Restriction map for
pF1KB7515
(Circular) (Six-base) MAPSORT of: pF1KB7515.seq Check: 9548 from: 1 to: 3494
pF1KB7515 3494 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 532 532
Size: 3494
Acc65I G'GTAC_C
Cuts at: 510 510
Size: 3494
AclI AA'CG_TT
Cuts at: 3334 3334
Size: 3494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 412 1319 1751 2498 2766 391
Size: 21 907 432 747 268 1119
Fragments arranged by size:
1119 907 747 432 268 21
AflIII A'CryG_T
Cuts at: 846 1950 2927 846
Size: 1104 977 1413
Fragments arranged by size:
1413 1104 977
AgeI A'CCGG_T
Cuts at: 1882 1882
Size: 3494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 157 157
Size: 3494
AlwNI CAG_nnn'CTG
Cuts at: 799 2366 799
Size: 1567 1927
ApaLI G'TGCA_C
Cuts at: 2264 2264
Size: 3494
ApoI r'AATT_y
Cuts at: 498 2847 3103 498
Size: 2349 256 889
Fragments arranged by size:
2349 889 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3494
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
AvaI C'yCGr_G
Cuts at: 163 485 514 163
Size: 322 29 3143
Fragments arranged by size:
3143 322 29
BamHI G'GATC_C
Cuts at: 519 519
Size: 3494
BanI G'GyrC_C
Cuts at: 159 510 924 1172 1207 159
Size: 351 414 248 35 2446
Fragments arranged by size:
2446 414 351 248 35
BanII G_rGCy'C
Cuts at: 122 202 508 1538 122
Size: 80 306 1030 2078
Fragments arranged by size:
2078 1030 306 80
BbeI G_GCGC'C
Cuts at: 163 928 1176 163
Size: 765 248 2481
Fragments arranged by size:
2481 765 248
BciVI GTATCCnnnnn_n'
Cuts at: 1392 2153 3494 1392
Size: 761 1341 1392
Fragments arranged by size:
1392 1341 761
BclI T'GATC_A
Cuts at: 1014 1014
Size: 3494
BglI GCCn_nnn'nGGC
Cuts at: 456 456
Size: 3494
BglII A'GATC_T
Cuts at: 1009 3046 1009
Size: 2037 1457
BlpI GC'TnA_GC
Cuts at: 606 606
Size: 3494
Bme1580I G_kGCm'C
Cuts at: 415 1119 1212 2268 415
Size: 704 93 1056 1641
Fragments arranged by size:
1641 1056 704 93
BmrI ACTGGGnnnn_n'
Cuts at: 706 787 1122 3266 706
Size: 81 335 2144 934
Fragments arranged by size:
2144 934 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 337
Size: 3494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1549 2041 2339 670
Size: 879 492 298 1825
Fragments arranged by size:
1825 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3198 3198
Size: 3494
BsaAI yAC'GTr
Cuts at: 847 847
Size: 3494
BsaBI GATnn'nnATC
Cuts at: 6 1008 3045 6
Size: 1002 2037 455
Fragments arranged by size:
2037 1002 455
BsaHI Gr'CG_yC
Cuts at: 160 925 1173 1875 3371 160
Size: 765 248 702 1496 283
Fragments arranged by size:
1496 765 702 283 248
BsaWI w'CCGG_w
Cuts at: 683 1204 1882 2156 2303 2714 683
Size: 521 678 274 147 411 1463
Fragments arranged by size:
1463 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 162 379 162
Size: 217 3277
BseYI C'CCAG_C
Cuts at: 919 1677 2254 2750 919
Size: 758 577 496 1663
Fragments arranged by size:
1663 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 128 460 1036 1082 2290 104
Size: 24 332 576 46 1208 1308
Fragments arranged by size:
1308 1208 576 332 46 24
BsiHKAI G_wGCw'C
Cuts at: 401 508 1286 1476 2268 401
Size: 107 778 190 792 1627
Fragments arranged by size:
1627 792 778 190 107
Bsp1286I G_dGCh'C
Cuts at: 122 202 401 415 508 1119 1212 1286 1476
Size: 80 199 14 93 611 93 74 190
Cuts at: 1476 1538 2268 122
Size: 62 730 1348
Fragments arranged by size:
1348 730 611 199 190 93 93 80
74 62 14
BspEI T'CCGG_A
Cuts at: 2714 2714
Size: 3494
BspHI T'CATG_A
Cuts at: 2670 2670
Size: 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 544 758 1060 1441 544
Size: 214 302 381 2597
Fragments arranged by size:
2597 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1786 1840 3324 3489 50
Size: 1736 54 1484 165 55
Fragments arranged by size:
1736 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1406 1406
Size: 3494
BsrFI r'CCGG_y
Cuts at: 130 472 1492 1673 1882 130
Size: 342 1020 181 209 1742
Fragments arranged by size:
1742 1020 342 209 181
BssSI C'ACGA_G
Cuts at: 400 1765 2123 400
Size: 1365 358 1771
Fragments arranged by size:
1771 1365 358
BstAPI GCAn_nnn'nTGC
Cuts at: 871 871
Size: 3494
BstBI TT'CG_AA
Cuts at: 1855 1855
Size: 3494
BstYI r'GATC_y
Cuts at: 1 519 986 1009 1344 1590 2602 2688 2700
Size: 518 467 23 335 246 1012 86 12
Cuts at: 2700 3046 1
Size: 346 449
Fragments arranged by size:
1012 518 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 203 203
Size: 3494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1444 1625 1788 1444
Size: 181 163 3150
Fragments arranged by size:
3150 181 163
BtsI GCAGTG_nn'
Cuts at: 737 737
Size: 3494
DraI TTT'AAA
Cuts at: 493 493
Size: 3494
DrdI GACnn_nn'nnGTC
Cuts at: 220 1200 2058 220
Size: 980 858 1656
Fragments arranged by size:
1656 980 858
EaeI y'GGCC_r
Cuts at: 125 457 470 1079 1253 1644 1671 3016 125
Size: 332 13 609 174 391 27 1345 603
Fragments arranged by size:
1345 609 603 391 332 174 27 13
EagI C'GGCC_G
Cuts at: 125 457 1079 125
Size: 332 622 2540
Fragments arranged by size:
2540 622 332
EarI CTCTTCn'nnn_
Cuts at: 1517 1727 1517
Size: 210 3284
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1702 2012 2158 3304 1702
Size: 310 146 1146 1892
Fragments arranged by size:
1892 1146 310 146
EcoICRI GAG'CTC
Cuts at: 506 506
Size: 3494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 391 412 1319 1751 2498 2766 337
Size: 54 21 907 432 747 268 1065
Fragments arranged by size:
1065 907 747 432 268 54 21
EcoNI CCTnn'n_nnAGG
Cuts at: 382 382
Size: 3494
EcoO109I rG'GnC_Cy
Cuts at: 633 633
Size: 3494
EcoRI G'AATT_C
Cuts at: 498 498
Size: 3494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1513 1513
Size: 3494
FspI TGC'GCA
Cuts at: 1275 1275
Size: 3494
HaeII r_GCGC'y
Cuts at: 163 928 1176 2198 3177 163
Size: 765 248 1022 979 480
Fragments arranged by size:
1022 979 765 480 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1336 1366 1368 1398 1336
Size: 30 2 30 3432
Fragments arranged by size:
3432 30 30 2
HincII GTy'rAC
Cuts at: 533 533
Size: 3494
KasI G'GCGC_C
Cuts at: 159 924 1172 159
Size: 765 248 2481
Fragments arranged by size:
2481 765 248
KpnI G_GTAC'C
Cuts at: 514 514
Size: 3494
MluI A'CGCG_T
Cuts at: 2927 2927
Size: 3494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 244 2165 2349 244
Size: 1921 184 1389
Fragments arranged by size:
1921 1389 184
MscI TGG'CCA
Cuts at: 1255 1255
Size: 3494
MslI CAynn'nnrTG
Cuts at: 1483 1610 1483
Size: 127 3367
MspA1I CmG'CkG
Cuts at: 417 605 919 1279 2292 2537 417
Size: 188 314 360 1013 245 1374
Fragments arranged by size:
1374 1013 360 314 245 188
NaeI GCC'GGC
Cuts at: 132 474 1675 132
Size: 342 1201 1951
Fragments arranged by size:
1951 1201 342
NarI GG'CG_CC
Cuts at: 160 925 1173 160
Size: 765 248 2481
Fragments arranged by size:
2481 765 248
NgoMIV G'CCGG_C
Cuts at: 130 472 1673 130
Size: 342 1201 1951
Fragments arranged by size:
1951 1201 342
NspI r_CATG'y
Cuts at: 547 1047 1954 547
Size: 500 907 2087
Fragments arranged by size:
2087 907 500
PciI A'CATG_T
Cuts at: 1950 1950
Size: 3494
PmeI GTTT'AAAC
Cuts at: 493 493
Size: 3494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2633 2665 2633
Size: 32 3462
PstI C_TGCA'G
Cuts at: 146 541 1226 146
Size: 395 685 2414
Fragments arranged by size:
2414 685 395
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3494
PvuII CAG'CTG
Cuts at: 919 1279 919
Size: 360 3134
RsrII CG'GwC_CG
Cuts at: 1689 1689
Size: 3494
SacI G_AGCT'C
Cuts at: 508 508
Size: 3494
SalI G'TCGA_C
Cuts at: 531 531
Size: 3494
SapI GCTCTTCn'nnn_
Cuts at: 1517 1727 1517
Size: 210 3284
SbfI CC_TGCA'GG
Cuts at: 541 541
Size: 3494
ScaI AGT'ACT
Cuts at: 3011 3011
Size: 3494
SfcI C'TryA_G
Cuts at: 33 142 537 1222 2215 2406 33
Size: 109 395 685 993 191 1121
Fragments arranged by size:
1121 993 685 395 191 109
SfoI GGC'GCC
Cuts at: 161 926 1174 161
Size: 765 248 2481
Fragments arranged by size:
2481 765 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
SmaI CCC'GGG
Cuts at: 165 516 165
Size: 351 3143
SmlI C'TyrA_G
Cuts at: 649 1564 2056 2318 649
Size: 915 492 262 1825
Fragments arranged by size:
1825 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3494
SphI G_CATG'C
Cuts at: 547 1047 547
Size: 500 2994
StyI C'CwwG_G
Cuts at: 628 982 628
Size: 354 3140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1077 1210 1669 1878 1077
Size: 133 459 209 2693
Fragments arranged by size:
2693 459 209 133
TatI w'GTAC_w
Cuts at: 3009 3009
Size: 3494
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3462
Tth111I GACn'n_nGTC
Cuts at: 1291 1291
Size: 3494
XbaI T'CTAG_A
Cuts at: 525 525
Size: 3494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 989 989
Size: 3494
XmaI C'CCGG_G
Cuts at: 163 514 163
Size: 351 3143
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI