
Cuts at: 864 1278 1526 1561 864
Size: 414 248 35 3151
Fragments arranged by size:
3151 414 248 35
BanII G_rGCy'C
Cuts at: 127 277 552 611 862 1892 127
Size: 150 275 59 251 1030 2083
Fragments arranged by size:
2083 1030 275 251 150 59
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BbsI GAAGACnn'nnnn_
Cuts at: 748 748
Size: 3848
BbvCI CC'TCA_GC
Cuts at: 116 116
Size: 3848
BciVI GTATCCnnnnn_n'
Cuts at: 1746 2507 3848 1746
Size: 761 1341 1746
Fragments arranged by size:
1746 1341 761
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BfrBI ATG'CAT
Cuts at: 791 791
Size: 3848
BglI GCCn_nnn'nGGC
Cuts at: 577 577
Size: 3848
BglII A'GATC_T
Cuts at: 404 488 1363 3400 404
Size: 84 875 2037 852
Fragments arranged by size:
2037 875 852 84
BlpI GC'TnA_GC
Cuts at: 187 960 187
Size: 773 3075
Bme1580I G_kGCm'C
Cuts at: 127 277 611 1473 1566 2622 127
Size: 150 334 862 93 1056 1353
Fragments arranged by size:
1353 1056 862 334 150 93
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1141 1476 3620 1060
Size: 81 335 2144 1288
Fragments arranged by size:
2144 1288 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 3848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 3848
Bpu10I CC'TnA_GC
Cuts at: 116 116
Size: 3848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 1024 1903 2395 2693 656
Size: 368 879 492 298 1811
Fragments arranged by size:
1811 879 492 368 298
BsaI GGTCTCn'nnnn_
Cuts at: 3552 3552
Size: 3848
BsaAI yAC'GTr
Cuts at: 614 1201 614
Size: 587 3261
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 1279 1527 2229 3725 1279
Size: 248 702 1496 1402
Fragments arranged by size:
1496 1402 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1558 2236 2510 2657 3068 1037
Size: 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 348 378 348
Size: 30 3818
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 249 635 706 249
Size: 386 71 3391
Fragments arranged by size:
3391 386 71
BseYI C'CCAG_C
Cuts at: 604 720 1273 2031 2608 3104 604
Size: 116 553 758 577 496 1348
Fragments arranged by size:
1348 758 577 553 496 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 505
Size: 3848
BsiEI CG_ry'CG
Cuts at: 104 1390 1436 2644 104
Size: 1286 46 1208 1308
Fragments arranged by size:
1308 1286 1208 46
BsiHKAI G_wGCw'C
Cuts at: 862 1640 1830 2622 862
Size: 778 190 792 2088
Fragments arranged by size:
2088 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 127 277 552 611 862 1473 1566 1640 1830
Size: 150 275 59 251 611 93 74 190
Cuts at: 1830 1892 2622 127
Size: 62 730 1353
Fragments arranged by size:
1353 730 611 275 251 190 150 93
74 62 59
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 898 1112 1414 1795 898
Size: 214 302 381 2951
Fragments arranged by size:
2951 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2140 2194 3678 3843 50
Size: 2090 54 1484 165 55
Fragments arranged by size:
2090 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1760 1760
Size: 3848
BsrFI r'CCGG_y
Cuts at: 1846 2027 2236 1846
Size: 181 209 3458
Fragments arranged by size:
3458 209 181
BssSI C'ACGA_G
Cuts at: 651 2119 2477 651
Size: 1468 358 2022
Fragments arranged by size:
2022 1468 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstXI CCAn_nnnn'nTGG
Cuts at: 594 594
Size: 3848
BstYI r'GATC_y
Cuts at: 1 404 488 873 1340 1363 1698 1944 2956
Size: 403 84 385 467 23 335 246 1012
Cuts at: 2956 3042 3054 3400 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 403 385 346 335 246
86 84 23 12
Bsu36I CC'TnA_GG
Cuts at: 294 294
Size: 3848
BtgI C'CryG_G
Cuts at: 730 730
Size: 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 623 1091 623
Size: 468 3380
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 1433 1607 1998 2025 3370 1433
Size: 174 391 27 1345 1911
Fragments arranged by size:
1911 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 263 340 1871 2081 263
Size: 77 1531 210 2030
Fragments arranged by size:
2030 1531 210 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 534 2056 2366 2512 3658 534
Size: 1522 310 146 1146 724
Fragments arranged by size:
1522 1146 724 310 146
EcoICRI GAG'CTC
Cuts at: 860 860
Size: 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1673 2105 2852 3120 418
Size: 1255 432 747 268 1146
Fragments arranged by size:
1255 1146 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 749 749
Size: 3848
EcoO109I rG'GnC_Cy
Cuts at: 124 174 273 274 987 124
Size: 50 99 1 713 2985
Fragments arranged by size:
2985 713 99 50 1
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3848
FspI TGC'GCA
Cuts at: 634 1629 634
Size: 995 2853
HaeII r_GCGC'y
Cuts at: 1282 1530 2552 3531 1282
Size: 248 1022 979 1599
Fragments arranged by size:
1599 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 645 677 1690 1720 1722 1752 645
Size: 32 1013 30 2 30 2741
Fragments arranged by size:
2741 1013 32 30 30 2
HincII GTy'rAC
Cuts at: 887 887
Size: 3848
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 868 868
Size: 3848
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2519 2703 2519
Size: 184 3664
MscI TGG'CCA
Cuts at: 1609 1609
Size: 3848
MslI CAynn'nnrTG
Cuts at: 781 1837 1964 781
Size: 1056 127 2665
Fragments arranged by size:
2665 1056 127
MspA1I CmG'CkG
Cuts at: 387 450 561 681 959 1273 1633 2646 2891
Size: 63 111 120 278 314 360 1013 245
Cuts at: 2891 387
Size: 1344
Fragments arranged by size:
1344 1013 360 314 278 245 120 111
63
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 730 730
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NsiI A_TGCA'T
Cuts at: 793 793
Size: 3848
NspI r_CATG'y
Cuts at: 901 1401 2308 901
Size: 500 907 2441
Fragments arranged by size:
2441 907 500
PciI A'CATG_T
Cuts at: 2304 2304
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PmlI CAC'GTG
Cuts at: 614 614
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PspOMI G'GGCC_C
Cuts at: 123 273 607 123
Size: 150 334 3364
Fragments arranged by size:
3364 334 150
PstI C_TGCA'G
Cuts at: 139 895 1580 139
Size: 756 685 2407
Fragments arranged by size:
2407 756 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 387 450 561 681 1273 1633 387
Size: 63 111 120 592 360 2602
Fragments arranged by size:
2602 592 360 120 111 63
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 862 862
Size: 3848
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 340 1871 2081 340
Size: 1531 210 2107
Fragments arranged by size:
2107 1531 210
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SfcI C'TryA_G
Cuts at: 33 135 891 1576 2569 2760 33
Size: 102 756 685 993 191 1121
Fragments arranged by size:
1121 993 756 685 191 102
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 870 870
Size: 3848
SmlI C'TyrA_G
Cuts at: 671 1003 1918 2410 2672 671
Size: 332 915 492 262 1847
Fragments arranged by size:
1847 915 492 332 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
StuI AGG'CCT
Cuts at: 225 225
Size: 3848
StyI C'CwwG_G
Cuts at: 730 982 1336 730
Size: 252 354 3242
Fragments arranged by size:
3242 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 743 1431 1564 2023 2232 743
Size: 688 133 459 209 2359
Fragments arranged by size:
2359 688 459 209 133
TatI w'GTAC_w
Cuts at: 3363 3363
Size: 3848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 731 763 3365 3397 731
Size: 32 2602 32 1182
Fragments arranged by size:
2602 1182 32 32
Tth111I GACn'n_nGTC
Cuts at: 1645 1645
Size: 3848
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 728 812 1343 728
Size: 84 531 3233
Fragments arranged by size:
3233 531 84
XmaI C'CCGG_G
Cuts at: 868 868
Size: 3848
XmnI GAAnn'nnTTC
Cuts at: 406 406
Size: 3848
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI